Bio-Radiations https://www.bioradiations.com A Resource for Life Science Research Thu, 05 Mar 2026 20:47:39 +0000 en-US hourly 1 https://www.bioradiations.com/wp-content/uploads/2025/10/BR-knocked-out-60x60.png Bio-Radiations https://www.bioradiations.com 32 32 Each month, join Kashef Qaadri, a biologist turned bioinformatician, as he interviews guests exploring real-world research informatics challenges through use-cases, providing insights and strategies for integrating and analyzing complex data that are driving BioPharma R&D. Bio-Rad Laboratories, Inc. false Bio-Rad Laboratories, Inc. lsgmarcomapps@gmail.com Copyright © 2019 Bio-Rad Laboratories, Inc. All rights reserved. Copyright © 2019 Bio-Rad Laboratories, Inc. All rights reserved. podcast Each month, join Kashef Qaadri, a biologist turned bioinformatician, as he interviews guests exploring real-world research informatics challenges through use-cases, providing insights and strategies for integrating and analyzing complex data that are d... Bio-Radiations https://www.bioradiations.com/wp-content/uploads/powerpress/bioradio_podsq_1400x1400-400.jpg https://www.bioradiations.com From Gel to Journal: A Practical Guide to Western Blot Submission https://www.bioradiations.com/from-gel-to-journal-a-practical-guide-to-western-blot-submission/ Thu, 05 Mar 2026 20:47:39 +0000 https://www.bioradiations.com/?p=13046 If you are getting ready to submit a research paper with western blot data, you’ve probably noticed that journals have specific—and often strict—guidelines for submitting digital images. To help you avoid last-minute headaches and uncertainty over whether ...

The post From Gel to Journal: A Practical Guide to Western Blot Submission first appeared on Bio-Radiations.]]> Build Successful Panels for Spectral Flow Cytometry with These Seven Tips  https://www.bioradiations.com/build-successful-panels-for-spectral-flow-cytometry-with-these-seven-tips/ Mon, 09 Feb 2026 17:47:53 +0000 https://www.bioradiations.com/?p=13039 IT’S HERE! The core lab finally invested in a spectral flow cytometer, and you can now combine your two, 20-color panels into one large panel, potentially reducing instrument time by half. However, to build the optimal panel it is important to consider where to begin ...

The post Build Successful Panels for Spectral Flow Cytometry with These Seven Tips  first appeared on Bio-Radiations.]]> Monoclonal Antibody Purification: 
A Two-Step Approach Using a Strong AEX-HIC Mixed-Mode Resin https://www.bioradiations.com/monoclonal-antibody-purification-a-two-step-approach-using-a-strong-aex-hic-mixed-mode-resin/ Fri, 30 Jan 2026 21:45:00 +0000 https://www.bioradiations.com/?p=13028 Monoclonal antibodies (mAbs) increasingly comprise a large portion of the product pipeline of major biopharmaceutical companies. Challenges in mAb purification include reducing production cost, developing robust processes for both product purity and viral clearance, and integrating upstream ...

The post Monoclonal Antibody Purification: 
A Two-Step Approach Using a Strong AEX-HIC Mixed-Mode Resin first appeared on Bio-Radiations.]]> A Gentler Workflow: Fc-Fusion Protein Purification with Mixed-Mode and Anion Exchange Chromatography https://www.bioradiations.com/a-gentler-workflow-fc-fusion-protein-purification-with-mixed-mode-and-anion-exchange-chromatography/ Mon, 26 Jan 2026 18:42:38 +0000 https://www.bioradiations.com/?p=13020 Traditional Protein A affinity chromatography is the gold standard for Fc-fusion protein purification, but protein denaturation and degradation due to low-pH elution are limitations. This webcast presents a proof-of-concept study demonstrating the use of ...

The post A Gentler Workflow: Fc-Fusion Protein Purification with Mixed-Mode and Anion Exchange Chromatography first appeared on Bio-Radiations.]]> qPCR vs. ddPCR™ Assays: Select the Best Gene Expression Analysis Method for Your Application https://www.bioradiations.com/qpcr-vs-ddpcr-assays-select-the-best-gene-expression-analysis-method-for-your-application1125/ Tue, 11 Nov 2025 17:57:13 +0000 https://www.bioradiations.com/?p=12923 Unsure whether to choose quantitative PCR (qPCR) or Droplet Digital™ PCR (ddPCR) for gene expression analysis? Bio-Rad’s new objective study comparing the two methods shows that the best choice depends ...

The post qPCR vs. ddPCR™ Assays: Select the Best Gene Expression Analysis Method for Your Application first appeared on Bio-Radiations.]]> Stain-Free Technology: Get Better Data in Less Time https://www.bioradiations.com/stain-free-technology-get-better-data-in-less-time1125/ Tue, 11 Nov 2025 17:56:32 +0000 https://www.bioradiations.com/?p=12934 Western blotting is a cornerstone of protein analysis—but the traditional workflow is slow, labor-intensive, and error-prone, plus it requires staining steps to visualize proteins on a gel or blot. Stain-Free ...

The post Stain-Free Technology: Get Better Data in Less Time first appeared on Bio-Radiations.]]> Real-World AAV Empty-Full Capsid Analysis with ddPCR Technology: Insights from Matica Biotechnology https://www.bioradiations.com/real-world-aav-empty-full-capsid-analysis-with-ddpcr-technology-insights-from-matica-biotechnology1125/ Tue, 11 Nov 2025 17:55:56 +0000 https://www.bioradiations.com/?p=12940 Vericheck ddPCR Empty-Full Capsid Kits for AAV Workflows Measuring the ratio of empty to full capsids serves as a critical quality attribute (CQA) for adeno-associated virus (AAV) products and directly ...

The post Real-World AAV Empty-Full Capsid Analysis with ddPCR Technology: Insights from Matica Biotechnology first appeared on Bio-Radiations.]]> 10 Tips for Optimizing High-Throughput Flow Cytometry Assays https://www.bioradiations.com/10-tips-for-optimizing-high-throughput-flow-cytometry-assays1125/ Tue, 11 Nov 2025 17:55:05 +0000 https://www.bioradiations.com/?p=12917 Flow cytometry can be labor intensive, and efforts to increase throughput can lead to reduced data quality or even instrument reliability issues such as blockages. While some instruments are more ...

The post 10 Tips for Optimizing High-Throughput Flow Cytometry Assays first appeared on Bio-Radiations.]]> Evaluation of A New Multimodal Anion Exchange Resin for Emerging Biotherapeutic Process Challenges https://www.bioradiations.com/evaluation-of-a-new-multimodal-anion-exchange-resin-for-emerging-biotherapeutic-process-challenges1125/ Tue, 11 Nov 2025 17:54:04 +0000 https://www.bioradiations.com/?p=12914 Multimodal chromatography has a history of providing value for biopharmaceutical separations. Ligands that enable binding based on both charge and hydrophobic interactions provide more opportunity for effective separation of multiple ...

The post Evaluation of A New Multimodal Anion Exchange Resin for Emerging Biotherapeutic Process Challenges first appeared on Bio-Radiations.]]> Optimize Your Assays for High-Throughput Flow Cytometry https://www.bioradiations.com/optimize-your-assays-for-high-throughput-flow-cytometry1125/ Tue, 11 Nov 2025 17:53:11 +0000 https://www.bioradiations.com/?p=12910 Flow cytometry is a powerful technique with many applications in both academia and drug discovery. It can facilitate complex immunophenotyping experiments, making it an indispensable tool for understanding biological systems. ...

The post Optimize Your Assays for High-Throughput Flow Cytometry first appeared on Bio-Radiations.]]> BioRad.io Podcast Series https://www.bioradiations.com/bioradio/ Mon, 10 Nov 2025 22:03:24 +0000 https://www.bioradiations.com/?p=11299 The BioRad.io podcast series is focused on R&D informatics, introducing you to people responsible for implementing systems and technologies to a diverse set of use-cases. Join us every month to see what you can learn.

The post BioRad.io Podcast Series first appeared on Bio-Radiations.]]> Advancements in CMOS Sensors for Scientific Imaging https://www.bioradiations.com/advancements-in-cmos-sensors-for-scientific-imaging-525/ Tue, 13 May 2025 18:22:04 +0000 https://www.bioradiations.com/?p=12775 For years, CCD sensors, known for their high-quality imaging capabilities, have been the gold standard in scientific instrumentation. However, recent advancements in CMOS sensors have positioned them as a cost-effective and competitive alternative that is quickly gaining traction in the field. In this article, we delve into the strengths and applications of CCD and CMOS sensors, exploring how this shift in technology is reshaping the digital imaging landscape.

The post Advancements in CMOS Sensors for Scientific Imaging first appeared on Bio-Radiations.]]> As Easy as 1, 2, 3: Achieving Purification Success with a New Two-Mode Chromatography Resin https://www.bioradiations.com/purification-success-with-new-weak-aex-hic-resin-525/ Tue, 13 May 2025 18:21:43 +0000 https://www.bioradiations.com/?p=12778 A common misconception is that stronger is always better. However, Nuvia wPrime 2A Media, a weak anion exchange hydrophobic interaction (AEX-HIC) mixed-mode chromatography resin, has won over purification scientists. Read about diverse use cases for Nuvia wPrime 2A Media and see the data for yourself.

The post As Easy as 1, 2, 3: Achieving Purification Success with a New Two-Mode Chromatography Resin first appeared on Bio-Radiations.]]> Bio-Rad’s Downstream Process Development Support and Services https://www.bioradiations.com/bio-rads-downstream-process-support-services-525/ Tue, 13 May 2025 18:21:00 +0000 https://www.bioradiations.com/?p=12760 Bio-Rad’s Chromatography Support Scientist, Artur Stanczak, discusses the company’s comprehensive downstream process development services. From method development to troubleshooting, Bio-Rad provides specialized support to help customers overcome bioprocessing challenges. Access tools, case studies, and expert guidance to optimize processes and advance biomanufacturing efforts in this informative video.

The post Bio-Rad’s Downstream Process Development Support and Services first appeared on Bio-Radiations.]]> Navigating Change: Public Health Labs Are Adapting to New Regulations for Laboratory-Developed Tests https://www.bioradiations.com/public-health-labs-adapt-to-ldt-regulations-525/ Tue, 13 May 2025 18:20:26 +0000 https://www.bioradiations.com/?p=12772 Labs face a growing compliance burden and increased uncertainty due to new regulations surrounding laboratory-developed tests (LDTs). The U.S. FDA's final rule and Supreme Court's recent decision to overturn the Chevron doctrine are creating a complex regulatory environment that challenges the ability of public health labs to rapidly respond to emerging pathogens. This article explores the implications of these changes and more.

The post Navigating Change: Public Health Labs Are Adapting to New Regulations for Laboratory-Developed Tests first appeared on Bio-Radiations.]]> Compliance and Regulations for Flow Cytometry in Drug Discovery: Webinar Series https://www.bioradiations.com/compliance-and-regulations-for-flow-cytometry-in-drug-discovery-webinar-series-525/ Tue, 13 May 2025 18:18:38 +0000 https://www.bioradiations.com/?p=12755 In this webinar series, learn about 21 CFR Part 11 regulations for flow cytometry in drug discovery. Learn what software and instruments can help you comply.

The post Compliance and Regulations for Flow Cytometry in Drug Discovery: Webinar Series first appeared on Bio-Radiations.]]> Demonstrating a Modular Selectivity with a Weak AEX-HIC Resin for Achieving High mAb Purity and Recovery in a Downstream Process https://www.bioradiations.com/modular-selectivity-with-aex-hic-resin-for-mab-purity-525/ Tue, 13 May 2025 18:18:19 +0000 https://www.bioradiations.com/?p=12753 Learn how Bio-Rad's Nuvia wPrime 2A Media, a weak AEX-HIC resin, is used in an efficient monoclonal antibody (mAb) purification process. Presented by Dr. Sushmita Koley, this webcast demonstrates how multimodal chromatography improves purity and recovery, achieving a 10x reduction in high molecular weight species while maintaining 85% mAb monomer recovery. Watch to explore innovative solutions for biomanufacturing.

The post Demonstrating a Modular Selectivity with a Weak AEX-HIC Resin for Achieving High mAb Purity and Recovery in a Downstream Process first appeared on Bio-Radiations.]]> Saving Lives: Innovative Therapeutic Applications of Ion Exchange Resins https://www.bioradiations.com/therapeutic-applications-of-ion-exchange-resins-225/ Tue, 11 Feb 2025 20:39:18 +0000 https://www.bioradiations.com/?p=12722 Although chromatography resins are usually thought of as tools to purify medicines, groundbreaking treatments now utilize resin beads as an integral component of the therapy itself. These therapies are essential for treating certain cancers and inflammatory disorders.

The post Saving Lives: Innovative Therapeutic Applications of Ion Exchange Resins first appeared on Bio-Radiations.]]> The Journey from Lab to Clinic: Zolgensma, the First Gene Therapy Treatment for Spinal Muscular Atrophy (SMA) https://www.bioradiations.com/lab-to-clinic-the-first-gene-therapy-for-sma-225/ Tue, 11 Feb 2025 20:16:49 +0000 https://www.bioradiations.com/?p=12720 Translational research bridges the gap between scientific discoveries and real-world applications, transforming scientific breakthroughs into life-changing therapies. Success in this endeavor hinges on several factors, including collaboration between scientists and clinicians. A notable example of this is the approval of Zolgensma, a gene therapy for spinal muscular atrophy (SMA), which marked a significant milestone in the field of translational research.

The post The Journey from Lab to Clinic: Zolgensma, the First Gene Therapy Treatment for Spinal Muscular Atrophy (SMA) first appeared on Bio-Radiations.]]> Vive la Différence: The Beauty of Diversity in Life, Life Science, and Flow Cytometry https://www.bioradiations.com/the-beauty-of-diversity-in-flow-cytometry-225/ Tue, 11 Feb 2025 19:23:13 +0000 https://www.bioradiations.com/?p=12712 The differences between us are what makes life interesting, and diversity in a group that shares a common goal can lead to many benefits. Here we examine the similarities and differences between researchers using flow cytometry for academic research and those using it for drug discovery. We outline the challenges they face and learn that they share a common goal as well as a common solution.

The post Vive la Différence: The Beauty of Diversity in Life, Life Science, and Flow Cytometry first appeared on Bio-Radiations.]]> Compliance at Your Fingertips: Using Image Lab Touch Software for Digital Imaging in Regulated Environments https://www.bioradiations.com/ensuring-compliance-in-digital-imaging-labs-225/ Tue, 11 Feb 2025 19:00:00 +0000 https://www.bioradiations.com/?p=12705 Maintaining compliance while leveraging advanced digital imaging technologies can be a daunting task. Luckily, Bio-Rad Image Lab Touch Software takes this challenge head-on by offering a seamless solution for digital imaging. Learn how Image Lab Touch Software integrates with Bio-Rad imaging systems to support researchers in meeting stringent regulatory requirements, ensuring data integrity and security without compromising efficiency or ease of use.

The post Compliance at Your Fingertips: Using Image Lab Touch Software for Digital Imaging in Regulated Environments first appeared on Bio-Radiations.]]> Global Lung Cancer Expert Dr. Ed Schuuring on How ddPCR Technology Powers ctDNA Analysis https://www.bioradiations.com/how-ddpcr-technology-powers-ctdna-analysis-225/ Tue, 11 Feb 2025 18:43:53 +0000 https://www.bioradiations.com/?p=12703 Characterizing biomarkers in lung cancer tumors is challenging due to the high risks associated with tissue retrieval. Liquid biopsy offers a safer alternative but requires highly sensitive and precise methods to generate robust results from minimal genetic material. In this video, Dr. Schuuring of the University Medical Center Groningen discusses how Droplet Digital PCR (ddPCR) is used in his laboratories and why this technology has become critical for his research.

The post Global Lung Cancer Expert Dr. Ed Schuuring on How ddPCR Technology Powers ctDNA Analysis first appeared on Bio-Radiations.]]> Biotherapeutic Antibody Discovery at Bio-Rad’s New Center for Excellence https://www.bioradiations.com/bio-rads-new-center-for-antibody-discovery-225/ Tue, 11 Feb 2025 18:19:21 +0000 https://www.bioradiations.com/?p=12693 The Pioneer Platform features one of the largest functional antibody libraries available, utilizing SpyLock technology for rapid prototyping and screening of hundreds of bispecific antibodies, and TrailBlazer technology for efficient antibody format conversion. Together, these services provide a comprehensive and streamlined solution for researchers seeking to develop innovative biotherapeutic therapies.

The post Biotherapeutic Antibody Discovery at Bio-Rad’s New Center for Excellence first appeared on Bio-Radiations.]]> Maximizing PCR and qPCR Throughput with Automated Solutions https://www.bioradiations.com/automated-pcr-and-qpcr-solutions-225/ Tue, 11 Feb 2025 18:10:48 +0000 https://www.bioradiations.com/?p=12697 Automation in PCR and qPCR processes is revolutionizing laboratory workflows in regulated environments like biopharma and CROs. By enhancing efficiency, reducing manual errors, and ensuring compliance with regulatory standards, automation allows labs to scale operations and increase throughput without compromising data quality. Here we show the range of Bio-Rad products that are compatible with automated solutions for PCR and qPCR workflows.

The post Maximizing PCR and qPCR Throughput with Automated Solutions first appeared on Bio-Radiations.]]> Development of an IEX Purification Process for Lentiviral Vectors https://www.bioradiations.com/iex-purification-process-for-lentiviral-vectors-225/ Tue, 11 Feb 2025 18:03:46 +0000 https://www.bioradiations.com/?p=12691 In recent years, gene therapy treatments have increased significantly, requiring more efficient processes to purify and deliver these therapeutics. Lentiviral vectors (LVVs) are a type of retrovirus and are an effective tool for gene delivery. In this webinar, data are shown for a high-throughput screening strategy that was employed for LVV purification, which provided high yield as well as acceptable host cell protein (HCP) clearance.

The post Development of an IEX Purification Process for Lentiviral Vectors first appeared on Bio-Radiations.]]> Evaluation of a New Multimodal Anion Exchange Resin for Emerging Biotherapeutic Process Challenges https://www.bioradiations.com/nuvia-wprime-2a-resin-has-mmaex-functionality-with-a-weak-anion-exchanger-see-data-showing-improved-mab-and-bsab-purification-compared-to-traditional-resins-225/ Tue, 11 Feb 2025 17:59:48 +0000 https://www.bioradiations.com/?p=12687 Nuvia wPrime 2A Resin has MMAEX functionality with a weak anion exchanger. See data showing improved mAb and bsAb purification compared to traditional resins.

The post Evaluation of a New Multimodal Anion Exchange Resin for Emerging Biotherapeutic Process Challenges first appeared on Bio-Radiations.]]> Cytometry in Drug Discovery: An Introduction to Regulation, Standardization, and Productivity https://www.bioradiations.com/cytometry-in-drug-discovery-an-introduction-225/ Tue, 11 Feb 2025 17:40:52 +0000 https://www.bioradiations.com/?p=12684 Many researchers learn flow cytometry in academia, but find new challenges in the commercial sector. In this webinar, learn about regulatory issues and more.

The post Cytometry in Drug Discovery: An Introduction to Regulation, Standardization, and Productivity first appeared on Bio-Radiations.]]> 6 Essential Considerations for Choosing Your Chromatography Resin https://www.bioradiations.com/6-tips-for-the-right-chromatography-resin-1124/ Tue, 12 Nov 2024 18:35:10 +0000 https://www.bioradiations.com/?p=12672 Choosing the right chromatography resin is a complex and critical decision that can significantly impact the success of your downstream purification workflow, whether for preparative or analytical applications. To make an informed choice, you must consider the scientific principles of the process, practical implications for your specific application, and attributes of the manufacturer.

The post 6 Essential Considerations for Choosing Your Chromatography Resin first appeared on Bio-Radiations.]]> What a Single Cell Can Tell You: Exploring Gene Expression and Regulation of Single Cells https://www.bioradiations.com/gene-expression-and-regulation-of-single-cells-1124/ Tue, 12 Nov 2024 18:20:11 +0000 https://www.bioradiations.com/?p=12666 RNA sequencing (RNA-Seq) is a fundamental technique for transcriptome profiling, facilitating the detection of gene expression changes across sample groups. Traditional RNA-Seq of complex tissues, however, is not capable of deciphering the unique biological processes that take place within each cell. As such, single-cell RNA-Seq (scRNA-Seq) has emerged for the analysis of RNA molecules from individual cells, uncovering detailed insights that are unobtainable through bulk RNA-Seq.

The post What a Single Cell Can Tell You: Exploring Gene Expression and Regulation of Single Cells first appeared on Bio-Radiations.]]> Advancing Oncology Research with Immune Response Studies: The Role of xMAP-Based Bio-Plex Multiplex Immunoassays https://www.bioradiations.com/immune-response-studies-in-oncology-research-1124/ Tue, 12 Nov 2024 18:03:10 +0000 https://www.bioradiations.com/?p=12662 In the rapidly evolving field of cancer research, understanding the complex interplay between tumors and the immune system is essential for developing effective therapies. Bio-Plex Multiplex Immunoassays, for research use only and powered by Luminex xMAP technology, provide a cutting-edge tool for simultaneously measuring multiple immune biomarkers. Learn how these research tools are paving the way for development of personalized cancer treatments in the future.

The post Advancing Oncology Research with Immune Response Studies: The Role of xMAP-Based Bio-Plex Multiplex Immunoassays first appeared on Bio-Radiations.]]> 10 Key Factors for Achieving Accurate Cell Counts: Tips, Tricks, and Myths https://www.bioradiations.com/10-key-factors-for-achieving-accurate-cell-counts-tips-tricks-and-myths/ Tue, 12 Nov 2024 17:47:36 +0000 https://www.bioradiations.com/?p=12658 Counting is something we are all familiar with but it’s not always as simple as one, two, three. Measuring the concentration of cells in suspension is a common but surprisingly complex task in cell biology that is easy to get wrong. This article explores ten factors that can influence cell counting, gives advice on how to improve accuracy, and dispels some commonly held myths.

The post 10 Key Factors for Achieving Accurate Cell Counts: Tips, Tricks, and Myths first appeared on Bio-Radiations.]]> Insights from Droplet Digital PCR World 2024 https://www.bioradiations.com/insights-from-droplet-digital-pcr-world-2024-1124/ Tue, 12 Nov 2024 17:40:10 +0000 https://www.bioradiations.com/?p=12655 In June, Bio-Rad hosted Droplet Digital PCR (ddPCR) World 2024, the premier digital PCR event bringing together global leaders in science and innovation. ddPCR World features experts showcasing how ddPCR technology has been instrumental in overcoming some of their most complex research and manufacturing challenges. In this article, we are excited to share key insights and cutting-edge research using ddPCR technology that are driving scientific breakthroughs across the globe.

The post Insights from Droplet Digital PCR World 2024 first appeared on Bio-Radiations.]]> Coming Together Is the Beginning: Working Together Is the Success https://www.bioradiations.com/working-together-is-the-success-1124/ Tue, 12 Nov 2024 17:35:27 +0000 https://www.bioradiations.com/?p=12652 Follow the story of Evitria as it grows from a small Chinese hamster ovary (CHO) cell supernatant supplier to a global therapeutic antibody production company serving clients across the globe. Bio-Rad has partnered with Evitria at every stage of this remarkable journey and looks forward to supporting its continued success.

The post Coming Together Is the Beginning: Working Together Is the Success first appeared on Bio-Radiations.]]> Developing Efficient Downstream Purification Processes for Bispecific Antibodies https://www.bioradiations.com/downstream-purification-of-bispecific-antibodies-1124/ Tue, 12 Nov 2024 17:28:14 +0000 https://www.bioradiations.com/?p=12649 Modern chromatographic resins enable new strategies for downstream processing of bispecific antibodies. Watch this webinar to see case studies and workflows.

The post Developing Efficient Downstream Purification Processes for Bispecific Antibodies first appeared on Bio-Radiations.]]> How to Harness Regulatory Compliance Capabilities with Image Lab Touch Software for Western Blotting and Gel Electrophoresis https://www.bioradiations.com/regulatory-compliance-features-in-image-lab-touch-1124/ Tue, 12 Nov 2024 17:22:50 +0000 https://www.bioradiations.com/?p=12645 Improve western blotting and gel electrophoresis data security with Image Lab Touch Software. See features that support regulatory compliance in this webinar.

The post How to Harness Regulatory Compliance Capabilities with Image Lab Touch Software for Western Blotting and Gel Electrophoresis first appeared on Bio-Radiations.]]> Introducing the ChemiDoc Go Imaging System: Modern Gel and Western Blot Imaging https://www.bioradiations.com/introducing-the-chemidoc-go-imaging-system-824/ Tue, 13 Aug 2024 22:05:03 +0000 https://www.bioradiations.com/?p=12622 A groundbreaking addition to imaging technology has arrived — the ChemiDoc Go Imaging System. The ChemiDoc Go Imaging System is tailored for both gel and western blot imaging, including chemiluminescence and StarBright Blue fluorescent detection. Its compatibility with various trays and LED configurations ensures optimal performance across different experimental setups, including gel excision, nucleic acid staining, and protein detection.

The post Introducing the ChemiDoc Go Imaging System: Modern Gel and Western Blot Imaging first appeared on Bio-Radiations.]]> Enhancing Biotherapeutic Purification Workflows with Mixed-Mode Chromatography https://www.bioradiations.com/biotherapeutic-purification-with-mixed-mode-chromatography-824/ Tue, 13 Aug 2024 21:41:29 +0000 https://www.bioradiations.com/?p=12615 As biotherapeutic modalities have diversified and production processes become more advanced, an evolution in purification strategies is also needed. Modern biotherapeutic purification must be capable of removing multiple types of impurities without compromising yield or quality, in a cost-effective manner. To meet these needs, many are turning to mixed-mode chromatography.

The post Enhancing Biotherapeutic Purification Workflows with Mixed-Mode Chromatography first appeared on Bio-Radiations.]]> Assay Controls and Reference Gene Selection are Essential Elements of Your Real-Time PCR Reactions https://www.bioradiations.com/controls-and-reference-genes-in-real-time-pcr-824/ Tue, 13 Aug 2024 21:26:02 +0000 https://www.bioradiations.com/?p=12613 For a successful real-time PCR experiment, mastering assay controls and reference gene selection are critical. These key steps in your experimental workflow pave the way for reliable data and help you unlock the full potential of real-time PCR.

The post Assay Controls and Reference Gene Selection are Essential Elements of Your Real-Time PCR Reactions first appeared on Bio-Radiations.]]> More to Learn from ESR1 Using ctDNA Analysis https://www.bioradiations.com/more-to-learn-from-esr1-using-ctdna-analysis-824/ Tue, 13 Aug 2024 21:18:36 +0000 https://www.bioradiations.com/?p=12610 Estrogen receptor 1 (ESR1) mutations in breast tumors were first identified nearly 30 years ago. Since then, technology has evolved, as has our understanding of the impact these mutations have on tumor cell physiology. Given the dynamic nature of ESR1 mutation acquisition and enrichment, ctDNA samples appear to be the most robust source for analysis. Droplet Digital PCR (ddPCR) enables rapid, highly sensitive, cost-effective analysis of ctDNA, providing a powerful tool for this critical area of cancer research.

The post More to Learn from ESR1 Using ctDNA Analysis first appeared on Bio-Radiations.]]> Step into the Future of Blot and Gel Imaging with the ChemiDoc Go Imaging System https://www.bioradiations.com/blot-and-gel-imaging-with-chemidoc-go-system-824/ Tue, 13 Aug 2024 20:57:01 +0000 https://www.bioradiations.com/?p=12606 The ChemiDoc Go Imaging System, the newest in the ChemiDoc imaging line, effortlessly captures precise, high-sensitivity images of western blots and nucleic acid gels from a compact benchtop system — transforming lab workflows. Explore how this next-generation imager redefines your research capabilities across various applications, including multiplexing and stain-free protein detection.

The post Step into the Future of Blot and Gel Imaging with the ChemiDoc Go Imaging System first appeared on Bio-Radiations.]]> Faster PCR and Flow Cytometry Highlighted in Annual Lab Automation Roundup https://www.bioradiations.com/faster-pcr-and-flow-cytometry-with-lab-automation-824/ Tue, 13 Aug 2024 19:53:00 +0000 https://www.bioradiations.com/?p=12602 The annual international Society for Laboratory Automation and Screening (SLAS) conference is the highlight of the year for scientists in academia, industry, and government focused on leveraging the power of automation to achieve scientific breakthroughs. Here are some highlights of this year’s conference and how Bio-Rad contributed to it.

The post Faster PCR and Flow Cytometry Highlighted in Annual Lab Automation Roundup first appeared on Bio-Radiations.]]> Strategies to Address Large Molecule Purification Challenges https://www.bioradiations.com/strategies-to-address-large-molecule-purification-challenges-824/ Tue, 13 Aug 2024 19:44:48 +0000 https://www.bioradiations.com/?p=12599 Large molecule purification presents significant challenges due to their considerable size. For instance, Immunoglobulin M (IgM) molecules, ranging from 900 to 1,000 kD, play pivotal roles in mammalian immune responses and find extensive applications in diagnostics and therapeutics. Purification of such molecules is particularly intricate owing to several factors including their weak Protein A binding sites, limited dynamic binding capacity, and slow diffusion rates.

The post Strategies to Address Large Molecule Purification Challenges first appeared on Bio-Radiations.]]> Unlocking the Potential of Cell and Gene Therapies: Overcoming Production Challenges https://www.bioradiations.com/cell-and-gene-therapy-production-challenges-824/ Tue, 13 Aug 2024 19:37:47 +0000 https://www.bioradiations.com/?p=12594 A common challenge in scaling up cell and gene therapy production is monitoring for contaminants, such as Mycoplasma. Learn how ddPCR can help in this webinar.

The post Unlocking the Potential of Cell and Gene Therapies: Overcoming Production Challenges first appeared on Bio-Radiations.]]> Maximize Genomic Insights by Combining Molecular Profiling Methods https://www.bioradiations.com/maximize-insights-by-combining-molecular-profiling-624/ Tue, 11 Jun 2024 19:35:52 +0000 https://www.bioradiations.com/?p=12580 Dive into an in-depth exploration of the molecular methods for gene expression profiling — highlighting the strengths, applications, and uses of quantitative PCR (qPCR), Droplet Digital PCR (ddPCR), and RNA sequencing (RNA-Seq) technologies.

The post Maximize Genomic Insights by Combining Molecular Profiling Methods first appeared on Bio-Radiations.]]> Flow Cytometry and the Law of Bottlenecks https://www.bioradiations.com/flow-cytometry-and-the-law-of-bottlenecks-624/ Tue, 11 Jun 2024 19:13:40 +0000 https://www.bioradiations.com/?p=12576 Manufacturing and high-throughput screening may seem to have very little in common, but they are related at a fundamental level. Both seek to transform a raw material into a finished product, and both struggle with constraints, or bottlenecks, that reduce efficiency. How can we apply the revolutionary 20th century management principles of continuous improvement to 21st century flow cytometry?

The post Flow Cytometry and the Law of Bottlenecks first appeared on Bio-Radiations.]]> Rising from the Ashes: The History of the 488 nm Laser https://www.bioradiations.com/the-history-of-the-488-nm-blue-laser-624/ Tue, 11 Jun 2024 19:04:42 +0000 https://www.bioradiations.com/?p=12566 Despite the pivotal role of the 488 nm (blue) laser in the rise in popularity of flow cytometry, it experienced somewhat of a fall from grace following the shift to higher usage of the 561 nm (yellow) laser. However, with the advancement of flow cytometry leading to the use of more complex multicolor panels and ever-increasing fluorophore numbers per experiment, the blue laser is starting to make a comeback. Discover how StarBright Blue Dyes can help you make the most out of your 488 nm laser.

The post Rising from the Ashes: The History of the 488 nm Laser first appeared on Bio-Radiations.]]> CHT Chromatography Media: 5 Essential Facts https://www.bioradiations.com/cht-chromatography-media-5-essential-facts-624/ Tue, 11 Jun 2024 18:47:14 +0000 https://www.bioradiations.com/?p=12563 For over 30 years, CHT Ceramic Hydroxyapatite Chromatography Media has been a trusted, proven purification solution for many biopharmaceutical manufacturing processes. CHT Media provides exceptional removal of impurities when other chromatography resins fall short.
In this webcast, explore the essential facts and benefits of this chromatography technology.

The post CHT Chromatography Media: 5 Essential Facts first appeared on Bio-Radiations.]]> PrimePCR Assays: Your Path to a Custom PCR Plate https://www.bioradiations.com/primepcr-assays-your-path-to-custom-pcr-plates-624/ Tue, 11 Jun 2024 18:41:24 +0000 https://www.bioradiations.com/?p=12549 Discover the simplicity of crafting your own custom PrimePCR plate with Bio-Rad. Follow along with our intuitive plate wizard, which helps you select your plate type (96- or 384-well) and compatible qPCR instrument for precise assay delivery. Watch this video to see how.

The post PrimePCR Assays: Your Path to a Custom PCR Plate first appeared on Bio-Radiations.]]> Nature’s Lipid Nanoparticles: Challenges and Opportunities in Isolation and Analysis of Extracellular Vesicles https://www.bioradiations.com/extracellular-vesicle-isolation-and-analysis-624/ Tue, 11 Jun 2024 18:39:49 +0000 https://www.bioradiations.com/?p=12556 In nature, mammalian extracellular vesicles (EVs) are vital for intercellular communication, immune responses, and cell removal. EVs also hold promise in diagnostics, therapeutics, and vaccine delivery due to their abundance and natural role as carriers. Explore how overcoming challenges in EV purification and precise RNA analysis methods can help unlock their potential applications.

The post Nature’s Lipid Nanoparticles: Challenges and Opportunities in Isolation and Analysis of Extracellular Vesicles first appeared on Bio-Radiations.]]> Effective Strategies for Endotoxin Removal in Therapeutic Protein Purification https://www.bioradiations.com/endotoxin-removal-in-therapeutic-protein-purification-624/ Tue, 11 Jun 2024 17:57:17 +0000 https://www.bioradiations.com/?p=12552 Endotoxins are common impurities that pose safety risks in therapeutic protein preparations and are difficult to remove. See proven workflows and strategies.

The post Effective Strategies for Endotoxin Removal in Therapeutic Protein Purification first appeared on Bio-Radiations.]]> Guidance for the Validation of qPCR and ddPCR Assays in Regulated Environments https://www.bioradiations.com/qpcr-and-ddpcr-assay-validation-in-regulated-environments-624/ Tue, 11 Jun 2024 17:46:49 +0000 https://www.bioradiations.com/?p=12542 In this webinar, learn about validating qPCR and ddPCR assays in regulated environments. Explore validation parameters and regulatory considerations.

The post Guidance for the Validation of qPCR and ddPCR Assays in Regulated Environments first appeared on Bio-Radiations.]]> A Polishing Strategy for Removing Impurities in Bispecific Antibody Purification https://www.bioradiations.com/a-polishing-strategy-for-removing-impurities-in-bispecific-antibody-purification-224/ Mon, 12 Feb 2024 18:21:19 +0000 https://www.bioradiations.com/?p=12509 Bispecific antibodies pose unique challenges in downstream processing. See how impurities were efficiently removed by mixed-mode chromatography in this webinar.

The post A Polishing Strategy for Removing Impurities in Bispecific Antibody Purification first appeared on Bio-Radiations.]]> Replication-Competent Virus Testing: A Critical Step in Cell and Gene Therapy Quality Control https://www.bioradiations.com/replication-competent-virus-testing-for-qc-224/ Mon, 12 Feb 2024 18:19:18 +0000 https://www.bioradiations.com/?p=12522 Scientists commonly use lentiviruses and adeno-associated viruses (AAVs) as viral vectors in cell and gene therapy (CGT) production. However, therapies that use these viruses must undergo strict quality control measures, including testing for replication-competent viruses (RCV), to ensure that the treatment is safe. Cell culture-based methods are currently the gold standard for RCV testing, but it can take up to 45 days for results to be returned. Droplet Digital PCR (ddPCR) kits now offer an attractive orthogonal method.

The post Replication-Competent Virus Testing: A Critical Step in Cell and Gene Therapy Quality Control first appeared on Bio-Radiations.]]> CAR T-Cell Therapy and the Cytokine Storm: The Balancing Act at the Center of Next-Generation Cancer Therapies https://www.bioradiations.com/car-t-cell-therapy-and-the-cytokine-storm-224/ Mon, 12 Feb 2024 18:18:07 +0000 https://www.bioradiations.com/?p=12530 Recent advances in chimeric antigen receptor (CAR) T-cell therapy have opened exciting new avenues for the treatment of a variety of malignancies. Cytokine release syndrome (CRS), a powerful immune response to activated CAR T cells, is the primary side effect of CAR T-cell therapy, but existing CRS treatments can make CAR T-cell treatment less effective. In two recent studies, researchers used multiplexed cytokine immunoassays to better understand how CAR T-cell therapy triggers CRS and to identify strategies for early intervention in CRS development.

The post CAR T-Cell Therapy and the Cytokine Storm: The Balancing Act at the Center of Next-Generation Cancer Therapies first appeared on Bio-Radiations.]]> NalaGenetics Moves Pharmacogenomics from the Bench to the Clinic using qPCR: An Interview with Astrid Irwanto, Co-Founder and COO https://www.bioradiations.com/moving-pharmacogenomics-from-bench-to-clinic-224/ Mon, 12 Feb 2024 18:14:12 +0000 https://www.bioradiations.com/?p=12516 Astrid Irwanto is the co-founder and COO of NalaGenetics, a company taking a novel, personalized approach to understanding genetic susceptibility to drug reactions in Southeast Asian populations. A PhD in human genetics and postdoctoral research in genetics disease, along with personal experiences, fuel Astrid’s pursuits. In this interview, we discuss NalaGenetics’ approach to pharmacogenomics and why Astrid decided to translate her academic research to a molecular diagnostics company.

The post NalaGenetics Moves Pharmacogenomics from the Bench to the Clinic using qPCR: An Interview with Astrid Irwanto, Co-Founder and COO first appeared on Bio-Radiations.]]> Scalable AAV Vector Purification Workflow Using AEX and Mixed-Mode Chromatography https://www.bioradiations.com/scalable-aav-vector-purification-workflow-224/ Mon, 12 Feb 2024 18:12:35 +0000 https://www.bioradiations.com/?p=12527 Anion exchange and mixed-mode chromatography offer efficient and flexible AAV purification. Learn new strategies for optimizing viral vector purification.

The post Scalable AAV Vector Purification Workflow Using AEX and Mixed-Mode Chromatography first appeared on Bio-Radiations.]]> StarBright Dyes for Flow Cytometry https://www.bioradiations.com/starbright-dyes-for-flow-cytometry-224/ Mon, 12 Feb 2024 18:11:40 +0000 https://www.bioradiations.com/?p=12501 Watch this video to learn about the many benefits of StarBright Dyes — our proprietary fluorescent nanoparticles — and see how they can help you build bigger, better flow cytometry panels with ease. StarBright Dyes have been designed to be stable, with superior brightness, narrow excitation and emission characteristics, and the flexibility to be included in new and existing experiments. They offer improved resolution of specific cell populations and minimized spillover and spreading.

The post StarBright Dyes for Flow Cytometry first appeared on Bio-Radiations.]]> Developing Biotherapeutics for Lung Cancer Treatment https://www.bioradiations.com/biotherapeutics-for-lung-cancer-treatment-224/ Mon, 12 Feb 2024 18:07:52 +0000 https://www.bioradiations.com/?p=12519 In recent years, biologics — drugs derived from living culture systems — have become increasingly significant in the fight against a plethora of conditions. Unfortunately, the efficiency of these drugs comes at a cost. A daily dose of a biologic drug costs, on average, 22 times more than conventional small-molecule drugs. Therefore, biosimilars are developed to make them more accessible. Lung cancer is an example where the development of biosimilars could increase treatment options available for patients.

The post Developing Biotherapeutics for Lung Cancer Treatment first appeared on Bio-Radiations.]]> Enhancing the Power of Microsatellite Instability Detection with ddPCR Technology https://www.bioradiations.com/enhance-the-power-of-msi-detection-with-ddpcr-1123/ Tue, 14 Nov 2023 20:07:21 +0000 https://www.bioradiations.com/?p=12478 As the third most prevalent cancer worldwide, colorectal cancer (CRC) is a significant global health burden. Biomarker identification and detection has proven a powerful strategy for improving CRC diagnosis and informing targeted treatment. Microsatellite instability (MSI) is a key biomarker in CRC, and with Droplet Digital PCR (ddPCR), MSI can be accurately detected using a wider range of sample types.

The post Enhancing the Power of Microsatellite Instability Detection with ddPCR Technology first appeared on Bio-Radiations.]]> 7 Ways Custom PCR Reagents and Plastics Fuel Product Development https://www.bioradiations.com/7-ways-custom-pcr-products-fuel-product-development-1123/ Tue, 14 Nov 2023 20:03:09 +0000 https://www.bioradiations.com/?p=12490 One-size-fits-all solutions cannot address the full range of molecular biology needs in areas such as biotech, biopharma, or molecular diagnostics. When your requirements exceed the existing offerings of reagents and plastics on the market, Bio-Rad can partner with you and provide custom solutions. For a deeper look into these customization opportunities, we spoke with Anna Lai, manager of Bio-Rad's custom reagents program, and Jennifer Placek, who manages the company's custom plastics.

The post 7 Ways Custom PCR Reagents and Plastics Fuel Product Development first appeared on Bio-Radiations.]]> Sequenced — Comic Series https://www.bioradiations.com/sequenced-comic-series/ Tue, 14 Nov 2023 19:59:44 +0000 https://www.bioradiations.com/?p=12237 Join Terence, a motivated undergrad, as he experiences the ups and downs of research using next-generation sequencing, learning valuable tips for successful NGS experiments along the way

The post Sequenced — Comic Series first appeared on Bio-Radiations.]]> Automated Multicolumn Monoclonal Antibody Purification https://www.bioradiations.com/automated-monoclonal-antibody-purification-1123/ Tue, 14 Nov 2023 19:56:23 +0000 https://www.bioradiations.com/?p=12487 The current gold standard in monoclonal antibody purification for most researchers is affinity purification with Protein A or G followed by size exclusion chromatography (SEC). Although robust and highly effective, this workflow does have critical pain points and inefficiencies, leaving researchers to feel that they must oversee every step of their workflow, not leaving their chromatography system unattended for more than a few minutes.

The post Automated Multicolumn Monoclonal Antibody Purification first appeared on Bio-Radiations.]]> Did You Know That Beyond Sensitivity, ddPCR Provides Unrivaled Precision and Accuracy? https://www.bioradiations.com/ddpcr-provides-unrivaled-precision-and-accuracy-1123/ Tue, 14 Nov 2023 19:53:26 +0000 https://www.bioradiations.com/?p=12474 Methods that are highly accurate and precise provide the right answer, consistently. For digital PCR (dPCR), Poisson statistics dictate that the accuracy and precision of results are dependent on the accuracy and precision of sample partitions. This means that to get reliable dPCR results, you need to know your partition volume accurately and your partition volumes need to be consistent. See how our Droplet Digital PCR technology provides both accuracy and precision.

The post Did You Know That Beyond Sensitivity, ddPCR Provides Unrivaled Precision and Accuracy? first appeared on Bio-Radiations.]]> The Pioneer Antibody Discovery Platform: Clinical-Quality Lead Candidates in Industry-Leading Timelines https://www.bioradiations.com/the-pioneer-antibody-discovery-platform-1123/ Tue, 14 Nov 2023 19:51:08 +0000 https://www.bioradiations.com/?p=12472 Biotherapeutic antibody discovery is inherently challenging. Amongst the clinically proven technologies for antibody discovery is phage display — a technique utilized by one of the most recent and innovative solutions on the market: the Pioneer Antibody Discovery Platform.

The post The Pioneer Antibody Discovery Platform: Clinical-Quality Lead Candidates in Industry-Leading Timelines first appeared on Bio-Radiations.]]> Efficacy of a Novel Treatment for Multiple Myeloma Established by Flow Cytometry https://www.bioradiations.com/efficacy-of-a-novel-multiple-myeloma-treatment-1123/ Tue, 14 Nov 2023 19:39:59 +0000 https://www.bioradiations.com/?p=12481 Autologous stem cell transplantation (ASCT) is an effective treatment strategy for multiple myeloma (MM) but isolating enough hematopoietic stem and progenitor cells (HSPCs) for transplantation can be challenging. The selective CXCR4 inhibitor motixafortide has been shown to be a safe and efficacious HSPC mobilization agent in a phase 2 trial when paired with G-CSF. Crees et al. (2023) report the results of the phase 3 trial, in which they used the ZE5 Cell Analyzer to explore motixafortide’s mobilizing effects.

The post Efficacy of a Novel Treatment for Multiple Myeloma Established by Flow Cytometry first appeared on Bio-Radiations.]]> Explore the Epigenomic Landscape of Single Cells with the scATAC You Seq https://www.bioradiations.com/explore-the-epigenomic-landscape-of-single-cells-1123/ Tue, 14 Nov 2023 19:39:16 +0000 https://www.bioradiations.com/?p=12470 Gain access to high-performance chromatin profiling to unravel the complexities of cellular heterogeneity, differentiation, and disease-specific alterations in gene regulation. With single-cell ATAC-seq (assay for transposase-accessible chromatin using sequencing), you can delve into the gene regulatory landscape of individual cells, unlocking invaluable insights into the epigenomic mechanisms that shape their behavior.

The post Explore the Epigenomic Landscape of Single Cells with the scATAC You Seq first appeared on Bio-Radiations.]]> mAb Purification Process Development Using Mixed-Mode Resins — The DOE Approach https://www.bioradiations.com/mab-purification-process-development-1123/ Tue, 14 Nov 2023 19:36:16 +0000 https://www.bioradiations.com/?p=12476 In this case study, process chromatography application scientist Artur Stanczak presents a two-step mAb purification workflow, comparing multimodal resin alternatives used for the polishing step that occurs after protein A capture. The design of experiment (DOE) data presented characterizes the behavior of multiple mAbs with two different mixed-mode resins. The importance of scale, prior knowledge, and the stage of development will also be discussed. The results of both mixed-mode resin studies and the different DOE approaches demonstrate the ability to achieve high-purity products.

The post mAb Purification Process Development Using Mixed-Mode Resins — The DOE Approach first appeared on Bio-Radiations.]]> Revolutionizing Efficacy and Safety Studies by Combining Organ-on-Chip Technology with xMAP-Based Bio-Plex Immunoassays https://www.bioradiations.com/combining-organs-on-chips-with-bio-plex-assays-1123/ Tue, 14 Nov 2023 19:28:02 +0000 https://www.bioradiations.com/?p=12483 Discover how researchers are utilizing xMAP Multiplex Immunoassays to monitor biomarkers associated with tubular nephrotoxicity in kidney proximal tubule chips.

The post Revolutionizing Efficacy and Safety Studies by Combining Organ-on-Chip Technology with xMAP-Based Bio-Plex Immunoassays first appeared on Bio-Radiations.]]> Mastering MIQE: How to Publish High-Quality qPCR Data https://www.bioradiations.com/mastering-miqe-how-to-publish-qpcr-data-1123/ Tue, 14 Nov 2023 19:26:42 +0000 https://www.bioradiations.com/?p=12450 To obtain and publish the best quality qPCR data, you need to follow MIQE guidelines. See how to apply the MIQE guidelines to your experiments in this webinar.

The post Mastering MIQE: How to Publish High-Quality qPCR Data first appeared on Bio-Radiations.]]> Deciphering Cancer Cell Heterogeneity for Precision Medicine https://www.bioradiations.com/deciphering-cancer-cell-heterogeneity-webinar-823/ Tue, 08 Aug 2023 20:39:47 +0000 https://www.bioradiations.com/?p=12440 scATAC-seq is a powerful tool for studying cancer cell heterogeneity. Watch this webinar to see how scATAC-seq can deliver greater insights to your research.

The post Deciphering Cancer Cell Heterogeneity for Precision Medicine first appeared on Bio-Radiations.]]> Fundamentals of Real-Time PCR: The Aftershow https://www.bioradiations.com/fundamentals-of-real-time-pcr-the-aftershow-823/ Tue, 08 Aug 2023 20:26:47 +0000 https://www.bioradiations.com/?p=12428 In this special webinar, the presenters of our Gene Expression University: Fundamentals of Real-Time PCR series answer frequently asked questions. Watch now.

The post Fundamentals of Real-Time PCR: The Aftershow first appeared on Bio-Radiations.]]> Unraveling the Mysteries of Molecular Machines https://www.bioradiations.com/unraveling-the-mysteries-of-molecular-machines-823/ Tue, 08 Aug 2023 19:11:03 +0000 https://www.bioradiations.com/?p=12422 Seychelle M. Vos, PhD, has a passion for science that was ignited at a young age while exploring her parents’ garden and the natural world around her. Now she studies some of the biggest questions in genome biology: how DNA structure and chromatin influence protein transcription. The molecular machines that physically couple transcription and DNA structure by changing the position of nucleosomes, interfacing with chromatin, and compressing DNA into loops, are the focus of Vos’ research.

The post Unraveling the Mysteries of Molecular Machines first appeared on Bio-Radiations.]]> Poly(A) Tails: A Critical Quality Attribute in mRNA-based Therapeutics https://www.bioradiations.com/polya-tails-in-mrna-based-therapeutics-823/ Tue, 08 Aug 2023 18:30:23 +0000 https://www.bioradiations.com/?p=12419 The therapeutic potential of messenger RNA (mRNA) therapeutics has an unprecedented ability to target previously undruggable diseases. The poly(A) tail drives the therapeutic efficacy of the mRNA molecule. Using Droplet Digital PCR (ddPCR) technology to analyze poly(A) tail length and integrity yields unrivaled sensitivity, enabling developers to ensure high poly(A) tail integrity and, ultimately, functional therapeutic mRNAs.

The post Poly(A) Tails: A Critical Quality Attribute in mRNA-based Therapeutics first appeared on Bio-Radiations.]]> PCR Plates: 5 Tips to Guide Your Buying Decision https://www.bioradiations.com/pcr-plates-5-tips-to-guide-your-buying-decision-823/ Tue, 08 Aug 2023 18:13:21 +0000 https://www.bioradiations.com/?p=12408 Learn about 5 key aspects of a PCR plate that are crucial to your results. These tips provide you with the tools to make an informed decision when purchasing PCR plates.

The post PCR Plates: 5 Tips to Guide Your Buying Decision first appeared on Bio-Radiations.]]> Introducing the PTC Tempo Thermal Cycler https://www.bioradiations.com/introducing-the-ptc-tempo-thermal-cycler-823/ Mon, 07 Aug 2023 21:04:11 +0000 https://www.bioradiations.com/?p=12405 The PTC Tempo Thermal Cycler represents the next generation of conventional PCR thermal cyclers from Bio-Rad. Engineered to maintain thermal performance, the PTC Tempo Thermal Cycler offers the flexibility to scale with your laboratory. The design features a refreshed, intuitive user interface and flexible connectivity options for streamlining protocol management. A motorized lid enables automation, taking your PCR to the next level with modern instrument control.

The post Introducing the PTC Tempo Thermal Cycler first appeared on Bio-Radiations.]]> Accurate Titering of Lentiviral Vectors is Paramount for Biotherapeutic Success https://www.bioradiations.com/accurate-titering-of-lentiviral-vectors-823/ Mon, 07 Aug 2023 20:48:37 +0000 https://www.bioradiations.com/?p=12399 Lentiviral vectors (LVVs) have become the primary method of gene delivery for cells ex vivo in both academic and clinical applications. Currently, qPCR is the gold standard for functional titering of LVVs, but Droplet Digital PCR (ddPCR) offers an attractive alternative due to its inherent benefits, such as absolute quantification without the use of a standard curve and enhanced resistance to PCR inhibitors.

The post Accurate Titering of Lentiviral Vectors is Paramount for Biotherapeutic Success first appeared on Bio-Radiations.]]> In-Cell Western: A Versatile Tool for Quantitative Analysis of Protein Expression In-Situ https://www.bioradiations.com/in-cell-western-a-versatile-tool-823/ Mon, 07 Aug 2023 20:38:44 +0000 https://www.bioradiations.com/?p=12396 Protein expression analysis is essential in cell biology research, as it provides valuable insights into the molecular mechanisms that underlie cellular functions. Traditional methods for protein analysis, such as western blotting and ELISA, are useful for studying protein expression in cell lysates. However, these methods require labor-intensive sample preparation and do not provide information on the spatial distribution of proteins within intact cells. In-Cell Western is a powerful technique that enables quantitative and qualitative in situ protein expression analysis.

The post In-Cell Western: A Versatile Tool for Quantitative Analysis of Protein Expression In-Situ first appeared on Bio-Radiations.]]> Efficient and Rapid Purification of E. coli–Expressed Toxin Recombinant Protein Fragments https://www.bioradiations.com/efficient-purification-of-recombinant-toxin-823/ Mon, 07 Aug 2023 20:22:55 +0000 https://www.bioradiations.com/?p=12383 Tetanus toxoid (TT) is a common carrier protein for conjugate vaccines, but its production is faced with significant challenges. Learn about a purification process that yields over 98% pure target protein in this video.

The post Efficient and Rapid Purification of E. coli–Expressed Toxin Recombinant Protein Fragments first appeared on Bio-Radiations.]]> Microphysiological Systems: A Game Changer for Drug Safety and Efficacy Studies? https://www.bioradiations.com/microphysiological-systems-for-drug-safety-studies-523/ Wed, 10 May 2023 08:24:53 +0000 https://www.bioradiations.com/?p=12350 In December 2022, a groundbreaking law was passed granting the U.S. FDA the authority to consider data from new alternative methods in the preclinical testing phase for new drug development. Among the new methods are 3D cell cultures that mimic human tissues and organs, known as microphysiological systems (MPS). The new law aims to improve clinical outcomes while decreasing reliance on animal testing. In this article, we discuss MPS and review data showing how they can synergize with Bio-Plex Assays.

The post Microphysiological Systems: A Game Changer for Drug Safety and Efficacy Studies? first appeared on Bio-Radiations.]]> Next-Generation Chromatography Teaching Labs Set Students Up for Success https://www.bioradiations.com/next-generation-chromatography-teaching-labs-523/ Wed, 10 May 2023 08:21:16 +0000 https://www.bioradiations.com/?p=12360 Dominique Ingato, PhD, is a faculty member at MiraCosta College’s Department of Biotechnology. As one of only fifteen community colleges in California to offer a bachelor’s degree, MiraCosta College’s bachelor’s program in biomanufacturing is breaking ground. As part of this program, Ingato runs a next-generation chromatography (NGC) teaching lab, where students receive practical, hands-on training using state-of-the-art instruments, such as the NGC Chromatography System.

The post Next-Generation Chromatography Teaching Labs Set Students Up for Success first appeared on Bio-Radiations.]]> How to Build Bigger Immunophenotyping Panels with StarBright Dyes https://www.bioradiations.com/build-bigger-panels-with-starbright-dyes-523/ Wed, 10 May 2023 08:20:05 +0000 https://www.bioradiations.com/?p=12344 With the advancement of technology, flow cytometers are now able to detect a much greater range of fluorescent dyes, facilitating the analysis of more extensive and complex multicolor immunophenotyping assays. But this has also introduced more pitfalls to navigate during the design of these experiments. Find out how StarBright Dyes can help circumvent many of these issues, enabling consistent and reliable results.

The post How to Build Bigger Immunophenotyping Panels with StarBright Dyes first appeared on Bio-Radiations.]]> Achieving True Excellence in RNA Therapeutic Development https://www.bioradiations.com/achieving-excellence-in-rna-therapeutics-523/ Wed, 10 May 2023 08:09:48 +0000 https://www.bioradiations.com/?p=12346 The realm of druggable targets has expanded significantly with the advent of mRNA therapeutics. Scientists require exceptional accuracy and confidence to adequately characterize these therapeutics and their anticipated effects. To this end, Droplet Digital PCR (ddPCR) technology has proven invaluable. With unparalleled precision and absolute quantification, Droplet Digital PCR is revolutionizing the field of mRNA therapeutic development and manufacturing.

The post Achieving True Excellence in RNA Therapeutic Development first appeared on Bio-Radiations.]]> Bridging the Gap: Using SEQuoia RNA-Seq Library Prep Kits with New Short-Read Sequencing Platforms https://www.bioradiations.com/bridging-the-sequencing-gap-with-sequoia-kits-523/ Wed, 10 May 2023 08:08:44 +0000 https://www.bioradiations.com/?p=12352 RNA sequencing (RNA-Seq) is a fundamental discovery technique for determining the expression patterns of encoded genes. RNA-Seq has led to innumerable breakthroughs across disciplines; however, a need remains for new sequencing and library preparation technologies to address gaps in current methods. Read how improved access to RNA-Seq would benefit translational research and how a novel RNA library preparation chemistry can synergize with new sequencing platforms.

The post Bridging the Gap: Using SEQuoia RNA-Seq Library Prep Kits with New Short-Read Sequencing Platforms first appeared on Bio-Radiations.]]> Assessing Viral Clearance in Early-Phase Process Development https://www.bioradiations.com/assess-viral-clearance-in-process-development-523/ Wed, 10 May 2023 07:59:07 +0000 https://www.bioradiations.com/?p=12348 Viral clearance studies are part of a multifaceted approach to ensure biopharmaceutical product safety. To prevent costly changes to a manufacturing process, it is important to assess each operation unit for its efficiency early on during downstream process development. See how a design of experiments (DOE) approach was utilized in this case study to investigate the effect of buffer pH and conductivity on the removal of impurities by an anion exchange (AEX) mixed-mode chromatography resin.

The post Assessing Viral Clearance in Early-Phase Process Development first appeared on Bio-Radiations.]]> mAb Purification Process Development Using Mixed-Mode Resins — The DOE Approach https://www.bioradiations.com/mab-purification-process-development-with-doe-523/ Mon, 08 May 2023 18:58:37 +0000 https://www.bioradiations.com/?p=12357 This webinar shows a 2-step mAb purification workflow and compares multimodal options for polishing. See how these methods can achieve high-purity products.

The post mAb Purification Process Development Using Mixed-Mode Resins — The DOE Approach first appeared on Bio-Radiations.]]> A Rapid, Culture-Free Method to Determine 50% Neutralization Titer (NT50) Using the ACE2-Competitive Bio-Plex Pro SARS-CoV-2 Neutralization Antibody Assay https://www.bioradiations.com/a-rapid-culture-free-method-to-measure-nt50-523/ Mon, 08 May 2023 18:50:16 +0000 https://www.bioradiations.com/?p=12354 In this webinar, see how a Bio-Plex Pro Assay could provide a high-throughput culture-free NT50 determination method for current and future SARS-CoV-2 variants.

The post A Rapid, Culture-Free Method to Determine 50% Neutralization Titer (NT50) Using the ACE2-Competitive Bio-Plex Pro SARS-CoV-2 Neutralization Antibody Assay first appeared on Bio-Radiations.]]> Genotype Screening in Songbirds Made Easy https://www.bioradiations.com/genotype-screening-in-songbirds-made-easy-223/ Tue, 14 Feb 2023 23:01:20 +0000 https://www.bioradiations.com/?p=12299 Christien Bowman, MSc, has traversed a path from psychology to neuroscience, and is now working on attaining his PhD in biomedicine. In this interview, we discussed how Bowman uses Chelex 100 Resin as part of a fast and cost-effective method to determine the sex of zebra finch songbirds. This work ultimately helps Bowman study a behavioral paradigm that creates autism-like features in songbirds.

The post Genotype Screening in Songbirds Made Easy first appeared on Bio-Radiations.]]> 6 Key Considerations for Getting the Long and Short of RNA-Seq with FFPE Samples https://www.bioradiations.com/6-key-considerations-for-ffpe-sample-rna-seq-223/ Tue, 14 Feb 2023 22:32:57 +0000 https://www.bioradiations.com/?p=12291 When it comes to tumor profiling, the poor quality and degradation of RNA in formalin-fixed paraffin-embedded (FFPE) samples are major limitations. To overcome this challenge, we tested a novel RNA-Seq workflow using the SEQuoia Complete Library Prep Kit and the SEQuoia Ribodepletion Kit. This workflow offers more complex transcriptome profiling, including both long and short RNA biotypes, to better represent the complete transcriptome.

The post 6 Key Considerations for Getting the Long and Short of RNA-Seq with FFPE Samples first appeared on Bio-Radiations.]]> Flow Cytometry Empowers CAR T-Cell Therapies https://www.bioradiations.com/flow-cytometry-empowers-car-t-cell-therapies-223/ Tue, 14 Feb 2023 21:26:07 +0000 https://www.bioradiations.com/?p=12288 Chimeric antigen receptor (CAR) T cells represent the next generation of therapeutic interventions and are a major advancement in personalized disease treatment. CAR T-cell therapies utilize a patient’s own T cells to recognize and destroy cancer cells or other disease-causing cells. Find out how the ZE5 Cell Analyzer has helped overcome some of the challenges associated with development of these powerful new therapies.

The post Flow Cytometry Empowers CAR T-Cell Therapies first appeared on Bio-Radiations.]]> Scalable Purification Strategies for Viral Vectors and Vaccines https://www.bioradiations.com/scalable-purification-for-viral-vectors-and-vaccines-223/ Tue, 14 Feb 2023 21:05:00 +0000 https://www.bioradiations.com/?p=12284 Large-scale downstream processing of viruses can face a range of obstacles different from those of many biotherapeutics. These challenges mostly arise from the size and complexity of the virus, which can affect product purity and recovery. Improvements of purification tools are necessary to overcome these challenges and must be engineered for easy scalability to meet manufacturing demands. In this video, Dr. Mark Snyder discusses common difficulties when developing purification strategies for vaccine production and gene therapy.

The post Scalable Purification Strategies for Viral Vectors and Vaccines first appeared on Bio-Radiations.]]> Maximizing Metastasis Insights with a Novel Integrated Workflow for CTC Enrichment and Enumeration https://www.bioradiations.com/metastasis-insights-with-ctc-enrichment-and-enumeration-223/ Tue, 14 Feb 2023 20:40:23 +0000 https://www.bioradiations.com/?p=12275 Watch this webinar to learn how a novel workflow can efficiently isolate CTCs for diverse downstream applications, without disrupting their native biology.

The post Maximizing Metastasis Insights with a Novel Integrated Workflow for CTC Enrichment and Enumeration first appeared on Bio-Radiations.]]> Generating Anti-CD19 CAR T Cells with High Efficiency and Viability via mRNA Electroporation https://www.bioradiations.com/generating-car-t-cells-via-mrna-electroporation-223/ Tue, 14 Feb 2023 20:29:32 +0000 https://www.bioradiations.com/?p=12271 In this webinar, we describe an mRNA electroporation–based workflow for CAR T cell generation. See how high viability and transfection efficiency were achieved.

The post Generating Anti-CD19 CAR T Cells with High Efficiency and Viability via mRNA Electroporation first appeared on Bio-Radiations.]]> Downstream Process Development for the Purification of Biosimilar Insulin from Escherichia coli https://www.bioradiations.com/purification-of-biosimilar-insulin-from-e-coli-223/ Tue, 14 Feb 2023 20:11:32 +0000 https://www.bioradiations.com/?p=12264 This webinar presents a downstream processing workflow for recombinant human insulin production from E. coli. Learn how proinsulin and insulin can be purified.

The post Downstream Process Development for the Purification of Biosimilar Insulin from Escherichia coli first appeared on Bio-Radiations.]]> Quick Tips for Western Blotting https://www.bioradiations.com/quick-tips-for-western-blotting-122/ Tue, 14 Feb 2023 19:30:23 +0000 https://www.bioradiations.com/?p=12039 Learn how to improve your western blotting results with our Quick Tips video series. Watch these 1–2 minute videos see how to perfect each step of the western blotting process.

The post Quick Tips for Western Blotting first appeared on Bio-Radiations.]]> Exploring Circulating Tumor DNA (ctDNA) as a New Oncology Clinical Trial Endpoint https://www.bioradiations.com/exploring-ctdna-as-an-oncology-trial-endpoint-223/ Tue, 14 Feb 2023 18:52:58 +0000 https://www.bioradiations.com/?p=12281 Historically, five-year survival rates have been used to evaluate cancer therapeutic effectiveness in clinical trials. But time is critical for research — and for patients. Fortunately, there is a promising method for evaluating cancer therapeutic effectiveness that could potentially offer prognostic insights after just one year. Noninvasive liquid biopsy analysis, including analysis of circulating tumor DNA, is improving researchers’ ability to detect clinical responses more rapidly.

The post Exploring Circulating Tumor DNA (ctDNA) as a New Oncology Clinical Trial Endpoint first appeared on Bio-Radiations.]]> Solving Protein Purification Challenges https://www.bioradiations.com/solving-protein-purification-challenges-1022/ Tue, 11 Oct 2022 19:06:34 +0000 https://www.bioradiations.com/?p=12225 Carter Mitchell, PhD, is a protein problem solver. As Chief Science Officer at Kemp Proteins, he helps academic and industry researchers develop a range of protein therapeutics, diagnostics, and molecular tools in milligram to gram quantities with bioprocess scale in mind. In this interview, we discussed how Kemp Proteins ensures delivery of high-quality processes and protein-based products to their clients, even when working with difficult proteins.

The post Solving Protein Purification Challenges first appeared on Bio-Radiations.]]> Measure Neutralizing Antibodies against Any SARS-CoV-2 Variant in 1 Day https://www.bioradiations.com/measure-sars-cov-2-variant-neutralizing-antibodies-1022/ Tue, 11 Oct 2022 19:05:50 +0000 https://www.bioradiations.com/?p=12211 Current vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have reduced the severity of the coronavirus disease 2019 (COVID-19). However, the drastic and quick evolution of the virus poses challenges to the development of durable and efficacious therapeutics for vaccine and antibody developers alike. See how the Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit speeds up the qualitative measurement of neutralizing antibodies (NAbs) against new SARS-CoV-2 variants, removing a roadblock in COVID-19 therapeutic development.

The post Measure Neutralizing Antibodies against Any SARS-CoV-2 Variant in 1 Day first appeared on Bio-Radiations.]]> A Pioneering Approach to Biotherapeutic Antibody Discovery https://www.bioradiations.com/pioneering-biotherapeutic-antibody-discovery-1022/ Tue, 11 Oct 2022 19:03:11 +0000 https://www.bioradiations.com/?p=12244 Immunotherapy has revolutionized cancer treatment, yet the drug discovery process to develop new biotherapeutic antibodies remains arduous. However, significant advances in antibody technology such as phage display have enabled fully human antibodies to be developed from animal-free libraries for just about any antigen.

The post A Pioneering Approach to Biotherapeutic Antibody Discovery first appeared on Bio-Radiations.]]> Maximizing the Impact of Wastewater-Based Epidemiology with ddPCR Technology https://www.bioradiations.com/maximizing-the-impact-of-wastewater-based-epidemiology-with-ddpcr-1022/ Tue, 11 Oct 2022 18:48:47 +0000 https://www.bioradiations.com/?p=12198 As SARS-CoV-2 becomes an endemic agent worldwide, communities and health agencies must figure out how to identify novel variants and track infection rates in ways that are sustainable, sensitive, and accurate. Fortunately, wastewater-based epidemiology (WBE) has proven its ability to play an important role in monitoring COVID-19 outbreaks, and Droplet Digital PCR (ddPCR) has become a cornerstone method for ongoing wastewater-based disease surveillance. In this article, we review the role of wastewater-based testing in community health before, during, and beyond the COVID-19 pandemic, and we explore why ddPCR technology is ideally suited for this application as it continues to expand and evolve.

The post Maximizing the Impact of Wastewater-Based Epidemiology with ddPCR Technology first appeared on Bio-Radiations.]]> Using ddPCR Technology to Improve Cancer Detection and Treatment Efficacy Monitoring https://www.bioradiations.com/using-ddpcr-to-improve-cancer-detection-1022/ Tue, 11 Oct 2022 18:35:03 +0000 https://www.bioradiations.com/?p=12232 Cancer remains a leading cause of death worldwide. However, as cancer treatments continue to improve and diagnoses happen sooner, more and more patients are experiencing remission. To further increase survival rates and reduce unnecessary treatment, scientists and clinicians are turning to ultra-sensitive molecular analysis to inform treatment decisions and predict and detect relapse.

The post Using ddPCR Technology to Improve Cancer Detection and Treatment Efficacy Monitoring first appeared on Bio-Radiations.]]> What You Need to Know About Packing Resins at Process Scale https://www.bioradiations.com/packing-resins-at-process-scale-1022/ Tue, 11 Oct 2022 18:33:11 +0000 https://www.bioradiations.com/?p=12208 Learn best practices and guidelines for successfully packing different types of chromatography resins in this video webinar. This includes incompressible resins, like CHT Ceramic Hydroxyapatite Media, as well as traditional compressible resins. In general, parameters such as compression factor, settling rate, and attributes of the base matrix need to be considered when packing resins at process scale. Packing methods can be adapted to obtain simple, highly reproducible packing. Get tips here.

The post What You Need to Know About Packing Resins at Process Scale first appeared on Bio-Radiations.]]> Quick Tips for Bio-Plex Assays https://www.bioradiations.com/quick-tips-for-bio-plex-assays-1022/ Tue, 11 Oct 2022 18:25:03 +0000 https://www.bioradiations.com/?p=12202 It is imperative to plan and prepare ahead of time for your research using Bio-Plex Assays to be successful. Good assay planning and preparation reduces mistakes and makes for a stress-free assay run. In this series of short videos, we share some simple steps you can take to make sure your work goes smoothly.

The post Quick Tips for Bio-Plex Assays first appeared on Bio-Radiations.]]> Learn to Develop a New Multiplex SARS-CoV-2 Variant Neutralizing Antibody Assay in a Single Day https://www.bioradiations.com/develop-a-sars-cov-2-variant-neutralizing-antibody-assay-1022/ Tue, 11 Oct 2022 18:23:36 +0000 https://www.bioradiations.com/?p=12220 Develop new assays in just one day with the Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit. See how in this webinar.

The post Learn to Develop a New Multiplex SARS-CoV-2 Variant Neutralizing Antibody Assay in a Single Day first appeared on Bio-Radiations.]]> Purification of DNA Oligonucleotides using Anion Exchange Chromatography https://www.bioradiations.com/dna-oligonucleotide-purification-with-aex-1022/ Tue, 11 Oct 2022 18:21:40 +0000 https://www.bioradiations.com/?p=12216 In this webcast, see how Nuvia HP-Q Resin, an anion exchange resin, was used to purify oligonucleotides with phosphodiester backbones in a scaleable workflow.

The post Purification of DNA Oligonucleotides using Anion Exchange Chromatography first appeared on Bio-Radiations.]]> Connected Instruments in the Digital Laboratory https://www.bioradiations.com/connected-instruments-in-the-digital-laboratory-722/ Wed, 13 Jul 2022 04:50:00 +0000 https://www.bioradiations.com/?p=12178 As technology advances, a digital transformation has begun in laboratories around the world. Driven by the need to improve data quality and increase the value derived from the investment in generating those data, data management systems, devices, and their workflows are becoming more connected and integrated, ushering in the age of the digital laboratory. The digital laboratory is one where paper lab notebooks are replaced with fully digital data, complete with rich metadata, and generated using integrated workflows. Such data are easy to find, reuse, and reproduce. Further digitization and workflow integration to operate laboratory instruments will advance the burgeoning digital laboratory.

The post Connected Instruments in the Digital Laboratory first appeared on Bio-Radiations.]]> Single-Step Influenza Virus Purification Using an Anion Exchange Resin https://www.bioradiations.com/single-step-influenza-virus-purification-using-an-anion-exchange-resin-722/ Wed, 13 Jul 2022 04:17:06 +0000 https://www.bioradiations.com/?p=12172 A new and simplified anion exchange chromatographic process for the purification of both live and inactivated cell grown H1N1 influenza viruses was established with a recently developed anion exchanger. The resin is designed with an optimized surface extender and pore size for superior accessibility and large biomolecule binding capacity, aiming to overcome common drawbacks of existing ion exchangers for virus purification.

The post Single-Step Influenza Virus Purification Using an Anion Exchange Resin first appeared on Bio-Radiations.]]> Leading the Way in Our Understanding of COVID-19: Peptide Megapool Assays https://www.bioradiations.com/leading-the-way-in-our-understanding-of-covid-19-peptide-megapool-assays-722/ Wed, 13 Jul 2022 03:50:59 +0000 https://www.bioradiations.com/?p=12167 Understanding how T cells respond to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical in developing long-term therapeutic approaches, as well as helping inform strategies for future pandemics. Learn how one team at the La Jolla Institute for Immunology in San Diego, California, has been using novel T-cell activation assays to understand how our immune system responds to SARS-CoV-2.

The post Leading the Way in Our Understanding of COVID-19: Peptide Megapool Assays first appeared on Bio-Radiations.]]> Across the Blot: A Western Blotting Crossword https://www.bioradiations.com/across-the-blot-a-western-blotting-crossword-722/ Wed, 13 Jul 2022 03:19:37 +0000 https://www.bioradiations.com/?p=12164 Do you think you know western blotting? Test your knowledge with a crossword puzzle.

The post Across the Blot: A Western Blotting Crossword first appeared on Bio-Radiations.]]> Precise Cell and Gene Therapy Potency Quantification https://www.bioradiations.com/precise-cell-and-gene-therapy-potency-quantification-722/ Wed, 13 Jul 2022 02:56:38 +0000 https://www.bioradiations.com/?p=12161 Inadequate quality control assays shouldn't limit cell and gene therapy applications. Instead, quantifying the potency of cell and gene therapies is essential for successful therapeutics. In this article, we describe multiple leading potency assays developed using Droplet Digital PCR (ddPCR) technology, including an assay that quantifies transgene integration and expression in one step.

The post Precise Cell and Gene Therapy Potency Quantification first appeared on Bio-Radiations.]]> The CFX Duet Real-Time PCR System: Our Most Accessible qPCR Technology https://www.bioradiations.com/the-cfx-duet-real-time-pcr-system-our-most-accessible-qpcr-technology-722/ Wed, 13 Jul 2022 02:43:34 +0000 https://www.bioradiations.com/?p=12158 Through the CFX Duet Real-Time PCR System, our newest generation of quantitative PCR (qPCR) instrumentation, this technology is now even more accessible for molecular biologists and other professionals doing essential qPCR. With CFX Maestro Software, you can perform duplex detection, gene expression, and genotyping, all without paying for additional optics you don’t need. Check out our video tour of the new CFX Duet System.

The post The CFX Duet Real-Time PCR System: Our Most Accessible qPCR Technology first appeared on Bio-Radiations.]]> Modernizing Gene Editing Detection with Droplet Digital PCR https://www.bioradiations.com/modernizing-gene-editing-detection-with-droplet-digital-pcr-722/ Wed, 13 Jul 2022 02:30:52 +0000 https://www.bioradiations.com/?p=12156 Gene editing is only useful if it’s successful. Read how scientists are leveraging the unparalleled accuracy and specificity of ddPCR technology to optimize quality control in gene editing projects, including HIV detection and CAR-T cell generation.

The post Modernizing Gene Editing Detection with Droplet Digital PCR first appeared on Bio-Radiations.]]> Take the Expressway to RNA Library Prep https://www.bioradiations.com/take-the-expressway-to-rna-library-prep-722/ Wed, 13 Jul 2022 02:18:43 +0000 https://www.bioradiations.com/?p=12154 RNA-Seq library preparation is a manually intensive, time-consuming process prone to bias from the multiple enzymes required. So, how can you trust your sequencing results? Bio-Rad is revolutionizing RNA-Seq library prep with the SEQuoia Express Stranded RNA Library Prep Kit – a three-hour, three-tube solution eliminating the need for multiple enzymes and reducing both the number of pipetting steps and protocol time. The key is an engineered, proprietary retrotransposon called SEQzyme. With this serial template jumping, or continuous synthesis, reaction you can obtain an unbiased, stranded RNA-Seq library constructed to accurately represent both messenger and non-coding RNA from your sample.

The post Take the Expressway to RNA Library Prep first appeared on Bio-Radiations.]]> Multiplex SARS-CoV-2 Variant Neutralization Antibody Assays: Get Multiple Variant Results Fast https://www.bioradiations.com/multiplex-sars-cov-2-variant-neutralization-antibody-assays-get-multiple-variant-results-fast-722/ Wed, 13 Jul 2022 02:10:38 +0000 https://www.bioradiations.com/?p=12151 Bio-Plex Pro SARS-CoV-2 Neutralization Antibody Assays provide reliably measure neutralizing antibodies in less than 3 hours. Watch our webinar to learn more.

The post Multiplex SARS-CoV-2 Variant Neutralization Antibody Assays: Get Multiple Variant Results Fast first appeared on Bio-Radiations.]]> A Mixed-Mode Approach — An Alternative to a Three-Step mAb Purification Process https://www.bioradiations.com/a-mixed-mode-approach-an-alternative-to-a-three-step-mab-purification-process-722/ Wed, 13 Jul 2022 01:41:32 +0000 https://www.bioradiations.com/?p=12148 Monoclonal antibody (mAb) purification methods often involve the use of resin-based chromatography. In this video, we compare a three-step mAb purification workflow, which uses Nuvia Ion Exchange Resins post–Protein A capture, to a two-step purification workflow, which includes a Protein A capture step followed by a mixed-mode chromatography step using Nuvia aPrime 4A Resin, a hydrophobic anion exchange resin. We present an additional two-step purification approach using Protein A resin followed by a calcium affinity cation exchange media, CHT Ceramic Hydroxyapatite XT Media. The results of both studies demonstrate that a high purity product can be attained using one fewer unit operation than traditional workflows.

The post A Mixed-Mode Approach — An Alternative to a Three-Step mAb Purification Process first appeared on Bio-Radiations.]]> High Performance with GMP Ready CHT Prepacked Process-Scale Columns https://www.bioradiations.com/high-performance-with-gmp-ready-cht-prepacked-process-scale-columns-722/ Wed, 13 Jul 2022 01:08:04 +0000 https://www.bioradiations.com/?p=12143 Foresight Pro CHT Prepacked Columns are GMP ready for process-scale purification. Learn about the design and see performance data in this webinar.

The post High Performance with GMP Ready CHT Prepacked Process-Scale Columns first appeared on Bio-Radiations.]]> Generation of Recombinant Tool Antibodies to Support Cell and Gene Therapy Development https://www.bioradiations.com/generation-of-recombinant-tool-antibodies-to-support-cell-and-gene-therapy-development-722/ Wed, 13 Jul 2022 00:54:32 +0000 https://www.bioradiations.com/?p=12139 Recombinant antibodies, CAR-T Cell Therapy Development, CAR-T Cell Therapy, Monoclonal Antibody Generation, custom antibodies, cell and gene therapy development, bioanalysis, biosimilar, immunogenicity, drug monitoring, antibody drug conjugate.

The post Generation of Recombinant Tool Antibodies to Support Cell and Gene Therapy Development first appeared on Bio-Radiations.]]> Top 8 Advantages of Electroporation in Cell and Gene Therapy https://www.bioradiations.com/top-8-advantages-of-electroporation-in-cell-and-gene-therapy-422/ Tue, 12 Apr 2022 16:32:34 +0000 https://www.bioradiations.com/?p=12102 Cell and gene therapies have seen record growth due to their potential to address the underlying causes of genetic and acquired diseases. Non-viral delivery methods are increasing in popularity due to their safety, cost-effectiveness, reduction in manufacturing time, and flexibility compared to viral vector delivery methods. Electroporation is a simple, broadly applicable method capable of delivering molecules to a wide range of cell types for either cell or gene therapy. This article outlines the top advantages of using electroporation in cell and gene therapy and why it might be a good approach for your own research group.

The post Top 8 Advantages of Electroporation in Cell and Gene Therapy first appeared on Bio-Radiations.]]> Unlocking the Power of Analysis with Automation https://www.bioradiations.com/unlocking-the-power-of-analysis-with-automation-422/ Tue, 12 Apr 2022 16:32:00 +0000 https://www.bioradiations.com/?p=12070 Disparate systems, distributed teams, and overly complex technologies are a recipe for inefficiency in research and development (R&D) laboratories, especially in drug development. With the pressure to meet challenging deadlines and strict regulations, it is easy for working practices to become disconnected. To improve the efficiency, accuracy, and consistency of data generation, acquisition, and management, R&D organizations are looking to streamline their lab practices by investing in technologies that automate data generation, accelerate data collection speed and storage, and enhance the quality and reliability of the information produced. Lab automation presents exciting opportunities for future research, promising novel advances in machine learning and artificial intelligence (AI), liquid handling robotics, and cloud-based data workflows.

The post Unlocking the Power of Analysis with Automation first appeared on Bio-Radiations.]]> Evaluating and Understanding the True Spread of COVID-19 through Seroprevalence https://www.bioradiations.com/understanding-covid-19-through-seroprevalence-422/ Tue, 12 Apr 2022 16:31:23 +0000 https://www.bioradiations.com/?p=12085 Circulation of SARS-CoV-2 in the global population led to the emergence of new variants threatening to undermine vaccine effectiveness. Understanding the immune response and its neutralizing abilities at both the patient level and globally could not only help prevent outbreaks but also mitigate the outcome for future pandemics.

The post Evaluating and Understanding the True Spread of COVID-19 through Seroprevalence first appeared on Bio-Radiations.]]> Trends in Wastewater-Based Epidemiology for 2022 https://www.bioradiations.com/trends-in-wastewater-based-epidemiology-for-2022-422/ Tue, 12 Apr 2022 16:29:04 +0000 https://www.bioradiations.com/?p=12108 Two years into the COVID-19 pandemic, trends point to a transition for the SARS-CoV-2 virus into an endemic agent. As major surveillance infrastructure scales back, it will be critical to maintain a cost-effective, simple, and rapid strategy for detecting and monitoring the spread of new variants. The key to such a strategy? Wastewater, where all disease agents leave their unique signature.

The post Trends in Wastewater-Based Epidemiology for 2022 first appeared on Bio-Radiations.]]> Powering Antibody Production and Purification with the NGC Chromatography System https://www.bioradiations.com/antibody-production-with-the-ngc-system-422/ Tue, 12 Apr 2022 16:22:05 +0000 https://www.bioradiations.com/?p=12068 For this article, we spoke with Vera Dreyfuss of evitria AG, an antibody expression services provider based in Switzerland, about the role that the NGC Chromatography System has played in powering the growth and success of the company.

The post Powering Antibody Production and Purification with the NGC Chromatography System first appeared on Bio-Radiations.]]> The PI3K-AKT Pathway and Its Role in Tumorigenesis https://www.bioradiations.com/the-pi3k-akt-pathway-and-its-role-in-tumorigenesis-422/ Tue, 12 Apr 2022 16:21:43 +0000 https://www.bioradiations.com/?p=12076 The phosphoinositide 3-kinase/protein kinase B (PI3K-AKT) pathway is involved in the control of numerous critical cellular processes. PI3K-AKT pathway dysfunction has been observed in several diseases, including cancer, cardiovascular disease, and autoimmune and neurological disorders. In this animation, learn more about the molecular characteristics and function of the PI3K-AKT pathway and how AKT mutation plays a role in tumorigenesis.

The post The PI3K-AKT Pathway and Its Role in Tumorigenesis first appeared on Bio-Radiations.]]> Wastewater SARS-CoV-2 Testing with Droplet Digital PCR (ddPCR) Technology https://www.bioradiations.com/wastewater-sars-cov-2-testing-with-ddpcr-422/ Tue, 12 Apr 2022 16:20:44 +0000 https://www.bioradiations.com/?p=12111 Wastewater-based epidemiology indirectly monitors infection rates in a community based on pathogen levels in local wastewater. Because symptomatic and asymptomatic SARS-CoV-2 infected individuals shed viral RNA in sewer systems, analysis of SARS-CoV-2 in sewage networks can provide information about SARS-CoV-2 infections within the community. However, the accuracy of these data depends on the quality of the wastewater testing method. During the COVID-19 pandemic, Droplet Digital PCR has proven that it has the sensitivity needed to detect outbreaks and monitor SARS-CoV-2 variants in communities via wastewater testing.

The post Wastewater SARS-CoV-2 Testing with Droplet Digital PCR (ddPCR) Technology first appeared on Bio-Radiations.]]> Manufacturing a Recombinant Retrovirus — Downstream Purification Process Development https://www.bioradiations.com/manufacturing-a-recombinant-retrovirus-422/ Tue, 12 Apr 2022 16:19:30 +0000 https://www.bioradiations.com/?p=12079 Enveloped virus and VLP purification can be difficult. Learn about key factors and a cGMP-compatible, low cost, scalable process for retrovirus vector capture.

The post Manufacturing a Recombinant Retrovirus — Downstream Purification Process Development first appeared on Bio-Radiations.]]> The ZE5 Cell Analyzer, a Fast and Flexible Flow Cytometer for High-Throughput Screening https://www.bioradiations.com/the-fast-and-flexible-ze5-cell-analyzer-422/ Tue, 12 Apr 2022 16:18:41 +0000 https://www.bioradiations.com/?p=12082 High-throughput screening (HTS) is a critical component of the entire drug discovery process. Flow cytometry is an attractive alternative to imaging and plate reader assays for HTS in drug discovery, due to its increased sensitivity and multiplexing capabilities. The ZE5 Cell Analyzer eliminates pain points that have hindered the full adoption of flow cytometry for drug discovery.

The post The ZE5 Cell Analyzer, a Fast and Flexible Flow Cytometer for High-Throughput Screening first appeared on Bio-Radiations.]]> Scientists for Life: A Tale of Two Tattoos https://www.bioradiations.com/scientists-for-life-a-tale-of-two-tattoos-122/ Tue, 11 Jan 2022 08:59:46 +0000 https://www.bioradiations.com/?p=11991 Most scientists, no matter where they are at in their career, remember the moments of wonder as they navigated lab work for the first time. Whether it’s a band on a gel, a colony on a plate, or a piece of data, those first tangible results come with a feeling of having accomplished something incredible. More than ten years later, we caught up with two scientists who immortalized that feeling in the form of matching protein ladder tattoos, and discussed how their time as undergraduate researchers led them where they are today.

The post Scientists for Life: A Tale of Two Tattoos first appeared on Bio-Radiations.]]> Bispecific Antibodies and Cancer Immunotherapy https://www.bioradiations.com/bispecific-antibodies-and-cancer-immunotherapy-122/ Tue, 11 Jan 2022 08:59:13 +0000 https://www.bioradiations.com/?p=12024 Bispecific antibodies (bsAbs) are an important addition to the immuno-oncology toolbox. Designed to recognize two distinct epitopes, bsAbs have enhanced binding, specificity, and efficacy compared to current monovalent antibody therapeutics, making them exciting candidates for more targeted cancer treatments. Learn about bsAbs and how our tools can help scientists with their research on therapeutic candidates.

The post Bispecific Antibodies and Cancer Immunotherapy first appeared on Bio-Radiations.]]> Monitoring CAR T-Cell Therapy Quality with Droplet Digital PCR https://www.bioradiations.com/monitoring-car-t-cell-therapy-quality-with-droplet-digital-pcr-122/ Tue, 11 Jan 2022 08:58:38 +0000 https://www.bioradiations.com/?p=12028 The CAR T-cell manufacturing process involves high potential for variability. Learn how sensitive ddPCR assays can be used to ensure safety and efficacy.

The post Monitoring CAR T-Cell Therapy Quality with Droplet Digital PCR first appeared on Bio-Radiations.]]> 5 Reasons to Read the Antibody Advice Guide https://www.bioradiations.com/5-reasons-to-read-the-antibody-advice-guide-122/ Tue, 11 Jan 2022 08:57:55 +0000 https://www.bioradiations.com/?p=12034 In the day-to-day pressures of a research environment, it is natural to want to generate important data as quickly as possible. However, there are real benefits in ensuring that the antibody you have picked is the best for your experiment and spending time aliquoting and titrating your antibodies. Bio-Rad recently launched a free Antibody Advice Guide to equip scientists with the knowledge to get the best out of their experiments with antibodies. Read on for five examples of valuable information that you will learn from the guide, helping you generate publication-ready data.

The post 5 Reasons to Read the Antibody Advice Guide first appeared on Bio-Radiations.]]> Viral RNA Preparation for Molecular Pathogen Detection: A New Application for Chelex 100 Resin and InstaGene Matrix https://www.bioradiations.com/viral-rna-preparation-for-molecular-pathogen-detection-a-new-application-for-chelex-100-resin-and-instagene-matrix-122/ Tue, 11 Jan 2022 08:56:31 +0000 https://www.bioradiations.com/?p=12021 Although vaccines have helped alleviate the negative impact of COVID-19, its persistence across many areas of the globe reveals a continuous need for rapid, accurate, and economical COVID-19 testing. But is it even possible to isolate and detect viral RNA rapidly and economically? Here we highlight a repurposed tool for improved COVID-19 detection: Chelex 100 Resin and the related InstaGene Matrix for rapid, cost-effective viral RNA preservation and detection.

The post Viral RNA Preparation for Molecular Pathogen Detection: A New Application for Chelex 100 Resin and InstaGene Matrix first appeared on Bio-Radiations.]]> The Process of Resin Screening https://www.bioradiations.com/the-process-of-resin-screening-122/ Tue, 11 Jan 2022 08:55:48 +0000 https://www.bioradiations.com/?p=12059 Process development scientists, faced with an increasing number of proteins or complex biomolecules, continuously search for robust purification methods that can be rapidly scaled for manufacturing. What are the optimal purification conditions for high throughput and yield? Which resin or resin combination best purifies the desired protein? How can an efficient, yet cost-effective process be established? The answer: use a systematic screening approach. Read on to learn more.

The post The Process of Resin Screening first appeared on Bio-Radiations.]]> Neutralizing Antibody Assays for SARS-CoV-2 Variant Studies https://www.bioradiations.com/neutralizing-antibody-assays-for-sars-cov-2-variant-studies-122/ Tue, 11 Jan 2022 08:41:37 +0000 https://www.bioradiations.com/?p=12016 With COVID-19 vaccines now widely available, case numbers in many areas are falling. But the question remains: How long does vaccine-induced immunity last? Measuring isotype-specific antibodies against SARS-CoV-2 and the effectiveness of neutralizing antibodies against variants can help us better understand the duration and breadth of the humoral response. These data are critical to aid public health researchers and inform development decisions for vaccines and therapeutics. Read about the new Bio-Plex Pro Human SARS-CoV-2 Variant Neutralization Antibody 11-Plex Panel.

The post Neutralizing Antibody Assays for SARS-CoV-2 Variant Studies first appeared on Bio-Radiations.]]> A Novel Technology for Post-Library rRNA Depletion https://www.bioradiations.com/a-novel-technology-for-post-library-rrna-depletion-122/ Tue, 11 Jan 2022 08:39:46 +0000 https://www.bioradiations.com/?p=12047 The SEQuoia RiboDepletion Kit employs novel technology to deplete ribosomal RNA while preserving rare transcripts and small RNAs, as well as minimizing the risk of degrading or low abundant samples. The SEQuoia RiboDepletion Kit also offers a streamlined workflow and multiplex compatibility, leading to significant time and cost savings compared to traditional methods. Watch this video to learn more.

The post A Novel Technology for Post-Library rRNA Depletion first appeared on Bio-Radiations.]]> Choosing Validated Antibodies for Trustworthy Data https://www.bioradiations.com/choosing-validated-antibodies-for-trustworthy-data-122/ Tue, 11 Jan 2022 08:39:11 +0000 https://www.bioradiations.com/?p=12044 The lack of reproducibility in research has been a source of frustration for scientists. Learn why antibody validation is critical for ensuring specificity and what validation methods Bio-Rad is implementing. Our scientists validate the performance of antibodies through CRISPR-Cas9 gene editing by knockout (KO), siRNA knockdown (KD), and immunoprecipitation followed by mass spectrometry (IP-MS).

The post Choosing Validated Antibodies for Trustworthy Data first appeared on Bio-Radiations.]]> A Scalable Immobilized Metal Affinity Chromatography Media for Process Purification https://www.bioradiations.com/a-scalable-immobilized-metal-affinity-chromatography-media-for-process-purification-122/ Tue, 11 Jan 2022 08:38:19 +0000 https://www.bioradiations.com/?p=12042 Downstream process purification of proteins requires a resin with optimized bead size for ideal pressure/flow properties and decent dynamic binding capacity (DBC) that provides production efficiencies and good process economics. Nuvia IMAC Resin provides the mechanical strength, pore structures, ligand density, and particle size distribution required for an operational run at 300 cm/hr with a DBC of 40 mg/ml at 2 bar column backpressure. Protein purification can efficiently be scaled up from milligrams in the lab to kilograms in bioprocess manufacturing. The chemical stability of Nuvia IMAC Resins ensure efficient column regeneration, reproducibility between purification runs, extended column lifetime, and product purity. Watch our video to learn how Nuvia IMAC Resins overcome the challenges associated with process purification of proteins using metal affinity chromatography.

The post A Scalable Immobilized Metal Affinity Chromatography Media for Process Purification first appeared on Bio-Radiations.]]> Resolved — Comic Series https://www.bioradiations.com/resolved-comic-series/ Tue, 11 Jan 2022 07:56:37 +0000 https://www.bioradiations.com/?p=10446 Follow along as a new starry-eyed graduate student learns the ins and outs of life as a research scientist, gaining valuable western blotting tips and tricks. Along for the ride are a seasoned postdoctoral fellow and traditional principal investigator.

The post Resolved — Comic Series first appeared on Bio-Radiations.]]> 5 High-Throughput Screening Applications Using Flow Cytometry https://www.bioradiations.com/5-high-throughput-screening-applications-using-flow-cytometry-921/ Tue, 14 Sep 2021 16:36:30 +0000 https://www.bioradiations.com/?p=11951 For many scientists, high-throughput flow cytometry screens are associated with drug or compound libraries, but did you know that you can also apply this technique to study microorganisms, clinical melanoma samples, and even protein trafficking? Read on for five examples of the use of flow cytometry in diverse, high-throughput screening applications.

The post 5 High-Throughput Screening Applications Using Flow Cytometry first appeared on Bio-Radiations.]]> Beyond the Spec Sheet: Evaluating New qPCR Instrumentation https://www.bioradiations.com/beyond-the-spec-sheet-evaluating-new-qpcr-instrumentation-921/ Tue, 14 Sep 2021 16:36:05 +0000 https://www.bioradiations.com/?p=11937 Whether you are part of a small academic lab or a multinational corporation, upgrading to a new quantitative PCR (qPCR) system can be both exciting and daunting. How will the new system perform compared to your current platform? Will your existing workflows translate smoothly? Read on to learn about our recent bridging study for users considering upgrading to the new CFX Opus Real-Time PCR System.

The post Beyond the Spec Sheet: Evaluating New qPCR Instrumentation first appeared on Bio-Radiations.]]> CDMOs Drive Cell and Gene Therapy from Behind the Scenes https://www.bioradiations.com/cdmos-drive-cell-and-gene-therapy-from-behind-the-scenes-921/ Tue, 14 Sep 2021 16:35:41 +0000 https://www.bioradiations.com/?p=11959 As more of the cell and gene therapies revolutionizing healthcare are approved for use, innovator companies usually get the credit. But many therapies would never make it to market without the expertise and capabilities of contract development and manufacturing organizations (CDMOs) operating behind the scenes. Learn more about how CDMOs enable new cell and gene therapies to come to market, and how Droplet Digital PCR can help.

The post CDMOs Drive Cell and Gene Therapy from Behind the Scenes first appeared on Bio-Radiations.]]> Introducing the GelDoc Go Imaging System https://www.bioradiations.com/introducing-the-geldoc-go-imaging-system-921/ Tue, 14 Sep 2021 16:35:09 +0000 https://www.bioradiations.com/?p=11942 The next iteration of Bio-Rad Gel Documentation Systems has arrived. The GelDoc Go Imaging System gives you a benchtop imaging solution that enables gel documentation in three simple steps. Acquire high-resolution, publication-quality images of nucleic acid gels, protein gels, and colorimetric blots. Image up to four mini gels at once with the large imaging area, and easily set up and control the GelDoc Go Imaging System with Image Lab Touch Software. Read more about the compact, easy-to-use GelDoc Go System.

The post Introducing the GelDoc Go Imaging System first appeared on Bio-Radiations.]]> Bioprocessing Recombinant Proteins https://www.bioradiations.com/bioprocessing-recombinant-proteins-921/ Tue, 14 Sep 2021 16:32:46 +0000 https://www.bioradiations.com/?p=11927 Infectious diseases and cancers are among the main causes of human deaths worldwide. One successful avenue found to treat these diseases is the large-scale production of therapeutic proteins. As a result, the demand for protein expression hosts and accompanying advanced bioprocessing technologies has increased. Read about a workflow for efficient purification of a low-expressing recombinant protein using mixed-mode resins.

The post Bioprocessing Recombinant Proteins first appeared on Bio-Radiations.]]> Two-Step Purification of Low-Expressing Recombinant Exoprotein A Using Mixed-Mode Chromatography https://www.bioradiations.com/two-step-purification-of-low-expressing-recombinant-exoprotein-a-using-mixed-mode-chromatography-921/ Tue, 14 Sep 2021 16:32:01 +0000 https://www.bioradiations.com/?p=11934 Purifying proteins that are not highly expressed can be challenging. Watch this video to learn about a case study demonstrating an efficient workflow for two-step purification of a low-expressing recombinant protein using mixed-mode chromatography.

The post Two-Step Purification of Low-Expressing Recombinant Exoprotein A Using Mixed-Mode Chromatography first appeared on Bio-Radiations.]]> Can You Find All the qPCR-Related Words? https://www.bioradiations.com/can-you-find-all-the-qpcr-related-words-921/ Tue, 14 Sep 2021 16:31:44 +0000 https://www.bioradiations.com/?p=11970 Feeling stressed? Take a break with this qPCR-themed word search.

The post Can You Find All the qPCR-Related Words? first appeared on Bio-Radiations.]]> Top 5 Takeaways from ddPCR World 2021 https://www.bioradiations.com/top-5-takeaways-from-ddpcr-world-2021/ Fri, 06 Aug 2021 18:53:29 +0000 https://www.bioradiations.com/?p=11918 Precision diagnostics for cancer. COVID-19 surveillance in wastewater. Quality control of cell and gene therapies. These were just a few of the cutting-edge applications on display at the Droplet Digital PCR (ddPCR) World 2021 symposium. Recapping the event in 5 takeaways, moderator Francisco Bizouarn shares how international experts are leveraging ddPCR technology to solve today’s scientific and public health problems.

The post Top 5 Takeaways from ddPCR World 2021 first appeared on Bio-Radiations.]]> 6 Tips to Secure Funding for Flow Cytometers https://www.bioradiations.com/6-tips-to-secure-funding-for-flow-cytometers/ Wed, 09 Jun 2021 01:38:24 +0000 https://www.bioradiations.com/?p=11896 We asked core facilities staff and research scientists from the U.S. and Europe for their top tips for securing funding for large instrumentation, such as a cell analyzer or cell sorter. Here are the key questions you should consider while preparing your grant application to increase your chances of a successful outcome.

The post 6 Tips to Secure Funding for Flow Cytometers first appeared on Bio-Radiations.]]> Advancing Molecular Diagnostics for Myelodysplastic Syndrome https://www.bioradiations.com/advancing-molecular-diagnostics-for-myelodysplastic-syndrome/ Wed, 09 Jun 2021 01:03:30 +0000 https://www.bioradiations.com/?p=11885 No patient who has survived cancer wants to hear that they’ve relapsed. But hearing it sooner rather than later can dramatically increase the chances of finding a successful, potentially life-saving treatment. Using next-generation sequencing and Droplet Digital PCR, researchers at the University of Uppsala aim to detect relapse by monitoring patient-specific biomarkers. Read about their ongoing clinical study.

The post Advancing Molecular Diagnostics for Myelodysplastic Syndrome first appeared on Bio-Radiations.]]> Stain-Free Western Blotting: Faster Results, Better Data https://www.bioradiations.com/stain-free-western-blotting-faster-results-better-data/ Wed, 09 Jun 2021 00:52:11 +0000 https://www.bioradiations.com/?p=11878 Western blotting is a well-accepted technique which has a place in every molecular biologist’s toolkit. However, the traditional process is lengthy and filled with manual steps, which can result in variability. Stain-Free technology can shorten your workflow and improve your data. Read our top three reasons to incorporate Stain-Free technology in your western blotting workflow.

The post Stain-Free Western Blotting: Faster Results, Better Data first appeared on Bio-Radiations.]]> Retain Rare Transcripts While Depleting Ribosomal RNA https://www.bioradiations.com/retain-rare-transcripts-while-depleting-ribosomal-rna/ Wed, 09 Jun 2021 00:40:20 +0000 https://www.bioradiations.com/?p=11889 Bio-Rad is excited to announce the launch of SEQuoia RiboDepletion Kit, the newest product in the SEQuoia portfolio of RNA-Seq sample preparation products. This is a stand-alone, post–library depletion kit that eliminates RNA-Seq library fragments that originated from ribosomal RNA sequences. The kit exhibits superior performance in retaining small RNAs while minimizing the loss of rare transcripts and RNA from limited samples, providing a better approach to whole transcriptome profiling. Furthermore, the cost of rRNA depletion can be significantly reduced by pooling and depleting multiple libraries in a single ribodepletion reaction.

The post Retain Rare Transcripts While Depleting Ribosomal RNA first appeared on Bio-Radiations.]]> Advanced Cancer Therapeutics: Increasing Efficacy and Specificity and Reducing Toxicity https://www.bioradiations.com/advanced-cancer-therapeutics-increasing-efficacy-and-specificity-and-reducing-toxicity/ Wed, 09 Jun 2021 00:28:22 +0000 https://www.bioradiations.com/?p=11873 Research into human diseases has led to the development of novel monoclonal antibody drugs. These monoclonal antibody therapies have been successful because of their specificity and selectivity. However, ongoing development and successfully launching a new mAb drug require robust and reliable methods to screen for drug targets, demonstrate drug safety and efficacy, and scale up manufacturing to meet the stringent regulatory guidelines required for approval.

The post Advanced Cancer Therapeutics: Increasing Efficacy and Specificity and Reducing Toxicity first appeared on Bio-Radiations.]]> Single-Step Column Purification for H1N1 VirusUsing Nuvia HP-Q AEX Resin https://www.bioradiations.com/single-step-column-purification-for-h1n1-virususing-nuvia-hp-q-aex-resin/ Wed, 09 Jun 2021 00:01:27 +0000 https://www.bioradiations.com/?p=11869 Learn how a team developed a single-step workflow for column purification of the Influenza A H1N1 subtype using the recently developed Nuvia HP-Q Anion Exchange Resin. This protocol is scalable, efficient, and produced good yields with high purity.

The post Single-Step Column Purification for H1N1 Virus
Using Nuvia HP-Q AEX Resin first appeared on Bio-Radiations.]]> Advantages of Multiplexing with Bio-Plex Assays https://www.bioradiations.com/advantages-of-multiplexing-with-bio-plex-assays/ Tue, 08 Jun 2021 23:53:19 +0000 https://www.bioradiations.com/?p=11867 The Bio-Plex Assay development process is designed to produce high performance multiplex assays. We prioritize sensitivity, broad dynamic range, reproducibility, and shorter incubation times to get you meaningful results faster. Watch this video to learn more about our SMARTS approach to assay development.

The post Advantages of Multiplexing with Bio-Plex Assays first appeared on Bio-Radiations.]]> With Cell and Gene Therapies, Success and Quality Control Go Hand-in-Hand https://www.bioradiations.com/with-cell-and-gene-therapies-success-and-quality-control-go-hand-in-hand/ Tue, 11 May 2021 17:57:30 +0000 https://www.bioradiations.com/?p=11856 While the FDA has approved Abecma, the first CAR-T cell therapy in its class, regulatory approval is just the beginning for cell and gene therapies. Such therapies' safety and financial viability rest on a robust manufacturing and quality control program with the flexibility to scale up and out. Droplet Digital PCR is a sensitive biopharmaceutical quality control tool, offering absolute quantification of nucleic acids and other molecules in a sample.

The post With Cell and Gene Therapies, Success and Quality Control Go Hand-in-Hand first appeared on Bio-Radiations.]]> Western Blot University https://www.bioradiations.com/western-blot-university/ Wed, 21 Apr 2021 18:23:30 +0000 https://www.bioradiations.com/?p=11844 Join us for an in-depth dive into western blotting. Whether you are new to western blotting or an experienced user looking for tips, this five-part webinar series has something for you. Slides and downloadable resources will be available for each webinar. Complete all five classes to receive a certificate of completion and a colorful Western Blotting University poster.

The post Western Blot University first appeared on Bio-Radiations.]]> Immune Surveillance in SARS-CoV-2 Vaccine Development https://www.bioradiations.com/immune-surveillance-in-sars-cov-2-vaccine-development-321/ Mon, 08 Mar 2021 22:14:23 +0000 https://www.bioradiations.com/?p=11818 The ability to characterize the target of cell-mediated immune response is crucial during vaccine development. In studying the characterization of SARS-CoV-2 infection pathogenesis in humans, a research team identified neutralizing antibodies that provided context for vaccine antigen design. Discover how the ZE5 Cell Analyzer was used to detect activation-induced markers, measure intracellular cytokines, and identify spike protein–specific antibodies in a rapid, high-throughput manner.

The post Immune Surveillance in SARS-CoV-2 Vaccine Development first appeared on Bio-Radiations.]]> Introducing the Bio-Plex Pro Human IgG SARS-CoV-2 Serology Assays https://www.bioradiations.com/the-new-bio-plex-human-igg-sars-cov-2-serology-assays-321/ Mon, 08 Mar 2021 22:00:03 +0000 https://www.bioradiations.com/?p=11811 Meet our newest product supporting the fight against SARS-CoV-2: the Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel. This qualitative multiplex immunoassay kit quickly and efficiently detects IgG antibodies against four SARS-CoV-2 antigens (nucleocapsid, receptor binding domain, spike 1, and spike 2).

The post Introducing the Bio-Plex Pro Human IgG SARS-CoV-2 Serology Assays first appeared on Bio-Radiations.]]> Purification of Untagged Proteins Made Attainable for Any Researcher https://www.bioradiations.com/purification-of-untagged-proteins-made-attainable-321/ Mon, 08 Mar 2021 21:45:42 +0000 https://www.bioradiations.com/?p=11801 High purity protein is a common requirement for biochemical and structural studies. A common approach is to recombinantly express an affinity-tagged version of the protein of interest. However, this is not always a viable option. Here we showcase how the NGC Chromatography System and prepacked chromatography columns can be utilized to develop purification workflows for untagged proteins.

The post Purification of Untagged Proteins Made Attainable for Any Researcher first appeared on Bio-Radiations.]]> Countering COVID-19 with the Launch of Two New RT-PCR Kits https://www.bioradiations.com/countering-covid-19-with-the-new-rt-pcr-kits-321/ Mon, 08 Mar 2021 21:24:35 +0000 https://www.bioradiations.com/?p=11792 Bio-Rad is proud to introduce our new RT-PCR testing kits for COVID-19. These all-in-one kits are designed for sensitive, specific detection of SARS-CoV-2 alone, or simultaneous detection of SARS-CoV-2, influenza A, and influenza B. Read on to learn about each kit’s features.

The post Countering COVID-19 with the Launch of Two New RT-PCR Kits first appeared on Bio-Radiations.]]> How FACS, Genetic Screens, and the HAP1 Cell Line Uncover the Biological Mechanisms of Cancer https://www.bioradiations.com/facs-genetic-screens-and-the-hap1-cell-line-in-cancer-research-321/ Mon, 08 Mar 2021 20:17:49 +0000 https://www.bioradiations.com/?p=11786 Genetic screens are powerful tools for finding genes associated with a particular phenotype. They play an especially important role in cancer research and are leveraged to identify predictive biomarkers that can be used to improve treatment efficacy or trace the progression of genetic alterations that ultimately lead to disease. The HAP1 cancer cell line model and fluorescence-activated cell sorting (FACS) bring efficiency to the development of genetic screens that is making them a force to be reckoned with.

The post How FACS, Genetic Screens, and the HAP1 Cell Line Uncover the Biological Mechanisms of Cancer first appeared on Bio-Radiations.]]> The Role of ddPCR in Developing Safe and Effective AAV-Based Gene Therapies https://www.bioradiations.com/the-role-of-ddpcr-in-developing-safe-and-effective-aav-based-gene-therapies/ Mon, 08 Mar 2021 19:46:06 +0000 https://www.bioradiations.com/?p=11782 Developing adeno-associated virus (AAV)-mediated gene therapies requires accurate and precise quality control. Read how Droplet Digital PCR technology provides the sensitivity needed to assess the potential safety and effectiveness of these treatments.

The post The Role of ddPCR in Developing Safe and Effective AAV-Based Gene Therapies first appeared on Bio-Radiations.]]> Immunological Response Factors to SARS-CoV-2 in Acquired Immunity https://www.bioradiations.com/immune-response-and-acquired-immunity-to-sars-cov-2-321/ Mon, 08 Mar 2021 16:11:02 +0000 https://www.bioradiations.com/?p=11824 Multiple publications report variation in the duration of acquired immunity to SARS-CoV-2. In this webinar, Bio-Rad scientists review these reports and discuss factors that influence longevity of acquired immunity. Learn about the mechanisms behind acquired immunity and how cytokine and chemokine activity, preexisting conditions, and more can affect immunity duration.

The post Immunological Response Factors to SARS-CoV-2 in Acquired Immunity first appeared on Bio-Radiations.]]> Automate Your Antibody Purification Workflow at Preparative and Analytical Scale https://www.bioradiations.com/automate-your-antibody-purification-workflow-at-preparative-and-analytical-scale/ Wed, 20 Jan 2021 20:15:19 +0000 https://www.bioradiations.com/?p=11772 Presented by: Katie Schaefer, PhD, Global Product Manager, Bio-Rad
Peter Winship, PhD, Technical Product Manager, Teledyne Cetac Technologies

View on demand

Purification is a multistep process toward confirmation of a target protein’s purity. In this webinar you will learn how to automate your protein purification workflow by following the creation of a hypothetical monoclonal antibody (mAb). Dr. Schaefer and Dr. Winship expertly demonstrate how the NGC Chromatography System and the AEX-500 series Autosampler work together to maximize screening capability as well as rapidly achieve therapeutic development objectives.

The post Automate Your Antibody Purification Workflow at Preparative and Analytical Scale first appeared on Bio-Radiations.]]> Top Questions from Our Wastewater Surveillance Webinar https://www.bioradiations.com/top-questions-from-our-wastewater-surveillance-webinar/ Tue, 08 Dec 2020 18:52:32 +0000 https://www.bioradiations.com/?p=11754 The COVID-19 pandemic has captured the attention of the world and won’t let go. And that has sparked efforts for creative solutions in some surprising places. Wastewater is not where most peoples’ minds go first when they think of airborne diseases, but it holds much promise for tracing and predicting SARS-CoV-2 outbreaks.

The post Top Questions from Our Wastewater Surveillance Webinar first appeared on Bio-Radiations.]]> Inflamed in the Brain: A Protocol for Studying Neuroinflammation in Mouse Brain Tissue https://www.bioradiations.com/inflamed-in-the-brain-a-protocol-for-studying-neuroinflammation-in-mouse-brain-tissue/ Tue, 08 Dec 2020 18:51:33 +0000 https://www.bioradiations.com/?p=11751 Mice are critical model organisms for the study of neuroinflammation. But tissue quantity is often a limiting factor. In a recent paper, researchers describe a protocol for analysis of 33 cytokines and chemokines using only 50 μl of tissue homogenate. The remaining homogenate can be used for RNA extraction or other analysis.

The post Inflamed in the Brain: A Protocol for Studying Neuroinflammation in Mouse Brain Tissue first appeared on Bio-Radiations.]]> Are biofuels good for the planet? https://www.bioradiations.com/are-biofuels-good-for-the-planet/ Tue, 08 Dec 2020 18:51:02 +0000 https://www.bioradiations.com/?p=11748 Will the trend toward using biofuels benefit the earth? Listen in as our Chromies team discusses different types of biofuels and how chromatography plays a role in developing mass produced and energy efficient biofuels.

The post Are biofuels good for the planet? first appeared on Bio-Radiations.]]> Multiplex Immunoassays in the Race for a COVID-19 Vaccine https://www.bioradiations.com/multiplex-immunoassays-in-the-race-for-a-covid-19-vaccine/ Tue, 08 Dec 2020 18:50:06 +0000 https://www.bioradiations.com/?p=11744 In vaccine development, safety and efficacy are critical. Recent studies have shown that multiplex immunoassays offer a flexible way to monitor immune responses. With the ever-increasing pressure on scientists to develop a practical COVID-19 vaccine, multiplex immunoassays stand out as a proven tool.

The post Multiplex Immunoassays in the Race for a COVID-19 Vaccine first appeared on Bio-Radiations.]]> Serving your Downstream Purification Needs https://www.bioradiations.com/serving-your-downstream-purification-needs/ Tue, 08 Dec 2020 18:49:28 +0000 https://www.bioradiations.com/?p=11741 The Process Chromatography Team at Bio-Rad offers a variety of resources to address scientists’ needs during the downstream purification process. Visit our robust online library, which contains application notes, tutorials, webinars, and more, or contact us to speak with a specialist or request a customized seminar.

The post Serving your Downstream Purification Needs first appeared on Bio-Radiations.]]> Top 5 Challenges Faced by AAV Developers Today https://www.bioradiations.com/top-5-challenges-faced-by-aav-developers-today/ Tue, 08 Dec 2020 18:49:01 +0000 https://www.bioradiations.com/?p=11732 Adeno-associated viruses (AAVs) are some of the most commonly used viral vectors for gene therapy, supporting approximately 250 clinical trials to date. However, the manufacturing processes are challenging to scale. Read about five key issues that gene therapy manufacturers who use AAVs need to look out for.

The post Top 5 Challenges Faced by AAV Developers Today first appeared on Bio-Radiations.]]> Introducing the Reliance Select cDNA Synthesis Kit https://www.bioradiations.com/introducing-the-reliance-select-cdna-synthesis-kit/ Tue, 08 Dec 2020 18:47:44 +0000 https://www.bioradiations.com/?p=11725 The Reliance Select cDNA Synthesis Kit is the newest reverse transcription product from Bio-Rad. This high-performance kit features Reliance Reverse Transcriptase, a novel chimeric enzyme that delivers superior results across a broad dynamic range, even with challenging samples. The Reliance Select cDNA Synthesis Kit delivers more accurate reverse transcription quantitative PCR (RT-qPCR) results, even from samples containing PCR inhibitors or degraded RNA.

The post Introducing the Reliance Select cDNA Synthesis Kit first appeared on Bio-Radiations.]]> The Role of Aberrant Cytokine Activity in COVID-19 Disease https://www.bioradiations.com/the-role-of-aberrant-cytokine-activity-in-covid%e2%80%9119-disease/ Mon, 07 Dec 2020 23:15:43 +0000 https://www.bioradiations.com/?p=11716 Presented by: Kenneth Oh, PhD, Collaborations, Applications, and New Technology Senior Global Manager
Kelly King, PhD, Field Application Specialist II
Brad VanderWielen, PhD, Field Application Specialist II
Candice Cox, Global Marketing Manager, Immunoassays

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Emerging reports from the COVID 19 pandemic include a spectrum of patient symptoms due to the host immune response. In this webinar, Bio-Rad scientists discuss early research and nascent hypotheses about the pathophysiology of SARS CoV 2–induced COVID 19 disease by evaluating cytokine and chemokine profiles, the role of chronic inflammation in comorbidities, and the arc of immune resolution of historical virulent pathogens, such as SARS and MERS.

The post The Role of Aberrant Cytokine Activity in COVID-19 Disease first appeared on Bio-Radiations.]]> A New International Standard to Measure DNA? https://www.bioradiations.com/a-new-international-standard-to-measure-dna/ Wed, 09 Sep 2020 22:36:43 +0000 https://www.bioradiations.com/?p=11665 From quantifying a patient’s tumor DNA to counting the viral load of an individual infected with COVID-19, the breadth of digital PCR (dPCR) applications continues to grow and fulfill critical scientific needs. dPCR offers the ability to count nucleic acid molecules directly, rather than extrapolating the quantity from standard curves as with many other methods. By harmonizing diagnostic procedures on dPCR platforms, the scientific and medical communities can become more coordinated and efficient.

The post A New International Standard to Measure DNA? first appeared on Bio-Radiations.]]> Data Standardization https://www.bioradiations.com/data-standardization/ Wed, 09 Sep 2020 22:12:05 +0000 https://www.bioradiations.com/?p=11661 Increasingly, biopharma research & development experimental data are siloed and stored in varying formats. This makes it difficult to search, retrieve and share data. Furthermore, these data are often not interoperable, making it impossible to compare. Data needs to be better managed to be more collaborative, shareable, and interoperable, in order to accelerate research and continue making breakthroughs. Data standardization aims to forge a path toward machine-readable and actionable data that can be found, accessed, and reused with minimal human intervention.

The post Data Standardization first appeared on Bio-Radiations.]]> 7 Tips for Purchasing Your Next Cell Sorter https://www.bioradiations.com/7-tips-next-cell/ Wed, 09 Sep 2020 22:01:53 +0000 https://www.bioradiations.com/?p=11656 Presented here are some tips to help you avoid buyer’s remorse and make an educated decision about which instrument to purchase. These tips provide you with the tools to make an informed decision when purchasing a cell sorter.

The post 7 Tips for Purchasing Your Next Cell Sorter first appeared on Bio-Radiations.]]> Introducing the CFX Opus Real-Time PCR Systems — A Modern Take on Bio-Rad Consistency https://www.bioradiations.com/introducing-the-cfx-opus-real-time-pcr-systems-a-modern-take-on-bio-rad-consistency/ Wed, 09 Sep 2020 21:55:33 +0000 https://www.bioradiations.com/?p=11674 Coming soon: The next iteration in Bio-Rad’s tradition of qPCR excellence. The compact CFX Opus Real-Time PCR System is more connected, consistent, and easier to use than ever before, and is available in 96- and 384-well formats. The CFX Opus Systems launch with our new BR.io cloud platform and the latest version of CFX Maestro Software, for complete instrument control and data analysis capabilities.

The post Introducing the CFX Opus Real-Time PCR Systems —
A Modern Take on Bio-Rad Consistency first appeared on Bio-Radiations.]]> Sorting an Old Problem: Are Cells Stressed by Fluorescence-Activated Cell Sorting? https://www.bioradiations.com/sorting-an-old-problem-are-cells-stressed-by-fluorescence-activated-cell-sorting/ Wed, 09 Sep 2020 21:16:22 +0000 https://www.bioradiations.com/?p=11642 Fluorescence-activated cell sorting is a valuable tool for research, but could the physical forces of sorting stress the cells, altering their behavior and confounding experimental results? Recent research found that although cell sorting activated p38 MAPK stress signaling, no functional or structural changes were observable. The results indicate that jet-in-air systems such as the Bio-Rad S3e Cell Sorter do not alter cell behavior.

The post Sorting an Old Problem: Are Cells Stressed by Fluorescence-Activated Cell Sorting? first appeared on Bio-Radiations.]]> How to Make a Successful Vaccine https://www.bioradiations.com/how-to-make-a-successful-vaccine/ Wed, 09 Sep 2020 21:05:12 +0000 https://www.bioradiations.com/?p=11636 As phase 3 clinical trials of investigational vaccines for COVID-19 begin in the U.S., you may be wondering what it takes to make an effective vaccine? In case you missed it, check out our Hanging with My Chromies podcast, episodes 6 and 10 on vaccine development and viral spread, and learn about the role chromatography plays in making a successful vaccine.

The post How to Make a Successful Vaccine first appeared on Bio-Radiations.]]> 10 Tips for Setting Up a Successful Cell Sorting Experiment https://www.bioradiations.com/10-tips-cell-sorting/ Wed, 10 Jun 2020 17:59:14 +0000 https://www.bioradiations.com/?p=11569 Incorporating fluorescence-based cell sorting into your lab grants you powerful access to new research areas and rarer cell types without sacrificing efficiency or results. New, powerful techniques like CRISPR and single-cell analysis are enhanced by the isolation of specific cells. But isolating cell populations of the right quality and quantity is both challenging and time consuming. We offer some tips to set yourself up for success and get the most out of your sorts.

The post 10 Tips for Setting Up a Successful Cell Sorting Experiment first appeared on Bio-Radiations.]]> Globally Aware Genomes — an Informatics Challenge https://www.bioradiations.com/globally-aware-genomes-an-informatics-challenge/ Wed, 10 Jun 2020 17:50:49 +0000 https://www.bioradiations.com/?p=11574 The Human Genome Project was completed nearly two decades ago, in 2003. The primary vision of this project was to produce a human reference genome, a representative example of the set of genes in one idealized individual human. However, the currently available reference genomes do not represent the genetic diversity found across different human populations. As such, any downstream products (drugs therapies, personalized medicine, etc.) derived from the human genome will not accurately represent the vast majority of the human population.

The post Globally Aware Genomes — an Informatics Challenge first appeared on Bio-Radiations.]]> Expert Coffee Chats: Coffee Chat #4 – Droplet Digital PCR in Cell and Gene Therapy https://www.bioradiations.com/expert-coffee-chats-coffee-chat-4-droplet-digital-pcr-in-cell-and-gene-therapy/ Wed, 10 Jun 2020 17:41:19 +0000 https://www.bioradiations.com/?p=11564 Presented by: Matthew L. Turner, PhD, Field Application Scientist - ddPCR, NGC and qPCR, Tara Ellison, PhD, Senior Field Application Scientist

Date: June 17, 2020
Time: 9 AM, U.S. Pacific time

  • Robust methodology for viral titer using ddPCR
  • Precise copy number determination & improved confidence in acceptance criteria
  • Enhance your throughput and minimize hands-on time
  • Learn how ddPCR is used in other key applications
The post Expert Coffee Chats: Coffee Chat #4 – Droplet Digital PCR in Cell and Gene Therapy first appeared on Bio-Radiations.]]> Analyzing Carbohydrates https://www.bioradiations.com/analyzing-carbohydrates/ Wed, 10 Jun 2020 17:32:13 +0000 https://www.bioradiations.com/?p=11560 Carbohydrates are among the most important components of foods, providing energy as well as flavor, texture, and other physical properties. Identification and quantification of carbohydrates in food is commonly performed using high-performance liquid chromatography. Bio-Rad Aminex Ion Exchange Resins have become the industry standard for carbohydrate and organic acid analysis.

The post Analyzing Carbohydrates first appeared on Bio-Radiations.]]> A Novel CAR-T Cell Therapy Approach Using Fluorescence-Activated Cell Sorting and Stem Cell Transplantation https://www.bioradiations.com/a-novel-car-t-cell-therapy-approach-using-fluorescence-activated-cell-sorting-and-stem-cell-transplantation/ Wed, 10 Jun 2020 17:21:16 +0000 https://www.bioradiations.com/?p=11555 Successful chimeric antigen receptor- (CAR-) T cell therapy requires a target antigen that is unique to cancer cells. But what happens when there are no unique antigens? Researchers at Columbia University Medical Center addressed this problem by replacing healthy non-target cells with genetically modified versions lacking the CAR-T cell target. Their results, published in PNAS, may provide a new avenue for treatment of some types of cancer.

The post A Novel CAR-T Cell Therapy Approach Using Fluorescence-Activated Cell Sorting and Stem Cell Transplantation first appeared on Bio-Radiations.]]> Improving CRISPR-Cas9 Gene Editing:Tackling the HDR Inefficiency Problem https://www.bioradiations.com/improving-crispr-cas9-gene-editingtackling-the-hdr-inefficiency-problem/ Thu, 12 Mar 2020 00:04:27 +0000 https://www.bioradiations.com/?p=11512 A recent publication showed that expression of a protein involved in homology-directed repair (HDR) can improve efficiency of CRISPR-Cas9 gene editing. Learn how manipulation of DNA repair pathways and improvements to gene editing workflows, such as the addition of cell sorting, can facilitate applications of CRISPR-Cas9 technology.

The post Improving CRISPR-Cas9 Gene Editing:
Tackling the HDR Inefficiency Problem first appeared on Bio-Radiations.]]> Lab of the Future https://www.bioradiations.com/lab-of-the-future/ Wed, 11 Mar 2020 16:58:22 +0000 https://www.bioradiations.com/?p=11509 In an effort to increase the return on investment of research and development, pharmaceutical companies are constantly looking for ways to reduce costs. One way to do this is to incorporate technology and minimize human interaction. As a result, scientists will have more time to focus on the design and analysis of experiments. How will the Lab of the Future reflect these changes?

The post Lab of the Future first appeared on Bio-Radiations.]]> Active Machine Learning in Drug Discovery https://www.bioradiations.com/active-machine-learning-in-drug-discovery/ Wed, 12 Feb 2020 21:48:31 +0000 https://www.bioradiations.com/?p=11494 Drug discovery and development are both time and resource intensive since a lot of different compounds need to be screened across a lot of different targets. See how machine learning models can be used to predict screening results and how active learning algorithms can be used to efficiently select which experiments to perform in order to build those models.

The post Active Machine Learning in Drug Discovery first appeared on Bio-Radiations.]]> Reference Gene Selection Using PrimePCR Plates and CFX Maestro Software https://www.bioradiations.com/reference-gene-selection-using-primepcr-plates-and-cfx-maestro-software/ Wed, 12 Feb 2020 21:13:21 +0000 https://www.bioradiations.com/?p=11492 Reference gene selection and validation are very important for gene expression studies that involve different samples or experimental conditions and therefore are absolutely necessary before any further exploration. See how PrimePCR Reference Gene Selection Panels and CFX Maestro Software provide an easy-to-use system with which to select appropriate reference genes.

The post Reference Gene Selection Using PrimePCR Plates and CFX Maestro Software first appeared on Bio-Radiations.]]> Biosimilar Purification with Ion Exchange and Mixed-Mode Resins https://www.bioradiations.com/biosimilar-purification-with-ion-exchange-and-mixed-mode-resins/ Wed, 12 Feb 2020 20:43:12 +0000 https://www.bioradiations.com/?p=11478 Presented by: Randy Drevland, PhD, Senior Applications Scientist

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Dr. Drevland presents a viable alternative workflow for the development of a HUMIRA-like biosimilar using IEX and mixed-mode resins.

The post Biosimilar Purification with Ion Exchange and Mixed-Mode Resins first appeared on Bio-Radiations.]]> GMP-Compliant Production of CAR-T Cells Using mRNA Transfection https://www.bioradiations.com/gmp-compliant-production-of-car-t-cells-using-mrna-transfection/ Wed, 15 Jan 2020 22:43:59 +0000 https://www.bioradiations.com/?p=11462 Many patients with treatment-resistant blood cancers have found new hope in chimeric antigen receptor (CAR)-T cell therapy. But generation of CAR-T cells at clinical scale can be challenging. Find out how the Gene Pulser Xcell Electroporation System can be used to reduce costs and risks associated with clinical-scale CAR-T cell production, while maintaining GMP compliance.

The post GMP-Compliant Production of CAR-T Cells Using mRNA Transfection first appeared on Bio-Radiations.]]> Data Integration and Visualization Approaches https://www.bioradiations.com/data-integration-and-visualization-approaches/ Wed, 15 Jan 2020 22:32:33 +0000 https://www.bioradiations.com/?p=11456 Over the past several decades, research informatics (RIS) environments within BioPharma organizations have evolved dramatically, increasing the demand for simple and easy data integration and visualization. Learn about the tools available to address this need and what the future could hold.

The post Data Integration and Visualization Approaches first appeared on Bio-Radiations.]]> Turning Back the Clock on Mouse Neural Progenitor Cells https://www.bioradiations.com/turning-back-the-clock-on-mouse-neural-progenitor-cells/ Wed, 15 Jan 2020 22:25:36 +0000 https://www.bioradiations.com/?p=11452 Is the fate of an embryonic cell determined by the age of the cell or the age of the embryo? To explore this question, researchers isolated and transplanted live neural cells from mouse embryos at different stages of development. Learn about their findings and how the S3e Cell Sorter helped achieve this incredible feat.

The post Turning Back the Clock on Mouse Neural Progenitor Cells first appeared on Bio-Radiations.]]> A Powerful Solution for NGS RNA Library Preparation https://www.bioradiations.com/a-powerful-solution-for-ngs-rna-library-preparation/ Wed, 15 Jan 2020 22:13:41 +0000 https://www.bioradiations.com/?p=11442 The SEQuoia Complete Stranded RNA Library Prep Kit is the newest addition to Bio-Rad’s next-generation sequencing (NGS) space. Capturing long and short RNAs and validated with FFPE and low-quality samples, see why it should be your top choice for high-performance RNA-Seq library prep.

The post A Powerful Solution for NGS RNA Library Preparation first appeared on Bio-Radiations.]]> Artificial Intelligence in Drug Development https://www.bioradiations.com/artificial-intelligence-in-drug-development/ Thu, 12 Dec 2019 00:22:08 +0000 https://www.bioradiations.com/?p=11412 Artificial intelligence (AI) relies on building a model and applying it to solve a specific problem. Read how AI is being used to accelerate research and development, process development, manufacturing, and clinical trials within genomics.

The post Artificial Intelligence in Drug Development first appeared on Bio-Radiations.]]> Hanging with My Chromies Podcast Series https://www.bioradiations.com/hanging-with-my-chromies-podcast-series/ Thu, 12 Dec 2019 00:04:21 +0000 https://www.bioradiations.com/?p=10437 The Hanging with My Chromies podcast series is focused on the many surprising applications of chromatography and the stories surrounding them. Join us every month to see what you can learn.

The post Hanging with My Chromies Podcast Series first appeared on Bio-Radiations.]]> Maximizing the Efficiency of Production Workflows with NGC Chromatography Systems https://www.bioradiations.com/maximizing-the-efficiency-of-production-workflows-with-ngc-chromatography-systems/ Thu, 12 Dec 2019 00:00:51 +0000 https://www.bioradiations.com/?p=11403 See how an antibody expression service provider, Evitria (Zurich, Switzerland) optimized and expanded their antibody production services using the NGC Chromatography Systems to meet the growing demands of their customers.

The post Maximizing the Efficiency of Production Workflows with NGC Chromatography Systems first appeared on Bio-Radiations.]]> Integrating Viral Clearance into Your Process Development — A Case Study Using a Mixed‑Mode Chromatography Resin https://www.bioradiations.com/integrating-viral-clearance-into-your-process-development/ Wed, 13 Nov 2019 21:18:55 +0000 https://www.bioradiations.com/?p=11366 Presented by: Akunna Iheanacho, Director of Research and Development at Texcell – North America
Xuemei He, R&D Manager, Chromatograpy Media Chemistry at Bio-Rad Laboratories

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Learn about evaluating viral clearance using a DOE approach with a mixed-mode chromatography resin.

The post Integrating Viral Clearance into Your Process Development — A Case Study Using a Mixed‑Mode Chromatography Resin first appeared on Bio-Radiations.]]> Cloud Migration Strategies for BioPharma https://www.bioradiations.com/cloud-migration-strategies-for-biopharma/ Wed, 13 Nov 2019 21:15:23 +0000 https://www.bioradiations.com/?p=11378 Many BioPharma organizations are moving to the cloud in order to improve the performance and speed, cost, physical and personnel resources, security, back up, and sharing/collaboration associated with large, complex datasets. Learn about the different strategies used in migrating to the cloud.

The post Cloud Migration Strategies for BioPharma first appeared on Bio-Radiations.]]> Protein Thermal Shift Assays Made Easy with Bio-Rad’s Family of CFX Real-Time PCR Detection Systems https://www.bioradiations.com/protein-thermal-shift-assays-made-easy-with-bio-rads-family-of-cfx-real-time-pcr-detection-systems/ Wed, 13 Nov 2019 21:12:22 +0000 https://www.bioradiations.com/?p=11350 Protein thermal shift assays enable quick and easy buffer optimization for increased protein stability. See how Bio-Rad’s family of CFX Real-Time PCR Detection Systems can measure protein thermal stability with higher throughput and more buffer systems than traditional circular dichroism detection.

The post Protein Thermal Shift Assays Made Easy with
Bio-Rad’s Family of CFX Real-Time PCR Detection Systems first appeared on Bio-Radiations.]]> Challenging the Microbial Sanitization of a Liquid Chromatography System https://www.bioradiations.com/challenging-the-microbial-sanitization-of-a-liquid-chromatography-system/ Wed, 13 Nov 2019 21:07:31 +0000 https://www.bioradiations.com/?p=11330 When developing biologics or biotherapeutics, it is imperative that no contaminating or hazardous residual components from the organisms responsible for protein expression remain after purification. See how the NGC Chromatography System was challenged with microbes and efficiently sanitized, making it ideal for the small-scale production of biotherapeutics.

The post Challenging the Microbial Sanitization of a Liquid Chromatography System first appeared on Bio-Radiations.]]> Episode 1: Moving to the Cloud https://www.bioradiations.com/episode-1-moving-to-the-cloud/ Tue, 08 Oct 2019 14:28:30 +0000 http://BioradiationsBioRadioPodcastSeriesEpisodeOne Listen to the podcast. PROLOGUE Charlene Son Rigby: They are really looking to get their test validated and launch their test as quickly as possible. Being able to do that ...

The post Episode 1: Moving to the Cloud first appeared on Bio-Radiations.]]> Why are research organizations moving to the Cloud? Learn why they decided to initiate the move, what issues they’ve tackled, what their biggest challenges were, and where they’re going next. Why are research organizations moving to the Cloud? Learn why they decided to initiate the move, what issues they’ve tackled, what their biggest challenges were, and where they’re going next. Bio-Rad Laboratories, Inc. full false 21:50 <iframe src="https://player.blubrry.com/?media_url=https%3A%2F%2Fmedia.blubrry.com%2Fbiorad_io%2Fcontent.blubrry.com%2Fbiorad_io%2FBioRad_io_Podcast_Series_Episode_One.mp3&amp;podcast_link=https%3A%2F%2Fwww.bioradiations.com%2Fepisode-1-moving-to-the-cloud%2F&amp;artwork_url=https%3A%2F%2Fwww.bioradiations.com%2Fpodcasts%2Fbioradio%2Fbioradio_podsq_1400x1400.jpg#mode-Light&border-000000&progress-000000" scrolling="no" width="100%" height="165" frameborder="0" id="blubrryplayer-1" class="blubrryplayer" title="Blubrry Podcast Player"></iframe> Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR https://www.bioradiations.com/do-more-with-less-five-steps-to-great-results-using-one-step-multiplex-rt-qpcr/ Wed, 11 Sep 2019 18:17:59 +0000 https://www.bioradiations.com/?p=11238 One-step multiplex RT-qPCR is a technique used to rapidly quantify multiple targets directly from RNA in a single reaction. But proper optimization and validation is essential for its success. These five tips will have you designing primers and probes, selecting reporters, and validating and optimizing your experiments like a pro.

The post Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR first appeared on Bio-Radiations.]]> Expanded Droplet Digital PCR Multiplexing Capability Using Two Different Strategies https://www.bioradiations.com/expanded-droplet-digital-pcr-multiplexing-capability-using-two-different-strategies/ Wed, 11 Sep 2019 17:38:33 +0000 https://www.bioradiations.com/?p=11221 Multiplexing with Droplet Digital PCR (ddPCR) allows for improved sensitivity and precision, as well as increased low-level detection in limited sample types like liquid biopsies. Learn about amplitude and probe-mixing multiplexing and how our ddPCR Assays can help set you up for multiplexing success.

The post Expanded Droplet Digital PCR Multiplexing Capability Using Two Different Strategies first appeared on Bio-Radiations.]]> Fluorescent Immunoblots and Multiplex Analysis https://www.bioradiations.com/fluorescent-immunoblots-and-multiplex-analysis/ Wed, 11 Sep 2019 16:20:32 +0000 https://www.bioradiations.com/?p=11218 Presented by: Gary F. Ross, PhD, Proteomics Field Application Scientist

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Learn best practices for fluorescent western blotting, including multiplex analysis and image acquisition.

The post Fluorescent Immunoblots and Multiplex Analysis first appeared on Bio-Radiations.]]> Purification of Antibodies with Nuvia aPrime 4A Hydrophobic Anion Exchange Resin https://www.bioradiations.com/purification-of-antibodies-with-nuvia-aprime-4a-hydrophobic-anion-exchange-resin/ Tue, 13 Aug 2019 23:33:43 +0000 https://www.bioradiations.com/?p=11173 Our new hydrophobic anion exchange mixed-mode resin, Nuvia aPrime 4A, has been developed to overcome existing challenges in antibody purification. See how we purified an acidic and basic antibody through bind-elute and flow-through modes of purification, respectively, and helped to improve process productivity and economy.

The post Purification of Antibodies with Nuvia aPrime 4A Hydrophobic Anion Exchange Resin first appeared on Bio-Radiations.]]> Tyrosine Phosphorylation of EGF R https://www.bioradiations.com/tyrosine-phosphorylation-of-egf-r/ Tue, 13 Aug 2019 20:57:17 +0000 https://www.bioradiations.com/?p=11158 Growth factors are protein signaling molecules that play crucial roles in the regulation of numerous cellular processes such as proliferation, differentiation, and survival. Learn more about the phosphorylation of one such factor, the epidermal growth factor receptor (EGF R).

The post Tyrosine Phosphorylation of EGF R first appeared on Bio-Radiations.]]> Sensitive Codetection of Viral-Pathogen RNA and DNA Using One-Step Multiplex RT-qPCR https://www.bioradiations.com/sensitive-codetection-of-viral-pathogen-rna-and-dna-using-one-step-multiplex-rt-qpcr/ Tue, 09 Jul 2019 23:29:07 +0000 https://www.bioradiations.com/?p=10980 RT-qPCR is a well-established method for the detection, quantification, and typing of different microbial agents. However, when dealing with the challenges of pathogen detection, not all RT-qPCR reagents are created equal. See how we used a novel one-step multiplex RT-qPCR supermix to demonstrate sensitive codetection of viral RNA and DNA targets in a multiplex setting.

The post Sensitive Codetection of Viral-Pathogen RNA and DNA
Using One-Step Multiplex RT-qPCR first appeared on Bio-Radiations.]]> End the Cycle of Repeated, Failed Experiments with a Robust Field Guide to qPCR https://www.bioradiations.com/end-the-cycle-of-repeated-failed-experiments-with-a-robust-field-guide-to-qpcr/ Wed, 12 Jun 2019 00:46:09 +0000 https://www.bioradiations.com/?p=10902 qPCR has become ubiquitous for nucleic acid detection and quantification and remains the gold standard for a wide array of applications. To help you publish high-quality, reproducible data that reflect true experimental conditions, we’ve developed this comprehensive guide to performing the ultimate qPCR experiment.

The post End the Cycle of Repeated, Failed Experiments with a Robust Field Guide to qPCR first appeared on Bio-Radiations.]]> A New AEX Mixed-Mode Chromatography Resin for High Selectivity and Recovery https://www.bioradiations.com/a-new-aex-mixed-mode-chromatography-resin-for-high-selectivity-and-recovery/ Tue, 11 Jun 2019 20:26:50 +0000 https://www.bioradiations.com/?p=10889 Presented by: Xuemei He, PhD, R&D Manager, Chromatography Media Chemistry, Bio-Rad Laboratories

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This webinar highlights the properties of Bio-Rad’s newest hydrophobic AEX chromatography resin, Nuvia aPrime 4A, which is designed to facilitate selective and reversible binding of target molecules for higher purity and recovery over traditional chromatography resins.

The post A New AEX Mixed-Mode Chromatography Resin for High Selectivity and Recovery first appeared on Bio-Radiations.]]> New Product Feature: Multiplex RT-qPCR in One Step https://www.bioradiations.com/new-product-feature-multiplex-rt-qpcr-in-one-step/ Tue, 14 May 2019 20:14:19 +0000 https://www.bioradiations.com/?p=10808 Using RT-qPCR for RNA, though critical in several industries, is often met with challenges. Many current options don't allow for multiplexing or high-throughput operation. Bio-Rad's new Reliance One-Step Multiplex Supermix was designed to grant researchers the expanded, efficient capabilities they need in RT-qPCR. This article presents some of the performance data from the new supermix and describes how exceptional it is at multiplex detection.

The post New Product Feature: Multiplex RT-qPCR in One Step first appeared on Bio-Radiations.]]> Advancements in Western Blotting Technology: A Western Workflow That Delivers More Precise, Accurate, and Reproducible Results https://www.bioradiations.com/advancements-in-western-blotting-technology/ Wed, 10 Apr 2019 19:33:17 +0000 https://www.bioradiations.com/?p=10701 Presented by: Sean C. Taylor, MBA, PhD, Field Application Scientist Manager

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Learn how to produce journal-ready reproducible and quantitative western blot data.

The post Advancements in Western Blotting Technology: A Western Workflow That Delivers More Precise, Accurate, and Reproducible Results first appeared on Bio-Radiations.]]> Discover Our Capabilities https://www.bioradiations.com/discover-our-capabilities/ Wed, 10 Apr 2019 18:16:19 +0000 https://www.bioradiations.com/?p=10698 From flow cytometry to chromatography to western blot imaging to PCR plastics, learn more about Bio-Rad and see what’s possible in your research.

The post Discover Our Capabilities first appeared on Bio-Radiations.]]> Stop Compromising between High Yield and High Purity in Downstream Purifications https://www.bioradiations.com/stop-compromising-between-high-yield-and-high-purity-in-downstream-purifications/ Tue, 12 Mar 2019 23:59:26 +0000 https://www.bioradiations.com/?p=10596 When it comes to downstream protein purifications, does it feel like you’re in a constant tug of war between high yield and high purity? With Bio-Rad’s Nuvia aPrime 4A Resin, you don’t have to choose. With both ion exchange and mixed-mode properties, see how the resin is able to deliver simultaneous purity and yield, for even difficult-to-purify proteins.

The post Stop Compromising between High Yield and High Purity in Downstream Purifications first appeared on Bio-Radiations.]]> Maximizing Your Mouse: Using the Power of Bio‑Plex to Analyze Mouse Models of Disease and Treatment https://www.bioradiations.com/maximizing-your-mouse-using-the-power-of-bio%e2%80%91plex-to-analyze-mouse-models-of-disease-and-treatment/ Tue, 12 Mar 2019 21:16:30 +0000 https://www.bioradiations.com/?p=10574 Presented by: Daniel Braunschweig, PhD, Proteomics Field Application Scientist Leader

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This webinar provides an overview of multiplexing technology for research use with mouse samples and highlights the associated benefits to your drug discovery workflow. Specific topics include:

The post Maximizing Your Mouse: Using the Power of Bio‑Plex to Analyze Mouse Models of Disease and Treatment first appeared on Bio-Radiations.]]> Changing the Treatment Landscape with the Development of Biologics https://www.bioradiations.com/changing-the-treatment-landscape-with-the-development-of-biologics/ Wed, 13 Feb 2019 21:49:54 +0000 https://www.bioradiations.com/?p=10525 Biologics are transforming the healthcare landscape and bringing us closer to the realization of personalized solutions to our health problems. Learn about what biologics are and how protein purification plays a key role in their overall success as a therapeutic.

The post Changing the Treatment Landscape with the Development of Biologics first appeared on Bio-Radiations.]]> 10 Tips for Western Blot Detection of Phosphorylation Events https://www.bioradiations.com/10-tips-for-western-blot-detection-of-phosphorylation-events/ Wed, 13 Feb 2019 18:21:10 +0000 https://www.bioradiations.com/?p=10513 Being able to interrogate protein phosphorylation events and aberrant phosphorylation levels is critical to our understanding of various biological and pathological processes. However, detection of phosphorylated proteins in western blots can be challenging. These ten tips will help you generate robust data and cleaner blots.

The post 10 Tips for Western Blot Detection of Phosphorylation Events first appeared on Bio-Radiations.]]> A Model System for Fast Screening of Optimal Protein Purification Conditions https://www.bioradiations.com/a-model-system-for-fast-screening-of-optimal-protein-purification-conditions/ Wed, 09 Jan 2019 18:12:36 +0000 https://www.bioradiations.com/?p=10441 See how you can efficiently reduce identification of optimal protein purification conditions from five days to just 18 hours. All you need is a DOE model and the Multivariable Scouting tool and automation abilities of the NGC Chromatography System.

The post A Model System for Fast Screening of Optimal Protein Purification Conditions first appeared on Bio-Radiations.]]> Develop an Immunotherapy in Augmented Reality https://www.bioradiations.com/develop-an-immunotherapy-in-augmented-reality/ Wed, 09 Jan 2019 18:10:23 +0000 https://www.bioradiations.com/?p=10439 Every year cancer kills eight million people worldwide. By 2030, the number of annually diagnosed cases will exceed 21.7 million resulting in 13 million cancer deaths due to aging and population growth. Join the fight against cancer by embarking on a research mission to develop a personalized immunotherapy using augmented reality.

The post Develop an Immunotherapy in Augmented Reality first appeared on Bio-Radiations.]]> Moving Forward with Immunotherapies for Cancer https://www.bioradiations.com/moving-forward-with-immunotherapies-for-cancer/ Wed, 12 Dec 2018 18:07:41 +0000 http://www.bioradiations.com/?p=10384 Frost & Sullivan, a business consulting firm involved in market research and analysis, conducted a thought leadership forum that brought together experts in the field of immuno-oncology. Follow their discussions about the current state of immunotherapies and the key challenges and future approaches to the targeted use of the immune system in cancer treatment.

The post Moving Forward with Immunotherapies for Cancer first appeared on Bio-Radiations.]]> One for All — The Universal qPCR Reagent https://www.bioradiations.com/one-for-all-the-universal-qpcr-reagent/ Tue, 11 Dec 2018 20:29:18 +0000 http://www.bioradiations.com/?p=10369 Passive reference dyes are included in qPCR/RT-qPCR supermixes to compensate for pipetting errors and thermal and/or optical differences in real-time instruments. Our universal line of reagents eliminates the need for spiking in dyes or having to worry about how much ROX your instrument needs. See how your qPCR experiments can also generate more reliable data and be reproducible by anyone.

The post One for All — The Universal qPCR Reagent first appeared on Bio-Radiations.]]> Organs-On-Chips: Solving Persistent Pharmacokinetic Problems https://www.bioradiations.com/organs-on-chips-solving-persistent-pharmacokinetic-problems/ Tue, 11 Dec 2018 19:31:49 +0000 http://www.bioradiations.com/?p=10367 Mock organs the size of a thumb drive are poised to revolutionize drug development and discovery. Dr. Dan Tagle at the NIH, the latest guest on Brilliant Science, specializes in divining new ways to innovate organs-on-chips, and he's even sending them to space. Read more about this growing technology and its fascinating applications.

The post Organs-On-Chips: Solving Persistent Pharmacokinetic Problems first appeared on Bio-Radiations.]]> Biomarkers for the Brain https://www.bioradiations.com/biomarkers-for-the-brain/ Wed, 14 Nov 2018 20:07:35 +0000 http://www.bioradiations.com/?p=10338 Frost & Sullivan, a business consulting firm involved in market research and analysis, conducted a thought leadership forum that brought together experts in the field of CNS biomarkers. Follow their discussions about technological advancements in the field and future implications of the current research and development.

The post Biomarkers for the Brain first appeared on Bio-Radiations.]]> Brilliant Science Podcast Series https://www.bioradiations.com/brilliant-science-podcast-series/ Wed, 14 Nov 2018 20:05:09 +0000 http://www.bioradiations.com/?p=9697 The Brilliant Science podcasts will introduce you to some of the amazing science being done around the world. Join us every month to see what you can learn.

The post Brilliant Science Podcast Series first appeared on Bio-Radiations.]]> Discover lncRNA: Understanding the Long Noncoding Transcriptome https://www.bioradiations.com/discover-lncrna-understanding-the-long-noncoding-transcriptome/ Wed, 14 Nov 2018 20:04:35 +0000 http://www.bioradiations.com/?p=10348 Learn about the exciting new field of long noncoding RNA (lncRNA) and how this newly discovered class of RNA compares to messenger RNA (mRNA).

The post Discover lncRNA: Understanding the Long Noncoding Transcriptome first appeared on Bio-Radiations.]]> See the Signal — Illuminating the Pathway to Confident Western Blot Detection of Phosphorylated Proteins https://www.bioradiations.com/see-the-signal-illuminating-the-pathway-to-confident-western-blot-detection-of-phosphorylated-proteins/ Wed, 14 Nov 2018 20:03:36 +0000 http://www.bioradiations.com/?p=10329 Presented by: Rachael Preston, PhD, Applications Scientist, Bio-Rad Laboratories

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The most common method for assessing the phosphorylation state of a protein is western blotting. In this webinar we talk you through how to fine-tune your western blot protocol to detect phosphorylation events and generate publication-quality western blots.

The post See the Signal — Illuminating the Pathway to Confident Western Blot Detection of Phosphorylated Proteins first appeared on Bio-Radiations.]]> Alzheimer’s Disease: A Smoking Gun with No Suspect https://www.bioradiations.com/alzheimers-disease-a-smoking-gun-with-no-suspect/ Tue, 09 Oct 2018 23:19:33 +0000 http://www.bioradiations.com/?p=10188 For the past 30 years, there has been one assumed culprit of Alzheimer’s disease (AD) — beta-amyloid. However, researchers have been unsuccessful in developing effective therapies targeting this protein. Understand the history of AD and beta-amyloid and see what other biomarkers are being investigated as potential therapeutic targets.

The post Alzheimer’s Disease: A Smoking Gun with No Suspect first appeared on Bio-Radiations.]]> Six Tips to Improve Your Co-IP Results https://www.bioradiations.com/six-tips-to-improve-your-co-ip-results/ Tue, 09 Oct 2018 23:18:56 +0000 http://www.bioradiations.com/?p=10186 Co-immunoprecipitation (co-IP) is a great way to identify target protein complexes and interacting partners. Though the technique can be highly informative, it is also challenging to master. The six tips and protocol outlined in this article will help you achieve successful co-IP results.

The post Six Tips to Improve Your Co-IP Results first appeared on Bio-Radiations.]]> Single-Step Influenza and Dengue Virus Purification with Mixed-Mode CHT Ceramic Hydroxyapatite XT Media https://www.bioradiations.com/single-step-influenza-and-dengue-virus-purification-with-mixed-mode-cht-ceramic-hydroxyapatite-xt-media/ Tue, 09 Oct 2018 23:18:21 +0000 http://www.bioradiations.com/?p=10181 See how you can use CHT XT Mixed-Mode Media to recover over 75% of your virus in a single purification step. All this while reducing contaminating proteins by over 90% and removing over 60% of dsDNA. Get simple, fast, and reproducible viral purification with CHT XT.

The post Single-Step Influenza and Dengue Virus Purification with Mixed-Mode CHT Ceramic Hydroxyapatite XT Media first appeared on Bio-Radiations.]]> Bio-Plex Manager Software Overview https://www.bioradiations.com/bio-plex-manager-software-overview/ Tue, 09 Oct 2018 23:16:19 +0000 http://www.bioradiations.com/?p=10104 Presented by: Brett Houser, Bio-Plex Multiplex Immunoassay Product Manager, Protein Quantitation Group

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Bio-Plex Manager Software is a powerful, intuitive tool used to generate and analyze xMap data. In this webinar you will:

  • Learn how to create an assay protocol
  • Learn how to acquire data
  • Understand basic software parameters
  • Learn about general functionality and navigation
  • Master optimization of standard curves and export data for further analysis
The post Bio-Plex Manager Software Overview first appeared on Bio-Radiations.]]> A 16-Color Flow Cytometry Panel to Monitor Checkpoint Inhibitor Expression and T-Cell Exhaustion Following Treatment with the PD1 Inhibitor Nivolumab https://www.bioradiations.com/a-16-color-flow-cytometry-panel-to-monitor-checkpoint-inhibitor-expression-and-t-cell-exhaustion-following-treatment-with-the-pd1-inhibitor-nivolumab/ Tue, 09 Oct 2018 23:15:05 +0000 http://www.bioradiations.com/?p=10232 Presented by: Angie Green, PhD, Senior Scientist

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We discuss using a multiparameter flow cytometry panel to monitor T-cell exhaustion states and the expression of checkpoint inhibitors. We used an in vitro model; however, the panel is designed to be compatible with clinical samples to monitor immunotherapy.

The post A 16-Color Flow Cytometry Panel to Monitor Checkpoint Inhibitor Expression and T-Cell Exhaustion Following Treatment with the PD1 Inhibitor Nivolumab first appeared on Bio-Radiations.]]> Large Biomolecule Purification with a New High-Performance Anion Exchange Resin https://www.bioradiations.com/large-biomolecule-purification-with-a-new-high-performance-anion-exchange-resin/ Tue, 09 Oct 2018 23:11:20 +0000 http://www.bioradiations.com/?p=10169 Presented by: Hana Kim, PhD, Global Product Manager, Process Chromatography
Jiali Liao, PhD, Principal Scientist, Process Chromatography

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Learn about Nuvia HP-Q Resin, a new rigid bead engineered with large pores and optimized surface extenders to deliver both high binding capacity and high recovery at high flow rates. It is ideal for large molecule purification, including plasma proteins, viruses, and VLPs.

The post Large Biomolecule Purification with a New High-Performance Anion Exchange Resin first appeared on Bio-Radiations.]]> A New Mixed-Mode Media for Downstream Purification https://www.bioradiations.com/a-new-mixed-mode-media-for-downstream-purification/ Tue, 09 Oct 2018 23:10:36 +0000 http://www.bioradiations.com/?p=10171 Presented by: Daniel Yoshikawa, PhD, Global Product Manager, Process Chromatography
Mark A. Snyder, PhD, R&D Applications Manager, Process Chromatography

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Watch this webinar to learn about a new mixed-mode chromatography media used to purify biomolecules at process scale. It delivers unique selectivity and robust, uniformly sized beads with excellent pressure-flow properties ideal for bioprocess applications.

The post A New Mixed-Mode Media for Downstream Purification first appeared on Bio-Radiations.]]> Traumatic Brain Injury — A Controversial and Deadly Epidemic https://www.bioradiations.com/traumatic-brain-injury-a-controversial-and-deadly-epidemic/ Tue, 11 Sep 2018 19:27:36 +0000 http://www.bioradiations.com/?p=10121 Ever wonder how football is linked to degenerative brain disease? Find out here. Also, learn about novel biomarkers for traumatic brain injury (TBI), the current state of TBI research, and promising new approaches for developing a robust diagnostic tool. Discover tests currently used to diagnose TBI and read about unusual techniques used to develop reliable biomarkers.

The post Traumatic Brain Injury — A Controversial and Deadly Epidemic first appeared on Bio-Radiations.]]> R-Loops and Their Key Role in Cancer Research https://www.bioradiations.com/r-loops-and-their-key-role-in-cancer-research/ Tue, 11 Sep 2018 19:11:59 +0000 http://www.bioradiations.com/?p=10116 R-loops are being investigated as potential therapeutic targets for cancer. Find out what they are, how they form, how their formation is controlled by our cells, and, above all, their potential as therapeutic targets.

The post R-Loops and Their Key Role in Cancer Research first appeared on Bio-Radiations.]]> Say Hello to Your Littlest Friends — the Microbiota https://www.bioradiations.com/say-hello-to-your-littlest-friends-the-microbiota/ Tue, 11 Sep 2018 19:04:03 +0000 http://www.bioradiations.com/?p=10110 How well do you understand your microbiota? Dr. Allyson Byrd of Genentech, the fourth guest on Brilliant Science, is a bioinformatician focusing on parsing all the data our microbiomes have to offer. Read about the value of analyzing microbiota and how Dr. Byrd hopes to leverage the results.

The post Say Hello to Your Littlest Friends — the Microbiota first appeared on Bio-Radiations.]]> To Boldly Flow: Gain Confidence to Start Flow Cytometry https://www.bioradiations.com/to-boldly-flow/ Tue, 11 Sep 2018 18:56:01 +0000 http://www.bioradiations.com/?p=10106 Presented by: Mike Blundell, PhD, Product Manager

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Flow cytometry is a powerful technique used widely in biological research, but if you are just beginning your flow journey, it can be hard to know where to start. In this webinar, you will:

  • Learn the important steps to obtaining better flow cytometry data
  • Get advice on how to apply this knowledge to improve your experiments
  • Find out how Bio-Rad’s panel builder tool can help you design your experiments, no matter how many colors you plan to use
The post To Boldly Flow: Gain Confidence to Start Flow Cytometry first appeared on Bio-Radiations.]]> Flow Cytometry — A Gateway for Discovering More about Small Vesicles https://www.bioradiations.com/flow-cytometry-a-gateway-for-discovering-more-about-small-vesicles/ Wed, 15 Aug 2018 18:42:00 +0000 http://www.bioradiations.com/?p=10035 Small vesicles were identified over 50 years ago but scientists are still trying to understand their biology and role in disease. These vesicles have become relevant for monitoring, diagnosing, and treating complex diseases such as cancer. Yet, because of their small size compared to cells, they require intricate technologies to truly understand their impact in research. Learn about the various technologies used to study small vesicles and discover the power of flow cytometry for learning more about these valuable vesicles.

The post Flow Cytometry — A Gateway for Discovering More about Small Vesicles first appeared on Bio-Radiations.]]> 10 Tips for Western Blot Detection of Proteins Present in Tissue Lysates https://www.bioradiations.com/10-tips-for-western-blot-detection-of-proteins-present-in-tissue-lysates/ Wed, 15 Aug 2018 18:33:06 +0000 http://www.bioradiations.com/?p=10029 Extracting proteins from tissue lysates for western blotting can be a challenge. From knowing how to process your tissue to verifying protein loading to including suitable controls, there is a lot to keep track of. The ten tips in this article can help you on your way to generating reproducible and reliable blots and do better western blotting overall.

The post 10 Tips for Western Blot Detection of Proteins Present in Tissue Lysates first appeared on Bio-Radiations.]]> Got Purification Troubles? https://www.bioradiations.com/got-purification-troubles/ Wed, 15 Aug 2018 14:49:39 +0000 http://www.bioradiations.com/?p=10041 Use our interactive infographic to find out how your resin-of-choice fares in on-column aggregate formation during monoclonal antibody purification. And find out what you can do to minimize these on-column aggregates.

The post Got Purification Troubles? first appeared on Bio-Radiations.]]> Defining the New Normal in Quantitative Western Blot Data https://www.bioradiations.com/defining-the-new-normal-in-quantitative-western-blot-data/ Wed, 15 Aug 2018 13:30:28 +0000 http://www.bioradiations.com/?p=8586 Normalization of western blot data is very crucial in quantitating proteins. Normalization of band intensity of proteins of interest with the band intensity of housekeeping proteins (HKP) is routinely done in labs. But more and more journals question the validity of using HKPs in normalization and are demanding more validations and tests. An alternative method is to use total protein normalization (TPN) for normalization. Explore the TPN option and see how you can get reliable western blot data easily.

The post Defining the New Normal in Quantitative Western Blot Data first appeared on Bio-Radiations.]]> Multiplex Cytokine Immunoassays — Minimizing Effort, Maximizing Results https://www.bioradiations.com/multiplex-cytokine-immunoassays-minimizing-effort-maximizing-results/ Tue, 10 Jul 2018 22:59:15 +0000 http://www.bioradiations.com/?p=9961 Since the first cytokine was identified over 60 years ago, a lot has been done to better understand these molecules. The use of multiplex immunoassays is one such way, though they aren’t considered quite as mainstream yet. Learn about how the SMARTS approach can be used to better align user experience to the performance characteristics of these multiplex assays and hopefully transition them to a more mainstream role in research.

The post Multiplex Cytokine Immunoassays — Minimizing Effort, Maximizing Results first appeared on Bio-Radiations.]]> A Resin Built Specifically for Large Biomolecule Purifications https://www.bioradiations.com/a-resin-built-specifically-for-large-biomolecule-purifications/ Tue, 10 Jul 2018 21:35:45 +0000 http://www.bioradiations.com/?p=9953 Nuvia HP-Q is a high-performance strong anion exchange resin designed for purification of large biomolecules. See how its optimized particle and pore size leads to high target recovery and high-throughput purifications.

The post A Resin Built Specifically for Large Biomolecule Purifications first appeared on Bio-Radiations.]]> Finding a Good Antibody: Part III – Publish Meaningful Data https://www.bioradiations.com/finding-a-good-antibody-part-iii-publish-meaningful-data/ Tue, 10 Jul 2018 21:30:26 +0000 http://www.bioradiations.com/?p=9945 In the first two articles in our Finding a Good Antibody series, you learned how to select the best antibodies and received tips on proper antibody validation. In this third and final article, see how you can use this information to publish meaningful data when using antibodies. And get some guidelines for antibody best practices.

The post Finding a Good Antibody:
Part III – Publish Meaningful Data first appeared on Bio-Radiations.]]> Finding New Horizons — What Possibilities Exist with Single-Cell Analysis? https://www.bioradiations.com/finding-new-horizons-what-possibilities-exist-with-single-cell-analysis/ Tue, 10 Jul 2018 21:17:10 +0000 http://www.bioradiations.com/?p=9940 Single-cell techniques offer exciting, new ways to understand personalized medicine and research. Developing these techniques at Bio-Rad is Dr. Kelly Kaihara, the third guest on Brilliant Science. Read about what makes single-cell analysis different and Kaihara’s perspective on its potential moving forward.

The post Finding New Horizons — What Possibilities Exist with Single-Cell Analysis? first appeared on Bio-Radiations.]]> Increase Yield and Reproducibility While Saving Time by Automating Recombinant Protein Purification https://www.bioradiations.com/increase-yield-and-reproducibility-while-saving-time-by-automating-recombinant-protein-purification/ Tue, 10 Jul 2018 21:00:48 +0000 http://www.bioradiations.com/?p=9937 Presented by: Philip Chapman, MSc, Senior Global Product Manager

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Learn how to quickly optimize conditions prior to automating a four-step purification of a histidine-tagged recombinant protein. Automating purification reduces a multi-day process into 12 hours while increasing yield and reproducibility.

The post Increase Yield and Reproducibility While Saving Time by Automating Recombinant Protein Purification first appeared on Bio-Radiations.]]> The Benefits of Flow Cytometry in Immuno-Oncology Research https://www.bioradiations.com/the-benefits-of-flow-cytometry-in-immuno-oncology-research/ Tue, 12 Jun 2018 23:00:16 +0000 http://www.bioradiations.com/?p=9864 Immuno-oncology, or cancer immunotherapy, research focuses heavily on the various types of cells and their behavior in cancer, so the use of flow cytometry to interrogate these cells is a no brainer. See how flow cytometry fits in this growing field of research and how it can help identify new targets and improve drug design to generate more effective and safer cancer therapeutics.

The post The Benefits of Flow Cytometry in Immuno-Oncology Research first appeared on Bio-Radiations.]]> Phagocytosis and Phasing Out Disease https://www.bioradiations.com/phagocytosis-and-phasing-out-disease/ Tue, 12 Jun 2018 22:55:49 +0000 http://www.bioradiations.com/?p=9832 Our immune systems could soon hold the key to rewriting disease and disorder treatments. Studying the inner workings of the immune system’s primary weapon, the macrophage, with the help of CRISPR is Dr. Adam Hoppe at SDSU. Read more about his exciting work and what he hopes to accomplish next.

The post Phagocytosis and Phasing Out Disease first appeared on Bio-Radiations.]]> Improve Your Protein Purification Process Economy with CHT Ceramic Hydroxyapatite XT Media https://www.bioradiations.com/improve-your-protein-purification-process-economy-with-cht-ceramic-hydroxyapatite-xt-media/ Tue, 12 Jun 2018 22:50:52 +0000 http://www.bioradiations.com/?p=9841 CHT Ceramic Hydroxyapatite XT Media is designed for repeated use and exceptional resolution. It combines exceptional functionality with a long column life to help achieve high productivity and improved process economics. See how CHT XT can improve your protein purification.

The post Improve Your Protein Purification Process Economy with CHT Ceramic Hydroxyapatite XT Media first appeared on Bio-Radiations.]]> Finding a Good Antibody:Part II — Antibody Validation https://www.bioradiations.com/finding-a-good-antibodypart-ii-antibody-validation/ Tue, 12 Jun 2018 22:41:34 +0000 http://www.bioradiations.com/?p=9851 Finding antibodies that work for your application is just the first step in finding a good antibody. Next is antibody validation — making sure that your antibody does what you think it is supposed to. In this second article in our Finding a Good Antibody series, learn how best to go about validating your antibodies.

The post Finding a Good Antibody:
Part II — Antibody Validation first appeared on Bio-Radiations.]]> When Small Matters — See How the ZE5 Cell Analyzer Can Help https://www.bioradiations.com/when-small-matters-see-how-the-ze5-cell-analyzer-can-help/ Tue, 12 Jun 2018 22:40:04 +0000 http://www.bioradiations.com/?p=9876 Presented by: Yasha Talaga, MSc, Applications and Collaborations Product Manager, Cell Biology

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In this webinar, Yasha Talaga will explain how specific methodologies and instrument setup on the ZE5 Cell Analyzer allow for reliable exosome detection data.

The post When Small Matters — See How the ZE5 Cell Analyzer Can Help first appeared on Bio-Radiations.]]> Best Practices for the Best Western Blots https://www.bioradiations.com/best-practices-for-the-best-western-blots/ Tue, 12 Jun 2018 22:30:38 +0000 http://www.bioradiations.com/?p=9873 Presented by: Paul Liu, PhD, Product Manager, Western Blot Reagents and Devices, Protein Quantitation Marketing
Date: Tuesday, July 10, 2018
Time: 10 AM, U.S. Pacific time

Western blotting is an essential and ubiquitous method for protein research. We will discuss method optimization, data analysis best practices, and new advancements in imaging and fluorescent detection reagents that make western blot data more reproducible and quantitative.

The post Best Practices for the Best Western Blots first appeared on Bio-Radiations.]]> The Potential for CRISPR Gene Editing in HIV https://www.bioradiations.com/the-potential-for-crispr-gene-editing-in-hiv/ Wed, 09 May 2018 19:29:53 +0000 http://www.bioradiations.com/?p=9772 The advent of CRISPR as a gene editing tool appears to have revolutionized drug discovery and development in a very short time. CRISPR-Cas9 technology is also being utilized to target HIV, to mutate or cut out the provirus. Read about what’s new in potential treatments for HIV and the challenges facing the industry.

The post The Potential for CRISPR Gene Editing in HIV first appeared on Bio-Radiations.]]> NYU Prof Harnesses CRISPR to End Muscular Dystrophy https://www.bioradiations.com/nyu-prof-harnesses-crispr-to-end-muscular-dystrophy/ Wed, 09 May 2018 19:27:45 +0000 http://www.bioradiations.com/?p=9775 On the scientific horizon are possibilities of curing genetic disorders and altering disease management — all because of CRISPR. Investigating exciting approaches to reversing the effects of muscular dystrophy with the help of CRISPR is Dr. Chengzu Long at NYU. Read more about his life, his work, and where he sees CRISPR going next.

The post NYU Prof Harnesses CRISPR to End Muscular Dystrophy first appeared on Bio-Radiations.]]> Finding a Good Antibody:Part I — Selecting the Best Antibodies https://www.bioradiations.com/finding-a-good-antibodypart-i-selecting-the-best-antibodies/ Wed, 09 May 2018 19:21:26 +0000 http://www.bioradiations.com/?p=9780 Even though antibodies are central to basic research as well as drug development and diagnostics, concerns with antibody validation remain high, and finding an antibody that works well for a specific application can be quite a challenge. Use these tips to select the best antibodies for your application.

The post Finding a Good Antibody:
Part I — Selecting the Best Antibodies first appeared on Bio-Radiations.]]> Cancer in the Age of Long Noncoding RNA Discovery https://www.bioradiations.com/cancer-in-the-age-of-long-noncoding-rna-discovery/ Wed, 11 Apr 2018 19:58:21 +0000 http://www.bioradiations.com/?p=9719 Cancer is one of the leading causes of death in the world and, due to an aging population, more and more people are diagnosed with cancer every year. Researchers are actively searching for treatment options in the form of potential biomarkers. Long noncoding RNAs (lncRNAs) have piqued the interest of researchers as a relatively new source of therapeutics. Learn more about lncRNAs and see how they can be used in cancer diagnosis and intervention.

The post Cancer in the Age of Long Noncoding RNA Discovery first appeared on Bio-Radiations.]]> Improve the Quality of Your Cell Analysis with the ZE5™ Cell Analyzer https://www.bioradiations.com/improve-the-quality-of-your-cell-analysis-with-the-ze5-cell-analyzer/ Wed, 11 Apr 2018 19:57:40 +0000 http://www.bioradiations.com/?p=9705 Cell analysis is a crucial first step in many experiments so a reliable, flexible, and quick cell analyzer that produces high-resolution, reproducible results is what every lab needs. See how the ZE5™ Cell Analyzer can help improve the quality of your cell analysis experiments, instill confidence in your results, and shorten the time to publish.

The post Improve the Quality of Your Cell Analysis with the ZE5™ Cell Analyzer first appeared on Bio-Radiations.]]> The How and Why of Normalizing Your Western Blots https://www.bioradiations.com/the-how-and-why-of-normalizing-your-western-blots/ Wed, 14 Mar 2018 16:59:06 +0000 http://www.bioradiations.com/?p=9661 Introducing data normalization into your western blotting workflow may sound unnecessary but it has proven to be a great way to ensure great results. Follow along as we break down some of the main takeaways from the first chapter in Dr. Oh’s popular wester blotting webinar series, including a unique method to ease normalization woes and worries.

The post The How and Why of Normalizing Your Western Blots first appeared on Bio-Radiations.]]> High-Throughput Single-Cell RNA-Seq of Large Cells and Nuclei https://www.bioradiations.com/high-throughput-single-cell-rna-seq-of-large-cells-and-nuclei/ Wed, 14 Mar 2018 16:41:55 +0000 http://www.bioradiations.com/?p=9647 mRNA-FISH, single-cell RT-qPCR, and single-cell RNA sequencing (RNA-Seq) have emerged as popular single-cell transcriptomics technologies. See how the Illumina® Bio-Rad® Single-Cell Sequencing Solution overcomes many of the challenges of currently available platforms by allowing high-throughput and scalable sequencing of even the most challenging sample types, including large cells and small species such as nuclei.

The post High-Throughput Single-Cell RNA-Seq of Large Cells and Nuclei first appeared on Bio-Radiations.]]> Bio-Plex® Multiplex Immunoassay Technology Overview and Setting Up to Run an Assay https://www.bioradiations.com/bio-plex-multiplex-immunoassay-technology-overview-and-setting-up-to-run-an-assay/ Wed, 14 Mar 2018 16:30:15 +0000 http://www.bioradiations.com/?p=9678 Presented by: Brett Houser
Date: Tuesday, March 20, 2018
Time: 9 AM, U.S. Pacific time

Multiplex immunoassays are an extremely powerful tool for protein detection and quantitation. In this webinar, get a general overview of multiplex immunoassay technology and tips on setting up experiments to optimize your results.

The post Bio-Plex® Multiplex Immunoassay Technology Overview and Setting Up to Run an Assay first appeared on Bio-Radiations.]]> The Ups and Downs of Biosimilar Development https://www.bioradiations.com/podcast-the-ups-and-downs-of-biosimilar-development/ Wed, 14 Mar 2018 16:23:40 +0000 http://www.bioradiations.com/?p=9642 In this podcast, biosimilar key opinion leader Dr. Paul Rhyne provides an overview of the current state of biosimilars and their global impact. Leveraging his 20+ years of experience in developing biologics, he discusses how cost and patient access play a big role in this new field of research.

The post The Ups and Downs of Biosimilar Development first appeared on Bio-Radiations.]]> Preamplification and How it Can Be Used to Maximize qPCR Data Generation from Limited Samples https://www.bioradiations.com/preamplification-and-how-it-can-be-used-to-maximize-qpcr-data-generation-from-limited-samples/ Wed, 14 Feb 2018 00:15:14 +0000 http://www.bioradiations.com/?p=9608 Incorporating preamplification into your qPCR experiments may seem daunting, but it is a powerful way to analyze targets from limited samples. The tips in this article will help you understand how preamplification can enhance your qPCR experiments and the considerations and implications for incorporating it into your workflow.

The post Preamplification and How it Can Be Used to Maximize qPCR Data Generation from Limited Samples first appeared on Bio-Radiations.]]> Biosimilars: Providing More Treatment Options https://www.bioradiations.com/biosimilars-providing-more-treatment-options/ Wed, 14 Feb 2018 00:10:47 +0000 http://www.bioradiations.com/?p=9626 It is hoped that the development of biosimilars will lead to more accessible and cost-effective treatment options. Here we explore the current opportunities for biosimilars in rheumatoid arthritis and discuss the opportunities and concerns tied to their use.

The post Biosimilars: Providing More Treatment Options first appeared on Bio-Radiations.]]> Introducing the NGC™ Fraction Collector for Discovery to Process-Scale Protein Purification https://www.bioradiations.com/introducing-the-ngc-fraction-collector-for-discovery-to-process-scale-protein-purification/ Tue, 13 Feb 2018 23:59:43 +0000 http://www.bioradiations.com/?p=9603 The newly launched NGC™ Fraction Collector, together with ChromLab™ 5.0 Software, have enhanced the functionality of the NGC Chromatography System to provide the flexibility and collection capacity needed for any application, from discovery to scale up. See how the NGC System can help you improve your protein purification process.

The post Introducing the NGC™ Fraction Collector for Discovery to Process-Scale Protein Purification first appeared on Bio-Radiations.]]> Generating Semi-Quantitative Western Blot Data Using Bio-Rad’s Image Lab™ 6.0 Software https://www.bioradiations.com/generating-semi-quantitative-western-blot-data-using-bio-rads-image-lab-6-0-software/ Tue, 13 Feb 2018 23:24:27 +0000 http://www.bioradiations.com/?p=9599 Presented by: Kenneth J. Oh, PhD, Applications, Collaborations, and New Technology Manager, Protein Quantitation Marketing Group, Bio-Rad Laboratories, Inc.
Date: Tuesday, March 6, 2018

Western blotting is a popular tool for protein quantitation, and the advent of digital imaging has greatly increased the sensitivity, dynamic range, and quantitation limits of this technique. In the second webinar in this series, get step-to-step instructions for normalizing western blot data using Image Lab 6.0 Software.

The post Generating Semi-Quantitative Western Blot Data Using Bio-Rad’s Image Lab™ 6.0 Software first appeared on Bio-Radiations.]]> What’s New in U.S. Biosimilar-Land? https://www.bioradiations.com/whats-new-in-u-s-biosimilar-land/ Tue, 09 Jan 2018 23:55:17 +0000 http://www.bioradiations.com/?p=9583 Biosimilars are gaining in popularity and estimates predict that the U.S. could save between $40–250 billion in healthcare costs over the next decade by switching from biologics to biosimilars. See how biosimilars have gained in popularity recently, the challenges the U.S. faces in developing them, and what the future holds.

The post What’s New in U.S. Biosimilar-Land? first appeared on Bio-Radiations.]]> Minimizing On-Column Monoclonal Antibody Aggregate Formation https://www.bioradiations.com/minimizing-on-column-monoclonal-antibody-aggregate-formation/ Tue, 09 Jan 2018 23:35:24 +0000 http://www.bioradiations.com/?p=9576 The formation of aggregates is a common but highly undesirable occurrence during monoclonal antibody purification. See how on-column aggregation is dependent on the type of resin used and how one particular high-resolution cation exchange resin allows for the lowest proportion of aggregate formation.

The post Minimizing On-Column Monoclonal Antibody Aggregate Formation first appeared on Bio-Radiations.]]> Identifying Predictive Biomarkers for Successful Immunotherapy Treatments https://www.bioradiations.com/identifying-predictive-biomarkers-for-successful-immunotherapy-treatments/ Tue, 09 Jan 2018 23:08:16 +0000 http://www.bioradiations.com/?p=9563 Find out how the S3e™ Cell Sorter can help scientists discover new biomarkers that have the potential to become new therapies for cancer and other diseases.

The post Identifying Predictive Biomarkers for Successful Immunotherapy Treatments first appeared on Bio-Radiations.]]> Year in Review: What We Learned in 2017 https://www.bioradiations.com/year-in-review-what-we-learned-in-2017/ Tue, 12 Dec 2017 17:48:50 +0000 http://www.bioradiations.com/?p=9551 In 2017, Bioradiations published a wide variety of general interest articles, tips, podcasts, and application notes. Revisit the technological advancements and growth in technology that researchers experienced this year. Here are the highlights of what we learned this year.

The post Year in Review: What We Learned in 2017 first appeared on Bio-Radiations.]]> Top Ten Videos of 2017 https://www.bioradiations.com/top-ten-videos-of-2017/ Tue, 12 Dec 2017 17:48:19 +0000 http://www.bioradiations.com/?p=9549 Explore the most watched Bio-Rad videos in 2017. From understanding CRISPR gene editing to generating better qPCR data to selecting antibodies for fluorescent western blotting, these videos highlight how we continually strive to help our customers do better science.

The post Top Ten Videos of 2017 first appeared on Bio-Radiations.]]> Top Five Podcasts of 2017 https://www.bioradiations.com/top-five-podcasts-of-2017/ Tue, 12 Dec 2017 17:47:45 +0000 http://www.bioradiations.com/?p=9524 2017 saw the launch of Bioradiations’ first-ever podcast! Here you can listen to the top five podcasts we published this year, and learn about a variety of topics from CRISPR gene editing to what biomarkers and biosimilars are.

The post Top Five Podcasts of 2017 first appeared on Bio-Radiations.]]> Cell Sorting and Its Significance in Stem Cell Research https://www.bioradiations.com/cell-sorting-and-its-significance-in-stem-cell-research/ Thu, 16 Nov 2017 16:49:52 +0000 http://www.bioradiations.com/?p=9514 Stem cells possess the remarkable potential to self-renew and to differentiate into many different types of cells. Due to their unique ability to regenerate, they are being used in regenerative medicine and offer great hope for treating diseases such as diabetes and heart disease. However, since stem cells represent a rare population of cells, they are not always easy to study. See how the simplicity, efficiency, and flexibility of cell sorting is making it possible to isolate pure populations of stem cells for downstream analyses.

The post Cell Sorting and Its Significance in Stem Cell Research first appeared on Bio-Radiations.]]> Advances in Immunotherapy for Cancer https://www.bioradiations.com/advances-in-immunotherapy-for-cancer/ Thu, 16 Nov 2017 16:47:02 +0000 http://www.bioradiations.com/?p=9510 Cancer kills eight million people annually worldwide; this number will increase to 13 million by 2020. Lung, breast, colorectal, and prostate cancer are the four most commonly diagnosed cancers. Meaningful developments in immuno-oncology, or cancer immunotherapy, treatments are important now more than ever. Learn about some of the newer immunotherapies, including BiTE Antibodies and vaccines, and the companies developing them.

The post Advances in Immunotherapy for Cancer first appeared on Bio-Radiations.]]> Apolipoproteins as Biomarkers in Heart Disease and Beyond https://www.bioradiations.com/apolipoproteins-as-biomarkers-in-heart-disease-and-beyond/ Thu, 16 Nov 2017 16:43:44 +0000 http://www.bioradiations.com/?p=9498 Apolipoproteins bind to lipids to form lipoproteins, which transport lipids throughout the lymphatic and circulatory systems. In addition to cardiovascular disease, apolipoproteins are now being linked to other diseases such as cancer, autoimmunity, and neurodegeneration. Learn more about the different types of apolipoproteins and see how they serve as biomarkers for these diseases.

The post Apolipoproteins as Biomarkers in Heart Disease and Beyond first appeared on Bio-Radiations.]]> Western Blot Normalization Methods https://www.bioradiations.com/western-blot-normalization-methods/ Thu, 12 Oct 2017 00:16:07 +0000 http://www.bioradiations.com/?p=9340 Presented by: Kenneth J. Oh, PhD, Applications, Collaborations, and New Technology Manager, Protein Quantitation Marketing Group, Bio-Rad Laboratories, Inc.
Date: Tuesday, October 24, 2017

Western blotting continues to be a ubiquitous tool for the identification and quantitation of protein(s) of interest. The first webinar in this series will focus on common normalization techniques for quantitative western blotting and discuss important considerations when using these techniques.

The post Western Blot Normalization Methods first appeared on Bio-Radiations.]]> Immunotherapy: The Next Frontier for Cancer https://www.bioradiations.com/immunotherapy-the-next-frontier-for-cancer/ Thu, 12 Oct 2017 00:06:31 +0000 http://www.bioradiations.com/?p=9332 Immunotherapy is not a new concept; it has, in fact, been around for more than 125 years. However, recent scientific advances have led to the emergence and rapid evolution of the field, especially immuno-oncology. Get an introduction to this exciting emerging field, learn about the different personalized therapies currently available, and see what the future of cancer immunotherapy looks like.

The post Immunotherapy: The Next Frontier for Cancer first appeared on Bio-Radiations.]]> Practical Experimental Design for Mixed-Mode Resins https://www.bioradiations.com/practical-experimental-design-for-mixed-mode-resins/ Wed, 11 Oct 2017 23:53:03 +0000 http://www.bioradiations.com/?p=9326 Could you be missing opportunities to make the most of your time spent on protein chromatography experiments? Learn more about how Bio-Rad uses design of experiments (DoE) and our mixed-mode resin, Nuvia™ cPrime™, to optimize process-scale protein purification.

The post Practical Experimental Design for Mixed-Mode Resins first appeared on Bio-Radiations.]]> Tips to Make Fluorophore Picking Easier https://www.bioradiations.com/tips-to-make-fluorophore-picking-easier/ Wed, 11 Oct 2017 23:40:49 +0000 http://www.bioradiations.com/?p=9314 Fluorescence is a property of photo-excitable molecules that has been exploited to develop dyes, or fluorophores, that are used in a variety of experimental procedures, including western blotting. Learn more about the science behind fluorescent dyes and get some helpful tips on how to pick the right fluorophores for your experimental needs.

The post Tips to Make Fluorophore Picking Easier first appeared on Bio-Radiations.]]> Speed Time to Results by Incorporating Flow Cytometry into the CRISPR‑Cas9 Gene Editing Workflow https://www.bioradiations.com/speed-time-to-results-by-incorporating-flow-cytometry-into-the-crisprcas-9-gene-editing-workflow/ Tue, 12 Sep 2017 22:38:32 +0000 http://www.bioradiations.com/?p=9278 The power to edit a gene is the power to change its function, and with it the biology of a cell. From generating novel cell lines and better animal models for the discovery and preclinical phases of therapeutic research to actually creating a therapeutic itself, CRISPR gene editing is allowing science to advance rapidly. See how enrichment and cell sorting can help generate edited cells faster and more reliably.

The post Speed Time to Results by Incorporating Flow Cytometry into the CRISPR‑Cas9 Gene Editing Workflow first appeared on Bio-Radiations.]]> Discover the Technology behind Bio-Rad’s Custom Recombinant Antibodies https://www.bioradiations.com/discover-the-technology-behind-bio-rads-custom-recombinant-antibodies/ Tue, 12 Sep 2017 22:38:20 +0000 http://www.bioradiations.com/?p=9274 See inside Bio-Rad's custom antibody facility and learn how specific antibody requirements can become a reality through the use of Human Combinatorial Antibody Library (HuCAL®) technology for specialized custom antibodies.

The post Discover the Technology behind Bio-Rad’s Custom Recombinant Antibodies first appeared on Bio-Radiations.]]> The Importance of In Vitro Assays in the Development of Biosimilars https://www.bioradiations.com/podcast-the-importance-of-in-vitro-assays-in-the-development-of-biosimilars/ Tue, 12 Sep 2017 22:38:12 +0000 http://www.bioradiations.com/?p=9268 In this podcast, biosimilar expert, Amanda Turner, provides an in-depth explanation of the various in vitro assays used in biosimilar development and the importance of choosing the right assay type and components to achieve success.

The post The Importance of In Vitro Assays in the Development of Biosimilars first appeared on Bio-Radiations.]]> Understanding Biomarkers for Better Drug Discovery and Development https://www.bioradiations.com/understanding-biomarkers-for-better-drug-discovery-and-development/ Wed, 09 Aug 2017 19:03:58 +0000 http://www.bioradiations.com/?p=9251 Biomarker intelligence comes from understanding the biomarker landscape, that is, the characteristics and features of biomarkers, and disease pathways. This understanding, in turn, helps to recognize the utility of biomarkers. In this study, we partnered with Amplion Inc., to investigate biomarker usage in 24 immunotherapy companies. We found the most popular biomarkers and learnt how they are being used by each company.

The post Understanding Biomarkers for Better Drug Discovery and Development first appeared on Bio-Radiations.]]> An IMAC Resin for a Highly Productive Histidine-Tagged Protein Purification Process https://www.bioradiations.com/an-imac-resin-for-a-highly-productive-histidine-tagged-protein-purification-process/ Wed, 09 Aug 2017 18:30:59 +0000 http://www.bioradiations.com/?p=9239 One of the advancements in protein purification has been to tag the protein of interest with a short sequence that can bind to an immobilized matrix and aid purification. Bio-Rad’s new IMAC resin offers a host of advantages for the purification of histidine-tagged proteins. Learn more about this resin and see how it can enhance the efficiency of your protein purification workflow.

The post An IMAC Resin for a Highly Productive Histidine-Tagged Protein Purification Process first appeared on Bio-Radiations.]]> BrdU in Adult Neurogenesis Research https://www.bioradiations.com/podcast-brdu-in-adult-neurogenesis-research/ Wed, 09 Aug 2017 17:55:25 +0000 http://www.bioradiations.com/?p=9233 In this podcast, you will learn about the history of bromodeoxyuridine (BrdU) and how it was established as a popular tool for neurogenesis research. Gain insight into the latest BrdU-related findings and get five practical tips for controlling your BrdU labeling experiments.

The post BrdU in Adult Neurogenesis Research first appeared on Bio-Radiations.]]> Biosimilars. Are They Ready for Primetime? https://www.bioradiations.com/biosimilars-are-they-ready-for-primetime/ Wed, 12 Jul 2017 21:34:47 +0000 http://www.bioradiations.com/?p=9222 Is the hype surrounding biosimilars real or simply a hopeful view on an exceedingly expensive drug market? Read this article to find out more about biosimilars and understand the challenges facing the biosimilars industry.

The post Biosimilars. Are They Ready for Primetime? first appeared on Bio-Radiations.]]> Understanding the Eukaryotic Cell Cycle https://www.bioradiations.com/understanding-the-eukaryotic-cell-cycle/ Wed, 12 Jul 2017 21:20:57 +0000 http://www.bioradiations.com/?p=9218 The eukaryotic cell cycle is an evolutionarily conserved process that results in the replication of cells. Read this mini review to better understand the eukaryotic cell cycle and get an overview of common molecular techniques used to evaluate proliferating cells.

The post Understanding the Eukaryotic Cell Cycle first appeared on Bio-Radiations.]]> Why Biomarkers Are Important for Clinical Trial Success https://www.bioradiations.com/podcast-why-biomarkers-are-important-for-clinical-trial-success/ Wed, 12 Jul 2017 17:29:13 +0000 http://www.bioradiations.com/?p=9212 In this podcast, learn more about biomarkers and their importance in clinical trials and how data-rich tools are being used to collect and curate the vast amounts of publicly available information about biomarkers.

The post Why Biomarkers Are Important for Clinical Trial Success first appeared on Bio-Radiations.]]> Modern Improvements to Classic Technologies: Innovating Electrophoresis and Chromatography Upstream of Protein Characterization to Speed Up Research https://www.bioradiations.com/modern-improvements-to-classic-technologies-innovating-electrophoresis-and-chromatography-upstream-of-protein-characterization-to-speed-up-research/ Thu, 15 Jun 2017 21:07:15 +0000 http://www.bioradiations.com/?p=9186 When testing new drug therapies, it is essential to know the identity of the protein being tested. Chromatography is a commonly used technique to separate complex biological mixtures and isolate proteins of interest prior to downstream characterization. Learn more about how automating chromatography and stain-free technology are helping to save time and effort in protein purification.

The post Modern Improvements to Classic Technologies: Innovating Electrophoresis and Chromatography Upstream of Protein Characterization to Speed Up Research first appeared on Bio-Radiations.]]> Get More Answers from Your Mouse with the Bio-Plex Pro™ Mouse Chemokine Panel https://www.bioradiations.com/get-more-answers-from-your-mouse-with-the-bio-plex-pro-mouse-chemokine-panel/ Thu, 15 Jun 2017 20:59:30 +0000 http://www.bioradiations.com/?p=9180 Biomarker screening can sometimes be like working in a black box. While traditional research tools focus on one protein at a time, Bio-Rad’s new Bio-Plex Pro™ Mouse Chemokine Panel can simultaneously interrogate 33 chemokines and cytokines in a single well. See how you can get more answers with this panel.

The post Get More Answers from Your Mouse with the Bio-Plex Pro™ Mouse Chemokine Panel first appeared on Bio-Radiations.]]> CRISPR Gene Editing and Its Effect on Research and Development https://www.bioradiations.com/podcast-crispr-gene-editing-and-its-effect-on-research-and-development/ Thu, 15 Jun 2017 20:52:10 +0000 http://www.bioradiations.com/?p=9173 Our resident gene editing expert, Dr. Yann Jouvenot, provides an overview of CRISPR gene editing and how it is affecting how research and development are being performed in the Biotech and Pharma industries.

The post CRISPR Gene Editing and Its Effect on Research and Development first appeared on Bio-Radiations.]]> Identify Major Immune Cell Types by Their CD Marker Expression https://www.bioradiations.com/identify-major-immune-cell-types-by-their-cd-marker-expression/ Thu, 15 Jun 2017 20:39:31 +0000 http://www.bioradiations.com/?p=9168 Our new immune cell lineage and marker database provides an interactive way to help you easily examine detailed marker data, view immune cell marker expression, and find the antibody to your marker. Learn more and try out the interactive tool for yourself.

The post Identify Major Immune Cell Types by Their CD Marker Expression first appeared on Bio-Radiations.]]> What Pharma Is Doing to Triple Clinical Trial Successes https://www.bioradiations.com/what-pharma-is-doing-to-triple-clinical-trial-successes/ Wed, 10 May 2017 01:00:33 +0000 http://www.bioradiations.com/?p=9147 The typical success rate of a clinical trial is less than ten percent. Pharmaceutical companies are using a variety of methods to save time and reduce costs to get drugs to market faster. Biomarker screening is proving to be an effective way to improve the drug discovery and development process. See one of the ways Pharma companies can identify the most relevant biomarkers.

The post What Pharma Is Doing to Triple Clinical Trial Successes first appeared on Bio-Radiations.]]> Validating Antibodies — the Good, the Bad, and the Necessary https://www.bioradiations.com/validating-antibodies-the-good-the-bad-and-the-necessary/ Wed, 10 May 2017 00:54:28 +0000 http://www.bioradiations.com/?p=9143 Despite the fact that antibodies are one of the most frequently used tools in life science and clinical research, there are no universally accepted guidelines to ensure that they have been properly validated. Learn about the issues facing researchers and what antibody suppliers and researchers can do to ensure that the research being carried out is trustworthy and is helping to improve overall data quality.

The post Validating Antibodies — the Good, the Bad, and the Necessary first appeared on Bio-Radiations.]]> Overview of the Four Main Gene Editing Techniques Used in Drug Discovery https://www.bioradiations.com/podcast-overview-of-the-four-main-gene-editing-techniques-used-in-drug-discovery/ Wed, 10 May 2017 00:49:55 +0000 http://www.bioradiations.com/?p=9135 Our resident gene editing expert, Dr. Yann Jounenvat, provides an overview of the four main gene editing techniques that are being used in life science research today. Learn about the pros and cons of each of method and where they are best applied.

The post Overview of the Four Main Gene Editing Techniques Used in Drug Discovery first appeared on Bio-Radiations.]]> Key Steps to Ensure Optimized and Reproducible qPCR Experiments https://www.bioradiations.com/key-steps-to-ensure-optimized-and-reproducible-qpcr-experiments/ Fri, 14 Apr 2017 00:37:07 +0000 http://www.bioradiations.com/?p=9108 Real-time PCR is a common and easy-to-adopt technology. By keeping a few simple steps in mind as you design and run your experiments you can ensure high-quality, reproducible data.

The post Key Steps to Ensure Optimized and Reproducible qPCR Experiments first appeared on Bio-Radiations.]]> Rapid, Sensitive Cancer Biomarker Screening in cfDNA https://www.bioradiations.com/rapid-sensitive-cancer-biomarker-screening-in-cfdna/ Fri, 14 Apr 2017 00:32:06 +0000 http://www.bioradiations.com/?p=9094 Disease-associated mutations can be prognostic of outcome and inform treatment choices. However, traditional tissue biopsies limit the ability to implement personalized treatments. Read about how multiplex digital PCR can enable the tracking of treatment efficacy using liquid biopsies.

The post Rapid, Sensitive Cancer Biomarker Screening in cfDNA first appeared on Bio-Radiations.]]> Erasing Disease: How Gene Editing is Changing Genetic Therapies https://www.bioradiations.com/erasing-disease-how-gene-editing-is-changing-genetic-therapies/ Wed, 15 Mar 2017 23:10:37 +0000 http://www.bioradiations.com/?p=9070 Gene augmentation and antisense therapies treat disease by providing a functional copy of faulty gene or repressing its expression. Find out how gene editing tools, like CRISPR, promise a more permanent cure.

The post Erasing Disease: How Gene Editing is Changing Genetic Therapies first appeared on Bio-Radiations.]]> Assessing Cell Health: Necroptosis https://www.bioradiations.com/assessing-cell-health-necroptosis/ Wed, 15 Mar 2017 23:05:29 +0000 http://www.bioradiations.com/?p=9056 In this fourth and final part of the series on assessing the health of your cells, we review the different stages of necroptosis, see how it differs from apoptosis, and learn about the various methods of assessing cellular necroptosis.

The post Assessing Cell Health: Necroptosis first appeared on Bio-Radiations.]]> Investigating Immune Response Profiles Using Bio-Plex Pro™ Multiplex Assays https://www.bioradiations.com/investigating-immune-response-profiles-using-bio-plex-pro-multiplex-assays/ Wed, 15 Mar 2017 23:01:21 +0000 http://www.bioradiations.com/?p=9050 See how one study used Bio-Rad’s Bio-Plex Pro Human Cytokine 27-Plex Assay to investigate immune responses to an anti-viral compound in an effort to better understand vaccine efficacy.

The post Investigating Immune Response Profiles Using Bio-Plex Pro™ Multiplex Assays first appeared on Bio-Radiations.]]> Fluorescent Western Blotting: Helpful Tips for Optimizing Your Protocol https://www.bioradiations.com/fluorescent-western-blotting-helpful-tips-for-optimizing-your-protocol/ Wed, 15 Feb 2017 23:04:12 +0000 http://www.bioradiations.com/?p=9016 Western blotting using fluorescently tagged detection antibodies is a technique that is gaining in popularity. However, adopting a new technique can be challenging. Use the tips provided in this article to get started and see how fluorescent western blotting can enhance your scientific research.

The post Fluorescent Western Blotting: Helpful Tips for Optimizing Your Protocol first appeared on Bio-Radiations.]]> The ZE5™ Cell Analyzer: Designed by Users for Users https://www.bioradiations.com/the-ze5-cell-analyzer-designed-by-users-for-users/ Wed, 15 Feb 2017 23:00:07 +0000 http://www.bioradiations.com/?p=9009 Bio-Rad’s new ZE5 Cell Analyzer is a game-changing cell analyzer that was designed with direct input from scientists and flow core labs. Karen Helm runs the flow core facility at the University of Colorado Cancer Center and was one of the experts consulted. See how ZE5 is helping her flow core users do better science.

The post The ZE5™ Cell Analyzer: Designed by Users for Users first appeared on Bio-Radiations.]]> Characterization of Critical Reagents for Ligand Binding Assays https://www.bioradiations.com/characterization-of-critical-reagents-for-ligand-binding-assays/ Wed, 15 Feb 2017 22:29:13 +0000 http://www.bioradiations.com/?p=8981 Ligand binding assays are utilized throughout the large-molecule drug development process. However, their robustness, accuracy, and reproducibility depend on the quality of critical reagents. See how you can ensure their consistent characterization, particularly during early drug development stages.

The post Characterization of Critical Reagents for Ligand Binding Assays first appeared on Bio-Radiations.]]> The Fluorescent Way: Why Start Fluorescent Western Blotting Now? https://www.bioradiations.com/the-fluorescent-way-why-start-fluorescent-western-blotting-now/ Fri, 13 Jan 2017 00:56:06 +0000 http://www.bioradiations.com/?p=8952 Find out why fluorescent western blotting is gaining in popularity and how it compares to more widely used methods, such as chemiluminescence. From easier multiplexing to brighter fluorophores to easier method development, learn about the many advantages of fluorescent western blotting.

The post The Fluorescent Way: Why Start Fluorescent Western Blotting Now? first appeared on Bio-Radiations.]]> Multiplex Immunoassays: Five Tips for Optimizing Your Experiments https://www.bioradiations.com/multiplex-immunoassays-five-tips-for-optimizing-your-experiments/ Fri, 13 Jan 2017 00:51:22 +0000 http://www.bioradiations.com/?p=8942 Follow the five tips in this article before embarking on your multiplex immunoassay journey to generate accurate, efficient, and biologically relevant results. From help designing experiments to acquiring accurate data, this article has it all.

The post Multiplex Immunoassays: Five Tips for Optimizing Your Experiments first appeared on Bio-Radiations.]]> CRISPR: Changing the Pace of BioPharma R&D https://www.bioradiations.com/crispr-changing-the-pace-of-biopharma-rd/ Fri, 16 Dec 2016 17:54:51 +0000 http://www.bioradiations.com/?p=8929 Better model systems and faster, more comprehensive screens are only two of the many ways in which CRISPR is changing the pace of discovery. See how CRISPR is transforming research and development by providing us with tools that were once unimaginable.

The post CRISPR: Changing the Pace of BioPharma R&D first appeared on Bio-Radiations.]]> Year in Review: Top Ten Articles of 2016 https://www.bioradiations.com/year-in-review-top-ten-articles-of-2016/ Fri, 16 Dec 2016 17:48:20 +0000 http://www.bioradiations.com/?p=8924 In 2016, we published a wide variety of general interest articles, customer testimonials, tips, protocols, videos, product highlight articles, and technical and application notes. Here are the ten articles you found the most interesting in 2016.

The post Year in Review: Top Ten Articles of 2016 first appeared on Bio-Radiations.]]> The Making of Recombinant Anti-Idiotypic Antibodies For High Performance in Bioanalytical Assays https://www.bioradiations.com/the-making-of-recombinant-anti-idiotypic-antibodies-for-high-performance-in-bioanalytical-assays/ Wed, 30 Nov 2016 17:53:31 +0000 http://www.bioradiations.com/?p=8912 Discover how Bio-Rad develops highly specific, high-affinity recombinant monoclonal anti-idiotypic antibodies ideal for use in PK and ADA assays.

The post The Making of Recombinant Anti-Idiotypic Antibodies For High Performance in Bioanalytical Assays first appeared on Bio-Radiations.]]> Digital PCR: Helpful Tips When Using Droplet Partitioning Technology https://www.bioradiations.com/digital-pcr-helpful-tips-when-using-droplet-partitioning-technology/ Thu, 10 Nov 2016 00:43:28 +0000 http://www.bioradiations.com/?p=8907 Digital PCR offers a highly sensitive alternative to conventional qPCR, enabling absolute quantification of nucleic acids and rare allele detection. The tips in this article help you set up and optimize your digital PCR experiments when using droplet partitioning technology.

The post Digital PCR: Helpful Tips When Using Droplet Partitioning Technology first appeared on Bio-Radiations.]]> Ten Tips for Improving Western Blot Detection of Immunoprecipitation Samples https://www.bioradiations.com/ten-tips-for-improving-western-blot-detection-of-immunoprecipitation-samples/ Thu, 10 Nov 2016 00:38:14 +0000 http://www.bioradiations.com/?p=8904 Immunoprecipitation (IP) experiments and the detection of IP samples by western blotting can be quite challenging at times. The tips in this article help you set up and optimize your overall IP experimenta design.

The post Ten Tips for Improving Western Blot Detection of Immunoprecipitation Samples first appeared on Bio-Radiations.]]> TGX Stain-Free™ FastCast™ Acrylamide Gel Casting https://www.bioradiations.com/tgx-stain-free-fastcast-acrylamide-gel-casting/ Thu, 10 Nov 2016 00:35:30 +0000 http://www.bioradiations.com/?p=8901 This short video demonstrates how you can access the unique benefits of Bio-Rad's proprietary TGX Stain-Free Gel chemistry by hand casting your own TGX Stain-Free Gels in just a few quick and easy steps.

The post TGX Stain-Free™ FastCast™ Acrylamide Gel Casting first appeared on Bio-Radiations.]]> Parallel Analysis of RNA and Protein Expression Using SingleShot™ Cell Culture Lysates https://www.bioradiations.com/parallel-analysis-of-rna-and-protein-expression-using-singleshot-cell-culture-lysates/ Thu, 10 Nov 2016 00:30:00 +0000 http://www.bioradiations.com/?p=8883 Comprehensive and effective gene expression studies evaluate both transcriptional and translational regulation. A streamlined protocol was developed for the parallel analysis of mRNA and protein levels using a single lysate produced from cultured cells.

The post Parallel Analysis of RNA and Protein Expression Using SingleShot™ Cell Culture Lysates first appeared on Bio-Radiations.]]> Introduction to Algae Beads, Photosynthesis, and Cellular Respiration https://www.bioradiations.com/introduction-to-algae-beads-photosynthesis-and-cellular-respiration/ Thu, 10 Nov 2016 00:22:26 +0000 http://www.bioradiations.com/?p=8880 Introduce students (and teachers) to the concepts of photosynthesis and cellular respiration in the context of how these processes evolved and how they affect our daily lives.

The post Introduction to Algae Beads, Photosynthesis, and Cellular Respiration first appeared on Bio-Radiations.]]> Take Control of Your Flow Cytometry https://www.bioradiations.com/take-control-of-your-flow-cytometry/ Thu, 03 Nov 2016 16:46:27 +0000 http://www.bioradiations.com/?p=8870 Recommended for researchers with limited experience of flow cytometry.

The post Take Control of Your Flow Cytometry first appeared on Bio-Radiations.]]> Assessing Cell Health: Autophagy https://www.bioradiations.com/assessing-cell-health-autophagy/ Thu, 13 Oct 2016 17:40:47 +0000 http://www.bioradiations.com/?p=8859 Part III of our cell health series describes the three main autophagy mechanisms and reviews the various methods for assessing macroautophagy and autophagic flux in cells.

The post Assessing Cell Health: Autophagy first appeared on Bio-Radiations.]]> From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification https://www.bioradiations.com/from-optimization-to-automation-multidimensional-multi-d-histidine-tag-protein-purification/ Thu, 13 Oct 2016 17:39:47 +0000 http://www.bioradiations.com/?p=8840 A well-designed multidimensional (Multi-D) chromatography process, which utilizes optimized individual column methods, can help automate multistep protein purification processes. This can help you save time and increase productivity. See how you can use Multi-D chromatography to expedite the purification of polyhistidine-tagged proteins when using a typical capture, intermediate, and polish purification workflow.

The post From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification first appeared on Bio-Radiations.]]> Meet the Brightest ECL Substrate Around — Clarity Max™ Western ECL Substrate https://www.bioradiations.com/meet-the-brightest-ecl-substrate-around-clarity-max-western-ecl-substrate/ Thu, 13 Oct 2016 17:38:47 +0000 http://www.bioradiations.com/?p=8837 See how our new high-sensitivity western ECL substrate, Clarity Max, can help you achieve greater western blotting success.

The post Meet the Brightest ECL Substrate Around — Clarity Max™ Western ECL Substrate first appeared on Bio-Radiations.]]> From megaTALs to CRISPR: The Many Ways to Edit a Gene https://www.bioradiations.com/from-megatals-to-crispr-the-many-ways-to-edit-a-gene/ Thu, 13 Oct 2016 17:37:15 +0000 http://www.bioradiations.com/?p=8800 CRISPR has revolutionized gene editing but is not the only way to edit a gene. We compare the pros and cons of four main gene editing techniques – meganucleases, zinc finger nucleases, TALENs, and CRISPR-Cas9 – discuss the advance of these methods to the clinic for therapeutic applications, and introduce newer technologies that eliminate some of the pitfalls of current techniques.

The post From megaTALs to CRISPR: The Many Ways to Edit a Gene first appeared on Bio-Radiations.]]> Coupling Automated Chromatography with Faster Confirmation of Purity and Yield https://www.bioradiations.com/coupling-automated-chromatography-with-faster-confirmation-of-purity-and-yield/ Thu, 13 Oct 2016 17:36:47 +0000 http://www.bioradiations.com/?p=8833 We’ve combined the chromatography process with purity and yield assessment using stain-free gels to shorten and optimize your protein purification workflow. See how it’s done.

The post Coupling Automated Chromatography with Faster Confirmation of Purity and Yield first appeared on Bio-Radiations.]]> Immunoprecipitation (IP) – Complex or Not? https://www.bioradiations.com/immunoprecipitation-ip-complex-or-not/ Thu, 13 Oct 2016 17:33:38 +0000 http://www.bioradiations.com/?p=8979 Provides an overview of the IP process and hands-on-advice on how to design and control IP experiments.

The post Immunoprecipitation (IP) – Complex or Not? first appeared on Bio-Radiations.]]> Cleaning the Nozzle Tip on the S3e™ Cell Sorter https://www.bioradiations.com/cleaning-the-nozzle-tip-on-the-s3e-cell-sorter/ Thu, 15 Sep 2016 23:20:54 +0000 http://www.bioradiations.com/?p=8797 In this short video, we show how to clean the nozzle tip on the S3e Cell Sorter.

The post Cleaning the Nozzle Tip on the S3e™ Cell Sorter first appeared on Bio-Radiations.]]> Monocytes — a Mini Review https://www.bioradiations.com/monocytes-a-mini-review/ Thu, 15 Sep 2016 23:18:26 +0000 http://www.bioradiations.com/?p=8795 Monocytes play key roles in mediating innate and adaptive immune responses. Here is an overview of the types, functions, process of recruitment, and the role of monocytes in disease.

The post Monocytes — a Mini Review first appeared on Bio-Radiations.]]> Six Tips for Efficient Transfer of Your Proteins https://www.bioradiations.com/six-tips-for-efficient-transfer-of-your-proteins/ Thu, 15 Sep 2016 23:14:49 +0000 http://www.bioradiations.com/?p=8783 A good and complete transfer of proteins from gels to membranes is crucial for the success of western blotting. Here are six useful tips for successful transfer of proteins.

The post Six Tips for Efficient Transfer of Your Proteins first appeared on Bio-Radiations.]]> Performing a Fluidics Hot Swap on the S3e™ Cell Sorter https://www.bioradiations.com/performing-a-fluidics-hot-swap-on-the-s3e-cell-sorter/ Thu, 15 Sep 2016 23:13:47 +0000 http://www.bioradiations.com/?p=8790 In this video, we show how a fluidics hot swap can be performed without shutting down the S3e Cell Sorter.

The post Performing a Fluidics Hot Swap on the S3e™ Cell Sorter first appeared on Bio-Radiations.]]> Cleaning the Streams Camera Window on the S3e™ Cell Sorter https://www.bioradiations.com/cleaning-the-streams-camera-window-on-the-s3e-cell-sorter/ Thu, 15 Sep 2016 23:10:43 +0000 http://www.bioradiations.com/?p=8787 In this video we show you how to clean the camera streams window as part of routine maintenance for your S3e Cell Sorter.

The post Cleaning the Streams Camera Window on the S3e™ Cell Sorter first appeared on Bio-Radiations.]]> Productivity through Workflow Personalization: What Pharma Is Learning from Other Industries https://www.bioradiations.com/productivity-through-workflow-personalization-what-pharma-is-learning-from-other-industries/ Fri, 12 Aug 2016 04:50:10 +0000 http://www.bioradiations.com/?p=8773 Striving for efficiency in workflow is not a new concept. Automation and use of smart technologies in industries such as automotive and shipping, and services like USPS and UPS, have drastically changed the way they function today not just with innovation but with personalization. Pharma is beginning to understand the need for adopting personalized workflow as well. Now, what can Pharma learn from these industries and how can it apply it to the industry?

The post Productivity through Workflow Personalization: What Pharma Is Learning from Other Industries first appeared on Bio-Radiations.]]> Attaching a Dynaloop to the NGC™ Chromatography System https://www.bioradiations.com/attaching-a-dynaloop-to-the-ngc-chromatography-system/ Fri, 12 Aug 2016 04:47:07 +0000 http://www.bioradiations.com/?p=8770 This video describes the three main steps in attaching a DynaLoop Sample Loop to the NGC™ Chromatography System to enable the injection of larger sample volumes: connecting the DynaLoop Sample Loop to the NGC Chromatography System, priming the DynaLoop Sample Loop, and creating a method in ChromLab™ Software.

The post Attaching a Dynaloop to the NGC™ Chromatography System first appeared on Bio-Radiations.]]> Calibrating the pH Valve on the NGC™ Chromatography System https://www.bioradiations.com/calibrating-the-ph-valve-on-the-ngc-chromatography-system/ Fri, 12 Aug 2016 04:44:47 +0000 http://www.bioradiations.com/?p=8767 The NGC pH valve provides real-time, in-line monitoring of buffer pH. In this video, you’ll learn how to connect the pH valve to the NGC System, add it to the flow path, and calibrate the valve using buffers at two different pH values. Features of the pH valve include accessible calibration ports and easy bypass of the probe when the NGC System is being cleaned.

The post Calibrating the pH Valve on the NGC™ Chromatography System first appeared on Bio-Radiations.]]> Tips and Strategies for Successful Purification of Your Proteins https://www.bioradiations.com/tips-and-strategies-for-successful-purification-of-your-proteins/ Fri, 12 Aug 2016 04:36:16 +0000 http://www.bioradiations.com/?p=8757 A biomolecule protein purification workflow requires more than one resin. The trick is to find the combination of resins that provides the highest purity in the least number of steps and at the lowest cost. Here are some guides to select the optimal resins for the purification of monoclonal antibodies, native and recombinant proteins, Ig G and mammalian and adenovirus, and for removing aggregates and endotoxins.

The post Tips and Strategies for Successful Purification of Your Proteins first appeared on Bio-Radiations.]]> Apoptosis https://www.bioradiations.com/apoptosis/ Fri, 12 Aug 2016 04:28:11 +0000 http://www.bioradiations.com/?p=8754 Differentiating between healthy and apoptic cells.

The post Apoptosis first appeared on Bio-Radiations.]]> Mastering IHC Staining Experiments https://www.bioradiations.com/mastering-ihc-staining-experiments/ Fri, 12 Aug 2016 04:18:05 +0000 http://www.bioradiations.com/?p=8749 Best practice for sample preparation, staining, and analysis.

The post Mastering IHC Staining Experiments first appeared on Bio-Radiations.]]> Optimize Your Flow Cytometry https://www.bioradiations.com/optimize-your-flow-cytometry/ Fri, 12 Aug 2016 04:16:09 +0000 http://www.bioradiations.com/?p=8746 Best practice for sample preparation, staining, and analysis.

The post Optimize Your Flow Cytometry first appeared on Bio-Radiations.]]> Cats Make Logical Inferences https://www.bioradiations.com/cats-make-logical-inferences/ Fri, 12 Aug 2016 04:12:27 +0000 http://www.bioradiations.com/?p=8741 People who live with cats attribute a lot of calculating behaviors to them. If this includes thinking your cat has a fundamental grasp of cause and effect, then you’re backed up by recent research by Saho Takagi and colleagues at Kyoto University.

The post Cats Make Logical Inferences first appeared on Bio-Radiations.]]> Assessing Cell Health: Apoptosis https://www.bioradiations.com/assessing-cell-health-apoptosis/ Fri, 15 Jul 2016 21:30:37 +0000 http://www.bioradiations.com/?p=8712 In this second part of the series on assessing the health of your cells, we review the different types and stages of apoptosis, and the various methods of assessing apoptosis of cells.

The post Assessing Cell Health: Apoptosis first appeared on Bio-Radiations.]]> Macrophage Polarization — An Overview https://www.bioradiations.com/macrophage-polarization-an-overview/ Fri, 15 Jul 2016 21:28:12 +0000 http://www.bioradiations.com/?p=8727 Macrophages are important players in the phagocytosis process in the immune system. In this detailed review, we provide an overview on the different types of macrophages, the signaling molecules involved in the polarization of M1 and M2 macrophage subsets, and more.

The post Macrophage Polarization — An Overview first appeared on Bio-Radiations.]]> Synthesis and Purification of Recombinant Proteins — A Success Story https://www.bioradiations.com/synthesis-and-purification-of-recombinant-proteins-a-success-story/ Fri, 15 Jul 2016 21:24:43 +0000 http://www.bioradiations.com/?p=8730 Producing highly purified proteins in large quantities is a challenge and requires modification of methods and protocols to attain high efficiency. Dr Murat Kasap from Kocaeli University, Turkey, has overcome these difficulties by taking advantage of the modularity of the NGC Chromatography System and successfully synthesized and purified cardiac troponin-I protein.

The post Synthesis and Purification of Recombinant Proteins — A Success Story first appeared on Bio-Radiations.]]> Removing Air Bubbles from the NGC™ Chromatography System https://www.bioradiations.com/removing-air-bubbles-from-the-ngc-chromatography-system/ Fri, 15 Jul 2016 21:21:39 +0000 http://www.bioradiations.com/?p=8723 This video explains how to remove bubbles and/or reduce bubble formation in your NGC System. Tips to reduce bubbles include warming up your multiwave detector for 30 to 60 min before use and flowing buffer at 0.1–0.2 ml/min in the manual mode until a stable/flat baseline is achieved before starting a method. The system may also be flushed with 100% methanol or ethanol at purge speed, with columns out of line, to remove bubbles.

The post Removing Air Bubbles from the NGC™ Chromatography System first appeared on Bio-Radiations.]]> Using the Air Sensor on the NGC™ Chromatography System https://www.bioradiations.com/using-the-air-sensor-on-the-ngc-chromatography-system/ Fri, 15 Jul 2016 21:09:24 +0000 http://www.bioradiations.com/?p=8705 This video describes the functions of the Air Sensor Module and how to connect it to the NGC Chromatography System. The NGC Air Sensor senses the end of sample or buffer and prevents the introduction of air into the system, which can damage columns and/or incur the loss of precious samples. Both small and large air sensors are available to fit the tubing being used. Steps for connecting the Air Sensor Module and associated air sensors are described including how to add the air sensors into the system flow path using ChromLab™ Software and the differences in using the air sensors in manual vs. method modes.

The post Using the Air Sensor on the NGC™ Chromatography System first appeared on Bio-Radiations.]]> Teach Your Hatchlings Well https://www.bioradiations.com/teach-your-hatchlings-well/ Fri, 15 Jul 2016 21:05:41 +0000 http://www.bioradiations.com/?p=8701 Baby songbirds learn their songs more quickly if their adult-bird teachers use baby talk, says research from Jon Sakata and colleagues at McGill University. Baby zebra finches learned songs significantly faster when taught by adult birds, as opposed to recordings of birds, and the team found that the reason was that real birds altered their songs in ways similar to human adults talking to small children, slowing and repeating phrases.

The post Teach Your Hatchlings Well first appeared on Bio-Radiations.]]> A Superior Method for Parallel Analysis of RNA and Protein Expression from Cell Culture Lysates https://www.bioradiations.com/a-superior-method-for-parallel-analysis-of-rna-and-protein-expression-from-cell-culture-lysates/ Thu, 16 Jun 2016 23:33:14 +0000 http://www.bioradiations.com/?p=8684 It is imperative to analyze both the transcriptional and translational profiles for a complete understanding of the functioning of genes. Here we describe a simple protocol that enables parallel analysis of both RNA and protein from single cell culture lysates. See how this workflow could save time and money and yield more reliable data than the traditional protocols used.

The post A Superior Method for Parallel Analysis of RNA and Protein Expression from Cell Culture Lysates first appeared on Bio-Radiations.]]> Assessing Cell Health: Viability and Proliferation https://www.bioradiations.com/assessing-cell-health-viability-and-proliferation/ Thu, 16 Jun 2016 23:16:59 +0000 http://www.bioradiations.com/?p=8669 In this multi-part series on assessing the health of your cells, we review different factors to be considered in your assessments such as cell viability, proliferation, apoptosis, and autophagy. The first part covers the methods for assessing viability and proliferation and their advantages, and discusses some common considerations.

The post Assessing Cell Health: Viability and Proliferation first appeared on Bio-Radiations.]]> Purifying Difficult-to-Purify Proteins https://www.bioradiations.com/purifying-difficult-to-purify-proteins/ Thu, 16 Jun 2016 23:12:00 +0000 http://www.bioradiations.com/?p=8665 Protein purification becomes a challenging step for applications requiring high protein concentrations (such as structural biology studies). This is also true for researchers purifying integral membranes proteins and untagged proteins from native sources. Here we provide some useful tips for selecting the proper resins, optimizing conditions and developing effective strategies to tackle the challenges associated with such difficult-to-purify proteins.

The post Purifying Difficult-to-Purify Proteins first appeared on Bio-Radiations.]]> Tips for Selecting the Best Gel for Your Application https://www.bioradiations.com/tips-for-selecting-the-best-gel-for-your-application/ Thu, 16 Jun 2016 23:05:15 +0000 http://www.bioradiations.com/?p=8655 Picking the right gel for your application is not easy. It depends on so many factors, such as the purpose of your application, the size of your protein, what kind of buffer you need to have in your sample etc. Here we provide a guide for you to choose the best Bio-Rad gel based on all these factors.

The post Tips for Selecting the Best Gel for Your Application first appeared on Bio-Radiations.]]> Research Review: Phasing Genomes using Droplet Digital™ PCR https://www.bioradiations.com/research-review-phasing-genomes-using-droplet-digital-pcr/ Thu, 16 Jun 2016 22:05:29 +0000 http://www.bioradiations.com/?p=8659 Find out how a new Droplet Digital PCR-based technique called Drop Phase lets you quickly and easily phase genomes to better understand diseases like cystic fibrosis.

The post Research Review: Phasing Genomes using
Droplet Digital™ PCR first appeared on Bio-Radiations.]]> Birds of a Feather Flock According to Weather? https://www.bioradiations.com/birds-of-a-feather-flock-according-to-weather/ Wed, 15 Jun 2016 21:53:25 +0000 http://www.bioradiations.com/?p=8651 Polymorphic animals are those that have distinctly different outward traits, such as a color, within a species. Black sparrowhawks (Accipiter melanoleucus)), despite their common name, exist in a version with a white front as well as an all-dark version. But why would evolution simultaneously favor such contrasting color variations within the species?

The post Birds of a Feather Flock According to Weather? first appeared on Bio-Radiations.]]> Bioradiations May 2016 Edition https://www.bioradiations.com/bioradiations-may-2016-edition/ Thu, 12 May 2016 18:50:53 +0000 http://www.bioradiations.com/?p=8636 See what new articles and videos are in the May edition of Bioradiations.

The post Bioradiations May 2016 Edition first appeared on Bio-Radiations.]]> Rapid Advances in Biotechnology Bring Questions about Patentability https://www.bioradiations.com/rapid-advances-in-biotechnology-bring-questions-about-patentability/ Wed, 11 May 2016 20:47:05 +0000 http://www.bioradiations.com/?p=8628 Striking a balance between encouraging innovation and allowing access to knowledge is not simple. Allowing patents for modifications of naturally occurring organisms and cells pose a particular challenge to the scientific community. Adding to the complexity is the variations in regulations between countries. This article discusses these issues with specific examples in biotechnology and biomedical fields.

The post Rapid Advances in Biotechnology Bring Questions about Patentability first appeared on Bio-Radiations.]]> The Importance of Quality PCR Plastics https://www.bioradiations.com/the-importance-of-quality-pcr-plastics/ Wed, 11 May 2016 20:44:19 +0000 http://www.bioradiations.com/?p=8621 Do we ever think seriously about PCR plates, seals, and tubes as contributing to the quality of our gene expression results? Chances are that these PCR plastics are viewed just as labware by most people. In this article we bring out how simple factors, such as PCR plate rigidity, color of the wells, and adhesiveness of plate seals, can affect your gene expression results and show why it is important to use quality PCR plastics.

The post The Importance of Quality PCR Plastics first appeared on Bio-Radiations.]]> How Do You Automate a Multicolumn Protein Purification? https://www.bioradiations.com/how-do-you-automate-a-multicolumn-protein-purification/ Wed, 11 May 2016 20:37:44 +0000 http://www.bioradiations.com/?p=8618 Multicolumn purifications are tedious and the manual operations during this process introduce variabilities, affecting the success of purification and reproducibility of results. This whiteboard animation explains how an automated multi-dimensional chromatography method can make this an easier process with very high success rates.

The post How Do You Automate a Multicolumn Protein Purification? first appeared on Bio-Radiations.]]> #5 General Tips to Make your Multiplex Experiments Easier https://www.bioradiations.com/5-general-tips-to-make-your-multiplex-experiments-easier/ Wed, 11 May 2016 20:32:00 +0000 http://www.bioradiations.com/?p=8615 Here are some general tips to make your multiplex immunoassay experiments successful.

The post #5 General Tips to Make your Multiplex Experiments Easier first appeared on Bio-Radiations.]]> Are You Vegetarian? Could Be Something in Your Genes https://www.bioradiations.com/are-you-vegetarian-could-be-something-in-your-genes/ Wed, 11 May 2016 20:25:57 +0000 http://www.bioradiations.com/?p=8613 A recent study published in Molecular Biology and Evolution describes a phenomenon where populations that have typically consumed vegetarian diets over hundreds of generations have evolved a “vegetarian gene”.

The post Are You Vegetarian? Could Be Something in Your Genes first appeared on Bio-Radiations.]]> Do Lizards Dream of Scaly Sheep? https://www.bioradiations.com/do-lizards-dream-of-scaly-sheep/ Wed, 11 May 2016 20:20:04 +0000 http://www.bioradiations.com/?p=8633 If you rouse a sleeping dragon, you might be interrupting its dreams. Or at least that’s the suggestion from recent studies at the Max Planck Institute for Brain Research. A team led by Gilles Laurent set out to learn how the Australian dragon (Pogona vitticeps) responds to visual information, but continuous monitoring of brain activity with electrodes revealed other intriguing data. When sleeping, the reptiles’ brains emitted alternating patterns that resembled the switching between slow-wave deep sleep and rapid eye movement (REM) observed in birds and mammals, including humans. The alternation in reptile brains was quicker and more regular than in birds and mammals, but otherwise comparable. The team made additional observations that bolstered their suspicion that the lizards were experiencing REM, and they discovered additional similarities to mammal and bird sleep, such as coordination between regions of the brain.

The post Do Lizards Dream of Scaly Sheep? first appeared on Bio-Radiations.]]> Overcoming Purification Hurdles with Mixed-Mode Chromatography https://www.bioradiations.com/overcoming-purification-hurdles-with-mixed-mode-chromatogprahy/ Wed, 04 May 2016 16:49:31 +0000 http://www.bioradiations.com/?p=8966 Dr. Mark Snyder, a leading expert in the field of protein purification, presents the purification process development for two very different targets, adenovirus and bacterial surface proteins. In both cases, ...

The post Overcoming Purification Hurdles with Mixed-Mode Chromatography first appeared on Bio-Radiations.]]> #4 Steps to a Successful Cell and Tissue Homogenates Prep for Multiplex Assays https://www.bioradiations.com/4-steps-to-a-successful-cell-and-tissue-homogenates-prep-for-multiplex-assays/ Wed, 13 Apr 2016 01:10:34 +0000 http://www.bioradiations.com/?p=8584 Explore this protocol for cell and tissue homogenate prep for multiplex assays.

The post #4 Steps to a Successful Cell and Tissue Homogenates Prep for Multiplex Assays first appeared on Bio-Radiations.]]> Expanding Flow Core Capabilities with the S3e™ Cell Sorter https://www.bioradiations.com/expanding-flow-core-capabilities-with-the-s3e-cell-sorter/ Wed, 13 Apr 2016 01:00:38 +0000 http://www.bioradiations.com/?p=8574 Andy Riddell, Flow Cytometry Core Facility Manager at the Wellcome Trust- MRC Stem Cell Institutes, is expanding his core facility by allowing his researchers to conduct sorting on their own. In doing so, he is now free to explore and answer the challenging flow cytometry questions that require more of his expert time. Find out how the S3e Cell Sorter has allowed Andy that freedom and how easy sorting can be for researchers.

The post Expanding Flow Core Capabilities with the S3e™ Cell Sorter first appeared on Bio-Radiations.]]> Ravens Get Inside Each Other’s Heads https://www.bioradiations.com/ravens-get-inside-each-others-heads/ Wed, 13 Apr 2016 00:57:12 +0000 http://www.bioradiations.com/?p=8569 New research suggests that ravens understand that others have minds. Theory of mind, the ability to attribute perceptions and mental states to others, either like or different from one’s own, has previously been assumed to be the domain of humans only.

The post Ravens Get Inside Each Other’s Heads first appeared on Bio-Radiations.]]> Bioradiations April 2016 Edition https://www.bioradiations.com/bioradiations-april-2016-edition/ Wed, 13 Apr 2016 00:41:03 +0000 http://www.bioradiations.com/?p=8566 See what new articles and videos are in the April edition of Bioradiations.

The post Bioradiations April 2016 Edition first appeared on Bio-Radiations.]]> Expanding Flow Core Capabilities by Shifting Routine Sorts to Self-Sort on the S3e™ Cell Sorter https://www.bioradiations.com/expanding-flow-core-capabilities-by-shifting-routine-sorts-to-self-sort-on-the-s3e-cell-sorter/ Tue, 12 Apr 2016 20:50:48 +0000 http://www.bioradiations.com/?p=8591 To run a core flow cytometry lab is no easy task. There is always a balance between running simple sorts and developing new assays for answering complicated questions that demand pushing the limits of existing assays and the limitations of the sorters. See how Andy Riddell, a flow core manager, pushes the limits of his existing assays and how Bio-Rad’s S3e™ cell sorter is allowing him to accomplish that.

The post Expanding Flow Core Capabilities by Shifting Routine Sorts to Self-Sort on the S3e™ Cell Sorter first appeared on Bio-Radiations.]]> Western Blot Detection without Interfering IgGs: A Mere Fantasy or a Real Possibility? https://www.bioradiations.com/western-blot-detection-without-interfering-iggs-a-mere-fantasy-or-a-real-possibility/ Tue, 12 Apr 2016 20:00:23 +0000 http://www.bioradiations.com/?p=8577 Interference from IgG heavy (~ 50 kD) or light (~25 kD) chains is very common when trying to visualize bands of immunoprecipitated proteins. If your band of interest has a molecular weight close to these, it becomes really hard to distinguish between the IgG and the band of interest. How could this be avoided? Bio-Rad’s new TidyBlot™ Western Blot Detection Reagent accomplishes this by binding exclusively to the native nondenatured antibodies rather than any IgG in the sample. Explore the performance characteristics of this new reagent.

The post Western Blot Detection without Interfering IgGs: A Mere Fantasy or a Real Possibility? first appeared on Bio-Radiations.]]> Characterizing Functional Domains of Potential Therapeutics with Better Protein Purification https://www.bioradiations.com/characterizing-functional-domains-of-potential-therapeutics-with-better-protein-purification/ Wed, 23 Mar 2016 17:03:24 +0000 http://www.bioradiations.com/?p=8974 Dr. John Bruning (University of Adelaide) and Dr. Jiansheng Wu (Genentech), two leading experts in the field of protein purification, share exciting examples of their research and recent chromatography tools they have used to demonstrate robust and improved efficiencies to their discovery and characterization protein purification workflows.

The post Characterizing Functional Domains of Potential Therapeutics with Better Protein Purification first appeared on Bio-Radiations.]]> Bioradiations March 2016 Edition https://www.bioradiations.com/bioradiations-march-2016-edition/ Thu, 10 Mar 2016 21:13:01 +0000 http://www.bioradiations.com/?p=8554 See what new articles and videos are in the March edition of Bioradiations.

The post Bioradiations March 2016 Edition first appeared on Bio-Radiations.]]> Using PrimePCR™ Gene Expression Assays to Understand Breast Cancer Metastasis https://www.bioradiations.com/using-primepcr-gene-expression-assays-to-understand-breast-cancer-metastasis/ Tue, 08 Mar 2016 18:47:06 +0000 http://www.bioradiations.com/?p=8546 Tumor metastasis is a complex process. It requires the ability of the cancer cells to invade their surroundings and travel to distant sites and survive. Dr. Traci Lyons studies the mechanism of COX-2 functioning in the metastasis of breast cancer cells. Find out how Dr. Lyons used Bio-Rad’s PrimePCR™ Assays to confirm COX-2 knockdown at the mRNA level.

The post Using PrimePCR™ Gene Expression Assays to Understand Breast Cancer Metastasis first appeared on Bio-Radiations.]]> 8 Tips for Understanding Multiplex Immunoassay Development and Validation https://www.bioradiations.com/8-tips-for-understanding-multiplex-immunoassay-development-and-validation/ Tue, 08 Mar 2016 18:40:39 +0000 http://www.bioradiations.com/?p=8542 Multiplex immunoassay development requires a lot of validation and standardization steps. Several factors can influence the performance of these assays. Some factors that need to be considered are mentioned here.

The post 8 Tips for Understanding Multiplex Immunoassay Development and Validation first appeared on Bio-Radiations.]]> How Does an Antibody Qualify To Become a PrecisionAb™ Antibody? https://www.bioradiations.com/how-does-an-antibody-qualify-to-become-a-precisionab-antibody/ Tue, 08 Mar 2016 18:34:37 +0000 http://www.bioradiations.com/?p=8533 Not all antibodies are created equal. Some have to work hard to qualify to become Bio-Rad’s PrecisionAb™ Antibody. Here is a diagrammatic representation of how the qualification criteria are determined.

The post How Does an Antibody Qualify To Become a PrecisionAb™ Antibody? first appeared on Bio-Radiations.]]> Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison of CHT™ Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes https://www.bioradiations.com/mixed-mode-chromatography-for-mab-s-aggregate-removal-comparison-of-cht-ceramic-hydroxyapatite-capto-adhere-and-capto-adhere-impres/ Tue, 08 Mar 2016 18:26:30 +0000 http://www.bioradiations.com/?p=8507 The separation of monomers from product-related impurities is quite challenging in process separations. It requires a thorough screening and an efficient resin to get efficiency in the purification process. Review the performance data of Bio-Rad’s mixed mode CHT™ Ceramic Hydroxapatite in achieving process separation efficiency and how it compares with various Capto media.

The post Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison of CHT™ Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes first appeared on Bio-Radiations.]]> #3 Steps to a Successful Cell Culture Supernatant Prep for Multiplex Immunoassays https://www.bioradiations.com/3-steps-to-a-successful-cell-culture-supernatant-prep-for-multiplex-immunoassays/ Tue, 08 Mar 2016 08:00:55 +0000 http://www.bioradiations.com/?p=8496 Here is a quick protocol for preparing a cell culture supernatant sample for multiplex biomarker analysis.

The post #3 Steps to a Successful Cell Culture Supernatant Prep for Multiplex Immunoassays first appeared on Bio-Radiations.]]> Using PrimePCR™ qPCR Assays to Understand Breast Cancer Metastasis https://www.bioradiations.com/using-primepcr-qpcr-assays-to-understand-breast-cancer-metastasis/ Tue, 08 Mar 2016 08:00:43 +0000 http://www.bioradiations.com/?p=8501 Traci Lyons describes how PrimePCR Assays ended her laboratory’s struggle to find a qPCR assay that could reliably detect transcription of the cyclooxygenase-2 (Cox-2) gene, which they had identified as potentially prometastatic. Using the PrimePCR Assay for Cox-2, researchers in her lab were finally able to verify cyclooxygenase-2 knockdown, which is a necessary step toward confirming its role in breast cancer metastasis.

The post Using PrimePCR™ qPCR Assays to Understand Breast Cancer Metastasis first appeared on Bio-Radiations.]]> A New Source for Mutations Found in Tumors https://www.bioradiations.com/a-new-source-for-mutations-found-in-tumors/ Mon, 07 Mar 2016 19:43:36 +0000 http://www.bioradiations.com/?p=8493 A study recently published in the Proceedings of the National Academy of Sciences furthers our understanding of tumors and the source of the mutations that are found within their DNA. This study, carried out by researchers at Wayne State University and Indiana University, highlights a new mutation signature found in cancer cells that is caused by members of the APOBEC3 family of proteins.

The post A New Source for Mutations Found in Tumors first appeared on Bio-Radiations.]]> Using Sound Waves as Tweezers to Move Single Cells https://www.bioradiations.com/study-explains-how-one-enzyme-can-synthesize-all-four-dna-bases-3/ Mon, 07 Mar 2016 19:39:59 +0000 http://www.bioradiations.com/?p=8491 A single enzyme, ribonucleotide reductase (RNR), is responsible for making all four deoxyribonucleotides (dNTPs) and ensuring that they are present at the ratios required to synthesize new DNA. This is possible because RNRs change their substrate specificity in response to dNTP abundance. For example, when dGTP is abundant it binds RNR, increase its affinity for ADP, and thus shifts synthesis to dATP. Scientists in the Drennan lab at the Massachusetts Institute of Technology have long been fascinated by this unusual enzyme and now describe the allosteric changes that underlie RNR’s ability to shift specificity and maintain the proper dNTP pool in our cells.

The post Using Sound Waves as Tweezers to Move Single Cells first appeared on Bio-Radiations.]]> Bat Signal Precision https://www.bioradiations.com/bat-signal-precision/ Mon, 07 Mar 2016 19:37:58 +0000 http://www.bioradiations.com/?p=8488 Imagine you’re singing the Batman theme song in a large chorus and you need to identify your own voice in the playback. Sounds difficult, but it is similar to what individual bats in colonies must do to navigate and find tiny morsels of food such as insects via echolocation. New research from a team at Tel Aviv University led by Yossi Yovel indicates they do this by modifying the volume and duration of the calls and then following the echo that exactly matches. If you did this in your chorus, you’d pick out your own voice by knowing whether you were singing “batman,” “batmaan,” or “batmaaan.” The finding dispels the previous assumption that the bats were modifying the pitch of their voices.

The post Bat Signal Precision first appeared on Bio-Radiations.]]> Bioradiations February 2016 Edition https://www.bioradiations.com/bioradiations-february-2016-edition/ Thu, 11 Feb 2016 22:07:11 +0000 http://www.bioradiations.com/?p=8412 See what new articles and videos are in the February edition of Bioradiations.

The post Bioradiations February 2016 Edition first appeared on Bio-Radiations.]]> New Product Feature: gDNA-Free cDNA in Two Simple Steps https://www.bioradiations.com/new-product-feature-gdna-free-cdna-in-two-simple-steps/ Tue, 09 Feb 2016 22:08:23 +0000 http://www.bioradiations.com/?p=8460 Removing genomic DNA contamination from cDNA preparations is a challenge. All currently available methods are time consuming, could lead to sample loss, and are not efficient. Bio-Rad’s iScript™gDNA Clear cDNA Synthesis Kit tackles these issues and makes this step effortless. This article presents the performance data of the kit and describes how simple and efficient it is to use the kit for gDNA removal.

The post New Product Feature: gDNA-Free cDNA in Two Simple Steps first appeared on Bio-Radiations.]]> PrecisionAb™ Antibodies: Defining New Standards for Western Blotting https://www.bioradiations.com/precisionab-antibodies-defining-new-standards-for-western-blotting/ Tue, 09 Feb 2016 22:00:24 +0000 http://www.bioradiations.com/?p=8457 Choosing the right primary antibody is an arduous task. A lot of standardization needs to happen before we can determine that the antibody is specific and sensitive enough for the purpose of our experiment. The new PrecisonAb™ Antibodies from Bio-Rad go through vigorous testing and validation before they are deemed suitable, thus eliminating the process of trial and error for researchers. The video describes how the PrecisionAb antibodies are developed.

The post PrecisionAb™ Antibodies: Defining New Standards for Western Blotting first appeared on Bio-Radiations.]]> How Does the Mixed Mode Interaction of a Media Help Achieve High Selectivity and Resolution? https://www.bioradiations.com/how-does-the-mixed-mode-interaction-of-a-media-help-achieve-high-selectivity-and-resolution/ Tue, 09 Feb 2016 21:53:01 +0000 http://www.bioradiations.com/?p=8452 The best chromatography media should yield high protein purity and product recovery as well as be versatile enough to purify difficult samples. Bio-Rad’s CHT™ Ceramic Hydroxyapatite achieves this by virtue of being able to interact with the proteins through multiple modes, making the process extremely efficient. The whiteboard animation describes the mode of action of CHT.

The post How Does the Mixed Mode Interaction of a Media Help Achieve High Selectivity and Resolution? first appeared on Bio-Radiations.]]> Map of Human Lineage Biomarkers for Different Immune Cell Types https://www.bioradiations.com/map-of-human-lineage-biomarkers-for-different-immune-cell-types/ Tue, 09 Feb 2016 21:50:23 +0000 http://www.bioradiations.com/?p=8443 How can we easily identify biomarkers for different immune cell types? Refer to this color-coded poster for quick and simple identification of human lineage biomarkers.

The post Map of Human Lineage Biomarkers for Different Immune Cell Types first appeared on Bio-Radiations.]]> Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies https://www.bioradiations.com/western-blotting-without-trial-and-error-use-precisionab-antibodies/ Tue, 09 Feb 2016 21:47:40 +0000 http://www.bioradiations.com/?p=8439 Watch this video to see how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

The post Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies first appeared on Bio-Radiations.]]> #2 Steps for a Successful Serum or Plasma Preparations for Multiplex Biomarker Assays https://www.bioradiations.com/2-steps-for-a-successful-serum-or-plasma-preparations-for-multiplex-biomarker-assays/ Tue, 09 Feb 2016 20:13:40 +0000 http://www.bioradiations.com/?p=8421 Here is a quick protocol for preparing a serum or plasma sample for multiplex biomarker analysis.

The post #2 Steps for a Successful Serum or Plasma Preparations for Multiplex Biomarker Assays first appeared on Bio-Radiations.]]> Study Explains How One Enzyme Can Synthesize All Four DNA Bases https://www.bioradiations.com/study-explains-how-one-enzyme-can-synthesize-all-four-dna-bases/ Tue, 09 Feb 2016 19:41:15 +0000 http://www.bioradiations.com/?p=8418 A single enzyme, ribonucleotide reductase (RNR), is responsible for making all four deoxyribonucleotides (dNTPs) and ensuring that they are present at the ratios required to synthesize new DNA. This is possible because RNRs change their substrate specificity in response to dNTP abundance. For example, when dGTP is abundant it binds RNR, increase its affinity for ADP, and thus shifts synthesis to dATP. Scientists in the Drennan lab at the Massachusetts Institute of Technology have long been fascinated by this unusual enzyme and now describe the allosteric changes that underlie RNR’s ability to shift specificity and maintain the proper dNTP pool in our cells.

The post Study Explains How One Enzyme Can Synthesize All Four DNA Bases first appeared on Bio-Radiations.]]> The Beginning of the End for Ebola? https://www.bioradiations.com/the-beginning-of-the-end-for-ebola/ Tue, 09 Feb 2016 19:22:48 +0000 http://www.bioradiations.com/?p=8416 Researchers at Albert Einstein College of Medicine and the US Army Medical Research Institute of Infectious Diseases have discovered a potential immunotherapeutic treatment for two of the deadliest Ebola virus strains.

The post The Beginning of the End for Ebola? first appeared on Bio-Radiations.]]> Design for Learning: Developing Curricula for Instructional Lab Kits https://www.bioradiations.com/design-for-learning-developing-curricula-for-instructional-lab-kits/ Wed, 13 Jan 2016 01:55:45 +0000 http://www.bioradiations.com/?p=8389 How are educational curricula developed? Who sets the standards? How do companies that develop science education kits keep up with this and design their kits around the curricula? Find out in this article.

The post Design for Learning: Developing Curricula for Instructional Lab Kits first appeared on Bio-Radiations.]]> List of Mouse Lineage Biomarkers for Different Immune Cell Types https://www.bioradiations.com/list-of-mouse-lineage-biomarkers-for-different-immune-cell-types/ Wed, 13 Jan 2016 01:54:06 +0000 http://www.bioradiations.com/?p=8383 How can we easily identify biomarkers for different immune cell types? This color-coded poster makes identification of mouse lineage biomarkers easy.

The post List of Mouse Lineage Biomarkers for Different Immune Cell Types first appeared on Bio-Radiations.]]> Chromatography: Everything You Need to Know about Your Peaks https://www.bioradiations.com/chromatography-everything-you-need-to-know-about-your-peaks/ Wed, 13 Jan 2016 01:53:29 +0000 http://www.bioradiations.com/?p=8378 What can chromatography peaks tell us? From the quality of column packing to the purity of separated proteins, a lot can be gleaned from the peaks. Here are some definitions that can help decipher what chromatography peaks can tell us.

The post Chromatography: Everything You Need to Know about Your Peaks first appeared on Bio-Radiations.]]> How to Design Hydrolysis Probes for qPCR https://www.bioradiations.com/how-to-design-hydrolysis-probes-for-qpcr/ Wed, 13 Jan 2016 01:52:26 +0000 http://www.bioradiations.com/?p=8394 What's the optimal melting temperature for a hydrolysis probe? How long should your probe be? And why should you avoid 5' guanines like the plague? This episode answers these questions and gives you five simple tricks for designing the perfect hydrolysis probe.

The post How to Design Hydrolysis Probes for qPCR first appeared on Bio-Radiations.]]> #1 May the Plex Be with You — Serum or Plasma: What Sample Do I Choose for My Multiplex Biomarker Assays? https://www.bioradiations.com/1-may-the-plex-be-with-you-serum-or-plasma-what-sample-do-i-choose-for-my-multiplex-biomarker-assays/ Wed, 13 Jan 2016 01:51:22 +0000 http://www.bioradiations.com/?p=8365 Find out why your multiplex assay measurements can differ for serum and plasma samples.

The post #1 May the Plex Be with You — Serum or Plasma: What Sample Do I Choose for My Multiplex Biomarker Assays? first appeared on Bio-Radiations.]]> A Pain-Free Life: How Some People Are Unable to Feel Pain https://www.bioradiations.com/a-pain-free-life-how-some-people-are-unable-to-feel-pain/ Wed, 13 Jan 2016 01:50:06 +0000 http://www.bioradiations.com/?p=8368 Individuals born with a rare gene mutation that leads to a nonfunctional sodium channel Nav1.7 are unable to feel pain. Although previous attempts to develop an analgesic drug by blocking Nav1.7 have been largely unsuccessful, researchers at University College London discovered recently that Nav1.7-deficient humans and transgenic mice had elevated levels of opioid peptides. These opioids likely contribute to the painlessness and elucidate a potential analgesic approach that combines specific sodium channel blockade with a low dose of opioids.

The post A Pain-Free Life: How Some People Are Unable to Feel Pain first appeared on Bio-Radiations.]]> CRISPR-Cas9 Makes Studying Exotic Organisms Much Easier https://www.bioradiations.com/crispr-cas9-makes-studying-exotic-organisms-much-easier/ Wed, 13 Jan 2016 01:49:28 +0000 http://www.bioradiations.com/?p=8370 The CRISPR-Cas9 system has been making major waves in the field of gene editing for some time now. Now, researchers at the University of California, Berkeley have shown that not only is this gene-editing tool transforming the field of basic science research, but it can have a great impact on genetic studies in more exotic organisms like slime mold and octopi.

The post CRISPR-Cas9 Makes Studying Exotic Organisms Much Easier first appeared on Bio-Radiations.]]> Making CRISPR-Targeted Genome Editing Safer https://www.bioradiations.com/making-crispr-targeted-genome-editing-safer-2/ Wed, 13 Jan 2016 01:48:21 +0000 http://www.bioradiations.com/?p=8372 The CRISPR-Cas9 technology is a powerful system for genome editing. It works by making targeted modifications to DNA. Guided by short RNAs known as CRISPR RNA or crRNA, the Cas9 protein is targeted to a specific sequence that is complementary to the crRNA sequence. Cas9 is highly efficient at cleaving at its target site. However, off-target cleavage, that is, binding to and cutting sites that are not fully complementary to the guide RNA, is a major drawback of the system.

The post Making CRISPR-Targeted Genome Editing Safer first appeared on Bio-Radiations.]]> Bioradiations January Edition https://www.bioradiations.com/bioradiations-january-edition/ Wed, 13 Jan 2016 01:47:26 +0000 http://www.bioradiations.com/?p=8375 See what new articles and videos are in the January edition of Bioradiations.

The post Bioradiations January Edition first appeared on Bio-Radiations.]]> Top Stories of 2015 — Bioradiations Year in Review https://www.bioradiations.com/top-stories-of-2015-bioradiations-year-in-review/ Tue, 08 Dec 2015 19:15:11 +0000 http://www.bioradiations.com/?p=8126 In 2015, we published an extensive array of tips, protocols, videos, articles of general interest, customer testimonials, and technical and application notes on bioradiations.com. Here is a list of the most popular 2015 stories.

The post Top Stories of 2015 — Bioradiations Year in Review first appeared on Bio-Radiations.]]> So, How Can the Sso7d Fusion Polymerase Technology Help Your PCR? https://www.bioradiations.com/so-how-can-the-sso7d-fusion-polymerase-technology-help-your-pcr/ Tue, 08 Dec 2015 19:10:10 +0000 http://www.bioradiations.com/?p=8118 During a PCR reaction, it is imperative for the DNA polymerase to remain attached to the template while extending the new strand. This association is jeopardized under challenging PCR conditions and when amplifying longer DNA fragments. See how Sso7d, a small protein capable of binding with the polymerase, helps in stabilizing this association and increasing PCR efficiency.

The post So, How Can the Sso7d Fusion Polymerase Technology Help Your PCR? first appeared on Bio-Radiations.]]> Flow Cytometry: Basic Definitions https://www.bioradiations.com/flow-definitions/ Tue, 08 Dec 2015 19:07:09 +0000 http://www.bioradiations.com/?p=8114 It’s always good to get the basics straight. Here, we’ve defined some flow cytometry terms that might have eluded you.

The post Flow Cytometry: Basic Definitions first appeared on Bio-Radiations.]]> #7 How Do You Detect DNA Contamination in Your Protein During Purification? https://www.bioradiations.com/7-how-do-you-detect-dna-contamination-in-your-protein-during-purification/ Tue, 08 Dec 2015 19:03:58 +0000 http://www.bioradiations.com/?p=8112 Here are some ways you can detect DNA contamination during the protein purification process.

The post #7 How Do You Detect DNA Contamination in Your Protein During Purification? first appeared on Bio-Radiations.]]> Six Tips for Increasing the Reproducibility of qPCR Experiments https://www.bioradiations.com/six-tips-for-increasing-the-reproducibility-of-qpcr-experiments/ Tue, 08 Dec 2015 19:02:53 +0000 http://www.bioradiations.com/?p=8105 Follow these six simple tips and you'll increase the reproducibility of your qPCR experiments.

The post Six Tips for Increasing the Reproducibility of qPCR Experiments first appeared on Bio-Radiations.]]> High-Processivity Sso7d Fusion Polymerase Improves qPCR Results https://www.bioradiations.com/high-processivity-sso7d-fusion-polymerase-improves-qpcr-results/ Tue, 08 Dec 2015 19:01:56 +0000 http://www.bioradiations.com/?p=8108 Processivity is the rate at which nucleotides are added to extend PCR products. High processivity results in a greater number of nucleotides incorporated in each polymerase binding event.

The post High-Processivity Sso7d Fusion Polymerase Improves qPCR Results first appeared on Bio-Radiations.]]> Making CRISPR-Targeted Genome Editing Safer https://www.bioradiations.com/making-crispr-targeted-genome-editing-safer/ Tue, 08 Dec 2015 18:57:18 +0000 http://www.bioradiations.com/?p=8103 The CRISPR-Cas9 technology is a powerful system for genome editing. It works by making targeted modifications to DNA. Guided by short RNAs known as CRISPR RNA or crRNA, the Cas9 protein is targeted to a specific sequence that is complementary to the crRNA sequence. Cas9 is highly efficient at cleaving at its target site. However, off-target cleavage, that is, binding to and cutting sites that are not fully complementary to the guide RNA, is a major drawback of the system.

The post Making CRISPR-Targeted Genome Editing Safer first appeared on Bio-Radiations.]]> A Sticky Plant with a Clever Trick https://www.bioradiations.com/a-sticky-plant-with-a-clever-trick/ Tue, 08 Dec 2015 18:54:54 +0000 http://www.bioradiations.com/?p=8100 A garden book might note a columbine’s delicate leaves and gracefully nodding flowers, but at least one species, Aquilegia eximia, has been shown to have a trick or two up its sepal.

The post A Sticky Plant with a Clever Trick first appeared on Bio-Radiations.]]> What Day to Schedule Esophageal Cancer Surgery? https://www.bioradiations.com/what-day-to-schedule-esophageal-cancer-surgery/ Tue, 08 Dec 2015 18:53:42 +0000 http://www.bioradiations.com/?p=8098 If you’re undergoing surgery for esophageal cancer, you may want to schedule it for a Monday or Tuesday. Researchers at the Karolinska Institutet in Stockholm, Sweden found that patients who undergo esophageal cancer surgery early in the week have a greater chance of long-term survival than those who have surgery at the end of the week. These findings were recently published in the journal Annals of Surgery.

The post What Day to Schedule Esophageal Cancer Surgery? first appeared on Bio-Radiations.]]> Trained Pigeons Read Mammograms As Well As Humans https://www.bioradiations.com/trained-pigeons-read-mammograms-as-well-as-humans/ Tue, 08 Dec 2015 18:52:38 +0000 http://www.bioradiations.com/?p=8095 Contrary to conventional belief, having a “bird brain” may not be a bad thing — at least for identifying cancer on radiology images. A recent study from the University of Iowa and the University of California (UC) Davis, showed that trained pigeons performed as well as humans in identifying benign and malignant breast tissue on digitized slides and mammograms. The results help elucidate how physicians process visual cues on diagnostic images and highlight pigeons’ ability to interpret complex visual images.

The post Trained Pigeons Read Mammograms As Well As Humans first appeared on Bio-Radiations.]]> Bioradiations December Edition https://www.bioradiations.com/bioradiations-december-edition/ Tue, 08 Dec 2015 00:53:35 +0000 http://www.bioradiations.com/?p=8139 See what new articles and videos are in the November edition of Bioradiations.

The post Bioradiations December Edition first appeared on Bio-Radiations.]]> Bioradiations November Edition https://www.bioradiations.com/bioradiations-november-edition/ Thu, 12 Nov 2015 19:00:35 +0000 http://www.bioradiations.com/?p=8083 See what new articles and videos are in the November edition of Bioradiations.

The post Bioradiations November Edition first appeared on Bio-Radiations.]]> Bio-Rad’s PrimePCR™ Assays and Panels Eliminate Multiple Steps from Your qPCR Workflow https://www.bioradiations.com/bio-rads-primepcr-assays-and-panels-eliminate-multiple-steps-from-your-qpcr-workflow/ Tue, 10 Nov 2015 22:33:18 +0000 http://www.bioradiations.com/?p=7942 A qPCR workflow involves multiple steps, each of which adds to the time and cost of the entire experiment. The PrimePCR™ Assays and Panels from Bio-Rad eliminate a number of these steps with their predesigned primers and probes and help with the assay design. See what steps they eliminate and how that would help expedite your qPCR experiment.

The post Bio-Rad’s PrimePCR™ Assays and Panels Eliminate Multiple Steps from Your qPCR Workflow first appeared on Bio-Radiations.]]> In Pursuit of (Ig) Nobility: The Ig Nobel Prize https://www.bioradiations.com/in-pursuit-of-ig-nobility-the-ig-nobel-prize/ Tue, 10 Nov 2015 22:31:23 +0000 http://www.bioradiations.com/?p=7958 What is so noble about receiving an award? What if it is an Ig Nobel award? This article reviews what Ig Nobel awards are, how they are determined, and discusses some research topics that were awarded the Ig Nobel awards this year.

The post In Pursuit of (Ig) Nobility: The Ig Nobel Prize first appeared on Bio-Radiations.]]> 10 Tips and Tricks for Designing Multicolor Flow Cytometry Panels https://www.bioradiations.com/ten-tips-and-tricks-for-designing-multicolor-flow-cytometry-panels/ Tue, 10 Nov 2015 22:30:16 +0000 http://www.bioradiations.com/?p=7969 Multiplexing an assay comes with its own complications in addition to the benefits it affords. Designing multicolor panels for flow cytometry analysis entails several considerations for a successful outcome. Here is a list of tips that can help you succeed in setting up and running a multicolor flow cytometry panel.

The post 10 Tips and Tricks for Designing Multicolor Flow Cytometry Panels first appeared on Bio-Radiations.]]> How to Minimize Contamination in qPCR Experiments https://www.bioradiations.com/how-to-minimize-contamination-in-qpcr-experiments/ Tue, 10 Nov 2015 22:16:42 +0000 http://www.bioradiations.com/?p=7998 Are you using ethanol to prep your bench for qPCR? Watch this episode to find out why that might be a bad idea. We're sharing seven tips for minimizing contamination of qPCR experiments.

The post How to Minimize Contamination in qPCR Experiments first appeared on Bio-Radiations.]]> #6 What Factors Should You Consider for Chromatography Method Development https://www.bioradiations.com/6-what-factors-should-you-consider-for-chromatography-method-development/ Tue, 10 Nov 2015 22:13:49 +0000 http://www.bioradiations.com/?p=7967 Explore some of the key considerations for chromatography method development.

The post #6 What Factors Should You Consider for Chromatography Method Development first appeared on Bio-Radiations.]]> Why Elephants Rarely Get Cancer: It’s in Their Genes https://www.bioradiations.com/why-elephants-rarety-get-cancer-its-in-their-genes/ Tue, 10 Nov 2015 22:10:32 +0000 http://www.bioradiations.com/?p=7983 Why is cancer so rare in elephants? Scientists have observed this for decades, yet the explanation has eluded them until recently. Researchers at the University of Utah’s Huntsman Cancer Institute (HCI) and Arizona State University discovered elephants have extra copies of genes encoding p53, a tumor suppressor, and more robust apoptosis (programmed cell death) of damaged cells that may lead to cancer. According to the authors, these findings may aid future development of anti-cancer therapies in humans.

The post Why Elephants Rarely Get Cancer: It’s in Their Genes first appeared on Bio-Radiations.]]> The Cure for Cancer Could Come from…Malaria? https://www.bioradiations.com/the-cure-for-cancer-could-come-from-malaria-2/ Tue, 10 Nov 2015 22:10:30 +0000 http://www.bioradiations.com/?p=7989 Scientists have discovered that a protein commonly associated with malaria could one day lead to the cure for cancer. Found quite by accident, by researchers at the University of British Columbia and the University of Copenhagen studying why pregnant women are more susceptible to malaria, this protein binds to a particular type of sugar molecule that is found in the placenta and is prevalent in a high proportion of cancers. The researchers were not surprised by this finding, since cancers and the placenta both grow rapidly, often at the expense of other tissues.

The post The Cure for Cancer Could Come from…Malaria? first appeared on Bio-Radiations.]]> Crows Do Fear the Reaper https://www.bioradiations.com/crows-do-fear-the-reaper/ Tue, 10 Nov 2015 22:09:27 +0000 http://www.bioradiations.com/?p=7994 Crows have been shown to associate dead bodies of their own kind with danger, and are also known to remember the faces of individual humans who have threatened them.

The post Crows Do Fear the Reaper first appeared on Bio-Radiations.]]> Radiation Therapy for Skin Cancer: All Wrapped Up in a Bandage https://www.bioradiations.com/radiation-therapy-for-skin-cancer-all-wrapped-up-in-a-bandage/ Tue, 10 Nov 2015 21:51:04 +0000 http://www.bioradiations.com/?p=8059 Most of us think of bandages as a way to protect cuts and abrasions against infection. However, recent research shows that a novel radiotherapeutic bandage may help treat squamous cell carcinoma (SCC). A group of scientists from the University of North Texas System College of Pharmacy presented their findings at the American Association of Pharmaceutical Sciences Annual Meeting and Exposition.

The post Radiation Therapy for Skin Cancer: All Wrapped Up in a Bandage first appeared on Bio-Radiations.]]> Bioradiations October Edition https://www.bioradiations.com/bioradiations-october-edition/ Fri, 16 Oct 2015 22:34:24 +0000 http://www.bioradiations.com/?p=7937 See what new articles and videos are in the October edition of Bioradiations.

The post Bioradiations October Edition first appeared on Bio-Radiations.]]> What Academic Researchers Can Learn from Small Biotechs about Antibody Validation https://www.bioradiations.com/what-academic-researchers-can-learn-from-small-biotechs-about-antibody-validation/ Wed, 14 Oct 2015 00:36:45 +0000 http://www.bioradiations.com/?p=7819 Academic labs don't have the money to do full-scale validations of antibodies. What tricks can small biotechs teach them about validation? Find out.

The post What Academic Researchers Can Learn from Small Biotechs about Antibody Validation first appeared on Bio-Radiations.]]> A Semi-Automated Quantitative PCR Approach to Assess Differential Anti-Apoptotic Gene Expression Patterns Associated with Prostate Cancer Progression https://www.bioradiations.com/a-semi-automated-quantitative-pcr-approach-to-assess-differential-anti-apoptotic-gene-expression-patterns-associated-with-prostate-cancer-progression/ Wed, 14 Oct 2015 00:35:36 +0000 http://www.bioradiations.com/?p=7750 Gene expression analyses become complicated and extremely time consuming when large numbers of genes need to be analyzed. Here we present a semi-automated qPCR workflow with reduced setup and processing times using Bio-Rad's PrimePCR™ Panels and CFX Automation System II Plate Handler.

The post A Semi-Automated Quantitative PCR Approach to Assess Differential Anti-Apoptotic Gene Expression Patterns Associated with Prostate Cancer Progression first appeared on Bio-Radiations.]]> Protocol: Preparation of Cells for Flow Cytometry https://www.bioradiations.com/protocol-preparation-of-cells-for-flow-cytometry/ Wed, 14 Oct 2015 00:34:44 +0000 http://www.bioradiations.com/?p=7795 Harvesting of tissue culture cells could be challenging. Here is an easy protocol for efficient harvesting of cells for flow cytometry analysis.

The post Protocol: Preparation of Cells for Flow Cytometry first appeared on Bio-Radiations.]]> How to Design Primers for qPCR https://www.bioradiations.com/how-to-design-primers-for-qpcr/ Wed, 14 Oct 2015 00:33:11 +0000 http://www.bioradiations.com/?p=7843 How long should a qPCR primer be and what types of sequences should you avoid? In this episode we share eight tips and tricks for primer design for your qPCR experiments.

The post How to Design Primers for qPCR first appeared on Bio-Radiations.]]> #5 How Do You Choose the Right Hardware for Your Chromatography Application? https://www.bioradiations.com/5-how-do-you-choose-the-right-hardware-for-your-chromatography-application/ Wed, 14 Oct 2015 00:32:13 +0000 http://www.bioradiations.com/?p=7738 Which valves, pumps or detectors are optimal for your FPLC system? How do you achieve optimal gradient performance? Here are some tips.

The post #5 How Do You Choose the Right Hardware for Your Chromatography Application? first appeared on Bio-Radiations.]]> Scientists Image Metabolites Secreted by Single Cells https://www.bioradiations.com/scientists-image-metabolites-secreted-by-single-cells/ Wed, 14 Oct 2015 00:31:05 +0000 http://www.bioradiations.com/?p=7742 Bacteria secrete small molecules for purposes that range from communication to self-defense. These processes are extremely challenging to study in situ because it is nearly impossible to link secreted molecules to specific populations of bacteria let alone individual cells in their natural environment. By combining mass spectrometry-based imaging with fluorescence in situ hybridization (FISH) scientists at the Max Planck Institute for Chemical Ecology are able to image secretion patterns of antibiotics and map them onto the FISH profile of the bacterial population that underlies it.

The post Scientists Image Metabolites Secreted by Single Cells first appeared on Bio-Radiations.]]> Need an Implant? Just Print It Out https://www.bioradiations.com/need-an-implant-just-print-it-out/ Tue, 13 Oct 2015 18:15:11 +0000 http://www.bioradiations.com/?p=7930 Patients who need a life-saving implant may soon be able to get a customized one from a 3D printer. In the first procedure of its kind, surgeons replaced the sternum and a portion of the rib cage with a 3D-printed titanium sternum and rib implant. The procedure, performed in a 54-year-old Spanish man with a chest wall sarcoma, was reported in a recent issue of the European Journal of Cardio-Thoracic Surgery.

The post Need an Implant? Just Print It Out first appeared on Bio-Radiations.]]> Single Mutations Can Alter Ecosystems to Same Extent as Species Loss https://www.bioradiations.com/single-mutations-can-alter-ecosystems-to-same-extent-as-species-loss/ Tue, 13 Oct 2015 18:13:17 +0000 http://www.bioradiations.com/?p=7928 To date most research into what drives the composition of ecosystems has focused on macroevolution, factors such as extinction events, invasion of new species, or large shifts in population abundances. A study in Nature Communications now shows that microevolution — small changes within a single population — is an equally important yet hitherto overlooked shaper of multi-species communities.

The post Single Mutations Can Alter Ecosystems to Same Extent as Species Loss first appeared on Bio-Radiations.]]> Viruses Re-engineered to Do Good https://www.bioradiations.com/viruses-re-engineered-to-do-good/ Tue, 13 Oct 2015 18:09:22 +0000 http://www.bioradiations.com/?p=7925 Scientists at Stanford University recently repurposed viruses by removing their infectious machinery and turning them into vehicles for delivering therapies directly to cells. In their study, published in the Proceedings of the National Academy of Sciences, the scientists found a way to add molecular tags to the viral structure, which would enable them to target the viruses, and hence anything they are carrying, to a specific cell type.

The post Viruses Re-engineered to Do Good first appeared on Bio-Radiations.]]> A Non-Invasive Laser Doppler System Detects Malignant Melanoma https://www.bioradiations.com/a-non-invasive-laser-doppler-system-detects-malignant-melanoma/ Tue, 13 Oct 2015 18:05:28 +0000 http://www.bioradiations.com/?p=7923 A recent study showed that a novel laser Doppler system effectively distinguished skin malignant melanoma from noncancerous moles by detecting subtle differences in blood flow beneath the skin, which may aid in rapid and non-invasive screening of patients.

The post A Non-Invasive Laser Doppler System Detects Malignant Melanoma first appeared on Bio-Radiations.]]> A New Phage-engineering Platform Allows High-throughput Assembly of Hybrid Viral Genomes in Yeast https://www.bioradiations.com/a-new-phage-engineering-platform-allows-high-throughput-assembly-of-hybrid-viral-genomes-in-yeast/ Tue, 13 Oct 2015 18:02:39 +0000 http://www.bioradiations.com/?p=7921 Bacteriophages target bacteria with high specificity, a feature that has been exploited for biotechnology and therapeutics. That same exquisite specificity has, however, also been limiting — identifying or engineering phages with new specificities is often a prohibitively time consuming and laborious process. Scientists at MIT have now developed a yeast-based phage-engineering platform that overcomes these hurdles.

The post A New Phage-engineering Platform Allows High-throughput Assembly of Hybrid Viral Genomes in Yeast first appeared on Bio-Radiations.]]> Want to Lose Weight? Check with Your Gut Microbiome https://www.bioradiations.com/want-to-lose-weight-check-with-your-gut-microbiome/ Tue, 13 Oct 2015 17:54:28 +0000 http://www.bioradiations.com/?p=7919 Researchers at Chalmers University of Technology recently identified how intestinal bacteria interact during metabolism, paving the way to, among other things, possibly personalizing weight-loss diets. The systems biologists developed a computational algorithm that is able to predict how modifying a person’s diet will affect them based on the bacterial and microorganismal composition of their gut microbiome.

The post Want to Lose Weight? Check with Your Gut Microbiome first appeared on Bio-Radiations.]]> Melting Body Fat: Is it In Your Head? https://www.bioradiations.com/melting-body-fat-is-it-in-your-head/ Tue, 13 Oct 2015 17:52:10 +0000 http://www.bioradiations.com/?p=7916 Muscles may not be the only organ that burns body fat. A recent study published in Cell showed that directly stimulating neurons in adipose (fat) tissue may induce breakdown, providing insight on novel therapies to combat obesity.

The post Melting Body Fat: Is it In Your Head? first appeared on Bio-Radiations.]]> Droplet Digital™ Applications: Phasing of Genetic Variants for CFTR Haplotyping https://www.bioradiations.com/droplet-digital-applications-phasing-of-genetic-variants-for-cftr-haplotyping/ Fri, 09 Oct 2015 19:01:29 +0000 http://www.bioradiations.com/?p=7871 In this webinar, Dr. Jack Regan describes how the Droplet Digital PCR (ddPCR™) System can be used to determine whether genomic sequences are physically linked…  

The post Droplet Digital™ Applications: Phasing of Genetic Variants for CFTR Haplotyping first appeared on Bio-Radiations.]]> Optimizing to Automation: The Advantages of Multidimensional (Multi-D) Chromatography https://www.bioradiations.com/optimizing-to-automation-the-advantages-of-multidimensional-multi-d-chromatography/ Fri, 09 Oct 2015 17:54:36 +0000 http://www.bioradiations.com/?p=7854 Philip Chapman of Bio-Rad Laboratories discusses the advantages of automated multidimensional (Multi-D) chromatography compared to traditional multistep purification strategies.  

The post Optimizing to Automation: The Advantages of Multidimensional (Multi-D) Chromatography first appeared on Bio-Radiations.]]> Can Cancer Cells be Programmed Back to Normal? https://www.bioradiations.com/can-cancer-cells-be-programmed-back-to-normal/ Mon, 14 Sep 2015 00:30:01 +0000 http://www.bioradiations.com/?p=7740 In a recent study published in Nature Cell Biology, researchers at the Mayo Clinic described a way in which cancer cells could potentially be programmed back into normal cells. Their findings were stimulated by the fact that proteins that hold cells together, or adhesion proteins, interact with the Microprocessor complex, which mediates the production of microRNAs (miRNAs).

The post Can Cancer Cells be Programmed Back to Normal? first appeared on Bio-Radiations.]]> Bioradiations September Edition https://www.bioradiations.com/bioradiations-september-edition/ Fri, 11 Sep 2015 00:30:16 +0000 http://www.bioradiations.com/?p=7596 See what new articles and videos are in the September edition of Bioradiations.

The post Bioradiations September Edition first appeared on Bio-Radiations.]]> Four Tips for Developing a Sample Prep Protocol for SDS-PAGE or Chromatography https://www.bioradiations.com/four-tips-for-developing-a-sample-prep-protocol-for-sds-page-or-chromatography/ Fri, 04 Sep 2015 17:53:25 +0000 http://www.bioradiations.com/?p=7531 From making sure that your lysis buffer is compatible with your protein quantitation method to advice on when you do and don't have to worry about proteases, we share helpful tips for developing a sample preparation protocol.

The post Four Tips for Developing a Sample Prep Protocol for SDS-PAGE or Chromatography first appeared on Bio-Radiations.]]> Five Tips for Picking the Right Cell Disruption Method for Protein Analysis https://www.bioradiations.com/five-tips-for-picking-the-right-cell-disruption-method-for-protein-analysis/ Fri, 04 Sep 2015 17:51:15 +0000 http://www.bioradiations.com/?p=7529 Sonicator or freeze-thaw? Detergent or enzymatic lysis? Find out how to pick the right cell disruption method for your experiment.

The post Five Tips for Picking the Right Cell Disruption Method for Protein Analysis first appeared on Bio-Radiations.]]> A Pungent Play in the Game of Cat and Mouse https://www.bioradiations.com/a-pungent-play-in-the-game-of-cat-and-mouse/ Fri, 04 Sep 2015 00:48:07 +0000 http://www.bioradiations.com/?p=7429 It was discovered a few years ago that the parasite Toxoplasma gondii has the diabolical effect of short circuiting the fear of cats in the brains of mice, making them easy picking. Researchers at the A.N. Severtov Institute of Ecology and Evolution in Moscow now show that a more readily abundant substance has a similar effect: the cats’ own urine.

The post A Pungent Play in the Game of Cat and Mouse first appeared on Bio-Radiations.]]> Breastmilk and Human Microbiome Development https://www.bioradiations.com/breastmilk-and-human-microbiome-development/ Fri, 04 Sep 2015 00:47:45 +0000 http://www.bioradiations.com/?p=7526 The importance of gut microbes to well-being is one of the hottest topics in health. Another is the benefits of breastfeeding. It’s popularly assumed that breastmilk supports a healthy microbiome in infants, but what does the research show? A new review article in Science outlines what we know and don’t know about how this support may work.

The post Breastmilk and Human Microbiome Development first appeared on Bio-Radiations.]]> Revealing the Secrets of How Cancers Could Occur https://www.bioradiations.com/revealing-the-secrets-of-how-cancers-could-occur/ Fri, 04 Sep 2015 00:45:03 +0000 http://www.bioradiations.com/?p=7524 In an article recently published in Nature Communications, researchers at the University of Toronto, along with scientists at Mount Sinai Hospital, demonstrated how what they call DNA ambulances work. These DNA ambulances transport severely injured DNA to specialized locations, or hospitals, within the cell to be repaired. In addition to the mode of transport, the researchers also found the road traveled by these healing transporters.

The post Revealing the Secrets of How Cancers Could Occur first appeared on Bio-Radiations.]]> From Child to Mother: Fetal Cells in Maternal Tissue May Impact Maternal Health After Childbirth https://www.bioradiations.com/from-child-to-mother-fetal-cells-in-maternal-tissue-may-impact-maternal/ Fri, 04 Sep 2015 00:38:55 +0000 http://www.bioradiations.com/?p=7522 Most of us probably know that the mother’s health is important for the well-being of their developing fetus. However, a recent study published in BioEssays suggests that the fetus may also affect the health of its mother — even after the pregnancy is over. The study showed that fetal cells migrate from the placenta and reside in several parts of the mother’s body, where they may exert benefits (such as improved milk production and thermoregulation), harms (such as autoimmune diseases and cancer), or have no effect (such as their presence in the lung).

The post From Child to Mother: Fetal Cells in Maternal Tissue May Impact Maternal Health After Childbirth first appeared on Bio-Radiations.]]> A New Way to PCR Could Get You Results in Minutes Instead of Hours https://www.bioradiations.com/a-new-way-to-pcr-could-get-you-results-in-minutes-instead-of-hours/ Fri, 04 Sep 2015 00:36:59 +0000 http://www.bioradiations.com/?p=7520 Whether you are using PCR to clone, assess gene expression, or diagnose disease, modern science is difficult to imagine without PCR. Scientists at UC Berkeley have rethought how we heat and cool samples during PCR, shortening a process that once took hours to minutes.

The post A New Way to PCR Could Get You Results in Minutes Instead of Hours first appeared on Bio-Radiations.]]> #4 How Do You Listen to Your FPLC UV Detector? https://www.bioradiations.com/4-how-do-you-listen-to-your-fplc-uv-detector/ Fri, 04 Sep 2015 00:24:01 +0000 http://www.bioradiations.com/?p=7517 Here are some tips to fix random spikes in your peaks and pulsations in your gradient.

The post #4 How Do You Listen to Your FPLC UV Detector? first appeared on Bio-Radiations.]]> Trends in Antibody Generation Techniques — the Fully Synthetic Human Combinatorial Antibody Library (HuCAL®) Technology https://www.bioradiations.com/trends-in-antibody-generation-techniques-the-fully-synthetic-human-combinatorial-antibody-library-hucal-technology/ Fri, 04 Sep 2015 00:01:25 +0000 http://www.bioradiations.com/?p=7501 From using nonspecific polyclonal antibodies to developing monoclonal antibodies from immortalized antibody-producing cells to antibody engineering and generation of antibodies using an animal-free library with billions of antibody fragments to choose from, the antibody technology has seen tremendous advances. Explore where this journey started and what the current trends are and what the human combinatorial antibody library (HuCAL) technology destination has to offer!

The post Trends in Antibody Generation Techniques — the Fully Synthetic Human Combinatorial Antibody Library (HuCAL®) Technology first appeared on Bio-Radiations.]]> Bio-Rad’s Multi-D Method Addresses Unmet Chromatography Needs https://www.bioradiations.com/bio-rads-multi-d-method-addresses-unmet-chromatography-needs/ Thu, 03 Sep 2015 00:14:06 +0000 http://www.bioradiations.com/?p=7514 Some of the frustrations in protein purification stem from unmet chromatography needs, such as automation, productivity, throughput, and reproducibility among others. Bio-Rad’s new Multi-D chromatography method addresses these and provides solutions to faster, efficient and more reliable protein purification.

The post Bio-Rad’s Multi-D Method Addresses Unmet Chromatography Needs first appeared on Bio-Radiations.]]> Ultra-Sensitive Quantification of Genome Editing Events Using Droplet Digital PCR https://www.bioradiations.com/ultra-sensitive-quantification-of-genome-editing-events-using-droplet-digital-pcr/ Tue, 25 Aug 2015 18:52:06 +0000 http://www.bioradiations.com/?p=7437 Genome editing using tools such as CRISPR/Cas9 and TALEN has gained enormous popularity in recent days due to their ability to create precise deletions or insertions. However when the frequency of these gene edits are really small, an ultrasensitive method like ddPCR™ is needed for accurate quantification. This technote explains how Droplet Digital™ PCR is used for the detection of HDR and NHEJ alleles generated using CRISPR/Cas9 and TALEN editing systems.

The post Ultra-Sensitive Quantification of Genome Editing Events Using Droplet Digital PCR first appeared on Bio-Radiations.]]> #3 How Do You Care for Your Chromatography System? https://www.bioradiations.com/3-how-do-you-care-for-your-chromatography-system/ Thu, 20 Aug 2015 23:25:02 +0000 http://www.bioradiations.com/?p=7427 Here are some handy tips for maintaining your chromatography columns in great shape.

The post #3 How Do You Care for Your Chromatography System? first appeared on Bio-Radiations.]]> Bioradiations August Edition https://www.bioradiations.com/bioradiations-august-edition/ Wed, 12 Aug 2015 23:10:16 +0000 http://www.bioradiations.com/?p=7366 See what new articles and videos are in the August edition of Bioradiations.

The post Bioradiations August Edition first appeared on Bio-Radiations.]]> CRISPR: The Hopes, the Fears, and the Biology https://www.bioradiations.com/crispr-the-hopes-the-fears-and-the-biology/ Tue, 11 Aug 2015 22:49:16 +0000 http://www.bioradiations.com/?p=7354 Few discoveries have changed the pace of discovery and the types of questions we can ask as drastically and quickly as CRISPR-based genome editing. But where did CRISPR come from? How was it adapted to become such a game-changing genome editing tool? And why are some of its greatest proponents alarmed?

The post CRISPR: The Hopes, the Fears, and the Biology first appeared on Bio-Radiations.]]> Four Tips for Handcasting SDS-PAGE Gels https://www.bioradiations.com/four-tips-for-handcasting-sds-page-gels/ Tue, 11 Aug 2015 22:30:00 +0000 http://www.bioradiations.com/?p=7349 Watch this episode of LabNotes for helpful tips like how to pour the stacking and resolving gel at the same time.

The post Four Tips for Handcasting SDS-PAGE Gels first appeared on Bio-Radiations.]]> Four Tips for Running the Perfect SDS-PAGE Gel https://www.bioradiations.com/four-tips-for-running-the-perfect-sds-page-gel/ Tue, 11 Aug 2015 22:25:07 +0000 http://www.bioradiations.com/?p=7344 We share five simple tricks that address common gel running woes, from samples that float out of wells to sample buffers that turn yellow.

The post Four Tips for Running the Perfect SDS-PAGE Gel first appeared on Bio-Radiations.]]> The Antibody Challenge: Bio-Rad’s Precise Solution https://www.bioradiations.com/the-antibody-challenge-bio-rads-precise-solution/ Tue, 11 Aug 2015 22:15:36 +0000 http://www.bioradiations.com/?p=7323 Why western blotting, a technique used in every day research, is still not as straightforward as it seems? Well, it’s mainly because of the antibodies. They may not work in all samples, a lot of standardization needs to be done and there are several other unknowns. Bio-Rad, the leader in western blotting, has addressed these concerns in its newest line of antibodies, the PrecisionAb™ Antibodies. See the challenges and the Bio-Rad solution.

The post The Antibody Challenge: Bio-Rad’s Precise Solution first appeared on Bio-Radiations.]]> TOR Pathway Allows Yeast to Optimize Their Genome in Response to Environmental cChanges https://www.bioradiations.com/tor-pathway-allows-yeast-to-optimize-their-genome-in-response-to-environmental-cchanges/ Tue, 11 Aug 2015 22:10:49 +0000 http://www.bioradiations.com/?p=7282 It is generally accepted that changes to a cell’s genome are driven by random mutation. Changes that confer a growth advantage become established in a population through the process of natural selection. A study published in PNAS suggests that cells can play a more active role in the evolution of their genomes — in response to caloric excess the TOR pathway in budding yeast initiates an expansion of the number of ribosomal genes.

The post TOR Pathway Allows Yeast to Optimize Their Genome in Response to Environmental cChanges first appeared on Bio-Radiations.]]> Cannabis-Based Therapies Without the Side Effects: A New Treatment Paradigm for Cancer? https://www.bioradiations.com/cannabis-based-therapies-without-the-side-effects-a-new-treatment-paradigm-for-cancer/ Tue, 11 Aug 2015 22:10:06 +0000 http://www.bioradiations.com/?p=7280 Recently, scientists at the University of East Anglia (UEA) and the University Pompeu Fabra in Barcelona, Spain identified that inhibition of a signaling pathway in the brain, involving both a cannabinoid receptor and a serotonin receptor, prevented the undesirable side effects of THC while maintaining its therapeutic potential.

The post Cannabis-Based Therapies Without the Side Effects: A New Treatment Paradigm for Cancer? first appeared on Bio-Radiations.]]> Aliens in Our Midst https://www.bioradiations.com/aliens-in-our-midst/ Tue, 11 Aug 2015 22:08:42 +0000 http://www.bioradiations.com/?p=7276 As we seek out organic compounds and water on the surface of Mars, ponder the potential for hidden oceans on Europa, and discover new Earth-like exoplanets, the promise of astrobiology — namely, alien life in some form — seems closer than ever to reality. Yet what if aliens are already here on Earth, not as otherworldly visitors but alternative organisms, separately evolved and existing in a shadow biosphere?

The post Aliens in Our Midst first appeared on Bio-Radiations.]]> Our Cells Unwittingly Support Viral Entry and Infection https://www.bioradiations.com/our-cells-unwittingly-support-viral-entry-and-infection/ Tue, 11 Aug 2015 22:07:01 +0000 http://www.bioradiations.com/?p=7274 Human adenoviruses comprise 25% of all gene therapy clinical trials due, in part, to their ability to infect both dividing and nondividing cells with persistent expression. Recently, researchers at the University of Zurich demonstrated how these viruses gain entry into our cells — and it’s quite sneaky!

The post Our Cells Unwittingly Support Viral Entry and Infection first appeared on Bio-Radiations.]]> How the Microbiome of Soil Affects Global Warming https://www.bioradiations.com/how-the-microbiome-of-soil-affects-global-warming/ Tue, 11 Aug 2015 22:06:26 +0000 http://www.bioradiations.com/?p=7272 The ground beneath our feet is teeming with microbial life—approximately 10 billion organisms in an average handful of soil. Now, thanks to modern genetic sequencing methods, researchers are getting surprising insights into the vibrant world of dirt.

The post How the Microbiome of Soil Affects Global Warming first appeared on Bio-Radiations.]]> Gut Microbiome May Predict Progression of Colon Cancer https://www.bioradiations.com/gut-microbiome-may-predict-progression-of-colon-cancer/ Tue, 11 Aug 2015 22:05:00 +0000 http://www.bioradiations.com/?p=7269 The gut microbiome has been linked to a wide range of health indicators over recent years and, according to a recent study published in Genome Medicine, may help predict prognosis of patients with colon cancer. In this study, the microenvironment of colon cancer tumors had an abundance of Fusobacterium and Providencia species, perhaps identifying a microbiome signature that predicts patient prognosis.

The post Gut Microbiome May Predict Progression of Colon Cancer first appeared on Bio-Radiations.]]> The Youth Factor: It’s in Your Blood https://www.bioradiations.com/the-youth-factor-its-in-your-blood/ Tue, 11 Aug 2015 21:57:57 +0000 http://www.bioradiations.com/?p=7319 Finding ways to slow or reverse aging intrigues scientists and non-scientists alike. In recent studies, merging the circulatory systems of young and old mice rejuvenated skeletal muscle, brain, and cardiac cells, indicating the presence of a systemic “anti-aging factor” initially thought to be growth differentiation factor 11 (GDF11). However, a recent publication showed that GDF11 actually promotes aging, emphasizing the need for further integrative analysis of the aging process.

The post The Youth Factor: It’s in Your Blood first appeared on Bio-Radiations.]]> #2 How Do You Best Prepare Your Columns for Chromatography? https://www.bioradiations.com/2-how-do-you-best-prepare-your-columns-for-chromatography/ Tue, 11 Aug 2015 18:59:53 +0000 http://www.bioradiations.com/?p=7310 Here are some tips for attaching, filling or running your chromatography columns.

The post #2 How Do You Best Prepare Your Columns for Chromatography? first appeared on Bio-Radiations.]]> Get Published! 10 Tips for Getting Published Successfully https://www.bioradiations.com/get-published-10-tips-for-getting-published-successfully/ Tue, 11 Aug 2015 09:34:26 +0000 http://www.bioradiations.com/?p=7284 When you have finished all your experiments and have the paper ready in your head, you need to consider several factors before sending it for publication. Here are some tips that Bio-Rad’s PrimePCR™ PCR Primers and Assays group put together for getting the paper published successfully.

The post Get Published! 10 Tips for Getting Published Successfully first appeared on Bio-Radiations.]]> Bioradiations July Edition https://www.bioradiations.com/bioradiations-july-edition/ Thu, 16 Jul 2015 18:09:26 +0000 http://www.bioradiations.com/?p=7259 See what new articles and videos are in the July edition of Bioradiations.

The post Bioradiations July Edition first appeared on Bio-Radiations.]]> Choosing the Right Percentage Gel https://www.bioradiations.com/choosing-the-right-percentage-gel/ Wed, 15 Jul 2015 00:02:07 +0000 http://www.bioradiations.com/?p=7141 Unsure about what percentage SDS-PAGE gel to pick for your experiment? We share four tips that make picking the perfect gel a piece of cake.

The post Choosing the Right Percentage Gel first appeared on Bio-Radiations.]]> Automated Multi-D Chromatography on the NGC™ System https://www.bioradiations.com/automated-multi-d-chromatography-on-the-ngc-system/ Wed, 15 Jul 2015 00:01:04 +0000 http://www.bioradiations.com/?p=7253 What is Multidimensional (Multi-D) chromatography? Here is an animated explanation of how Multi-D chromatography works and what its advantages are over traditional chromatography.

The post Automated Multi-D Chromatography on the NGC™ System first appeared on Bio-Radiations.]]> Next Generation Computers: Transforming Cells into Autonomous Computing Devices https://www.bioradiations.com/next-generation-computers-transforming-cells-into-autonomous-computing-devices/ Tue, 14 Jul 2015 23:13:50 +0000 http://www.bioradiations.com/?p=7128 What do computers and cells have in common? Can cells function as computers or recording devices? Can a bacterial computer be built? Synthetic biologists have recently developed a platform called SCRIBE (Synthetic Cellular Recorders Integrating Biological Events) to convert genomic DNA into a “tape” for recording and memorizing information. This article explores many interesting aspects of autonomous cellular memory and the potential applications of such living computational systems.

The post Next Generation Computers: Transforming Cells into Autonomous Computing Devices first appeared on Bio-Radiations.]]> What’s in An Instrument Nickname? https://www.bioradiations.com/whats-in-an-instrument-nickname/ Tue, 14 Jul 2015 23:12:58 +0000 http://www.bioradiations.com/?p=7237 Researchers love their lab instruments…and sometimes hate them too. Out of such complex human-machine relationships come nicknames, many many nicknames. Read on to explore the ways scientists name their instruments, the names they choose, and contribute your favorite instrument nickname!

The post What’s in An Instrument Nickname? first appeared on Bio-Radiations.]]> Six Things You Might Not Know About Bio-Rad’s Food Science Division (FSD) https://www.bioradiations.com/six-things-you-might-not-know-about-bio-rads-food-science-division-fsd/ Tue, 14 Jul 2015 22:51:29 +0000 http://www.bioradiations.com/?p=7228 What’s the science that keeps your food safe? Find out with an inside look at Bio-Rad’s Food Science Division, and discover how techniques from the research lab are applied every day to guard what you eat.

The post Six Things You Might Not Know About Bio-Rad’s Food Science Division (FSD) first appeared on Bio-Radiations.]]> Advantages of Multidimensional (Multi-D) Chromatography Using the NGC™ Chromatography System over Traditional Sequential Chromatography https://www.bioradiations.com/advantages-of-multidimensional-multi-d-chromatography-using-the-ngc-chromatography-system-over-traditional-sequential-chromatography/ Tue, 14 Jul 2015 22:47:24 +0000 http://www.bioradiations.com/?p=7212 Traditional sequential chromatography typically requires intensive hands-on user manipulation, which could hinder the reproducibility of protein purification runs. Multi-D chromatography aims to improve this process while maintaining the efficacy of the traditional workflow.

The post Advantages of Multidimensional (Multi-D) Chromatography Using the NGC™ Chromatography System over Traditional Sequential Chromatography first appeared on Bio-Radiations.]]> Minimizing Protein Degradation https://www.bioradiations.com/minimizing-protein-degradation/ Tue, 14 Jul 2015 21:12:41 +0000 http://www.bioradiations.com/?p=7137 In this episode, we share tips and tricks for minimizing protein degradation during cell lysis and sample preparation.

The post Minimizing Protein Degradation first appeared on Bio-Radiations.]]> Mitochondria and Plastids Evolved in Miniscule Plankton to Form Eye-like Structure https://www.bioradiations.com/mitochondria-and-plastids-evolved-in-miniscule-plankton-to-form-eye-like-structure/ Tue, 14 Jul 2015 20:53:11 +0000 http://www.bioradiations.com/?p=7166 An eye-like structure called the ocelloid, found in warnowiid dinoflagellates, has long puzzled biologists. This structure resembles the eye of higher organisms to such a degree, that is was first assumed to be part of an animal the warnowiid had eaten. Now this small eukaryotic plankton has surprised scientists again – the warnowiid eye’s building blocks are mitochondria and plastids.

The post Mitochondria and Plastids Evolved in Miniscule Plankton to Form Eye-like Structure first appeared on Bio-Radiations.]]> A Fifth DNA Base? https://www.bioradiations.com/a-fifth-dna-base/ Tue, 14 Jul 2015 20:51:55 +0000 http://www.bioradiations.com/?p=7164 A ubiquitous but rare DNA base, previously thought to be a transitional chemical modification of cytosine, has now been shown to be stably incorporated in mammalian DNA. 5-formylcytosine (5fC) is found in all tissues, with the highest levels being found in the brain. Its exact function is unknown, but its physical position in the genome suggests that it plays a key role in gene expression.

The post A Fifth DNA Base? first appeared on Bio-Radiations.]]> Disrupting the Regeneration of Brain Tumors https://www.bioradiations.com/disrupting-the-regeneration-of-brain-tumors/ Tue, 14 Jul 2015 20:50:43 +0000 http://www.bioradiations.com/?p=7162 Brain tumors such as glioblastomas are exceptionally difficult to treat since, irrespective of how they were treated, they find a way to regenerate. This ability can be attributed to cancer stem cells that circumvent treatment and trigger the expansion of new tumor cells. Recently, scientists at Washington University School of Medicine in St. Louis discovered a way to disrupt the regeneration of brain tumors by disrupting a key player in the brain tumor stem cell maintenance process.

The post Disrupting the Regeneration of Brain Tumors first appeared on Bio-Radiations.]]> Growing Mini-Breasts for Cancer Research https://www.bioradiations.com/growing-mini-breasts-for-cancer-research/ Tue, 14 Jul 2015 20:49:41 +0000 http://www.bioradiations.com/?p=7160 Researchers often use two-dimensional cell culture studies to study cancer biology. However, a recent group of researchers, led by Dr. Christina Scheel at the German Research Center for Environmental Health, has advanced this experimental model by creating three-dimensional “mini-breasts”— organoid structures derived from human breast epithelial cells.

The post Growing Mini-Breasts for Cancer Research first appeared on Bio-Radiations.]]> Paleontology Gets a Shot of Dinosaur Protein https://www.bioradiations.com/paleontology-gets-a-shot-of-dinosaur-protein/ Tue, 14 Jul 2015 20:48:21 +0000 http://www.bioradiations.com/?p=7158 In all but the best-preserved fossils, researchers have assumed that intact soft tissues did not survive longer than about 4 million years. A few protein fragments might persist; however, full proteinaceous structures that could tell us about soft tissues and animal physiology would not.

The post Paleontology Gets a Shot of Dinosaur Protein first appeared on Bio-Radiations.]]> Social Amoeba Answer Age-Old Question: Do Cheaters Always Win? https://www.bioradiations.com/social-amoeba-answer-age-old-question-do-cheaters-always-win/ Tue, 14 Jul 2015 20:46:28 +0000 http://www.bioradiations.com/?p=7156 The social amoeba Dictyostelium discoideum has two life stages. When nutrients are plentiful, Dicty live as individuals but when starvation sets in, thousands of Dicty come together to form a fruiting body. This fruiting body is made up of a stalk that holds up a ball of spores, and this is where the cheating comes in — only cells that make it to the top of the fruiting body and become a spore get to live and pass on their genetic information.

The post Social Amoeba Answer Age-Old Question: Do Cheaters Always Win? first appeared on Bio-Radiations.]]> A “Google Maps” Approach to Navigate the Cancer Genome https://www.bioradiations.com/a-google-maps-approach-to-navigate-the-cancer-genome/ Tue, 14 Jul 2015 20:44:28 +0000 http://www.bioradiations.com/?p=7154 Researchers have made significant progress recently in the development of personalized cancer genomics and therapies through analysis of characteristic variations of single base pairs and chromosomes. Recently, researchers at the University of Wisconsin-Madison used an integrated system of DNA sequencing and optical mapping (a single-molecule, whole-genome analysis system) to identify both small- and large-scale genetic variations over time in a patient with multiple myeloma. Their findings were published on June 8, 2015 in the Proceedings of the National Academy of Sciences.

The post A “Google Maps” Approach to Navigate the Cancer Genome first appeared on Bio-Radiations.]]> #1 How Do You Best Prepare Your Protein Samples for Purification? https://www.bioradiations.com/1-how-do-you-best-prepare-your-protein-samples-for-purification/ Tue, 14 Jul 2015 18:38:00 +0000 http://www.bioradiations.com/?p=7149 Here are some tips to clarify your lysate, filter proteins, and to achieve optimal binding of your proteins.

The post #1 How Do You Best Prepare Your Protein Samples for Purification? first appeared on Bio-Radiations.]]> High Quality Gene Expression from Precious Samples — A Simplified Workflow https://www.bioradiations.com/high-quality-gene-expression-from-precious-samples-a-simplified-workflow/ Thu, 11 Jun 2015 17:45:48 +0000 http://www.bioradiations.com/?p=7099 Obtaining enough starting material to complete multiple gene expression experiments can be challenging when working with precious and limited samples. Find out how Mark Kibschull of the Lunenfeld-Tanenbaum Research Institute in Toronto determines pluripotency and differentiation of limited human embryonic stem cells using Bio-Rad's SingleShot Cell Lysis Kit, PreAmp Supermix, and PrimePCR validated primer panels. This simplified workflow allows for the gene expression analysis of hundreds of genes from as little as 100 human embryonic stem cells, and without the cumbersome step of isolating and purifying RNA.

The post High Quality Gene Expression from Precious Samples
— A Simplified Workflow first appeared on Bio-Radiations.]]> Bioradiations June Edition https://www.bioradiations.com/bioradiations-june-edition/ Wed, 10 Jun 2015 22:56:27 +0000 http://www.bioradiations.com/?p=7115 Get a quick look to see what new articles and videos are in the June edition.

The post Bioradiations June Edition first appeared on Bio-Radiations.]]> Too Many Questions, Too Little Sample: Developing a Real-Time PCR Workflow for Monitoring Gene Expression in Limited Samples https://www.bioradiations.com/too-many-questions-too-little-sample-developing-a-real-time-pcr-workflow-for-monitoring-gene-expression-in-limited-samples/ Tue, 09 Jun 2015 22:42:57 +0000 http://www.bioradiations.com/?p=7042 Traditional real-time PCR workflows often limit the number of genes that can be studied, especially when sample amounts are small. A new workflow now allows Mark Kibschull of the Lunenfeld-Tanenbaum Research Institute in Toronto to track the expression of hundreds of genes using a fraction of the starting material required by traditional methods.

The post Too Many Questions, Too Little Sample: Developing a Real-Time PCR Workflow for Monitoring Gene Expression in Limited Samples first appeared on Bio-Radiations.]]> 7 Tips for Choosing the Right Antibody for Western Blotting https://www.bioradiations.com/7-tips-for-choosing-the-right-antibody-for-western-blotting/ Tue, 09 Jun 2015 22:40:56 +0000 http://www.bioradiations.com/?p=7075 Which antibody is the best to obtain a definitive and conclusive answer for my questions? How do I choose the best antibody for my purpose? These are standard questions that scientists ask time and time again. Here are a few tips to choose the most appropriate antibody for your specific application.

The post 7 Tips for Choosing the Right Antibody for Western Blotting first appeared on Bio-Radiations.]]> Adding Another Dimension to Cancer Research: Three-dimensional Printing of Cancer Cells https://www.bioradiations.com/adding-another-dimension-to-cancer-research-three-dimensional-printing-of-cancer-cells/ Tue, 09 Jun 2015 20:38:50 +0000 http://www.bioradiations.com/?p=7096 Traditionally, researchers have relied on two-dimensional single-layer cell culture to analyze the physiological characteristics of tumor cells and their response to anti-cancer therapies. However, an international team of scientists recently used a three-dimensional (3D) printer to construct a three-dimensional (3D) model of a cancerous tumor, which may provide a more realistic representation of the tumor’s microenvironment and thus improve knowledge on how tumors develop, grow, and spread.

The post Adding Another Dimension to Cancer Research: Three-dimensional Printing of Cancer Cells first appeared on Bio-Radiations.]]> A New Player in the Fight against Ebola https://www.bioradiations.com/a-new-player-in-the-fight-against-ebola/ Tue, 09 Jun 2015 18:34:01 +0000 http://www.bioradiations.com/?p=7069 An international team of scientists recently identified the molecular factor that the Ebola virus attaches to in order to gain entry into host cells. The host protein, Neimann-Pick C1 (NPC1), is embedded in the membrane of lysosomes and allows the virus to gain entry to the cytoplasm, where the productive infection occurs. The study, which expanded upon findings from an earlier in vitro study, was carried out in NPC1 wild type, carrier, and knockout mice.

The post A New Player in the Fight against Ebola first appeared on Bio-Radiations.]]> With Potential Illicit Yeast Strains, Biology’s Breaking Bad Has Arrived https://www.bioradiations.com/with-potential-illicit-yeast-strains-biologys-breaking-bad-has-arrived/ Tue, 09 Jun 2015 18:28:28 +0000 http://www.bioradiations.com/?p=7064 Another week, another warning about the growing power and peril of genomic modification. Last time, the topic was using the CRISPR/Cas9 gene editing method to alter the human germ line (a group of researchers promptly published on how they did just this). Now, a new commentary in Nature takes up the implications of using modified yeast to produce controlled substances such as opiates.

The post With Potential Illicit Yeast Strains, Biology’s Breaking Bad Has Arrived first appeared on Bio-Radiations.]]> Cyclic di-GMP Acts as a Cyclin-like Molecule to Control Cell Cycle Progression in Bacteria https://www.bioradiations.com/cyclic-di-gmp-acts-as-a-cyclin-like-molecule-to-control-cell-cycle-progression-in-bacteria/ Tue, 09 Jun 2015 18:21:54 +0000 http://www.bioradiations.com/?p=7061 Cell division is a complicated process that requires not only cell growth and division but also faithful replication of the mother cell genome. These processes need to be timed appropriately to end up with two viable daughter cells. In eukaryotic cells cell cycle progression is controlled by oscillating proteins called cyclins that bind and activate cyclin-dependent kinases (Cdks).

The post Cyclic di-GMP Acts as a Cyclin-like Molecule to Control Cell Cycle Progression in Bacteria first appeared on Bio-Radiations.]]> Putting Cells “to Sleep”: a Novel, Gentler Way to Treat Cancer https://www.bioradiations.com/putting-cells-to-sleep-a-novel-gentler-way-to-treat-cancer/ Tue, 09 Jun 2015 18:20:29 +0000 http://www.bioradiations.com/?p=7058 Traditional treatment for cancer often focuses on killing the malignant cells through radiotherapy or chemotherapy. However, researchers at Oregon State University are investigating the use of pactamycin analogs to inhibit proliferation and induce senescence of head and neck cancer cells — essentially putting them “to sleep” rather than killing them directly.

The post Putting Cells “to Sleep”: a Novel, Gentler Way to Treat Cancer first appeared on Bio-Radiations.]]> Researcher Profile: Suzanne Topalian Pioneers PD-1 Inhibitor Drug to Treat Advanced Cancer https://www.bioradiations.com/researcher-profile-suzanne-topalian-pioneers-pd-1-inhibitor-drug-to-treat-advanced-cancer/ Wed, 27 May 2015 18:01:57 +0000 http://www.bioradiations.com/?p=6476 Suzanne Topalian was recognized in 2014 by Nature as one of “Ten People Who Mattered This Year” for her contributions in pioneering nivolumab, a programmed cell death protein-1 (PD-1) inhibitor that was recently approved in Japan for advanced melanoma treatment and that shifts the paradigm for a wide range of cancer therapies.

The post Researcher Profile: Suzanne Topalian Pioneers PD-1 Inhibitor Drug to Treat Advanced Cancer first appeared on Bio-Radiations.]]> Teaching Inquiry in the Classroom https://www.bioradiations.com/teaching-inquiry-in-the-classroom/ Wed, 20 May 2015 23:14:42 +0000 http://www.bioradiations.com/?p=7013 A look inside Julianne Zedalis' classroom at The Bishop's School in San Diego, CA, gives a glimpse of how a master teacher implements inquiry in her classroom. Bio-Rad's ThINQ! Investigations enable instructors to guide students through real-world inquiry investigations. These labs meet the revised College Board curriculum requirements and are aligned to AP Biology Big Ideas; they are designed to connect inquiry to NGSS and college curricula, encouraging students to think like real scientists.

The post Teaching Inquiry in the Classroom first appeared on Bio-Radiations.]]> Cell Imaging Simplified – the ZOE™ Fluorescent Cell Imager https://www.bioradiations.com/cell-imaging-simplified-the-zoe-fluorescent-cell-imager/ Wed, 20 May 2015 22:41:58 +0000 http://www.bioradiations.com/?p=7009 The ZOE fluorescent cell imager features an integrated digital camera and touch-screen interface that makes brightfield and multichannel fluorescence microscopy simple, fast, and intuitive. An easy-to-use alternative to the traditional inverted microscope, ZOE eliminates the complexities of multichannel fluorescence imaging by combining the ease of use of a personal tablet and the power of an inverted microscope.

The post Cell Imaging Simplified – the ZOE™ Fluorescent Cell Imager first appeared on Bio-Radiations.]]> 3 Moments of Inquiry with Bio-Rad’s pGLO™ Transformation and Inquiry for AP Biology: A ThINQ! Investigation Kit https://www.bioradiations.com/3-moments-of-inquiry-with-bio-rads-pglo-transformation-and-inquiry-for-ap-biology-a-thinq-investigation-kit/ Tue, 19 May 2015 23:15:07 +0000 http://www.bioradiations.com/?p=6512 “Inquiry-based” is the new buzzphrase in science education. But what do these learning practices look like on the ground? Visit one high school in Southern California and find out about this new approach to teaching science.

The post 3 Moments of Inquiry with Bio-Rad’s pGLO™ Transformation and Inquiry for AP Biology: A ThINQ! Investigation Kit first appeared on Bio-Radiations.]]> Introducing the ZOE™ Fluorescent Cell Imager for Routine Cell Culture and Fluorescent Imaging Applications https://www.bioradiations.com/introducing-the-zoe-fluorescent-cell-imager-for-routine-cell-culture-and-fluorescent-imaging-applications/ Fri, 15 May 2015 22:06:25 +0000 http://www.bioradiations.com/?p=6851 Using traditional fluorescence microscopes for everyday cell imaging can be frustrating, with a lot of time spent in the darkroom mastering a highly specialized piece of equipment, all to accomplish relatively simple tasks. The ZOE Fluorescent Cell Imager eliminates the complexities of cell imaging by combining the ease of use of a personal tablet with the power of an inverted microscope. Check it out.

The post Introducing the ZOE™ Fluorescent Cell Imager for Routine Cell Culture and Fluorescent Imaging Applications first appeared on Bio-Radiations.]]> SureBeads™ Magnetic Beads Standard Immunoprecipitation Protocol https://www.bioradiations.com/surebeads-magnetic-beads-standard-immunoprecipitation-protocol/ Mon, 11 May 2015 21:00:43 +0000 http://www.bioradiations.com/?p=6805 Immunoprecipitation (IP) is a commonly used protocol for concentrating and purifying proteins using specific antibodies and magnetic beads. Here is a simple IP protocol using Bio-Rad’s SureBeads Magnetic Beads.

The post SureBeads™ Magnetic Beads Standard Immunoprecipitation Protocol first appeared on Bio-Radiations.]]> What effects are microplastics having on ocean life? https://www.bioradiations.com/what-effects-are-microplastics-having-on-ocean-life/ Fri, 08 May 2015 22:28:20 +0000 http://www.bioradiations.com/?p=6785 Large amounts of plastics are entering our oceans, with especially high levels found near coastal cities and, most famously, in the Great Pacific garbage patch. But what are the effects of all this plastic on the life that makes up ocean ecosystems? A new review looks at just how scientists are answering this question, from measuring these pollutants to understanding their interactions with biological systems.

The post What effects are microplastics having on ocean life? first appeared on Bio-Radiations.]]> Human Gene Knockouts Predict Disease Risk https://www.bioradiations.com/human-gene-knockouts-predict-disease-risk/ Fri, 08 May 2015 22:25:29 +0000 http://www.bioradiations.com/?p=6783 Researchers have used “knockout” mice for decades to study how loss of gene function contributes to diseases. Recently, researchers at the University of Texas Health Science Center (UT Health) at Houston have investigated this gene-disease relationship using naturally occurring “knockout humans.”

The post Human Gene Knockouts Predict Disease Risk first appeared on Bio-Radiations.]]> Find the Latest Research and Information on Parkinson’s Disease In a New Open Access Journal https://www.bioradiations.com/find-the-latest-research-and-information-on-parkinsons-disease-in-a-new-open-access-journal/ Fri, 08 May 2015 22:07:06 +0000 http://www.bioradiations.com/?p=6780 Nature Publishing Group has recently introduced a new, free journal dedicated to Parkinson’s disease (PD). This new open-access journal aims to present both the latest research findings and advances on PD, as well as ideas and options for care and treatment, and information on drugs and clinical trials. The journal will also have brief summaries of research work presented in a manner that is easily understood by anybody seeking information on this disease.

The post Find the Latest Research and Information on Parkinson’s Disease In a New Open Access Journal first appeared on Bio-Radiations.]]> Simple Measurement Could Bring Abandoned Antibiotics Back to the Clinic https://www.bioradiations.com/simple-measurement-could-bring-abandoned-antibiotics-back-to-the-clinic/ Fri, 08 May 2015 21:55:29 +0000 http://www.bioradiations.com/?p=6776 Scientists at Duke University have developed a simple method for fine-tuning antibiotic dosing protocols that could allow use of antibiotics that have been all but abandoned. Computer models and preliminary laboratory work suggest that by determining a pathogen’s recovery time after exposure to an antibiotic, a dosing regimen can be established that allows clearing of what at present are considered resistant strains.

The post Simple Measurement Could Bring Abandoned Antibiotics Back to the Clinic first appeared on Bio-Radiations.]]> Novel Blood Test Predicts Breast Cancer Up to 7 Years Before it Occurs https://www.bioradiations.com/novel-blood-test-predicts-breast-cancer-up-to-7-years-before-it-occurs/ Fri, 08 May 2015 21:44:29 +0000 http://www.bioradiations.com/?p=6772 Mammograms have been used for nearly six decades to screen for breast cancer. However, a new blood test may accurately detect breast cancer years before it develops, according to a recently published article in Metabolomics.

The post Novel Blood Test Predicts Breast Cancer Up to 7 Years Before it Occurs first appeared on Bio-Radiations.]]> Heterochromatin — Key Player in the Aging Process? https://www.bioradiations.com/heterochromatin-key-player-in-the-aging-process/ Fri, 08 May 2015 21:37:58 +0000 http://www.bioradiations.com/?p=6764 In a recently published paper in Science, scientists at the Salk Institute and Chinese Academy of Sciences showed that Werner syndrome, a rare genetic disorder that leads to premature aging, is caused by a deterioration of DNA bundles known as heterochromatin.

The post Heterochromatin — Key Player in the Aging Process? first appeared on Bio-Radiations.]]> Bio-Plex Pro Human Inflammation Assays: Performance Data https://www.bioradiations.com/bio-plex-pro-human-inflammation-assays-performance-data/ Wed, 11 Mar 2015 23:29:16 +0000 http://www.bioradiations.com/?p=5985 Bio-Rad’s Bio-Plex Pro Human Inflammation Assay measures 37 of the most relevant inflammation biomarkers, including TNF superfamily proteins, Treg cytokines, IFN proteins, and matrix metalloproteinases, in a single multiplex panel.Check out the performance characteristics of the assay panel in this report.

The post Bio-Plex Pro Human Inflammation Assays: Performance Data first appeared on Bio-Radiations.]]> Tips for Keeping Your NGC™ Chromatography System in Great Shape https://www.bioradiations.com/tips-for-keeping-your-ngc-chromatography-system-in-great-shape/ Wed, 11 Feb 2015 00:19:06 +0000 http://www.bioradiations.com/?p=5248 Doing these three simple checks will protect your chromatography system from common lab hazards.

The post Tips for Keeping Your NGC™ Chromatography System in Great Shape first appeared on Bio-Radiations.]]> Tips for Using Chromatography Columns with Your NGC™ Chromatography System https://www.bioradiations.com/tips-for-using-chromatography-columns-with-your-ngc-chromatography-system/ Wed, 11 Feb 2015 00:14:47 +0000 http://www.bioradiations.com/?p=5245 Here are the methods you need to ensure optimal, long-lasting column performance.

The post Tips for Using Chromatography Columns with Your NGC™ Chromatography System first appeared on Bio-Radiations.]]> Tips for Sample Preparation with Your NGC™ Chromatography System https://www.bioradiations.com/tips-for-sample-preparation-with-your-ngc-chromatography-system/ Wed, 11 Feb 2015 00:09:47 +0000 http://www.bioradiations.com/?p=5241 Follow these steps to go from crude cell lysate to a sample that’s ready to purify.

The post Tips for Sample Preparation with Your NGC™ Chromatography System first appeared on Bio-Radiations.]]> Tips for Storing Proteins Purified on Your NGC™ Chromatography System https://www.bioradiations.com/tips-for-storing-proteins-purified-on-your-ngc-chromatography-system/ Wed, 11 Feb 2015 00:03:22 +0000 http://www.bioradiations.com/?p=5238 Get the most out of your proteins, including better stability, the best ways to freeze fractions, and more!

The post Tips for Storing Proteins Purified on Your NGC™ Chromatography System first appeared on Bio-Radiations.]]> Tips for Preparing and Using Buffers with Your NGC™ Chromatography System https://www.bioradiations.com/tips-for-preparing-and-using-buffers-with-your-ngc-chromatography-system/ Tue, 10 Feb 2015 19:28:57 +0000 http://www.bioradiations.com/?p=5219 Here are four fast and easy things that will help you maintain peak performance for your medium pressure chromatography system. Beat contamination and save your samples!

The post Tips for Preparing and Using Buffers with Your NGC™ Chromatography System first appeared on Bio-Radiations.]]> Bioradiations Looks Back: 50 Years as Bio-Rad’s Life Science Resource https://www.bioradiations.com/bioradiations-looks-back-50-years-as-bio-rads-life-science-resource/ Tue, 13 Jan 2015 21:15:03 +0000 http://www.bioradiations.com/?p=4441 Bioradiations, Bio-Rad’s technical resource for life science research, sent out its first issue in 1965. How did this publication evolve in the years that followed? Take a look in our archives and find out!

The post Bioradiations Looks Back: 50 Years as Bio-Rad’s Life Science Resource first appeared on Bio-Radiations.]]> PureBlu™ Hoechst 33342 Nuclear Staining Dye for Live Cells – A Fast Approach to Staining Nuclei https://www.bioradiations.com/pureblu-hoechst-33342-nuclear-staining-dye-for-live-cells-a-fast-approach-to-staining-nuclei/ Mon, 12 Jan 2015 20:00:47 +0000 http://www.bioradiations.com/?p=4416 PureBlu Hoechst 33342 Nuclear Staining Dye is the optimal choice for staining the nuclei of live cells for ultraviolet imaging. This short video goes through the steps involved in staining live cells with PureBlu Hoechst 33342 Nuclear Staining Dye.

The post PureBlu™ Hoechst 33342 Nuclear Staining Dye for Live Cells – A Fast Approach to Staining Nuclei first appeared on Bio-Radiations.]]> How to Build a Custom PrimePCR™ Real-Time PCR Plate Using a Suggested or Blank Template https://www.bioradiations.com/how-to-build-a-custom-primepcr-real-time-pcr-plate-using-a-suggested-or-blank-template/ Mon, 12 Jan 2015 20:00:45 +0000 http://www.bioradiations.com/?p=4422 It’s fast and easy to build your own PrimePCR plate. This video walks you through using the Custom Plate Configurator at Bio-Rad.com to create your own 96- or 384-well PCR plate.

The post How to Build a Custom PrimePCR™ Real-Time PCR Plate Using a Suggested or Blank Template first appeared on Bio-Radiations.]]> PrimePCR™ Pathway and Disease Panels – How to Modify a Predesigned Plate https://www.bioradiations.com/primepcr-pathway-and-disease-panels-how-to-modify-a-predesigned-plate/ Mon, 12 Jan 2015 20:00:45 +0000 http://www.bioradiations.com/?p=4420 One easy way to obtain a PrimePCR plate customized for your research is to modify one of Bio-Rad’s predesigned panels. This short video tutorial shows you how to use the online tools at Bio-Rad.com to edit a predesigned plate and create, then order your own customized 96- or 384-well PCR plate.

The post PrimePCR™ Pathway and Disease Panels – How to Modify a Predesigned Plate first appeared on Bio-Radiations.]]> PureBlu™ DAPI Nuclear Staining Dye for Fixed Cells – A Fast Approach to Staining Nuclei https://www.bioradiations.com/pureblu-dapi-nuclear-staining-dye-for-fixed-cells-a-fast-approach-to-staining-nuclei/ Mon, 12 Jan 2015 20:00:43 +0000 http://www.bioradiations.com/?p=4426 PureBlu DAPI Nuclear Staining Dye is the optimal choice for staining the nuclei of fixed cells for ultraviolet imaging. This short video goes through the steps involved in staining live cells with PureBlu DAPI Nuclear Staining Dye.

The post PureBlu™ DAPI Nuclear Staining Dye for Fixed Cells – A Fast Approach to Staining Nuclei first appeared on Bio-Radiations.]]> Cross-Reactivity of Bio-Plex Pro™ Human Cancer Biomarker Assays to Mouse Proteins https://www.bioradiations.com/cross-reactivity-of-bio-plex-pro-human-cancer-biomarker-assays-to-mouse-proteins/ Wed, 10 Dec 2014 21:26:03 +0000 http://www.bioradiations.com/?p=2747 Mice are an optimal model organism for human cancer studies. However, when experiments involve a human xenograft in a mouse host, cross-reactivity presents a major concern in measuring biomarkers. This technical report evaluates 14 Bio-Plex Pro Human Cancer Biomarker Assays for cross reactivity to mouse proteins and discusses the resulting data.

The post Cross-Reactivity of Bio-Plex Pro™ Human Cancer Biomarker Assays to Mouse Proteins first appeared on Bio-Radiations.]]> Investigating Cancer Stem Cells with the S3™ Cell Sorter https://www.bioradiations.com/investigating-cancer-stem-cells-with-the-s3-cell-sorter/ Tue, 09 Dec 2014 23:54:02 +0000 http://www.bioradiations.com/?p=6355 The Cancer Stem Cell model proposes that a special group of dormant cells in the cancer population plays a key role in tumor development and growth, as well as regeneration following treatment. Find out how researchers Stacy Blain and Danielle Joseph are using the S3 Cell Sorter in studying potential cancer stem cells for multiple myeloma.

The post Investigating Cancer Stem Cells with the S3™ Cell Sorter first appeared on Bio-Radiations.]]> Investigating Cancer Stem Cells with the S3™ Cell Sorter https://www.bioradiations.com/investigating-cancer-stem-cells-with-the-s3-cell-sorter-video/ Tue, 09 Dec 2014 20:00:56 +0000 http://www.bioradiations.com/?p=4430 At SUNY Downstate Medical Center in Brooklyn, New York, researchers Stacy Blain and Danielle Joseph are trying to find out how the Cancer Stem Cell model might apply to multiple myeloma. See how they use Bio-Rad’s S3 Cell Sorter to isolate a small population of cells that might play an important role in this disease.

The post Investigating Cancer Stem Cells with the S3™ Cell Sorter first appeared on Bio-Radiations.]]> How to Order PrimePCR™ Assays – Primers, Probes, and Panels https://www.bioradiations.com/how-to-order-primepcr-assays-primers-probes-and-panels/ Tue, 09 Dec 2014 20:00:48 +0000 http://www.bioradiations.com/?p=4424 A wide range of PCR assays is available through Bio-Rad.com for use with real-time or Droplet Digital™ PCR systems. Get a quick refresher on ordering these products with this short video.

The post How to Order PrimePCR™ Assays – Primers, Probes, and Panels first appeared on Bio-Radiations.]]> You Get to Do This? – The Bio-Rad Science Ambassador Program at the 2014 Bay Area Science Festival https://www.bioradiations.com/you-get-to-do-this-the-bio-rad-science-ambassador-program-at-the-2014-bay-area-science-festival/ Tue, 09 Dec 2014 20:00:46 +0000 http://www.bioradiations.com/?p=4434 The Bio-Rad Science Ambassador Program participated in the 2014 Bay Area Science Festival's Discovery Days at AT&T Park event. See one volunteer Science Ambassador talk about conducting a real DNA experiment with students.

The post You Get to Do This? – The Bio-Rad Science Ambassador Program at the 2014 Bay Area Science Festival first appeared on Bio-Radiations.]]> Now I Have My Own DNA – The Bio-Rad Science Ambassador Program at the 2014 Bay Area Science Festival https://www.bioradiations.com/now-i-have-my-own-dna-the-bio-rad-science-ambassador-program-at-the-2014-bay-area-science-festival/ Tue, 09 Dec 2014 20:00:28 +0000 http://www.bioradiations.com/?p=4432 See a range of students talk about the unique experience of extracting their own DNA, and one volunteer Science Ambassador talk about conducting a real DNA experiment with students.

The post Now I Have My Own DNA – The Bio-Rad Science Ambassador Program at the 2014 Bay Area Science Festival first appeared on Bio-Radiations.]]> Discovery Days at World Series HQ, Mini Makers, and More: The Bio-Rad Science Ambassador Program in 2014 and Beyond https://www.bioradiations.com/discovery-days-at-world-series-hq-mini-makers-and-more-the-bio-rad-science-ambassador-program-in-2014-and-beyond/ Tue, 09 Dec 2014 20:00:06 +0000 http://www.bioradiations.com/?p=3239 From running science experiments on the home field of World Series champions the San Francisco Giants, to connecting with makers in “the nerd nest,” to hundreds of new classroom events, 2014 has been an exciting year for the Bio-Rad Science Ambassador Program. Learn about the latest highlights in this corporate social responsibility program and watch a video of participating students and scientists.

The post Discovery Days at World Series HQ, Mini Makers, and More: The Bio-Rad Science Ambassador Program in 2014 and Beyond first appeared on Bio-Radiations.]]> Top Stories of 2014 — Bioradiations Year in Review https://www.bioradiations.com/top-stories-of-2014-bioradiations-year-in-review/ Tue, 09 Dec 2014 19:15:26 +0000 http://www.bioradiations.com/?p=2822 Bioradiations.com, the online technical resource for Bio-Rad customers, has been offering a variety of research tips, customer stories, articles of general interest, and in-depth technical reports throughout the year. We also started a special Bench Partner Video Series, providing quick solutions and ideas for possible problems. Here are the highlights of what we presented in the year 2014.

The post Top Stories of 2014 — Bioradiations Year in Review first appeared on Bio-Radiations.]]> Ebola: New Techniques for Diagnosis, Treatment, and Prevention https://www.bioradiations.com/ebola-new-techniques-for-diagnosis-treatment-and-prevention/ Tue, 09 Dec 2014 18:23:35 +0000 http://www.bioradiations.com/?p=3216 The 2014 West African Ebola outbreak has been an unprecedented public health emergency, with thousands infected across multiple countries and cases spreading internationally. This article examines the science behind this deadly hemorrhagic fever, including its pathogenesis, detection, the challenges it presents in the field, and research into new therapies such as monoclonal antibodies.

The post Ebola: New Techniques for Diagnosis, Treatment, and Prevention first appeared on Bio-Radiations.]]> The Brain’s GPS — Unraveling the Functioning of Our Navigation System https://www.bioradiations.com/the-brains-gps-unraveling-the-functioning-of-our-navigation-system/ Tue, 11 Nov 2014 20:00:44 +0000 http://www.bioradiations.com/?p=2036 This year’s Nobel Prize in Physiology and Medicine was awarded to three neuroscientists for their contribution in elucidating the “GPS of the brain”. Explore how this positioning system was deciphered, what the individual components of this system are, and how they integrate together to help us in our spatial memory and navigation.

The post The Brain’s GPS — Unraveling the Functioning of Our Navigation System first appeared on Bio-Radiations.]]> Biomarker Profiling in Diabetes and Cardiovascular Disease https://www.bioradiations.com/biomarker-profiling-in-diabetes-and-cardiovascular-disease/ Thu, 06 Nov 2014 18:40:05 +0000 http://www.bioradiations.com/?p=4024 In this presentation, Dr. James Mapes details how Bio-Plex Pro™ Human RBM Metabolic and Hormone Assays can be used to assess multiple protein biomarkers associated with diabetes and metabolic conditions linked to cardiovascular diseases. Learn what biomarkers are currently under investigation and why rigorous assay validation is critical.

The post Biomarker Profiling in Diabetes and Cardiovascular Disease first appeared on Bio-Radiations.]]> Using Droplet Digital™ PCR to Monitor Environmental Contaminants in Water https://www.bioradiations.com/using-droplet-digital-pcr-to-monitor-environmental-contaminants-in-water/ Wed, 15 Oct 2014 19:00:10 +0000 http://www.bioradiations.com/?p=4436 South of Los Angeles, near some of America’s most famous beaches, one small agency is working on new methods for coastal water monitoring. Watch this video and see how scientists from SCCWRP, the Southern California Coastal Water Research Project, are using Droplet Digital PCR to watch over swimmer safety and ensure a clean environment.

The post Using Droplet Digital™ PCR to Monitor Environmental Contaminants in Water first appeared on Bio-Radiations.]]> Protecting Coastal Ecosystems with Droplet Digital™ PCR https://www.bioradiations.com/protecting-coastal-ecosystems-with-droplet-digital-pcr/ Tue, 14 Oct 2014 19:00:24 +0000 http://www.bioradiations.com/?p=2025 Who’s watching over swimmer safety at America’s beaches? Go inside one agency tasked with pioneering new methods for water quality monitoring, and see how its scientists are employing Droplet Digital PCR technology to check coastal waters for harmful pathogens.

The post Protecting Coastal Ecosystems with Droplet Digital™ PCR first appeared on Bio-Radiations.]]> Using the ChemiDoc Touch Imaging System to Find Leukemia Targets at UCSF https://www.bioradiations.com/using-the-chemidoc-touch-imaging-system-to-find-leukemia-targets-at-ucsf/ Thu, 09 Oct 2014 00:50:18 +0000 http://www.bioradiations.com/?p=733 Discover how researcher Ernesto Diaz-Flores uses the ChemiDoc Touch Imaging System as he investigates therapeutic targets for a subtype of childhood leukemia. An avid photographer, Diaz-Flores also talks about the ...

The post Using the ChemiDoc Touch Imaging System to Find Leukemia Targets at UCSF first appeared on Bio-Radiations.]]> NGC System Tour and Configurator https://www.bioradiations.com/ngc-system-tour-and-configurator/ Wed, 08 Oct 2014 22:58:50 +0000 http://www.bioradiations.com/?p=719 The NGC Chromatography System introduces a new level of modularity and usability for protein purification. The modular design of the NGC System provides multiple configurations, each addressing different requirements. Use our configurator tool to build your own system to meet your specific workflow and throughput needs.

The post NGC System Tour and Configurator first appeared on Bio-Radiations.]]> Bio-Rad’s S3 Cell Sorter Development: Redesigning Cell Sorting Technology https://www.bioradiations.com/bio-rads-s3-cell-sorter-development-redesigning-cell-sorting-technology/ Wed, 08 Oct 2014 00:54:43 +0000 http://www.bioradiations.com/?p=735 Take a look into the development of Bio-Rad’s S3 Cell Sorter, and see the fresh approach to cell sorting technology that resulted in an innovative new design.

The post Bio-Rad’s S3 Cell Sorter Development: Redesigning Cell Sorting Technology first appeared on Bio-Radiations.]]> Modeling Natural Killer Cell Receptors with the NGC Chromatography System https://www.bioradiations.com/modeling-natural-killer-cell-receptors-with-the-ngc-chromatography-system/ Tue, 07 Oct 2014 00:58:37 +0000 http://www.bioradiations.com/?p=738 See how researcher Petr Novák, a structural biologist in Prague, Czech Republic, has accelerated his effort to characterize complex receptor molecules by integrating the NGC chromatography system into his lab’s ...

The post Modeling Natural Killer Cell Receptors with the NGC Chromatography System first appeared on Bio-Radiations.]]> Bio-Rad Science Ambassador Program Reaches New Age Groups, More Schools https://www.bioradiations.com/bio-rad-science-ambassador-program-reaches-new-age-groups-more-schools/ Wed, 10 Sep 2014 02:55:44 +0000 http://www.bioradiations.com/?p=657 The Science Ambassador Program continues to grow in its mission to connect working scientists with students at the elementary, middle, and high school level. This month, bioradiations catches up with Bio-Rad’s corporate citizenship initiative at two events: the USA STEM festival in Washington, D.C., and a special day of science in Bio-Rad’s home town, Hercules, CA.

The post Bio-Rad Science Ambassador Program Reaches New Age Groups, More Schools first appeared on Bio-Radiations.]]> Trends in Protein Separation and Analysis — the Advance of Stain-Free Technology https://www.bioradiations.com/trends-in-protein-separation-and-analysis-the-advance-of-stain-free-technology/ Tue, 09 Sep 2014 23:45:23 +0000 http://www.bioradiations.com/?p=2011 What is stain-free technology? How does it work in electrophoresis and western blotting? How does it compare with existing staining techniques? What are its advantages and possible concerns? Here is a technical review of this rapidly growing technological advance.

The post Trends in Protein Separation and Analysis — the Advance of Stain-Free Technology first appeared on Bio-Radiations.]]> Telling the Story of Western Blots with the ChemiDoc Touch Imaging System https://www.bioradiations.com/telling-the-story-of-three-western-blots-with-the-chemidoc-touch-imaging-system/ Wed, 13 Aug 2014 01:55:55 +0000 http://www.bioradiations.com/?p=618 For a long time X-ray film has been the standard for imaging western blots, but this method has its limitations. Find out how 3 researchers are using the digital tools offered by the new ChemiDoc Touch Imaging System to beat these limitations and improve their blot images.

The post Telling the Story of Western Blots with the ChemiDoc Touch Imaging System first appeared on Bio-Radiations.]]> ZOE™ Fluorescent Cell Imager https://www.bioradiations.com/zoe-fluorescent-cell-imager/ Sat, 02 Aug 2014 19:14:48 +0000 http://www.bioradiations.com/?p=728 Launch the ZOE Interactive Web App to try the intuitive ZOE user interface, manipulate images, view features and specifications in depth, and more.

The post ZOE™ Fluorescent Cell Imager first appeared on Bio-Radiations.]]> Advanced Topics in Cell Sorting https://www.bioradiations.com/advanced-topics-in-cell-sorting/ Tue, 22 Jul 2014 23:53:09 +0000 http://www.bioradiations.com/?p=3916 In this presentation by Dan Fox of Propel Labs, learn the principles of droplet-based cell sorting, how sorting speed affects results, and how to assess cell sorter performance. Modes and advantages of the S3™ Cell Sorter are also covered.

The post Advanced Topics in Cell Sorting first appeared on Bio-Radiations.]]> Purification Trends: How Smart is Chromatography Automation? https://www.bioradiations.com/purification-trends-how-smart-is-chromatography-automation/ Wed, 09 Jul 2014 04:56:59 +0000 http://www.bioradiations.com/?p=614 What does automated protein purification mean today? What might it look like tomorrow? Bioradiations talks to a variety of users about what they’re doing now to implement automation in their purification processes, and what possibilities they envision that will improve chromatography in the near future.

The post Purification Trends: How Smart is Chromatography Automation? first appeared on Bio-Radiations.]]> Troubleshooting the Bio-Plex® 200 Multiplex Immunoassay System: Device Time-Out https://www.bioradiations.com/troubleshooting-the-bio-plex-200-multiplex-immunoassay-system-device-time-out/ Thu, 12 Jun 2014 19:00:40 +0000 http://www.bioradiations.com/?p=1442 A “device time-out” message on the Bio-Plex® 200 System occurs when the instrument’s needle or probe cannot move correctly. Watch this video for several troubleshooting steps to try when this message occurs.

The post Troubleshooting the Bio-Plex® 200 Multiplex Immunoassay System: Device Time-Out first appeared on Bio-Radiations.]]> Multiplex Analysis of Cell Death Markers Using Bio-Plex Pro™ RBM Apoptosis Assays https://www.bioradiations.com/multiplex-analysis-of-cell-death-markers-using-bio-plex-pro-rbm-apoptosis-assays/ Wed, 11 Jun 2014 00:06:53 +0000 http://www.bioradiations.com/?p=1906 Bio-Rad’s new Bio-Plex Pro RBM Apoptosis Multiplex Assays enable complex, quantitative analyses of biomarkers in the intrinsic apoptosis pathway. Learn more about these biomarkers and view sample data and performance characteristics of these assays in this detailed technical report.

The post Multiplex Analysis of Cell Death Markers Using Bio-Plex Pro™ RBM Apoptosis Assays first appeared on Bio-Radiations.]]> Genomic Variation Analysis with Droplet Digital PCR: Success Stories https://www.bioradiations.com/genomic-variation-analysis-with-droplet-digital-pcr-success-stories/ Tue, 10 Jun 2014 22:38:18 +0000 http://www.bioradiations.com/?p=1024 Researchers are having success applying Droplet Digital PCR (ddPCR™) technology across many different fields. Here are two such success stories, one involving the application of ddPCR in detecting mutations in iPS cells, the other in predicting allele-specific variation in human brain development.

The post Genomic Variation Analysis with Droplet Digital PCR: Success Stories first appeared on Bio-Radiations.]]> Brighter Precision Plus Protein™ Dual Color Standard — The Brightest Protein Marker Available https://www.bioradiations.com/brighter-precision-plus-protein-dual-color-standard-the-brightest-protein-marker-available/ Tue, 10 Jun 2014 20:31:25 +0000 http://www.bioradiations.com/?p=3519 Take a quick look at Bio-Rad’s brighter Precision Plus Protein™ Dual Color Standard with this article comparing this standard to other available protein markers. Plus, find out why the ability to visualize a protein standard after reprobing matters to one researcher!

The post Brighter Precision Plus Protein™ Dual Color Standard — The Brightest Protein Marker Available first appeared on Bio-Radiations.]]> Better, Faster, and Less Expensive Gene Expression Analysis Using Multiplex One-Step RT-qPCR: A Case Study of Colorectal Cancer https://www.bioradiations.com/better-faster-and-less-expensive-gene-expression-analysis-using-multiplex-one-step-rt-qpcr-a-case-study-of-colorectal-cancer/ Tue, 10 Jun 2014 19:00:50 +0000 http://www.bioradiations.com/?p=1731 Conducting a triplex one-step RT-qPCR assay promises a more efficient and economical analysis of gene expression compared to running three individual assays. This technical report describes a colorectal cancer case study where a triplex multiplex assay was validated.

The post Better, Faster, and Less Expensive Gene Expression Analysis Using Multiplex One-Step RT-qPCR: A Case Study of Colorectal Cancer first appeared on Bio-Radiations.]]> Troubleshooting the Bio-Plex® 200 Multiplex Immunoassay System: HTF Alarm https://www.bioradiations.com/troubleshooting-the-bio-plex-200-multiplex-immunoassay-system-htf-alarm/ Tue, 10 Jun 2014 19:00:30 +0000 http://www.bioradiations.com/?p=1433 The high-throughput fluidics (HTF) alarm on the Bio-Plex® 200 System indicates a problem with the instrument’s sheath fluid delivery system. See the actions needed to quickly resolve potential issues with this system and get back to your research!

The post Troubleshooting the Bio-Plex® 200 Multiplex Immunoassay System: HTF Alarm first appeared on Bio-Radiations.]]> Troubleshooting the Bio-Plex® 200 Multiplex Immunoassay System: Device Failed to Pressurize https://www.bioradiations.com/troubleshooting-the-bio-plexr-200-multiplex-immunoassay-system-device-failed-to-pressurize/ Tue, 10 Jun 2014 17:55:20 +0000 http://www.bioradiations.com/?p=1437 During calibration of your Bio-Plex® 200 System, you may receive a “failed to pressurize message.” In this short video, get some easy fixes for the most common issues prompting this message.

The post Troubleshooting the Bio-Plex® 200 Multiplex Immunoassay System: Device Failed to Pressurize first appeared on Bio-Radiations.]]> How to Remove Stripes from a Gel Image — ChemiDoc™ Imager Filter Alignment https://www.bioradiations.com/how-to-remove-stripes-from-a-gel-image-chemidoc-imager-filter-alignment/ Sat, 31 May 2014 23:10:56 +0000 http://www.bioradiations.com/?p=1394 Usually when stripes appear on a gel image produced with a ChemiDoc XRS+ or ChemiDoc MP Imaging System, a misaligned filter is the cause. Learn the simple steps to correct this issue with this video from Bench Partners.

The post How to Remove Stripes from a Gel Image — ChemiDoc™ Imager Filter Alignment first appeared on Bio-Radiations.]]> Ligand Immobilization in Protein Interaction Studies — An Unattended Amine Coupling Protocol with Automatic Coinjection Activation https://www.bioradiations.com/ligand-immobilization-in-protein-interaction-studies-an-unattended-amine-coupling-protocol-with-automatic-co-injection-activation/ Fri, 16 May 2014 19:00:40 +0000 http://www.bioradiations.com/?p=1447 Amine coupling is the most commonly adopted technique to bind proteins to biosensor chips. Because of the possibility of the amine coupling reagents reacting with each other, these have to be premixed just before the injection and injected independently. Here are some tips to automatically coinject these reagents.

The post Ligand Immobilization in Protein Interaction Studies — An Unattended Amine Coupling Protocol with Automatic Coinjection Activation first appeared on Bio-Radiations.]]> Bio-Rad’s Universal Real-Time PCR App – Everything You Need to Know about Real-Time PCR at Your Fingertips https://www.bioradiations.com/bio-rads-universal-real-time-pcr-app-everything-you-need-to-know-about-real-time-pcr-at-your-fingertips/ Thu, 15 May 2014 23:10:06 +0000 http://www.bioradiations.com/?p=725 Bio-Rad’s Universal Real-Time PCR Kits and Reagents work on all real-time PCR systems. Now you can easily compare performance data and get supporting documents for these kits and reagents using Bio-Rad’s new tablet and web app. Learn more and download the app here.

The post Bio-Rad’s Universal Real-Time PCR App – Everything You Need to Know about Real-Time PCR at Your Fingertips first appeared on Bio-Radiations.]]> Developing Bio-Rad’s S3™ Cell Sorter: The Great Cell Sorting Redesign https://www.bioradiations.com/developing-bio-rads-s3-cell-sorter-the-great-cell-sorting-redesign/ Thu, 15 May 2014 04:53:34 +0000 http://www.bioradiations.com/?p=993 Cell sorting technology is not new. However, sometimes taking a new look at a mature technology brings unexpected results. See how we reimagined drop delay calculation, rethought software and automation, and more during the design and development of Bio-Rad’s S3 Cell Sorter.

The post Developing Bio-Rad’s S3™ Cell Sorter: The Great Cell Sorting Redesign first appeared on Bio-Radiations.]]> Protein Purification Workflow Development Using Bio-Rad’s NGC™ Chromatography System https://www.bioradiations.com/protein-purification-workflow-development-using-bio-rads-ngc-chromatography-system/ Tue, 13 May 2014 18:03:19 +0000 http://www.bioradiations.com/?p=1807 While using affinity tagging is the preferred method to achieve highly pure protein through recombinant expression, this approach isn’t feasible for all proteins. This tech report describes workflow optimizations using the NGC Chromatography System that allow users to achieve high purities with untagged proteins.

The post Protein Purification Workflow Development Using Bio-Rad’s NGC™ Chromatography System first appeared on Bio-Radiations.]]> Single-Cell Western Blot and Stain-Free Total Protein Loading Control https://www.bioradiations.com/single-cell-western-blot-and-stain-free-total-protein-loading-control/ Mon, 28 Apr 2014 20:48:54 +0000 http://www.bioradiations.com/?p=3869 Dr. Robyn Murphy of Latrobe University introduces a protocol using Bio-Rad’s stain-free gel technology to conduct western blotting on sample sizes as small as single muscle cells. Dr. Murphy shows ...

The post Single-Cell Western Blot and Stain-Free Total Protein Loading Control first appeared on Bio-Radiations.]]> Can We Trust Western Blots? https://www.bioradiations.com/can-we-trust-western-blots/ Mon, 28 Apr 2014 18:55:38 +0000 http://www.bioradiations.com/?p=3787 Prof. Aldrin Gomes of UC Davis shares western blot problems he has seen as a journal editor and outlines standardized procedures and controls needed to make western blotting data reliable. The presentation also includes tips to save time and labor during the blotting procedure.

The post Can We Trust Western Blots? first appeared on Bio-Radiations.]]> Unlocking the Structures of Natural Killer Cell Receptors with the NGC™ Chromatography System https://www.bioradiations.com/unlocking-the-structures-of-natural-killer-cell-receptors-with-the-ngc-chromatography-system/ Tue, 08 Apr 2014 20:54:47 +0000 http://www.bioradiations.com/?p=1073 Structural biologist Petr Novák employs the latest techniques to model natural killer cell receptor molecules, research that may one day result in powerful therapies to treat cancer and infections. Go inside the fascinating world of structural characterization and find out how Novák has integrated the NGC™ chromatography system into his effort to visualize these molecules in vivid detail.

The post Unlocking the Structures of Natural Killer Cell Receptors with the NGC™ Chromatography System first appeared on Bio-Radiations.]]> Novel Liposome-Capture Surface Chemistries to Analyze Drug-Lipid Interaction Using the ProteOn™ XPR36 System https://www.bioradiations.com/novel-liposome-capture-surface-chemistries-to-analyze-drug-lipid-interaction-using-the-proteon-xpr36-system/ Tue, 08 Apr 2014 19:00:35 +0000 http://www.bioradiations.com/?p=1948 Surface plasmon resonance (SPR) biosensors offer a label-free technique for profiling biomolecular interactions, including those between drugs and liposomes. Here we describe the novel surface chemistries that make capturing liposomes possible, and explore the application of the liposome capturing kit in analyzing liposome-small molecule interactions.

The post Novel Liposome-Capture Surface Chemistries to Analyze Drug-Lipid Interaction Using the ProteOn™ XPR36 System first appeared on Bio-Radiations.]]> DIY Cell Sorting with Bio-Rad’s S3™ Cell Sorter https://www.bioradiations.com/diy-cell-sorting-with-bio-rads-s3-cell-sorter/ Tue, 08 Apr 2014 17:13:56 +0000 http://www.bioradiations.com/?p=3197 Until recently, cell sorting has been a complex endeavor requiring specialized facilities and trained technicians. This infographic reveals how Bio-Rad’s S3 cell sorter makes this technique available to individual, non-expert researchers in 3 easy steps.

The post DIY Cell Sorting with Bio-Rad’s S3™ Cell Sorter first appeared on Bio-Radiations.]]> TC20™ and TC10™ Cell Counters – Cleaning the Print Heads for Clear Printed Results https://www.bioradiations.com/bio-rad-tc20-and-tc10-cell-counters-cleaning-the-print-heads-for-clear-printed-results/ Mon, 07 Apr 2014 20:11:39 +0000 http://www.bioradiations.com/?p=1347 Most problems with misaligned printouts on the printer supplied with the TC20 and TC10 cell counters can be solved by cleaning the print heads. In this short video, find out the fast, easy, and effective way to clean these print heads so you can get back to your research!

The post TC20™ and TC10™ Cell Counters – Cleaning the Print Heads for Clear Printed Results first appeared on Bio-Radiations.]]> Bio-Rad Supply Center — How-To Tips for Setting Up https://www.bioradiations.com/bio-rad-supply-center-how-to-tips-for-setting-up/ Tue, 11 Mar 2014 22:09:50 +0000 http://www.bioradiations.com/?p=5104 Learn the best practices and tips for setting up your Bio-Rad Supply Center.

The post Bio-Rad Supply Center — How-To Tips for Setting Up first appeared on Bio-Radiations.]]> The Ever-Expanding Universe of Droplet Digital™ PCR Applications https://www.bioradiations.com/the-ever-expanding-universe-of-droplet-digital-pcr-applications/ Tue, 11 Mar 2014 21:24:19 +0000 http://www.bioradiations.com/?p=1597 Bio-Rad’s groundbreaking Droplet Digital™ PCR technology can be applied in a variety of applications to glean precious information that cannot be obtained by any other method. Find out more about how this technology has been used in applications such as quantification of cancer biomarkers, validation of next generation sequencing, distinguishing genomic variation, and more.

The post The Ever-Expanding Universe of Droplet Digital™ PCR Applications first appeared on Bio-Radiations.]]> Bio-Rad Supply Center — How-To Tips for Taking Inventory https://www.bioradiations.com/bio-rad-supply-center-how-to-tips-for-setting-up-and-taking-inventory/ Tue, 11 Mar 2014 20:46:07 +0000 http://www.bioradiations.com/?p=1358 In this new video series, Bio-Rad offers answers for some your most frequently asked questions. This installment covers setting up and taking inventory of your Bio-Rad Supply Center.

The post Bio-Rad Supply Center — How-To Tips for Taking Inventory first appeared on Bio-Radiations.]]> Transforming Cancer Research: Droplet Digital PCR Plays Integral Role at Fred Hutchinson Cancer Research Center https://www.bioradiations.com/transforming-cancer-research-droplet-digital-pcr-plays-integral-role-at-fred-hutchinson-cancer-research-center/ Tue, 11 Mar 2014 20:11:22 +0000 http://www.bioradiations.com/?p=1008 Droplet Digital PCR has major implications for cancer studies, where the ability to accurately measure biomarkers may result in important insights into cancer genesis and progression. Find out about new findings on micro RNA and Tumor Infilitrating Lymphocytes made possible by ddPCR technology.

The post Transforming Cancer Research: Droplet Digital PCR Plays Integral Role at Fred Hutchinson Cancer Research Center first appeared on Bio-Radiations.]]> A New Breed of Cell Sorters: Bio-Rad’s S3™ Cell Sorter Appeals to the Growing Cell Biology Community https://www.bioradiations.com/a-new-breed-of-cell-sorters-bio-rads-s3-cell-sorter-appeals-to-the-growing-cell-biology-community/ Tue, 11 Mar 2014 19:00:00 +0000 http://www.bioradiations.com/?p=1769 The S3 cell sorter is Bio-Rad’s new addition to the world of flow cytometry. Take a look at how this instrument breaks the mold for cell sorters, brings relief to investigators and core lab managers alike, and opens up exciting new research possibilities.

The post A New Breed of Cell Sorters: Bio-Rad’s S3™ Cell Sorter Appeals to the Growing Cell Biology Community first appeared on Bio-Radiations.]]> Bio-Plex® Assay Builder https://www.bioradiations.com/bio-plex-assay-builder/ Sun, 02 Mar 2014 23:19:14 +0000 http://www.bioradiations.com/?p=5760 Design your customized Express or x-Plex assay, process your catalog number, and obtain pricing information, launch the Assay Builder

The post Bio-Plex® Assay Builder first appeared on Bio-Radiations.]]> Applications of Bio-Plex Pro™ Cell Signaling Akt and MAPK Assay Panels in Signal Transduction Pathways https://www.bioradiations.com/applications-of-bio-plex-pro-cell-signaling-akt-and-mapk-assay-panels-in-signal-transduction-pathways/ Tue, 11 Feb 2014 20:00:39 +0000 http://www.bioradiations.com/?p=1889 The Akt and MAPK signal transduction pathways are key areas of study for their roles in cell growth, function, and death, as well as for their possible roles in cancer development and diabetes. Bio-Rad’s new Bio-Plex Pro™ Akt and MAPK cell signaling assays enable researchers to study the activation of these key pathways as a response to various stimuli. This technical report validates the sensitivity and specificity of these assays after cells were exposed to insulin, EGF (epidermal growth factor), and UV radiation.

The post Applications of Bio-Plex Pro™ Cell Signaling Akt and MAPK Assay Panels in Signal Transduction Pathways first appeared on Bio-Radiations.]]> Profiling Dynamic Cancer Drug Responses with BioMarker Strategies, LLC and the Bio-Plex® Multiplex Immunoassay System https://www.bioradiations.com/profiling-dynamic-cancer-drug-responses-with-biomarker-strategies-llc-and-the-bio-plex-multiplex-immunoassay-system/ Tue, 11 Feb 2014 19:45:15 +0000 http://www.bioradiations.com/?p=1199 In an age of personalized medicine, getting the most out of highly targeted cancer therapies takes finding the best molecular targets for each patient. BioMarker Strategies LLC, a small startup, has been developing an innovative method to do this by modeling oncogenic pathway responses, but they needed to accurately measure the levels of phosphoproteins resulting from the activation of these genes. Learn how they are using the Bio-Plex multiplex immunoassay system to accurately quantify the proteins and generate functional signaling profiles for these oncogenes.

The post Profiling Dynamic Cancer Drug Responses with BioMarker Strategies, LLC and the Bio-Plex® Multiplex Immunoassay System first appeared on Bio-Radiations.]]> Droplet Digital™ PCR for Gene Expression and MicroRNA Analysis https://www.bioradiations.com/droplet-digital-pcr-for-gene-expression-and-microrna-analysis/ Tue, 04 Feb 2014 18:11:43 +0000 http://www.bioradiations.com/?p=3988 Join Dr. Yann Jouvenot for this overview of Bio-Rad's Droplet Digital PCR (ddPCR™) System. Learn about the principles, unique characteristics, and applications of the technology and find out how it can be used to complement existing techniques in gene expression studies and microRNA analysis.

The post Droplet Digital™ PCR for Gene Expression and MicroRNA Analysis first appeared on Bio-Radiations.]]> Life Since the Double Helix: 60 Years of Evolution in Biotechnology https://www.bioradiations.com/life-since-the-double-helix-60-years-of-evolution-in-biotechnology/ Tue, 14 Jan 2014 22:26:52 +0000 http://www.bioradiations.com/?p=1595 The advances in biological science and its applications in medicine, industry, agriculture, and other fields over the past 60 years have changed the world dramatically. This article highlights the major milestones in biology and biotechnology since the discovery of the DNA double helix.

The post Life Since the Double Helix: 60 Years of Evolution in Biotechnology first appeared on Bio-Radiations.]]> Casting Polyacrylamide Gels with TGX™ and TGX Stain-Free™ FastCast™ Acrylamide Kits https://www.bioradiations.com/casting-polyacrylamide-gels-with-tgx-fastcast-kits/ Tue, 14 Jan 2014 20:19:42 +0000 http://www.bioradiations.com/?p=2166 Want a streamlined, easy workflow for hand casting polyacrylamide gels? This tutorial video shows how to use Bio-Rad’s new FastCast™ acrylamide kits to hand cast gels, saving time while providing the benefits of the latest precast gel chemistries.

The post Casting Polyacrylamide Gels with TGX™ and TGX Stain-Free™ FastCast™ Acrylamide Kits first appeared on Bio-Radiations.]]> Microbes in Space! High School Students Design Experiments for the International Space Station with Bio-Rad’s pGLO™ Bacterial Transformation Kits https://www.bioradiations.com/microbes-in-space-high-school-students-design-experiments-for-the-international-space-station-with-bio-rads-pglo-bacterial-transformation-kits/ Tue, 10 Dec 2013 22:57:32 +0000 http://www.bioradiations.com/?p=1496 Retired satellite engineer Dan Saldana is helping Valley Christian High School in San Jose, CA build a program to send student experiments to the International Space Station. To study microbial growth in microgravity, Saldana and his students turned to Bio-Rad’s pGLO bacterial transformation kits. Learn about the high-flying travels of these glowing bacteria and the science learning that resulted.

The post Microbes in Space! High School Students Design Experiments for the International Space Station with Bio-Rad’s pGLO™ Bacterial Transformation Kits first appeared on Bio-Radiations.]]> Top Stories of 2013 — Bioradiations Year in Review https://www.bioradiations.com/top-stories-of-2013-bioradiations-year-in-review/ Tue, 10 Dec 2013 18:07:32 +0000 http://www.bioradiations.com/?p=1777 From the origins of digital PCR in leukemia research to stopping HIV with novel polymers to better methods for fragment-based drug discovery, BioRadiations has covered some of the most exciting life science research stories of 2013. Now take a look back at these stories and more as we review the best pieces of the year.

The post Top Stories of 2013 — Bioradiations Year in Review first appeared on Bio-Radiations.]]> Droplet Digital™ PCR for Gene Copy Number Variation Analysis https://www.bioradiations.com/droplet-digital-pcr-for-gene-copy-number-variation-analysis/ Thu, 05 Dec 2013 18:19:45 +0000 http://www.bioradiations.com/?p=3992 In this webinar presented by Dr. Jennifer Berman, learn how Droplet Digital PCR (ddPCR™) technology uses a simple, cost-effective workflow to accurately and reproducibly assess copy number variations (CNVs) even in high-throughput settings.

The post Droplet Digital™ PCR for Gene Copy Number Variation Analysis first appeared on Bio-Radiations.]]> Building New Anti-HIV Molecules with Bio-Rad’s ProteOn™ XPR36 System https://www.bioradiations.com/building-new-anti-hiv-molecules-with-bio-rads-proteon-xpr36-system/ Wed, 13 Nov 2013 00:47:47 +0000 http://www.bioradiations.com/?p=1733 Can we construct molecules that will interfere with disease pathways? Dan Mitchell of the University of Warwick and his colleagues wanted to apply new synthesis techniques to interrupting HIV infection. Find out what they learned about the potential of these novel molecules using Bio-Rad’s ProteOn™ XPR36 System.

The post Building New Anti-HIV Molecules with Bio-Rad’s ProteOn™ XPR36 System first appeared on Bio-Radiations.]]> The Chromatography Chronicles Part 5: Chromatography You Can Count On https://www.bioradiations.com/the-chromatography-chronicles-part-5-chromatography-you-can-count-on/ Wed, 13 Nov 2013 00:08:08 +0000 http://www.bioradiations.com/?p=1140 In biochromatography, every purification or separation procedure that fails because of instrument malfunction wastes precious lab resources. That’s why Bio-Rad, in designing the NGC chromatography system, found new ways to engineer reliability into the chromatography experience. Here’s how they did it.

The post The Chromatography Chronicles Part 5: Chromatography You Can Count On first appeared on Bio-Radiations.]]> Bio-Rad’s FastCast™ Acrylamide Kits — It’s Time to Expect More from Your Handcast Gels https://www.bioradiations.com/bio-rads-fastcast-acrylamide-kits-its-time-to-expect-more-from-your-handcast-gels/ Tue, 12 Nov 2013 17:42:49 +0000 http://www.bioradiations.com/?p=3572 Hand casting polyacrylamide gels is not an easy task. It is laborious, needs lot of pre-planning and is cumbersome. Bio-Rad’s new TGX and TGX Stain-Free FastCast gel solutions not only address all these issues, but provide added benefits, such as increased speed, stain-free visualization, and quality control. Learn more about these new handcasting kits.

The post Bio-Rad’s FastCast™ Acrylamide Kits — It’s Time to Expect More from Your Handcast Gels first appeared on Bio-Radiations.]]> Stand Up Against Downtime https://www.bioradiations.com/stand-up-against-downtime/ Mon, 11 Nov 2013 20:00:17 +0000 http://www.bioradiations.com/?p=2168 Facing adversity with humor, some chromatographers are drawing on the healing power of laughter to deal with the frustrations of chromatography systems that just won’t work.

The post Stand Up Against Downtime first appeared on Bio-Radiations.]]> Using Droplet Digital™ PCR for Cancer and Liquid Biopsy Studies https://www.bioradiations.com/using-droplet-digital-pcr-for-cancer-and-liquid-biopsy-studies/ Thu, 07 Nov 2013 18:23:01 +0000 http://www.bioradiations.com/?p=3998 This presentation by Dr. Dawne Shelton describes how Droplet Digital PCR (ddPCR™) can be used to measure circulating nucleic acids (cfDNA) and circulating tumor cells (CTCs) in blood and to detect rare tumorigenic mutations down to 0.01% or less in a high background of normal DNA. Techniques and methods discussed in this webinar will enable you to begin your own investigations.

The post Using Droplet Digital™ PCR for Cancer and Liquid Biopsy Studies first appeared on Bio-Radiations.]]> The Power of Partitioning: The World of Droplet Digital™ PCR Applications https://www.bioradiations.com/the-power-of-partitioning-the-world-of-droplet-digital-pcr-applications/ Tue, 15 Oct 2013 17:23:39 +0000 http://www.bioradiations.com/?p=4002 Droplet Digital PCR (ddPCR™) has revolutionized infectious disease research, cancer biomarker and genomic variation analysis, and other fields. This webinar by Dr. George Karlin-Neumann provides an overview of the ddPCR concept and workflow and discusses recent journal publications showcasing the technology’s diverse applications.

The post The Power of Partitioning: The World of Droplet Digital™ PCR Applications first appeared on Bio-Radiations.]]> The Aha! Moment – Bio Rad Science Ambassadors at the 2013 Day of Innovation https://www.bioradiations.com/the-aha-moment-bio-rad-science-ambassadors-at-the-2013-day-of-innovation/ Tue, 08 Oct 2013 19:00:43 +0000 http://www.bioradiations.com/?p=2171 Enthusiastic students describe the fun they had with the Science Ambassador DNA experiment.

The post The Aha! Moment – Bio Rad Science Ambassadors at the 2013 Day of Innovation first appeared on Bio-Radiations.]]> Learning Curve Blues https://www.bioradiations.com/learning-curve-blues/ Tue, 08 Oct 2013 19:00:18 +0000 http://www.bioradiations.com/?p=2181 This researcher sings a tune many will recognize from their own frustrations with overly complex chromatography instruments. The NGC™ chromatography system offers a simple, user-friendly interface so even students with little experience will beat the “learning curve blues.”

The post Learning Curve Blues first appeared on Bio-Radiations.]]> Efficient SPR-Based Fragment Screening and Small Molecule Affinity Analysis Using Bio-Rad’s ProteOn™ XPR36 System https://www.bioradiations.com/efficient-spr-based-fragment-screening-and-small-molecule-affinity-analysis-using-bio-rads-proteon-xpr36-system/ Tue, 08 Oct 2013 19:00:00 +0000 http://www.bioradiations.com/?p=1941 Fragment-based screening has emerged as an important tool for identifying lead compounds in drug discovery, though the molecules involved present challenges related to their low affinity. Label-free surface plasmon resonance (SPR) analysis provides an efficient and reproducible solution to these challenges. Here we describe ways to determine small molecule affinity that combines high sensitivity and high throughput with low sample consumption.

The post Efficient SPR-Based Fragment Screening and Small Molecule Affinity Analysis Using Bio-Rad’s ProteOn™ XPR36 System first appeared on Bio-Radiations.]]> The Chromatography Chronicles Part 4: Chromatography’s Intuitive Side https://www.bioradiations.com/the-chromatography-chronicles-part-4-chromatographys-intuitive-side/ Wed, 02 Oct 2013 22:24:10 +0000 http://www.bioradiations.com/?p=1132 Chromatography instruments have a bad reputation in terms of their complexity and difficulty of use. In response, Bio-Rad’s designers and engineers challenged themselves to make the new NGC™ chromatography system as simple and intuitive-to-use as possible. Learn about the new easier-to-use instrument interface they created as a result.

The post The Chromatography Chronicles Part 4: Chromatography’s Intuitive Side first appeared on Bio-Radiations.]]> Labs for Inspiration: Making a Difference with Bio-Rad’s Science Ambassador Program https://www.bioradiations.com/labs-for-inspiration-making-a-difference-with-bio-rads-science-ambassador-program/ Wed, 02 Oct 2013 18:42:30 +0000 http://www.bioradiations.com/?p=1665 Jefferson County, Tennessee, faced tight school budgets and a mandate to “teach to the test” that left little room for hands-on science learning. Learn how biology professor Lisa Eccles worked to reverse this trend by getting involved with the Science Ambassador program, Bio-Rad’s fast-growing initiative to spark excitement about life science in primary school classrooms.

The post Labs for Inspiration: Making a Difference with Bio-Rad’s Science Ambassador Program first appeared on Bio-Radiations.]]> Breaking Leukemia’s Limits of Detection with Droplet Digital™ PCR https://www.bioradiations.com/breaking-leukemias-limits-of-detection-with-droplet-digital-pcr-video/ Mon, 16 Sep 2013 21:13:12 +0000 http://www.bioradiations.com/?p=2187 Prof. Alec Morley, a pioneer in digital PCR, describes his use of Droplet Digital PCR (ddPCR™) technology to advance the diagnosis and treatment of leukemia. Morley and his colleagues at Flinders University and Medical Center in Adelaide, South Australia previously developed a digital PCR method for the early detection of residual acute lymphoblastic leukemia (ALL). Learn how Morley is now using Bio-Rad's ddPCR technology to develop a diagnostic test for monitoring chronic myeloid leukemia.

The post Breaking Leukemia’s Limits of Detection with Droplet Digital™ PCR first appeared on Bio-Radiations.]]> Breaking Leukemia’s Limits of Detection with Droplet Digital PCR https://www.bioradiations.com/breaking-leukemias-limits-of-detection-with-droplet-digital-pcr/ Wed, 11 Sep 2013 05:11:54 +0000 http://www.bioradiations.com/?p=524 In the early 1990s, researcher Alec Morley and his colleagues pioneered digital PCR techniques to measure acute lymphoblastic leukemia. Today, Morley is trying to develop a more accurate way to quantify chronic myeloid leukemia. Find out why he chose Bio-Rad’s Droplet Digital PCR technology for this effort.

The post Breaking Leukemia’s Limits of Detection with Droplet Digital PCR first appeared on Bio-Radiations.]]> Bench Buzz – Protein Purification Product Testers https://www.bioradiations.com/bench-buzz-protein-purification-product-testers/ Tue, 10 Sep 2013 19:00:32 +0000 http://www.bioradiations.com/?p=2205 Meet Janna and Tomas. After hearing competing claims about the scalability of next-generation chromatography systems, this dynamic duo took it upon themselves to discover the truth. Now let's face it, when you think of protein purification you don't usually think of good times. But somehow Janna and Tomas manage to keep the mood light even as they put the systems to the test by simulating changing protein purification needs. Their conclusion? Vote Yes for Better Chromatography. Vote Yes for Bio-Rad.

The post Bench Buzz – Protein Purification Product Testers first appeared on Bio-Radiations.]]> The Chromatography Chronicles Part 3: Adding, Swapping, and Scaling — Better Chromatography through Modular Design https://www.bioradiations.com/the-chromatography-chronicles-part-3-adding-swapping-and-scaling-better-chromatography-through-modular-design/ Tue, 10 Sep 2013 18:11:57 +0000 http://www.bioradiations.com/?p=1119 What does it take to configure your own chromatography system? Part three of The Chromatography Chronicles examines how the NGC™ chromatography system adapts and grows to meet changing lab needs, and the modular design that makes this possible.

The post The Chromatography Chronicles Part 3: Adding, Swapping, and Scaling — Better Chromatography through Modular Design first appeared on Bio-Radiations.]]> 2-D Electrophoresis Tips & Tricks — Part I https://www.bioradiations.com/2-d-electrophoresis-tips-tricks-part-i/ Mon, 02 Sep 2013 01:15:34 +0000 http://www.bioradiations.com/?p=3923 In the first half of this two-part presentation, Dr. Anton Posch, an expert in 1- and 2-D electrophoresis, provides tips and tricks for the 2DE workflow, covering sample preparation, protein extraction and solubilization, reduction and alkylation of protein side chains, and IEF. The webinar highlights critical success factors and is aimed at both novices and experts wanting to sharpen their skills and keep up on new advances in the field.

The post 2-D Electrophoresis Tips & Tricks — Part I first appeared on Bio-Radiations.]]> 2-D Electrophoresis Tips & Tricks — Part II https://www.bioradiations.com/2-d-electrophoresis-tips-tricks-part-ii/ Sun, 01 Sep 2013 17:10:54 +0000 http://www.bioradiations.com/?p=3985 In the second part of this presentation, Dr. Posch details IEF separation, use of immobilized pH gradient (IPG) strips, running second-dimension PAGE, spot detection, gel matching, and data analysis. The Q&A elaborates on sample preparation, storage, and running conditions.

The post 2-D Electrophoresis Tips & Tricks — Part II first appeared on Bio-Radiations.]]> Buying a New Preparative Protein Purification System – You Have a New Choice https://www.bioradiations.com/buying-a-new-preparative-protein-purification-system-you-have-a-new-choice/ Tue, 13 Aug 2013 19:00:44 +0000 http://www.bioradiations.com/?p=2222 A day in the life of Maria, a post-graduate scientist. Both Maria and her PI are annoyed with their unreliable, out-dated protein purification system, and her PI asks Maria to buy them a new one. She struggles to find a system that meets all their needs until she remembers that Bio-Rad has a new next-generation chromatography system that will work for everyone!

The post Buying a New Preparative Protein Purification System – You Have a New Choice first appeared on Bio-Radiations.]]> Guide to SPR Data Processing on the ProteOn™ XPR36 System https://www.bioradiations.com/guide-to-data-processing-on-the-proteon-xpr36-protein-interaction-array-system/ Tue, 13 Aug 2013 19:00:00 +0000 http://www.bioradiations.com/?p=1551 Good data processing practice ensures that protein interaction experiments using a surface plasmon resonance (SPR) system will yield the clearest possible results. Here we present tips for understanding and processing SPR sensorgrams when using Bio-Rad’s ProteOn™ XPR36 protein interaction array system.

The post Guide to SPR Data Processing on the ProteOn™ XPR36 System first appeared on Bio-Radiations.]]> The Chromatography Chronicles Part 2: The Chromatographers Who Came in from the Cold https://www.bioradiations.com/the-chromatography-chronicles-part-2-the-chromatographers-who-came-in-from-the-cold/ Tue, 13 Aug 2013 18:00:03 +0000 http://www.bioradiations.com/?p=1111 Is spending hours in the coldroom just an inevitable part of doing chromatography? The engineers and designers at Bio-Rad believed that, with a little innovation, the answer could be “no.” Part two of The Chromatography Chronicles reveals how they freed the NGC™ medium-pressure chromatography system — and its users — from the coldroom’s chilly confines.

The post The Chromatography Chronicles Part 2: The Chromatographers Who Came in from the Cold first appeared on Bio-Radiations.]]> Biologics Analysis Workflow™ – Delivering Increased Throughput, Sensitivity, Reproducibility, and Automation for cGMP Laboratories https://www.bioradiations.com/biologics-analysis-workflow-delivering-increased-throughput-sensitivity-reproducibility-and-automation-for-cgmp-laboratories/ Mon, 12 Aug 2013 17:04:33 +0000 http://www.bioradiations.com/?p=3584 Evaluating potential protein therapies for purity and/or identity represents a vital and highly regulated step in biological drug development. Bio-Rad’s new Biologics Analysis Workflow™ offers a high-throughput, sensitive, and reproducible solution that is cGMP-compliant.

The post Biologics Analysis Workflow™ – Delivering Increased Throughput, Sensitivity, Reproducibility, and Automation for cGMP Laboratories first appeared on Bio-Radiations.]]> Bio-Rad Expert Care — New Service Plans from Bio-Rad’s Service Team https://www.bioradiations.com/bio-rad-expert-care-new-service-plans-from-bio-rads-service-team/ Sun, 11 Aug 2013 17:53:37 +0000 http://www.bioradiations.com/?p=3599 A high quality lab instrument represents a major investment for any researcher. With its new Expert Care service program, Bio-Rad now offers a slate of service plans so its customers can rest assured that their valuable equipment will receive prompt, high-quality support and service at the right level for their needs. Learn about the different options available under the Expert Care service program.

The post Bio-Rad Expert Care — New Service Plans from Bio-Rad’s Service Team first appeared on Bio-Radiations.]]> Using Droplet Digital™ PCR to Study Stem Cell Genomes at Stanford University https://www.bioradiations.com/using-droplet-digital-pcr-to-study-stem-cell-genomes-at-stanford-university/ Thu, 25 Jul 2013 17:27:36 +0000 http://www.bioradiations.com/?p=4008 In this webinar, Prof. Alexander Urban of the Stanford School of Medicine describes how his lab used the QX200™ Droplet Digital™ PCR System to investigate copy number variation (CNV) in induced pluripotent stem cells (iPSCs) that were being used to study the genetics of autism.

The post Using Droplet Digital™ PCR to Study Stem Cell Genomes at Stanford University first appeared on Bio-Radiations.]]> Can Droplet Digital™ PCR Detect 22q11.2 Deletion Syndrome? https://www.bioradiations.com/can-droplet-digital-pcr-detect-22q11-2-deletion-syndrome/ Thu, 18 Jul 2013 17:32:04 +0000 http://www.bioradiations.com/?p=4013 This presentation by Vicki Hwang details how UC Davis researchers used Droplet Digital PCR (ddPCR™) technology to determine copy number variation (CNV) and characterize the deletion endpoints of the region of chromosome 22 that is deleted in 22q11 deletion syndrome.

The post Can Droplet Digital™ PCR Detect 22q11.2 Deletion Syndrome? first appeared on Bio-Radiations.]]> PrimePCR ddPCR CNV Assay Validation Data https://www.bioradiations.com/primepcr-ddpcr-cnv-assay-validation-data/ Tue, 09 Jul 2013 21:46:29 +0000 http://www.bioradiations.com/?p=6334 Gene Name BRCA1 Gene Symbol BRCA1 Species Human Summary breast cancer 1, early onset [Source:HGNC Symbol;Acc:1100] Gene Aliases BRCC1:PPP1R53:RNF53 RefSeq Accession No. NM_007294.3:NM_007299.3:NM_007297.3:NM_007300.3 UniGeneID Hs.194143 Ensemble Gene ID ENSG00000012048 Entrez ...

The post PrimePCR ddPCR CNV Assay Validation Data first appeared on Bio-Radiations.]]> Guide to SPR Data Analysis on the ProteOn™ XPR36 System https://www.bioradiations.com/guide-to-spr-data-analysis-on-the-proteon-xpr36-system/ Tue, 09 Jul 2013 19:00:28 +0000 http://www.bioradiations.com/?p=1505 Analyzing protein interaction data using a surface plasmon resonance (SPR) system can be complicated. This user guide describes how to analyze SPR data to obtain kinetic and equilibrium constants, as well as sample concentrations.

The post Guide to SPR Data Analysis on the ProteOn™ XPR36 System first appeared on Bio-Radiations.]]> Vote Yes for Better Chromatography — The Results Are In! https://www.bioradiations.com/vote-yes-for-better-chromatography-the-results-are-in/ Tue, 09 Jul 2013 19:00:16 +0000 http://www.bioradiations.com/?p=2231 Watch the protein purification election night special parody! Find out which chromatography system wins. Laugh Out Loud at the terrible protein purification "pundit" and Vote Yes for Better Chromatography "Fanatics." Learn what values and system features were appreciated most by voters.

The post Vote Yes for Better Chromatography — The Results Are In! first appeared on Bio-Radiations.]]> New PrimePCR™ Probe Assays for Real-time PCR and Droplet Digital™ PCR Technologies https://www.bioradiations.com/new-primepcr-probe-assays-for-real-time-pcr-and-droplet-digital-pcr-technologies/ Tue, 09 Jul 2013 18:26:52 +0000 http://www.bioradiations.com/?p=3613 Bio-Rad’s PrimePCR probe assays gave researchers a powerful, reliable set of investigative tools utilizing SYBR® Green detection chemistry. Now Bio-Rad is adding a number of new assays to the PrimePCR portfolio, bringing the benefits of PrimePCR assays to many new applications for both real-time PCR and Droplet Digital™ PCR platforms.

The post New PrimePCR™ Probe Assays for Real-time PCR and Droplet Digital™ PCR Technologies first appeared on Bio-Radiations.]]> The Chromatography Chronicles Part 1: The Votes Are in — Customer Voices and the NGC Chromatography System Design Process https://www.bioradiations.com/the-chromatography-chronicles-part-1-the-votes-are-in-customer-voices-and-the-ngc-chromatography-system-design-process/ Tue, 09 Jul 2013 17:47:34 +0000 http://www.bioradiations.com/?p=1107 Whether through unreliability, lack of workflows, or poor design, chromatography systems can cause major hassles for any lab involved in purifying proteins. Would Bio-Rad be able to solve these longstanding problems? This new series takes an inside look at the development of the NGC medium-pressure chromatography system. This month: bringing customers into the design process.

The post The Chromatography Chronicles Part 1: The Votes Are in — Customer Voices and the NGC Chromatography System Design Process first appeared on Bio-Radiations.]]> Options for Dataset Export Using the ProteOn Manager™ Software https://www.bioradiations.com/options-for-dataset-export-using-the-proteon-manager-software/ Tue, 09 Jul 2013 16:34:56 +0000 http://www.bioradiations.com/?p=6297 This appendix to “Guide to SPR Data Analysis on the Proteon™ XPR36 System” gives information on the data set export using ProteOn Manager™ Software.

The post Options for Dataset Export Using the ProteOn Manager™ Software first appeared on Bio-Radiations.]]> Digital Quantitation of Potential Therapeutic Target RNAs https://www.bioradiations.com/digital-quantitation-of-potential-therapeutic-target-rnas/ Mon, 01 Jul 2013 17:36:47 +0000 http://www.bioradiations.com/?p=4019 In this webinar, Dr. David Dodd describes how he and Prof. David Corey and colleagues at UT Southwestern Medical Center have used Droplet Digital™ PCR (ddPCR™) in their research into low-abundance targets with therapeutic potential for cancer and Huntington’s disease.

The post Digital Quantitation of Potential Therapeutic Target RNAs first appeared on Bio-Radiations.]]> Revealing BRCA2 Pathways in Cancer with Bio-Rad’s V3 Western Workflow™ https://www.bioradiations.com/revealing-brca2-pathways-in-cancer-with-bio-rads-v3-western-workflow-video/ Thu, 13 Jun 2013 19:00:34 +0000 http://www.bioradiations.com/?p=2233 Research led by Prof. Ryan Jensen at Yale Medical School focuses on elucidating the links between BRCA gene mutations and breast cancer. Learn how researchers in his lab use Bio-Rad's V3 Western Workflow to study interactions between BRCA proteins and the DNA repair protein RAD51. Understanding how these proteins interact in carcinogenesis and tumor growth will provide a new understanding of breast cancer mechanisms that could lead to new therapies.

The post Revealing BRCA2 Pathways in Cancer with Bio-Rad’s V3 Western Workflow™ first appeared on Bio-Radiations.]]> Revealing BRCA2 Pathways in Cancer with Bio-Rad’s V3 Western Workflow https://www.bioradiations.com/revealing-brca2-pathways-in-cancer-with-bio-rads-v3-western-workflow/ Thu, 23 May 2013 01:42:29 +0000 http://www.bioradiations.com/?p=816 Twenty years after the discovery of the link between BRCA gene mutations and breast cancer, researcher Ryan Jensen of the Yale Medical School is looking into the interactions between the DNA repair protein RAD51 and BRCA proteins using Bio-Rad’s V3 Western Workflow.

The post Revealing BRCA2 Pathways in Cancer with Bio-Rad’s V3 Western Workflow first appeared on Bio-Radiations.]]> Reliable, Streamlined 2-D Western Blot Workflow for Evaluation of Antibodies Developed for Detection of Host Cell Proteins https://www.bioradiations.com/reliable-streamlined-2-d-western-blot-workflow-for-evaluation-of-antibodies-developed-for-detection-of-host-cell-proteins/ Fri, 17 May 2013 19:00:31 +0000 http://www.bioradiations.com/?p=1397 Removing host cell protein (HCP) contaminants represents an essential step in the production of biologics (therapeutic protein drugs), but has long been a time- and labor-intensive process. Here, we demonstrate a simpler, quicker, and more standardized workflow for evaluating anti-HCP antibodies using 2-D electrophoresis and western blotting.

The post Reliable, Streamlined 2-D Western Blot Workflow for Evaluation of Antibodies Developed for Detection of Host Cell Proteins first appeared on Bio-Radiations.]]> T100™ Thermal Cycler – Endorsed by Customers around the World https://www.bioradiations.com/t100-thermal-cycler-endorsed-by-customers-around-the-world/ Thu, 25 Apr 2013 19:00:20 +0000 http://www.bioradiations.com/?p=2235 Life science researchers all over the world use the T100 thermal cycler in their daily routine. In this video, users describe how it saves them time and helps them consistently obtain high-quality data.

The post T100™ Thermal Cycler – Endorsed by Customers around the World first appeared on Bio-Radiations.]]> Bio-Rad’s Applications & Technologies Pages: A Valuable Resource for Scientists https://www.bioradiations.com/bio-rads-applications-and-technologies-pages-a-valuable-resource-for-scientists/ Tue, 23 Apr 2013 22:39:24 +0000 http://www.bioradiations.com/?p=1602 Discover the wide range of applications for Bio-Rad products in diverse fields of study and learn more about our cutting-edge technologies and their underlying principles. Get protocols, troubleshooting tips, and updates on the latest developments in methods, instrumentation, and software.

The post Bio-Rad’s Applications & Technologies Pages: A Valuable Resource for Scientists first appeared on Bio-Radiations.]]> Using Bio-Rad’s PDQuest Software to Generate a Match Rate between 2-D Electrophoresis and Western Blotting https://www.bioradiations.com/using-bio-rads-pdquest-software-to-generate-a-match-rate-between-2-d-electrophoresis-and-western-blotting/ Tue, 23 Apr 2013 01:00:00 +0000 http://www.bioradiations.com/?p=964 2-D electrophoresis followed by immunodetection of protein spots using western blotting is adopted commonly in proteomics studies. Here we present step-by-step instructions for obtaining accurate overlaps between the gel and the blot using the PDQuest 2-D gel analysis software.

The post Using Bio-Rad’s PDQuest Software to Generate a Match Rate between 2-D Electrophoresis and Western Blotting first appeared on Bio-Radiations.]]> New and Improved Microplate Manager® Software for Microplate Data Acquisition and Analysis https://www.bioradiations.com/new-and-improved-microplate-manager-software-for-microplate-data-acquisition-and-analysis/ Wed, 27 Mar 2013 17:16:23 +0000 http://www.bioradiations.com/?p=3637 Bio-Rad’s microplate readers function at their full potential when used with Microplate Manager Software. This updated version of the software features two new read modes, compatibility with the latest operating systems, and more.

The post New and Improved Microplate Manager® Software for Microplate Data Acquisition and Analysis first appeared on Bio-Radiations.]]> Real-Time PCR Interactive Tutorials — From Reagent Selection to Real-Time PCR Data Analysis https://www.bioradiations.com/real-time-pcr-interactive-tutorials-from-reagent-selection-to-real-time-pcr-data-analysis/ Tue, 26 Mar 2013 22:42:42 +0000 http://www.bioradiations.com/?p=1712 Obtaining accurate quantitative PCR data depends on choosing and validating the proper reagents for a given experiment. These new tutorials present the knowledge base needed to understand the parameters involved in reagent selection in order to get the best results from real-time PCR procedures.

The post Real-Time PCR Interactive Tutorials — From Reagent Selection to Real-Time PCR Data Analysis first appeared on Bio-Radiations.]]> Conservation Genetics: High School Students Use Biotechnology Explorer™ Products to Help Save California Redwoods https://www.bioradiations.com/conservation-genetics-high-school-students-use-biotechnology-explorer-products-to-help-save-california-redwoods/ Thu, 21 Mar 2013 21:58:34 +0000 http://www.bioradiations.com/?p=1511 “If we want students to be biologists, let’s start being biologists,” says Ray Cinti, a science teacher at Convent of the Sacred Heart High School in San Francisco. Find out how Cinti’s class is accomplishing this by doing real research on Coast Redwoods genetic diversity using the Biotechnology Explorer Cloning and Sequencing Explorer series.

The post Conservation Genetics: High School Students Use Biotechnology Explorer™ Products to Help Save California Redwoods first appeared on Bio-Radiations.]]> Bio-Rad’s 2-D Electrophoresis Workflow How-To Guide, Fourth Edition, is Now Available for Download https://www.bioradiations.com/bio-rads-2-d-electrophoresis-workflow-how-to-guide-fourth-edition-is-now-available-for-download/ Tue, 19 Mar 2013 17:57:28 +0000 http://www.bioradiations.com/?p=3441 Bio-Rad’s 2-D electrophoresis workflow offers better results in a shorter time. Take a detailed look at this workflow by downloading the updated edition of our How-To Guide.

The post Bio-Rad’s 2-D Electrophoresis Workflow How-To Guide, Fourth Edition, is Now Available for Download first appeared on Bio-Radiations.]]> Digital PCR at the Next Level: Users Find Many Applications for the QX100™ Droplet Digital™ PCR System https://www.bioradiations.com/digital-pcr-at-the-next-level-users-find-many-applications-for-the-qx100-droplet-digital-pcr-system/ Tue, 12 Mar 2013 19:00:55 +0000 http://www.bioradiations.com/?p=2610 The QX100 Droplet Digital PCR system gives researchers an improvement in PCR precision and sensitivity. See how this innovative digital PCR system is enabling companies to create better products, improve workflow efficiencies, and validate results with greater accuracy than previous methods allowed.

The post Digital PCR at the Next Level: Users Find Many Applications for the QX100™ Droplet Digital™ PCR System first appeared on Bio-Radiations.]]> Learning in their DNA: Inside the Bio-Rad Science Ambassador Program https://www.bioradiations.com/learning-in-their-dna-inside-the-bio-rad-science-ambassador-program/ Tue, 12 Mar 2013 18:17:27 +0000 http://www.bioradiations.com/?p=1565 Take a closer look at Bio-Rad's initiative to bring scientists into K–12 schools in North America by following three Ambassadors as they go into classrooms. Find out more about what this program offers students, teachers, and researchers.

The post Learning in their DNA: Inside the Bio-Rad Science Ambassador Program first appeared on Bio-Radiations.]]> PrimePCR™ PCR Primers & Assays https://www.bioradiations.com/primepcr-pcr-primers-assays/ Sat, 02 Mar 2013 00:04:49 +0000 http://www.bioradiations.com/?p=5768 PrimePCR™ assays for real-time PCR and Droplet Digital™ PCR are expertly designed and experimentally validated for guaranteed performance. Ways to order PrimePCR assays: My PrimePCR Reorder previously ordered assays PrimePCR ...

The post PrimePCR™ PCR Primers & Assays first appeared on Bio-Radiations.]]> Investigating Protein Translation with the NGC™ Chromatography System https://www.bioradiations.com/investigating-protein-translation-with-the-ngc-chromatography-system/ Thu, 14 Feb 2013 20:00:55 +0000 http://www.bioradiations.com/?p=2237 The Fraser lab at University of California Davis tested Bio-Rad’s NGC system for the purification of ribosomal complex proteins in their studies of protein translation mechanisms. The NGC system enabled these researchers to significantly increase their protein purification throughput and save valuable time in the lab.

The post Investigating Protein Translation with the NGC™ Chromatography System first appeared on Bio-Radiations.]]> A Novel Biotinylated Ligand-Capture Method with Surface Regeneration Capability for Label-Free Biomolecular Interaction Analysis https://www.bioradiations.com/a-novel-biotinylated-ligand-capture-method-with-surface-regeneration-capability-for-label-free-biomolecular-interaction-analysis/ Tue, 12 Feb 2013 20:00:47 +0000 http://www.bioradiations.com/?p=1486 Ligand-capture approaches using biotin as an affinity tag are often utilized in biomolecular interaction analyses with surface plasmon resonance (SPR) biosensors. However, a drawback to using biotin is that it makes surface regeneration of SPR sensor chips difficult. Here is a strategy that facilitates surface regeneration when using biotinylated ligand-capture approaches to SPR analysis.

The post A Novel Biotinylated Ligand-Capture Method with Surface Regeneration Capability for Label-Free Biomolecular Interaction Analysis first appeared on Bio-Radiations.]]> Investigating Protein Translation with Bio-Rad’s NGC Chromatography System https://www.bioradiations.com/investigating-protein-translation-with-bio-rads-ngc-chromatography-system/ Fri, 08 Feb 2013 04:51:44 +0000 http://www.bioradiations.com/?p=666 The Fraser Laboratory in the Dept. of Molecular and Cellular Biology at UC Davis studies protein translation mechanisms by purifying diverse ribosomal proteins and reconstructing the translation complex in vitro. The lab utilized Bio-Rad’s NGC system to overcome specific purification difficulties with their protein while beta-testing the equipment. The NGC system enabled the group to increase their throughput by 25% and also saved substantial training time.

The post Investigating Protein Translation with Bio-Rad’s NGC Chromatography System first appeared on Bio-Radiations.]]> Amplifying in the Outback: Researcher Brings Real-Time PCR to Australia’s Kimberley Wilderness https://www.bioradiations.com/amplifying-in-the-outback-researcher-brings-real-time-pcr-to-australias-kimberley-wilderness/ Mon, 04 Feb 2013 21:22:23 +0000 http://www.bioradiations.com/?p=3294 Dr. Tim Inglis of the University of Western Australia seeks better ways to conduct surveillance and respond to outbreaks of tropical infectious diseases. For over a decade he’s been developing detection assays for a slate of pathogens. Find out how Bio-Rad’s MiniOpticon Real-Time PCR system allows him to effectively run these assays in such remote locations as the Kimberley Wilderness in Australia’s Top End.

The post Amplifying in the Outback: Researcher Brings Real-Time PCR to Australia’s Kimberley Wilderness first appeared on Bio-Radiations.]]> Immobilization of Active Kinases for Small Molecule Inhibition Studies https://www.bioradiations.com/immobilization-of-active-kinases-for-small-molecule-inhibition-studies/ Mon, 04 Feb 2013 19:46:31 +0000 http://www.bioradiations.com/?p=1340 Surface plasmon resonance (SPR) is a well-established and important screening tool in the small molecule drug discovery workflow. Using the Bio-Rad SPR system ProteOn XPR36, rapid optimization of immobilization conditions for a kinase target was carried out in a fast workflow with a single sensor chip, showing the high performance of the ProteOn XPR36 system.

The post Immobilization of Active Kinases for Small Molecule Inhibition Studies first appeared on Bio-Radiations.]]> Bio-Rad Science Ambassador Program Pairs Scientists and Schools — Helps Kids Get Into Science https://www.bioradiations.com/bio-rad-science-ambassador-program-pairs-scientists-and-schools-helps-kids-get-into-science/ Tue, 08 Jan 2013 22:10:43 +0000 http://www.bioradiations.com/?p=1854 Following a successful pilot effort in early 2012, Bio-Rad is proud to announce the official launch of the Bio-Rad Science Ambassador Program, which empowers scientists to engage directly with local schools to build student interest in the sciences and increase access to quality science education.

The post Bio-Rad Science Ambassador Program Pairs Scientists and Schools — Helps Kids Get Into Science first appeared on Bio-Radiations.]]> Gel Doc™ EZ Imaging System — Push-Button Imaging in Just Ten Seconds https://www.bioradiations.com/gel-doc-ez-imaging-system-push-button-imaging-in-just-ten-seconds/ Tue, 08 Jan 2013 19:30:02 +0000 http://www.bioradiations.com/?p=3453 Bio-Rad’s innovative Gel Doc EZ imaging system features something unique that no other imaging system has – brains.

The post Gel Doc™ EZ Imaging System — Push-Button Imaging in Just Ten Seconds first appeared on Bio-Radiations.]]> Analyzing Binding Kinetics with Surface Plasmon Resonance Complemented with Direct Mass Spectrometry on the Same Sensor Chip https://www.bioradiations.com/analyzing-binding-kinetics-with-surface-plasmon-resonance-complimented-with-direct-mass-spectrometry-on-the-same-sensor-chip/ Mon, 07 Jan 2013 20:00:23 +0000 http://www.bioradiations.com/?p=1465 Surface Plasmon Resonance (SPR) is an optical phenomenon that is used for the label-free analysis of the binding of any two molecules in real-time.

The post Analyzing Binding Kinetics with Surface Plasmon Resonance Complemented with Direct Mass Spectrometry on the Same Sensor Chip first appeared on Bio-Radiations.]]> New Premium Hard-Shell Microplate for Applied Biosystems 7500 Fast and ViiA 7 Fast Real-Time PCR systems https://www.bioradiations.com/new-premium-hard-shell-microplate-for-applied-biosystems-7500-fast-and-viia-7-fast-real-time-pcr-systems/ Mon, 07 Jan 2013 18:33:35 +0000 http://www.bioradiations.com/?p=3649 Bio-Rad introduces new Hard-Shell 96-well Semi-skirted low profile PCR plates for optimal performance with a variety of real-time PCR systems.

The post New Premium Hard-Shell Microplate for Applied Biosystems 7500 Fast and ViiA 7 Fast Real-Time PCR systems first appeared on Bio-Radiations.]]> Top Stories of 2012 – BioRadiations Year in Review https://www.bioradiations.com/top-stories-of-2012-bioradiations-year-in-review/ Fri, 14 Dec 2012 20:00:36 +0000 http://www.bioradiations.com/?p=4176 Bioradiations.com, the online technical resource for Bio-Rad customers, has been offering a variety of research tips and protocols, customer stories and testimonials, and in-depth technical reports throughout the year. Here are the highlights of what we presented in the year 2012.

The post Top Stories of 2012 – BioRadiations Year in Review first appeared on Bio-Radiations.]]> New Roche LightCycler 480 Hard-Shell® Plate That Does Not Warp During Thermal Cycling https://www.bioradiations.com/new-roche-lightcycler-480-hard-shell-plate-that-does-not-warp-during-thermal-cycling/ Fri, 14 Dec 2012 19:15:12 +0000 http://www.bioradiations.com/?p=3669 Stability during thermal cycling and uniformity of wells are important features in any PCR plate for obtaining reliable results. Bio-Rad’s new Hard-Shell® plates are stable, prevent warping and shrinkage, and are designed for optimal thermal transfer and recovery of samples.

The post New Roche LightCycler 480 Hard-Shell® Plate That Does Not Warp During Thermal Cycling first appeared on Bio-Radiations.]]> ChemiDoc™ MP Imaging System Testimonial — KAIST, South Korea (full version) https://www.bioradiations.com/chemidoc-mp-imaging-system-testimonial-kaist-south-korea-full-version/ Wed, 14 Nov 2012 20:00:03 +0000 http://www.bioradiations.com/?p=2239 Learn how scientists in the Gene Regulation Laboratory at the Korea Advanced Institute of Science and Technology (KAIST) use the ChemiDoc MP imaging system in their work investigating the molecular machinery involved in epigenetic gene regulation.

The post ChemiDoc™ MP Imaging System Testimonial — KAIST, South Korea (full version) first appeared on Bio-Radiations.]]> Bio-Rad’s New S3™ Cell Sorter Automates and Simplifies Cell Sorting https://www.bioradiations.com/bio-rads-new-s3-cell-sorter-automates-and-simplifies-cell-sorting/ Mon, 29 Oct 2012 19:11:32 +0000 http://www.bioradiations.com/?p=3693 Cell sorting is a complex and expensive process, with complicated steps requiring training. Bio-Rad’s new S3™ cell sorter is compact, easy to use, automated, affordable, and provides exceptional sort purity and yield results.

The post Bio-Rad’s New S3™ Cell Sorter Automates and Simplifies Cell Sorting first appeared on Bio-Radiations.]]> New CFX96 Touch™ Deep Well Real-Time PCR Instrument Enables Large-Volume qPCR Reactions https://www.bioradiations.com/new-cfx96-touch-deep-well-real-time-pcr-instrument-enables-large-volume-qpcr-reactions/ Sun, 28 Oct 2012 20:32:09 +0000 http://www.bioradiations.com/?p=3709 Large sample volumes may sometimes be needed to conduct gene expression studies. Bio-Rad’s new CFX96 Touch Deep Well™ real-time PCR detection system can run sample volumes up to 125 µl and can discriminate up to five targets simultaneously.

The post New CFX96 Touch™ Deep Well Real-Time PCR Instrument Enables Large-Volume qPCR Reactions first appeared on Bio-Radiations.]]> CFX Qualification Plate — A Reliable Performance Verification Tool for any CFX Real-Time PCR System https://www.bioradiations.com/cfx-qualification-plate-a-reliable-performance-verification-tool-for-any-cfx-real-time-pcr-system/ Fri, 26 Oct 2012 18:28:28 +0000 http://www.bioradiations.com/?p=3771 Verifying the performance of your real-time PCR system is crucial to obtaining reliable data from your experiments. Bio-Rad’s new CFX qualification plate comes with a quick and easy protocol that provides reliable performance verification of any real-time qPCR system.

The post CFX Qualification Plate — A Reliable Performance Verification Tool for any CFX Real-Time PCR System first appeared on Bio-Radiations.]]> PrimePCR™ Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy https://www.bioradiations.com/primepcr-pathway-analysis-pathway-curation-and-real-time-pcr-panel-design-strategy/ Wed, 24 Oct 2012 19:00:31 +0000 http://www.bioradiations.com/?p=1687 Pathway analysis is an efficient approach for studying a large number of related gene targets in a single experiment. Bio-Rad Laboratories and Thomson Reuters, a leading provider of system biology tools, have partnered to provide predesigned pathway panels for gene expression analyses using real-time qPCR. This tech report describes how Bio-Rad’s PrimePCR™ pathway panels were curated and designed using a ranking strategy developed by Thomson Reuters.

The post PrimePCR™ Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy first appeared on Bio-Radiations.]]> New Semi-Automated Heat Sealer Delivers Consistent and Reliable PCR Plate Sealing https://www.bioradiations.com/new-semi-automated-heat-sealer-delivers-consistent-and-reliable-pcr-plate-sealing/ Mon, 08 Oct 2012 19:09:57 +0000 http://www.bioradiations.com/?p=3806 The PX1 PCR Plate Sealer efficiently seals microplates used in thermal cycling. This heat sealing method consistently delivers reliable data by helping eliminate sample evaporation during PCR.

The post New Semi-Automated Heat Sealer Delivers Consistent and Reliable PCR Plate Sealing first appeared on Bio-Radiations.]]> TGX Stain-Free™ Precast Gels Facilitate LDL Research on Potential New Drug Targets https://www.bioradiations.com/tgx-stain-free-precast-gels-facilitate-ldl-research-on-potential-new-drug-targets/ Mon, 01 Oct 2012 17:53:48 +0000 http://www.bioradiations.com/?p=1057 When Dr. Fred Bauzon started his research, he worked with bioinformatics specialists to functionalize suitable gene candidates based on their expression levels with HMGCR transcript levels. All his experiments demonstrated a reduction in low-density lipoprotein receptor protein (LDLR) and candidate gene protein levels after the candidate gene was silenced. Here Bauzon discusses how TGX Stain-free precast gels have aided his research toward future drug targets for the therapeutic control of LDL uptake.

The post TGX Stain-Free™ Precast Gels Facilitate LDL Research on Potential New Drug Targets first appeared on Bio-Radiations.]]> ChemiDoc™ MP Imaging System Testimonial — Erlangen, Germany (full version) https://www.bioradiations.com/chemidoc-mp-imaging-system-testimonial-erlangen-germany-full-version/ Fri, 14 Sep 2012 19:00:29 +0000 http://www.bioradiations.com/?p=2241 Learn how scientists in the Department of Animal Physiology at the University of Erlangen in Erlangen, Germany use the ChemiDoc MP imaging system in their work investigating sensory systems, in particular, the eye.

The post ChemiDoc™ MP Imaging System Testimonial — Erlangen, Germany (full version) first appeared on Bio-Radiations.]]> New TC20™ Automated Cell Counter Facilitates Quick Counting with Precision https://www.bioradiations.com/new-tc20-automated-cell-counter-facilitates-quick-counting-with-precision/ Wed, 05 Sep 2012 20:10:10 +0000 http://www.bioradiations.com/?p=3838 Bio-Rad’s new TC20 automated cell counter is compatible with broad range of cell sizes and types such as cell lines, primary cells (from tissue or blood), and stem cells.

The post New TC20™ Automated Cell Counter Facilitates Quick Counting with Precision first appeared on Bio-Radiations.]]> Expect More From Your Chemiluminescent Substrate: Introducing Clarity™ Western ECL Substrate https://www.bioradiations.com/expect-more-from-your-chemiluminescent-substrate-introducing-clarity-western-ecl-substrate/ Tue, 04 Sep 2012 17:08:06 +0000 http://www.bioradiations.com/?p=3980 Bio-Rad’s new Clarity Western ECL substrate provides high sensitivity, long signal duration, and low background even at long exposures.

The post Expect More From Your Chemiluminescent Substrate: Introducing Clarity™ Western ECL Substrate first appeared on Bio-Radiations.]]> Trans-Blot® Turbo™ Ready-to-Assemble (RTA) Kit Enables Economical, Rapid, and Efficient Western Blot Transfers https://www.bioradiations.com/trans-blot-turbo-ready-to-assemble-rta-kit-enables-economical-rapid-and-efficient-western-blot-transfers/ Mon, 03 Sep 2012 17:33:49 +0000 http://www.bioradiations.com/?p=4014 Bio-Rad’s latest semi-dry transfer system, the Trans-Blot® Turbo™, has changed the way researchers think about western blot transfers.

The post Trans-Blot® Turbo™ Ready-to-Assemble (RTA) Kit Enables Economical, Rapid, and Efficient Western Blot Transfers first appeared on Bio-Radiations.]]> PrimePCR™ Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation https://www.bioradiations.com/primepcr-assays-meeting-the-miqe-guidelines-by-full-wet-lab-validation/ Mon, 20 Aug 2012 19:00:20 +0000 http://www.bioradiations.com/?p=1659 The MIQE guidelines have set a new standard for publishing qPCR results to ensure integrity in the scientific literature and increase experimental transparency. One of the key guidelines focuses on the proper design and experimental validation of primer assays used in qPCR. This tech report describes the design and wet-lab validation of PrimePCR assays and how they comply with the MIQE guidelines.

The post PrimePCR™ Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation first appeared on Bio-Radiations.]]> High Sensitivity of Bio-Plex® Multiplex System is Key in Study for Novel Melanoma Therapeutics https://www.bioradiations.com/high-sensitivity-of-bio-plex-multiplex-system-is-key-in-study-for-novel-melanoma-therapeutics/ Wed, 15 Aug 2012 21:43:36 +0000 http://www.bioradiations.com/?p=1176 alt

Melanoma is an aggressive cancer where early detection is essential for patient survival. The Facchiano lab is developing new anti-proliferation molecules against melanoma and is working to identify new molecular markers at the very early stages of pathology. Here Dr Antonio Facchiano discusses the merits of using the Bio-Plex technology to measure dose-dependent changes in expression levels of growth and angiogenic factors in cell lysates and supernatants.

The post High Sensitivity of Bio-Plex® Multiplex System is Key in Study for Novel Melanoma Therapeutics first appeared on Bio-Radiations.]]> Bio-Plex® Multiplex System is Key in Novel Melanoma Therapeutics Study https://www.bioradiations.com/bio-plex-multiplex-system-is-key-in-novel-melanoma-therapeutics-study/ Wed, 15 Aug 2012 19:00:33 +0000 http://www.bioradiations.com/?p=2245 Researchers at the Immaculate Dermopathic Institute (IDI-IRCCS, Rome) are using Bio-Plex technology in developing new anti-proliferation molecules and markers at the early stages of melanoma pathology. Dr Antonio Facchiano discusses how Bio-Plex has expedited the lab’s research.

The post Bio-Plex® Multiplex System is Key in Novel Melanoma Therapeutics Study first appeared on Bio-Radiations.]]> New and Improved CFX Manager™ Software for Real-Time PCR Data Acquisition and Analysis https://www.bioradiations.com/new-and-improved-cfx-manager-software-for-real-time-pcr-data-acquisition-and-analysis/ Mon, 06 Aug 2012 17:49:28 +0000 http://www.bioradiations.com/?p=4035 The latest version of the CFX Manager software provides a variety of usability enhancements and powerful new data analyses so users can get the most out of each run.

The post New and Improved CFX Manager™ Software for Real-Time PCR Data Acquisition and Analysis first appeared on Bio-Radiations.]]> PDQuest™ Software: Bite-Sized Video Tutorials Make Learning 2-D Analysis Easy https://www.bioradiations.com/pdquest-software-bite-sized-video-tutorials-make-learning-2-d-analysis-easy/ Wed, 18 Jul 2012 19:10:36 +0000 http://www.bioradiations.com/?p=3478 Learning to use analysis software can be time-consuming and following a manual can be challenging.

The post PDQuest™ Software: Bite-Sized Video Tutorials Make Learning 2-D Analysis Easy first appeared on Bio-Radiations.]]> PDQuest™ Software Tutorials https://www.bioradiations.com/pdquest-software-tutorials/ Wed, 18 Jul 2012 19:00:24 +0000 http://www.bioradiations.com/?p=2248 The nine PDQuest tutorials are chapterized so you can focus on what you want to learn. Each video runs from 3 to 9 minutes and features callout boxes that highlight important information to complement the audio explanations.

The post PDQuest™ Software Tutorials first appeared on Bio-Radiations.]]> QX100™ Droplet Digital™ ddPCR™ System at Sangamo BioSciences https://www.bioradiations.com/qx100-droplet-digital-ddpcr-system-at-sangamo-biosciences/ Fri, 13 Jul 2012 22:36:02 +0000 http://www.bioradiations.com/?p=2250 Researchers at Sangamo BioSciences, Inc. explain the benefits of using third generation PCR as it applies to their research in the field of personalized medicine. Watch to see the latest generation of quantitative PCR in action.

The post QX100™ Droplet Digital™ ddPCR™ System at Sangamo BioSciences first appeared on Bio-Radiations.]]> New Gradient Format Mini-PROTEAN® TGX Stain-Free™ Precast Gels for SDS-PAGE — Streamlining Electrophoresis and Blotting Workflows https://www.bioradiations.com/new-gradient-format-mini-protean-tgx-stain-free-precast-gels-for-sds-page-streamlining-electrophoresis-and-blotting-workflows/ Fri, 13 Jul 2012 18:21:28 +0000 http://www.bioradiations.com/?p=4054 Mini-PROTEAN® TGX Stain-Free™ precast gels are now available in gradient format. This unique gel formation allows for fast gel electrophoresis and blotting, and does not require staining and destaining for imaging.

The post New Gradient Format Mini-PROTEAN® TGX Stain-Free™ Precast Gels for SDS-PAGE — Streamlining Electrophoresis and Blotting Workflows first appeared on Bio-Radiations.]]> Experimentally Validated PrimePCR™ Assays and Panels for Real-Time PCR https://www.bioradiations.com/experimentally-validated-primepcr-assays-and-panels-for-real-time-pcr/ Fri, 29 Jun 2012 19:04:35 +0000 http://www.bioradiations.com/?p=4068 PrimePCR™ assays set a new standard for real-time PCR. Bio-Rad® collaborated with the leaders in real-time PCR to design, optimize, and experimentally validate every primer pair.

The post Experimentally Validated PrimePCR™ Assays and Panels for Real-Time PCR first appeared on Bio-Radiations.]]> Droplet Digital PCR Opens New Perspectives in HIV Research https://www.bioradiations.com/droplet-digital-pcr-opens-new-perspectives-in-hiv-research/ Fri, 15 Jun 2012 22:42:23 +0000 http://www.bioradiations.com/?p=1040 Sangamo BioSciences, Inc., located in Richmond, California, is developing HIV therapeutics toward a functional cure using their proprietary zinc finger DNA-binding protein technology. Droplet digital PCR (ddPCR™) — or third generation PCR — offers a superior solution as it can detect target sequence within a single molecule, making it a powerful technology for low copy event detection.

The post Droplet Digital PCR Opens New Perspectives in HIV Research first appeared on Bio-Radiations.]]> Image Lab™ 4.1, the Latest Software Version, Addresses the Challenges of Multiplex Analysis and Protein Normalization https://www.bioradiations.com/image-lab-4-1-the-latest-software-version-addresses-the-challenges-of-multiplex-analysis-and-protein-normalization/ Wed, 13 Jun 2012 20:19:20 +0000 http://www.bioradiations.com/?p=4094 The updated Image Lab image acquisition and analysis software provides improved tools for total protein normalization using the stain-free technology.

The post Image Lab™ 4.1, the Latest Software Version, Addresses the Challenges of Multiplex Analysis and Protein Normalization first appeared on Bio-Radiations.]]> Bio-Rad’s New 4x Laemmli Sample Buffer for SDS-PAGE https://www.bioradiations.com/bio-rads-new-4x-laemmli-sample-buffer-for-sds-page/ Tue, 12 Jun 2012 16:57:31 +0000 http://www.bioradiations.com/?p=4108 Detecting dilute protein samples using standard protein gel stains can be problematic. One method of enabling increased sample capacity on gels is to increase the well size. The newly introduced 4x Laemmli sample buffer improves sample loading capacity, improving the detection of low-abundance proteins.

The post Bio-Rad’s New 4x Laemmli Sample Buffer for SDS-PAGE first appeared on Bio-Radiations.]]> Video Tutorial for Analyzing Binding Interactions with Histidine-Tagged Proteins https://www.bioradiations.com/analyzing-binding-interactions/ Tue, 22 May 2012 19:00:35 +0000 http://www.bioradiations.com/?p=1564 Analyzing antibody binding to histidine-tagged proteins is not always easy because of inherent problems such as nonspecific binding and loss of analyte response associated with these tagged proteins. Optimization of these factors is key to precisely measuring the binding kinetics of these proteins.

The post Video Tutorial for Analyzing Binding Interactions with Histidine-Tagged Proteins first appeared on Bio-Radiations.]]> V3 Western Workflow™ https://www.bioradiations.com/v3-western-workflow/ Fri, 11 May 2012 19:00:44 +0000 http://www.bioradiations.com/?p=2254 Bio-Rad’s five-step western blotting workflow incorporates traditional blotting techniques with innovative technology, allowing quick confirmation of gel runs and blot transfers, and simplifying protein normalization. See how it works.

The post V3 Western Workflow™ first appeared on Bio-Radiations.]]> Novel V3 Western Workflow™ Revolutionizes Protein Research https://www.bioradiations.com/novel-v3-western-workflow-revolutionizes-protein-research/ Thu, 10 May 2012 19:00:24 +0000 http://www.bioradiations.com/?p=4259 Although the Western blot is a core lab procedure, surprisingly little has changed in traditional bench work practice since its inception in 1979. The V3 Western Workflow delivers a streamlined solution to the conventional western blotting workflow as explored here by one protein researcher specializing in chronic obstructive pulmonary disease (COPD).

The post Novel V3 Western Workflow™ Revolutionizes Protein Research first appeared on Bio-Radiations.]]> Focus on the Biology: Bio-Rad’s New Bio-Plex Data Pro™ Software Simplifies Multiplex Data Analysis https://www.bioradiations.com/focus-on-the-biology-bio-rads-new-bio-plex-data-pro-software-simplifies-multiplex-data-analysis/ Fri, 27 Apr 2012 17:28:57 +0000 http://www.bioradiations.com/?p=4121 Many instruments offer multiplexing capability for biomarker measurements. But to handle and make sense of the huge amount of multiplex data requires sophisticated software like Bio-Plex Data Pro, which simplifies organization, import, sorting and visualization of data, and performing calculations and statistical analyses.

The post Focus on the Biology: Bio-Rad’s New Bio-Plex Data Pro™ Software Simplifies Multiplex Data Analysis first appeared on Bio-Radiations.]]> Multiplex Analysis of Inflammatory Markers Using Bio-Plex Pro™ Human Th17 Cytokine Assays https://www.bioradiations.com/multiplex-analysis-of-inflammatory-markers-using-bio-plex-pro-human-th17-cytokine-assays/ Wed, 25 Apr 2012 19:00:03 +0000 http://www.bioradiations.com/?p=1864 The T-helper cell (Th17) pathway has been implicated in several autoimmune diseases and in cancer. New magnetic bead–based assays have been developed for several biomarkers in this pathway. The performance characteristics of these assays are evaluated from data on intra- and inter-assay precision, standard curve recovery, cross reactivity, and linearity of dilution.

The post Multiplex Analysis of Inflammatory Markers Using Bio-Plex Pro™ Human Th17 Cytokine Assays first appeared on Bio-Radiations.]]> The QX100™ Droplet Digital™ PCR System Workflow https://www.bioradiations.com/the-qx100-droplet-digital-pcr-system-workflow/ Mon, 16 Apr 2012 19:00:10 +0000 http://www.bioradiations.com/?p=2256 Watch the step-by-step workflow for absolute quantification of DNA molecules using the third generation droplet digital PCR technology.

The post The QX100™ Droplet Digital™ PCR System Workflow first appeared on Bio-Radiations.]]> Novel HRM Assays Expedite Drug Resistance Surveillance for Leprosy Research https://www.bioradiations.com/novel-hrm-assays-expedite-drug-resistance-surveillance-for-leprosy-research/ Tue, 10 Apr 2012 18:22:14 +0000 http://www.bioradiations.com/?p=3363 The significant drop in leprosy worldwide is attributed to the development of antimicrobial multidrug therapy (MDT). Resistance to rifampicin — the backbone of MDT treatment — has appeared. Controlling leprosy transmission requires routine surveillance for mutations in the drug target genes before, during, and after the course of treatment. New high resolution melt (HRM) assays offer leprosy researchers a faster, more economical method to investigate resistance targets.

The post Novel HRM Assays Expedite Drug Resistance Surveillance for Leprosy Research first appeared on Bio-Radiations.]]> Campus Source Reduction Programs Bolster Environmental Savings: Sustainability Part Three https://www.bioradiations.com/campus-source-reduction-programs-bolster-environmental-savings-sustainability-part-three/ Tue, 10 Apr 2012 17:51:37 +0000 http://www.bioradiations.com/?p=4132 Practicing source reduction — simply creating less waste — helps industries decrease raw material use. Despite improvements in global recycling since the first Earth Day on April 22, 1970, the amount of generated waste has increased substantially. Bio-Rad recognizes that source reduction is essential for conserving natural resources. Highlighted here are several source reduction projects from the Bio-Rad campus in recognition of Earth Day 2012 that complement our mission to provide high-quality products to advance scientific discovery globally.

The post Campus Source Reduction Programs Bolster Environmental Savings: Sustainability Part Three first appeared on Bio-Radiations.]]> A Simple Method for Obtaining Absolute Quantification of DNA Molecules Using the Innovative Droplet Digital™ PCR Technology https://www.bioradiations.com/a-simple-method-for-obtaining-absolute-quantification-of-dna-molecules-using-the-innovative-droplet-digital-pcr-technology/ Thu, 29 Mar 2012 19:18:12 +0000 http://www.bioradiations.com/?p=3484 Droplet digital PCR is considered the third generation of PCR technology. This technology enables the quantification of absolute measure of target DNA molecules, rather than the relative measure obtained from real-time PCR. Absolute quantification is advantageous in several applications, such as measuring copy number variation, detecting rare sequences and mutations, and analyzing gene expression.

The post A Simple Method for Obtaining Absolute Quantification of DNA Molecules Using the Innovative Droplet Digital™ PCR Technology first appeared on Bio-Radiations.]]> Bio-Plex® System in Korea https://www.bioradiations.com/bio-plex-system-in-korea/ Wed, 14 Mar 2012 19:00:31 +0000 http://www.bioradiations.com/?p=2258 Dr Hunseok Lee of Seoul National University explains why it was advantageous for him to use the Bio-Plex system to measure cytokine levels in patients administered with the new cancer vaccine that his laboratory has developed.

The post Bio-Plex® System in Korea first appeared on Bio-Radiations.]]> Solar Power Shines as a Bright Alternative to Fossil Fuel: Sustainability Part Two https://www.bioradiations.com/solar-power-shines-as-a-bright-alternative-to-fossil-fuel-sustainability-part-two/ Sat, 10 Mar 2012 19:24:59 +0000 http://www.bioradiations.com/?p=4154 California, with its abundance of sunshine, has a long history of support for solar energy. For Bio-Rad — headquartered in Hercules, California — solar power made business and environmental sense when considering clean energy alternatives. Bio-Rad’s solar project has proved to be a step in the right direction, mitigating the environmental impact of the company’s energy use, now and in the years to come.

The post Solar Power Shines as a Bright Alternative to Fossil Fuel: Sustainability Part Two first appeared on Bio-Radiations.]]> Green Thinking Sparks New Ideas, Better Programs: Sustainability Part One https://www.bioradiations.com/green-thinking-sparks-new-ideas-better-programs-sustainability-part-one/ Fri, 09 Mar 2012 20:08:03 +0000 http://www.bioradiations.com/?p=4172 Bio-Rad recognizes that reducing shipping associated with consumer products is a direct opportunity to slow greenhouse gas emissions contributing to climate change. Bio-Rad supply centers consolidate shipping and packaging. Small steps collectively lead to big change. This is the first installation in a three-part series on sustainable practices developed on the Bio-Rad campus.

The post Green Thinking Sparks New Ideas, Better Programs: Sustainability Part One first appeared on Bio-Radiations.]]> Bio-Plex® System in Korea — Seeking to Improve Outcomes for Cancer Patients https://www.bioradiations.com/bio-plex-system-in-korea-seeking-to-improve-outcomes-for-cancer-patients/ Thu, 08 Mar 2012 19:21:05 +0000 http://www.bioradiations.com/?p=3386 Dr Hunseok Lee has known since childhood that he wanted to grow up to become a scientist. “You get to wear white gowns, look through a microscope at various substances so small they can’t be seen by the naked eye — those things were very attractive to me when I was young,” says Lee. But the realities of putting research into practice and translating ideas into experiments have proven far more strenuous than the young Lee anticipated.

The post Bio-Plex® System in Korea — Seeking to Improve Outcomes for Cancer Patients first appeared on Bio-Radiations.]]> New Multiplex Assays for Th17 Cytokine Research https://www.bioradiations.com/new-multiplex-assays-for-th17-cytokine-research/ Tue, 06 Mar 2012 20:21:16 +0000 http://www.bioradiations.com/?p=4186 The Bio-Plex Pro human Th17 cytokine panel is a unique blend of magnetic bead-based multiplex immunoassays for the robust and reproducible measurement of 15+1 soluble proteins involved in the T-helper cell type 17 immune response pathway.

The post New Multiplex Assays for Th17 Cytokine Research first appeared on Bio-Radiations.]]> Mini-PROTEAN® TGX™ Gels — Customer Testimonials https://www.bioradiations.com/mini-protean-tgx-gels-customer-testimonials/ Tue, 06 Mar 2012 20:00:15 +0000 http://www.bioradiations.com/?p=2260 Scientists from UCSD, UCD, The Salk Institute, and the University of Alabama talk about the speed, performance, and quality of electrophoresis runs using the Mini-PROTEAN TGX gels in their labs.

The post Mini-PROTEAN® TGX™ Gels — Customer Testimonials first appeared on Bio-Radiations.]]> PrimePCR™ Lookup Tool https://www.bioradiations.com/primepcr-lookup-tool/ Fri, 02 Mar 2012 23:31:29 +0000 http://www.bioradiations.com/?p=5763 Use the PrimePCR lookup tool to find assays and panels for your genes of interest.

The post PrimePCR™ Lookup Tool first appeared on Bio-Radiations.]]> A New Paradigm for Precise Quantitation of RNA https://www.bioradiations.com/a-new-paradigm-for-precise-quantitation-of-rna/ Mon, 27 Feb 2012 20:31:17 +0000 http://www.bioradiations.com/?p=4200 The one-step RT-ddPCR kit for probes creates a new paradigm for the precise quantitation of RNA by combining reverse transcription with Droplet Digital™ PCR (ddPCR™), providing an absolute measure of target RNA molecules with unrivaled precision and sensitivity

The post A New Paradigm for Precise Quantitation of RNA first appeared on Bio-Radiations.]]> Introducing iTaq Universal Supermixes for Highest Quality qPCR Results on Any Instrument https://www.bioradiations.com/introducing-itaq-universal-supermixes-for-highest-quality-qpcr-results-on-any-instrument/ Fri, 24 Feb 2012 18:21:47 +0000 http://www.bioradiations.com/?p=6311 The iTaq™ universal SYBR® Green supermix and iTaq™ universal probes supermix are ready-to-use 2x supermixes formulated for sensitive, efficient, and reproducible qPCR data on any instrument.

The post Introducing iTaq Universal Supermixes for Highest Quality qPCR Results on Any Instrument first appeared on Bio-Radiations.]]> Bio-Rad Automated Supply Center in Action https://www.bioradiations.com/bio-rad-automated-supply-center-in-action/ Fri, 10 Feb 2012 20:00:09 +0000 http://www.bioradiations.com/?p=2262 Automated supply centers reduce paper and packaging waste, apart from providing convenience. As part of its sustainability efforts, Bio-Rad is increasing the number of its supply center sites. The video describes how the supply center works.

The post Bio-Rad Automated Supply Center in Action first appeared on Bio-Radiations.]]> Third Generation PCR https://www.bioradiations.com/third-generation-pcr/ Thu, 09 Feb 2012 20:00:28 +0000 http://www.bioradiations.com/?p=4273 The McCarroll laboratory studies the biological effects of human genome polymorphism, seeking to define how genome variation influences gene expression and risk of disease.

The post Third Generation PCR first appeared on Bio-Radiations.]]> Around the World with the ChemiDoc™ MP Imaging System https://www.bioradiations.com/around-the-world-with-the-chemidoc-mp-imaging-system/ Wed, 08 Feb 2012 18:55:00 +0000 http://www.bioradiations.com/?p=4362 Bio-Rad has long demonstrated innovation in imaging, with the introduction of the stain-free gel electrophoresis workflow, which was rapidly followed by the launch of the Gel Doc™ EZ imager. Now, this progression continues with the release of the ChemiDoc™ MP imaging system.

The post Around the World with the ChemiDoc™ MP Imaging System first appeared on Bio-Radiations.]]> Application of Hexapeptide Libraries for Enhanced Protein Detection in Human Cellular Lysates https://www.bioradiations.com/application-of-hexapeptide-libraries-for-enhanced-protein-detection-in-human-cellular-lysates/ Mon, 30 Jan 2012 21:24:47 +0000 http://www.bioradiations.com/?p=2775 Hexapeptide libraries, such as Bio-Rad's ProteoMiner™ protein enrichment technology, can significantly increase sensitivity of shotgun proteomics analyses of diverse complex samples. Underlying this increased sensitivity is the partial removal of high-abundance proteins leading to an increased representation of low-abundance proteins — a phenomenon described as dynamic range compression. Currently, hexapeptide libraries have been mostly used to increase proteomic detection in fluid samples (eg: saliva, plasma, etc.), but not in cellular lysates.

The post Application of Hexapeptide Libraries for Enhanced Protein Detection in Human Cellular Lysates first appeared on Bio-Radiations.]]> Transitioning from Chemiluminescent to Multiplex Fluorescent Blotting: Things to Consider https://www.bioradiations.com/transitioning-from-chemiluminescent-to-multiplex-fluorescent-blotting-things-to-consider/ Thu, 26 Jan 2012 22:12:02 +0000 http://www.bioradiations.com/?p=1366 Today many researchers are considering changing their western blot detection method from chemiluminescence to multiplex fluorescence. There are several drivers behind this trend. Most significantly, fluorescent detection allows users to multiplex their western blots, enabling simultaneous detection of several target proteins at once, reducing or eliminating the need to strip and re-probe. Other benefits of fluorescence include better dynamic range, more quantitative results, and better signal stability over time.

The post Transitioning from Chemiluminescent to Multiplex Fluorescent Blotting: Things to Consider first appeared on Bio-Radiations.]]> Highly Efficient Lipoparticle Capture and SPR Binding Kinetics of a Membrane Protein Using the ProteOn™ XPR36 Protein Interaction Array System https://www.bioradiations.com/highly-efficient-lipoparticle-capture-and-spr-binding-kinetics-of-a-membrane-protein-using-the-proteon-xpr36-protein-interaction-array-system/ Tue, 24 Jan 2012 19:12:28 +0000 http://www.bioradiations.com/?p=1973 The genes coding for membrane proteins, such as G-protein coupled receptors (GPCR), ion channels, and other membrane-bound enzymes make up 25–30% of the human genome and membrane proteins are estimated to represent 30–40% of the top drug targets. There is growing research, industrial, and commercial interest in the study of membrane proteins in general, and more specifically in immobilizing membrane proteins to various biosensor surfaces.

The post Highly Efficient Lipoparticle Capture and SPR Binding Kinetics of a Membrane Protein Using the ProteOn™ XPR36 Protein Interaction Array System first appeared on Bio-Radiations.]]> A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data https://www.bioradiations.com/a-miqe-case-study-effect-of-rna-sample-quality-and-reference-gene-stability-on-gene-expression-data/ Thu, 15 Dec 2011 21:23:32 +0000 http://www.bioradiations.com/?p=2840 This tech report shows how qPCR can lead to erroneous conclusions regarding differences in MCM7 gene expression between normal and tumor human breast cancer samples if the key steps set out in the MIQE guidelines are not followed.

The post A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data first appeared on Bio-Radiations.]]> PROTEAN® i12™ IEF System Introduction https://www.bioradiations.com/protean-i12-ief-system-introduction/ Mon, 12 Dec 2011 20:00:30 +0000 http://www.bioradiations.com/?p=2265 Bio-Rad’s PROTEAN i12 IEF system is specially designed for running different sample types, different gradients, and multiple 2-D electrophoresis protocols at the same time. Watch the video to learn about all the new features of this IEF system.

The post PROTEAN® i12™ IEF System Introduction first appeared on Bio-Radiations.]]> QX100™ Droplet Digital™ PCR system https://www.bioradiations.com/qx100-droplet-digital-pcr-system/ Wed, 23 Nov 2011 20:42:43 +0000 http://www.bioradiations.com/?p=4212 Bio-Rad’s QX100™ Droplet Digital™ PCR system provides an absolute measure of target DNA molecules with unrivaled precision and accuracy.

The post QX100™ Droplet Digital™ PCR system first appeared on Bio-Radiations.]]> Bio-Rad Introduces New Bio-Plex® Human Cancer Assays https://www.bioradiations.com/bio-rad-introduces-new-bio-plex-human-cancer-assays/ Mon, 21 Nov 2011 21:13:37 +0000 http://www.bioradiations.com/?p=4222 Bio-Plex Pro™ Human Cancer Biomarker Panel 1 is a unique blend of magnetic bead-based assays designed to meet the needs of the most discerning preclinical and clinical researchers.

The post Bio-Rad Introduces New Bio-Plex® Human Cancer Assays first appeared on Bio-Radiations.]]> Using the new PROTEAN® i12™ IEF System to Achieve Highly Reproducible Data and Extended Separation Over Multiple pH Ranges in a Single Run https://www.bioradiations.com/using-the-new-protean-i12-ief-system-to-achieve-highly-reproducible-data-and-extended-separation-over-multiple-ph-ranges-in-a-single-run/ Mon, 21 Nov 2011 20:29:35 +0000 http://www.bioradiations.com/?p=3492 2-D electrophoresis is a multistep technique that separates proteins first by a protein’s isoelectric point and then by its molecular weight. This powerful technique can separate thousands of proteins in a complex proteome and is useful for applications such as protein profiling and biomarker discovery. The drawback of the technique is that it requires multiple steps, each of which can be time-consuming and can contribute to problems with reproducibility.

The post Using the new PROTEAN® i12™ IEF System to Achieve Highly Reproducible Data and Extended Separation Over Multiple pH Ranges in a Single Run first appeared on Bio-Radiations.]]> Use of the PROTEAN® i12™ IEF System for In-Gel Peptide Fractionation Prior to LC-MS and Comparison with Off-Gel Fractionation https://www.bioradiations.com/use-of-the-protean-i12-ief-system-for-in-gel-peptide-fractionation-prior-to-lc-ms-and-comparison-with-off-gel-fractionation/ Fri, 18 Nov 2011 21:21:16 +0000 http://www.bioradiations.com/?p=2871 This tech report compares in-gel and off-gel separation of proteins in terms of overall speed and simplicity of the workflow, resolution of the separation, and depth of proteome coverage provided. Results found that as well as higher peptide yields, in-gel separations were better suited to take full advantage of downstream analysis methods.

The post Use of the PROTEAN® i12™ IEF System for In-Gel Peptide Fractionation Prior to LC-MS and Comparison with Off-Gel Fractionation first appeared on Bio-Radiations.]]> TGX Stain-Free™ Precast Gels https://www.bioradiations.com/tgx-stain-free-precast-gels/ Wed, 16 Nov 2011 16:52:26 +0000 http://www.bioradiations.com/?p=4302 Mini-PROTEAN® TGX Stain-Free™ and Criterion™ TGX Stain-Free™ precast gels, when combined with Bio-Rad's ChemiDoc™ MP or Gel Doc™ EZ imagers, provide researchers with the fastest protein separation and visualization available using the standard Laemmli system.

The post TGX Stain-Free™ Precast Gels first appeared on Bio-Radiations.]]> Protein Expression and Purification Series — A Laboratory Course for Training Researchers https://www.bioradiations.com/protein-expression-and-purification-series-a-laboratory-course-for-training-researchers/ Tue, 15 Nov 2011 17:41:05 +0000 http://www.bioradiations.com/?p=4315 Protein Expression and Purification Laboratory Course for Training Researchers on Protein Expression and Purification.

The post Protein Expression and Purification Series — A Laboratory Course for Training Researchers first appeared on Bio-Radiations.]]> ProteOn™ HTE Sensor Chip https://www.bioradiations.com/proteon-hte-sensor-chip/ Mon, 14 Nov 2011 17:54:12 +0000 http://www.bioradiations.com/?p=4326 The HTE sensor chip is designed for label-free biomolecular interaction analysis with polyhistidine-tagged proteins. It features a novel tris-NTA (3-NTA) surface for excellent performance of polyhistidine-tagged protein capture, providing high binding stability and regeneracy. It is an ideal choice for the applications of protein-small molecule and other protein interactions requiring high sensitivity.

The post ProteOn™ HTE Sensor Chip first appeared on Bio-Radiations.]]> Stellan Hjertén Wins the California Separations Science Society Award https://www.bioradiations.com/stellan-hjerten-wins-the-california-separations-science-society-award/ Fri, 21 Oct 2011 23:52:25 +0000 http://www.bioradiations.com/?p=6345 This year the California Separation Science Society will present its annual Scientific Achievement Award to Professor Stellan Hjertén of Uppsala University. The award dinner will be held November 3, 2011 at the Waterfront Hotel in Jack London Square.

The post Stellan Hjertén Wins the California Separations Science Society Award first appeared on Bio-Radiations.]]> One Array, 36 Unique Protein Interactions https://www.bioradiations.com/one-array-36-unique-protein-interactions/ Fri, 14 Oct 2011 18:50:10 +0000 http://www.bioradiations.com/?p=4392 The ProteOn XPR36 protein interaction analysis system was introduced in 2006 to provide a novel array technology to researchers conducting label-free analysis of proteins. The ProteOn system is a high-throughput surface plasmon resonance-based screening tool for a wide range of applications from small-molecule drug discovery to antibody kinetic ranking, epitope binning, and epitope mapping.

The post One Array, 36 Unique Protein Interactions first appeared on Bio-Radiations.]]> ChemiDoc™ MP Imaging System https://www.bioradiations.com/chemidoc-mp-imaging-system/ Wed, 05 Oct 2011 21:23:44 +0000 http://www.bioradiations.com/?p=6325 The ChemiDoc MP imaging system enables stain-free operation and lets you visualize proteins at every stage of your experiment. Its flexibility and sensitivity are complemented by a simple, intuitive operation that integrates seamlessly into your workflow.

The post ChemiDoc™ MP Imaging System first appeared on Bio-Radiations.]]> PROTEAN® i12™ IEF System https://www.bioradiations.com/protean-i12-ief-system/ Tue, 04 Oct 2011 17:09:51 +0000 http://www.bioradiations.com/?p=4337 Introducing Bio-Rad's PROTEAN i12 IEF system. Each channel in the i12™ focusing tray is powered by its own power supply, enabling precise control over each IPG strip. This makes it possible to run different sample types, different gradients, and multiple protocols all at the same time.

The post PROTEAN® i12™ IEF System first appeared on Bio-Radiations.]]> PROTEAN® i12™ IEF System: Independent Voltage and Current Control Enables Optimization of First-Dimension IEF Conditions https://www.bioradiations.com/protean-i12-ief-system-independent-voltage-and-current-control-enables-optimization-of-first-dimension-ief-conditions/ Mon, 03 Oct 2011 20:18:56 +0000 http://www.bioradiations.com/?p=3101 This tech report demonstrates the advantages of independently controlled voltage and current parameters for each focusing tray in optimizing IEF conditions for a complex, insoluble membrane protein fraction.

The post PROTEAN® i12™ IEF System: Independent Voltage and Current Control Enables Optimization of First-Dimension IEF Conditions first appeared on Bio-Radiations.]]> Immun-Blot® LF (Low Fluorescence) PVDF Membranes https://www.bioradiations.com/immun-blot-lf-low-fluorescence-pvdf-membranes/ Mon, 03 Oct 2011 17:27:05 +0000 http://www.bioradiations.com/?p=4351 Immun-Blot LF (low fluorescence) PVDF membranes are protein blotting membranes optimized for fluorescent and multiplex fluorescent applications. They offer high signal-to-background ratio, low autofluorescence, and superior protein retention to maximize blot detection sensitivity and enable downstream quantitation.

The post Immun-Blot® LF (Low Fluorescence) PVDF Membranes first appeared on Bio-Radiations.]]> Accurate Counting of Bio-Plex® Magnetic or Polystyrene Beads Using the TC10™ Automated Cell Counter https://www.bioradiations.com/accurate-counting-of-bio-plex-magnetic-or-polystyrene-beads-using-the-tc10-automated-cell-counter/ Wed, 28 Sep 2011 20:15:04 +0000 http://www.bioradiations.com/?p=3158 This report demonstrates that the TC10 automated cell counter is a suitable alternative to the Coulter Counter and hemocytometer for the preparation of custom Bio-Plex assays.

The post Accurate Counting of Bio-Plex® Magnetic or Polystyrene Beads Using the TC10™ Automated Cell Counter first appeared on Bio-Radiations.]]> Bio-Plex® System Bench to Bedside https://www.bioradiations.com/bio-plex-system-bench-to-bedside/ Tue, 30 Aug 2011 19:00:21 +0000 http://www.bioradiations.com/?p=2267 Watch how the Bio-Plex® suspension array system has contributed to research findings in Dr Francis Spinale and Dr Steven Kornblau's laboratories that have the potential to impact screening and treatment of disease.

The post Bio-Plex® System Bench to Bedside first appeared on Bio-Radiations.]]> Gel Doc™ EZ Imaging System Customer Testimonial https://www.bioradiations.com/gel-doc-ez-imaging-system-customer-testimonial/ Mon, 29 Aug 2011 19:00:39 +0000 http://www.bioradiations.com/?p=2269 Harvard Medical School Postdoctoral student Sririam Kosuri explains how the Gel Doc EZ system has changed the way researchers image gels in the George Church lab at Harvard Medical School’s Wyss Institute.

The post Gel Doc™ EZ Imaging System Customer Testimonial first appeared on Bio-Radiations.]]> Gel Doc™ EZ Testimonial — UCSD https://www.bioradiations.com/gel-doc-ez-testimonial-ucsd/ Sun, 28 Aug 2011 19:00:28 +0000 http://www.bioradiations.com/?p=2271 Assistant Project Scientist Dr. Dorota Krawczyk, PhD, at the Geoff Rosenfeld Laboratory at the University of California, San Diego, describes how easy it is to use the Gel Doc EZ system.

The post Gel Doc™ EZ Testimonial — UCSD first appeared on Bio-Radiations.]]> Innovating Electrophoresis with Precast Gels https://www.bioradiations.com/innovating-electrophoresis-with-precast-gels/ Fri, 26 Aug 2011 19:00:59 +0000 http://www.bioradiations.com/?p=2273 Setting new standards for consistency and speed in the electrophoresis workflow.

The post Innovating Electrophoresis with Precast Gels first appeared on Bio-Radiations.]]> Trans-Blot Turbo Instructional Video https://www.bioradiations.com/trans-blot-turbo-instructional-video/ Wed, 24 Aug 2011 19:00:02 +0000 http://www.bioradiations.com/?p=2275 Instructional video to guide you through the features, benefits, and operation of the Trans-Blot Turbo system.

The post Trans-Blot Turbo Instructional Video first appeared on Bio-Radiations.]]> Trans-Blot® Turbo™ System vs. the iBlot System https://www.bioradiations.com/trans-blot-turbo-system-vs-the-iblot-system/ Tue, 23 Aug 2011 19:00:44 +0000 http://www.bioradiations.com/?p=2277 Battle of the blotters: see how the Trans-Blot Turbo system performs compared to the iBlot dry blotting system.

The post Trans-Blot® Turbo™ System vs. the iBlot System first appeared on Bio-Radiations.]]> Trans-Blot® Turbo™ System vs. Traditional Blotting Techniques https://www.bioradiations.com/trans-blot-turbo-system-vs-traditional-blotting-techniques/ Mon, 22 Aug 2011 19:00:37 +0000 http://www.bioradiations.com/?p=2279 See how the Trans-Blot Turbo system measures up against traditional blotting techniques.

The post Trans-Blot® Turbo™ System vs. Traditional Blotting Techniques first appeared on Bio-Radiations.]]> Identifying Cardiac Defect–Related Protein Signatures https://www.bioradiations.com/identifying-cardiac-defect-related-protein-signatures/ Mon, 15 Aug 2011 19:09:40 +0000 http://www.bioradiations.com/?p=3401 Identifying Cardiac Defect–Related Protein SignaturesAugust 15, 2011
"The first opportunity I had to lay my hand on a human heart I knew that was that — I knew that I wanted to know how the heart works, but more importantly, how it doesn't work," says Dr Francis Spinale, describing his inspiration to both research and treat cardiac disease. Using the Bio-Plex® suspension array system, researchers in his laboratory are discovering protein signatures that have the potential to inform decision strategies in cardiac patient treatment.

The post Identifying Cardiac Defect–Related Protein Signatures first appeared on Bio-Radiations.]]> Chemokines and Cytokines as Potential Markers for Personalizing Leukemia Treatment https://www.bioradiations.com/chemokines-and-cytokines-as-potential-markers-for-personalizing-leukemia-treatment/ Wed, 10 Aug 2011 19:00:30 +0000 http://www.bioradiations.com/?p=3305 Dr. Steven Kornblau has been on faculty at MD Anderson Cancer Center in Houston, Texas, for 20 years, where he divides his time between research into further understanding leukemia disease states, and a clinical practice where he treats a small number of patients with the disease. Recently, his laboratory used the Bio-Plex® suspension array system to discover how cytokine and chemokine profiles alone can be prognostic for patients with the disease, and therefore have to potential to assist development of personalized treatments.

The post Chemokines and Cytokines as Potential Markers for Personalizing Leukemia Treatment first appeared on Bio-Radiations.]]> High-Throughput Automated Extraction of RNA Using the Aurum™ Total RNA 96 Kit https://www.bioradiations.com/high-throughput-automated-extraction-of-rna-using-the-aurum-total-rna-96-kit/ Thu, 16 Jun 2011 20:22:34 +0000 http://www.bioradiations.com/?p=2931 This tech report describes an automated method of RNA extraction using the Aurum total RNA 96 kit and its use in a magnetic–bead based automated process. Results show that throughput can be easily increased with the Aurum total RNA 96 kit and that the automation protocols yield more reliable results, particularly when high sample volumes are used.

The post High-Throughput Automated Extraction of RNA Using the Aurum™ Total RNA 96 Kit first appeared on Bio-Radiations.]]> C-96 Autosampler for Reliable Protein Purification Sample Application https://www.bioradiations.com/c-96-autosampler-for-reliable-protein-purification-sample-application/ Wed, 15 Jun 2011 19:00:34 +0000 http://www.bioradiations.com/?p=3296 The C-96 autosampler improves the efficiency of protein purification workflows by enabling automated sample application. The straightforward tray designs are easy to use; the standard tray accommodates 84 sample vials (5 ml each) plus three additional vials (10 ml each) for mixing and dilution protocols.

The post C-96 Autosampler for Reliable Protein Purification Sample Application first appeared on Bio-Radiations.]]> Targeting Suppression of B-Cell Lymphoma Proliferation https://www.bioradiations.com/targeting-suppression-of-b-cell-lymphoma-proliferation/ Wed, 15 Jun 2011 19:00:29 +0000 http://www.bioradiations.com/?p=2579 Rossi considers the discovery of RNAi as pivotal to antiviral discovery. "RNAi has become another important, powerful genetic tool we can use to further our antiviral work," he says. Rossi's lab became the first to publish research demonstrating the expression of small interfering RNAs (siRNAs) to inhibit HIV replication in human cells. Various aspects of RNAi and siRNAs continue to play a key role in his research on viral diseases and lymphomas.

The post Targeting Suppression of B-Cell Lymphoma Proliferation first appeared on Bio-Radiations.]]> Hard-Shell® 384-Well 480 PCR Plates https://www.bioradiations.com/hard-shell-384-well-480-pcr-plates/ Tue, 14 Jun 2011 19:00:35 +0000 http://www.bioradiations.com/?p=3248 The proprietary warp-free Hard-Shell plates have a reputation as premium plates and were widely used on Bio-Rad, MJ, and Applied Biosystems instruments for the Human Genome Project. Now, researchers can use the same high-quality Hard-Shell plates on Roche LightCycler 480 instruments.

The post Hard-Shell® 384-Well 480 PCR Plates first appeared on Bio-Radiations.]]> Using Precision Plus Protein™ WesternC™ Standards to Validate Major Steps in the Western Blotting Workflow https://www.bioradiations.com/using-precision-plus-protein-westernc-standards-to-validate-major-steps-in-the-western-blotting-workflow/ Mon, 13 Jun 2011 19:00:59 +0000 http://www.bioradiations.com/?p=3224 Western blotting, although a commonly used technique, involves a time-consuming multi step process. The whole western blotting workflow could take up to three days to complete. Thus, having a tool to monitor each of the major steps in the western blotting workflow reduces chances of finding out at the very end of the workflow that an experiment was not successful.

The post Using Precision Plus Protein™ WesternC™ Standards to Validate Major Steps in the Western Blotting Workflow first appeared on Bio-Radiations.]]> Bio-Plex® Assay Configurations Simplified with the Bio-Plex Assay Builder for Complete Flexibility https://www.bioradiations.com/bio-plex-assay-configurations-simplified-with-the-bio-plex-assay-builder-for-complete-flexibility/ Mon, 13 Jun 2011 19:00:55 +0000 http://www.bioradiations.com/?p=2945 Now you can optimize research expenditure by multiplexing only the targets you need to get the answers you're seeking. This newly updated tool allows you to choose from within panels to build the perfect kit.

The post Bio-Plex® Assay Configurations Simplified with the Bio-Plex Assay Builder for Complete Flexibility first appeared on Bio-Radiations.]]> Gel Doc™ EZ Imaging System – A Tale of Speed and Simplicity in Two Cities https://www.bioradiations.com/gel-doc-ez-imaging-system-a-tale-of-speed-and-simplicity-in-two-cities/ Mon, 13 Jun 2011 19:00:54 +0000 http://www.bioradiations.com/?p=2591 The Gel Doc™ EZ imaging system makes an impact from coast to coast — at the Wyss Institute for Biologically Inspired Engineering at Harvard University in Boston, and in the Geoff Rosenfeld Laboratory at the University of California, San Diego.

The post Gel Doc™ EZ Imaging System – A Tale of Speed and Simplicity in Two Cities first appeared on Bio-Radiations.]]> Simplicity in Imaging: From Conception to Launch https://www.bioradiations.com/simplicity-in-imaging-from-conception-to-launch/ Mon, 13 Jun 2011 19:00:41 +0000 http://www.bioradiations.com/?p=3038 When it comes to gel imaging and blotting, conventional imaging devices have tended to offer either high-performance, complex functionality or low-cost, low-performance simplicity. That is, until now.

The post Simplicity in Imaging: From Conception to Launch first appeared on Bio-Radiations.]]> Guidelines for Developing Robust and Reproducible High-Resolution Melt Analysis Assays https://www.bioradiations.com/guidelines-for-developing-high-resolution-melt-analysis/ Mon, 13 Jun 2011 19:00:40 +0000 http://www.bioradiations.com/?p=1312 Classifying and understanding genetic variation between populations and individuals is an important aim in the field of genomics. High resolution melt (HRM) analysis is the quantitative analysis of the melt curve of a DNA fragment following amplification by PCR and can be considered the next-generation application of amplicon melting analysis. Careful sample preparation and planning of experimental and assay design are crucial for robust and reproducible results.

The post Guidelines for Developing Robust and Reproducible High-Resolution Melt Analysis Assays first appeared on Bio-Radiations.]]> Efficient Electrophoresis: Aiding Advances in Cardiomiopathy Research https://www.bioradiations.com/efficient-electrophoresis-aiding-advances-in-cardiomiopathy-research/ Mon, 13 Jun 2011 19:00:30 +0000 http://www.bioradiations.com/?p=2964 The electrophoretic workflow has undergone few changes since it was solidified as a fundamental tool in life science research in the 1970s. Today, just like then, researchers need to prepare samples, load gels, then perform separation and further downstream analysis, including western blotting.

The post Efficient Electrophoresis: Aiding Advances in Cardiomiopathy Research first appeared on Bio-Radiations.]]> Unlocking the Potential of Stem Cell Research Using the EpiQ™ Chromatin Analysis Kit https://www.bioradiations.com/unlocking-the-potential-of-stem-cell-research-using-the-epiq-chromatin-analysis-kit/ Mon, 13 Jun 2011 19:00:05 +0000 http://www.bioradiations.com/?p=2603 Bedtime stories in Dr David Schaffer's — one of today's pioneers in stem cell research — childhood home were often not standard fairy tales. With both parents in careers as biomedical researchers (his mother in drug development and clinical trials for a major pharmaceutical company and his father, in cardiovascular research and a pharmacology professor) much of the conversation as far back as Schaffer can remember centered on biology and science.

The post Unlocking the Potential of Stem Cell Research Using the EpiQ™ Chromatin Analysis Kit first appeared on Bio-Radiations.]]> Protein Quantitation Assays — Frequently Asked Questions https://www.bioradiations.com/protein-quantitation-assays-frequently-asked-questions/ Mon, 13 Jun 2011 18:23:22 +0000 http://www.bioradiations.com/?p=1652 Protein quantitation is an important part of many workflows. The estimation or quantitation of the protein in a sample frequently allows researchers to understand the results from their work and make decisions on the subsequent steps. Presented are answers to some of the most frequently ask questions related to usage of Bio-Rad's colorimetric assays for protein quantitation.

The post Protein Quantitation Assays — Frequently Asked Questions first appeared on Bio-Radiations.]]> Using the Precision Plus Protein™ WesternC™ Standard as a QC Tool at Key Steps in Your Electrophoresis and Blotting Workflow https://www.bioradiations.com/using-the-precision-plus-protein-westernc-standard-as-a-qc-tool-at-key-steps-in-your-electrophoresis-and-blotting-workflow/ Mon, 13 Jun 2011 16:16:18 +0000 http://www.bioradiations.com/?p=3181 As a fundamental tool in protein research, it is critical that all steps of the electrophoresis workflow (from running gels to visualizing blots) are performed successfully to ensure accurate, reliable, and reproducible results. Protein standards are often used to monitor electrophoretic separation and transfer efficiency but do not always enable ladder visualization after blot development.

The post Using the Precision Plus Protein™ WesternC™ Standard as a QC Tool at Key Steps in Your Electrophoresis and Blotting Workflow first appeared on Bio-Radiations.]]> ProteOn™ XPR36 Protein Interaction Array System https://www.bioradiations.com/proteon-xpr36-protein-interaction-array-system/ Sat, 11 Jun 2011 19:00:59 +0000 http://www.bioradiations.com/?p=2283 An overview of how the ProteOn XPR36 protein interaction array system can benefit your surface-plasmon reasonance­-based research.

The post ProteOn™ XPR36 Protein Interaction Array System first appeared on Bio-Radiations.]]> Nuvia™ S Media — A High-Capacity Cation Exchanger for Biomolecule Purification and Downstream Processing https://www.bioradiations.com/nuvia-s-media-a-high-capacity-cation-exchanger-for-biomolecule-purification-and-downstream-processing/ Thu, 09 Jun 2011 19:00:58 +0000 http://www.bioradiations.com/?p=2926 Nuvia S media is a high-capacity strong cation exchanger (CEX) developed to operate over a range of experimental conditions, allowing scientists to scale up from laboratory- to process-scale more quickly and easily. Our data demonstrate that the high capacity and superb purification of Nuvia S media can significantly improve productivity by offering the robustness, capacity, and speed for processing large load volumes with smaller columns and less buffer consumption.

The post Nuvia™ S Media — A High-Capacity Cation Exchanger for Biomolecule Purification and Downstream Processing first appeared on Bio-Radiations.]]> All cDNA Synthesis Kits Are Not Created Equal: RNase A Inhibition and Its Effect on qPCR Data https://www.bioradiations.com/all-cdna-synthesis-kits-are-not-created-equal-rnase-a-inhibition-and-its-effect-on-qpcr-data/ Thu, 09 Jun 2011 19:00:40 +0000 http://www.bioradiations.com/?p=2891 Not all cDNA synthesis kits have the same capacity to protect the integrity of RNA. An optimal level of RNase inhibitor is critical for powerful RNase inhibition and efficient reverse transcription. The iScript™ cDNA synthesis kit comes with a 5x iScript reaction mix and iScript reverse transcriptase, which is also blended with a potent RNase inhibitor.

The post All cDNA Synthesis Kits Are Not Created Equal: RNase A Inhibition and Its Effect on qPCR Data first appeared on Bio-Radiations.]]> Comparison between the TC10™ Automated Cell Counter and the Lactate Dehydrogenase (LDH) Assay to Assess Cellular Toxicity in vitro https://www.bioradiations.com/comparison-between-the-tc10-automated-cell-counter-and-the-lactate-dehydrogenase-ldh-assay-to-assess-cellular-toxicity-in-vitro/ Thu, 09 Jun 2011 19:00:24 +0000 http://www.bioradiations.com/?p=2938 This tech report presents the results of a comparative study between the TC10 automated cell counter and the classical LDH assay to measure cell viability in suspension cells. Results show that the TC10 cell counter can replace the LDH assay to measure both the viability of cell suspensions and the cytotoxic effects of various compounds.

The post Comparison between the TC10™ Automated Cell Counter and the Lactate Dehydrogenase (LDH) Assay to Assess Cellular Toxicity in vitro first appeared on Bio-Radiations.]]> Using the TC10™ Automated Cell Counter https://www.bioradiations.com/using-the-tc10-automated-cell-counter/ Thu, 09 Jun 2011 19:00:22 +0000 http://www.bioradiations.com/?p=2281 A better, more accurate, easier way to count cells than a traditional hemocytometer — learn how this automated system works.

The post Using the TC10™ Automated Cell Counter first appeared on Bio-Radiations.]]> Bio-Plex Pro™ Mouse Th17 Cytokines https://www.bioradiations.com/bio-plex-pro-mouse-th17-cytokines/ Thu, 09 Jun 2011 19:00:02 +0000 http://www.bioradiations.com/?p=2874 Bio-Plex Pro™ mouse Th17 assays are magnetic bead–based multiplex assays that offer accurate and reproducible measurements of 20 target molecules in serum, plasma, and tissue culture sample types for mouse models. These assays have been developed to provide reliable performance with the flexibility required to meet all of your research needs.

The post Bio-Plex Pro™ Mouse Th17 Cytokines first appeared on Bio-Radiations.]]> Research Applications for the EpiQ™ Chromatin Analysis Kit https://www.bioradiations.com/research-applications-for-the-epiq-chromatin-analysis-kit/ Wed, 08 Jun 2011 19:00:31 +0000 http://www.bioradiations.com/?p=2845 EpiQ chromatin analysis kit is a novel epigenetics research tool for the quantitative assessment of chromatin structure in cultured cells. By combining in situ chromatin digestion, genomic DNA purification, and real-time PCR, the chromatin state for several gene promoters can be studied simultaneously.

The post Research Applications for the EpiQ™ Chromatin Analysis Kit first appeared on Bio-Radiations.]]> The T100™ Thermal Cycler https://www.bioradiations.com/the-t100-thermal-cycler/ Wed, 01 Jun 2011 19:00:52 +0000 http://www.bioradiations.com/?p=2288 Why the T100 thermal cycler is the smart choice for your PCR.

The post The T100™ Thermal Cycler first appeared on Bio-Radiations.]]> Trans-Blot® Turbo™ System https://www.bioradiations.com/trans-blot-turbo-system/ Wed, 01 Jun 2011 19:00:46 +0000 http://www.bioradiations.com/?p=2290 Step-by-step with the Trans-Blot Turbo system — from building the blot sandwich to programming the run.

The post Trans-Blot® Turbo™ System first appeared on Bio-Radiations.]]> 1000-Series Touch Thermal Cyclers https://www.bioradiations.com/1000-series-touch-thermal-cyclers/ Wed, 01 Jun 2011 19:00:00 +0000 http://www.bioradiations.com/?p=2286 Touch the future of PCR; introducing the 1000-series Touch thermal cyclers.

The post 1000-Series Touch Thermal Cyclers first appeared on Bio-Radiations.]]> One of the Best Places to Work in 2011 https://www.bioradiations.com/one-of-the-best-places-to-work-in-2011/ Tue, 31 May 2011 19:00:38 +0000 http://www.bioradiations.com/?p=2292 The people behind the science – the reason Bio-Rad has been named one of the "2011 Best Places to Work in the Bay Area" by the San Francisco Business Times.

The post One of the Best Places to Work in 2011 first appeared on Bio-Radiations.]]>