Tip of the Week

#7 How Do You Detect DNA Contamination in Your Protein During Purification?

Use a multiwave detector — this will add flexibility to your chromatography system, enabling the simultaneous monitoring at up to four wavelengths for the detection of proteins, peptides, nucleic acids, and chromophores such as hemoglobin in complex biological mixtures.

The ratio of average absorbance at 280 nm and 260 nm (or 255 nm) can be used to determine the purity of your protein from DNA contaminants. Some chromatography software programs will allow you to calculate this value for each fraction, allowing you to select fractions containing the least amount of impurities.

Previous post

Six Tips for Increasing the Reproducibility of qPCR Experiments

Next post

Flow Cytometry: Basic Definitions