ArticlesDroplet Digital PCR (ddPCR)Drug Discovery and Development

Precise Cell and Gene Therapy Potency Quantification

Inadequate quality control assays shouldn’t limit cell and gene therapy applications. Instead, quantifying the potency of cell and gene therapies is essential for successful therapeutics. In this article, we describe multiple leading potency assays developed using Droplet Digital PCR (ddPCR) technology, including an assay that quantifies transgene integration and expression in one step.
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ArticlesDrug Discovery and Development

Top 8 Advantages of Electroporation in Cell and Gene Therapy

Cell and gene therapies have seen record growth due to their potential to address the underlying causes of genetic and acquired diseases. Non-viral delivery methods are increasing in popularity due to their safety, cost-effectiveness, reduction in manufacturing time, and flexibility compared to viral vector delivery methods. Electroporation is a simple, broadly applicable method capable of delivering molecules to a wide range of cell types for either cell or gene therapy. This article outlines the top advantages of using electroporation in cell and gene therapy and why it might be a good approach for your own research group.
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ArticlesDrug Discovery and Development

Unlocking the Power of Analysis with Automation

Disparate systems, distributed teams, and overly complex technologies are a recipe for inefficiency in research and development (R&D) laboratories, especially in drug development. With the pressure to meet challenging deadlines and strict regulations, it is easy for working practices to become disconnected. To improve the efficiency, accuracy, and consistency of data generation, acquisition, and management, R&D organizations are looking to streamline their lab practices by investing in technologies that automate data generation, accelerate data collection speed and storage, and enhance the quality and reliability of the information produced. Lab automation presents exciting opportunities for future research, promising novel advances in machine learning and artificial intelligence (AI), liquid handling robotics, and cloud-based data workflows.
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ArticlesDroplet Digital PCR (ddPCR)Drug Discovery and Development

Monitoring CAR T-Cell Therapy Quality with Droplet Digital PCR

The CAR T-cell manufacturing process involves high potential for variability. Learn how sensitive ddPCR assays can be used to ensure safety and efficacy.
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ArticlesDroplet Digital PCR (ddPCR)Drug Discovery and Development

CDMOs Drive Cell and Gene Therapy from Behind the Scenes

As more of the cell and gene therapies revolutionizing healthcare are approved for use, innovator companies usually get the credit. But many therapies would never make it to market without the expertise and capabilities of contract development and manufacturing organizations (CDMOs) operating behind the scenes. Learn more about how CDMOs enable new cell and gene therapies to come to market, and how Droplet Digital PCR can help.
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ArticlesDrug Discovery and DevelopmentGeneral Interest

Advanced Cancer Therapeutics: Increasing Efficacy and Specificity and Reducing Toxicity

Research into human diseases has led to the development of novel monoclonal antibody drugs. These monoclonal antibody therapies have been successful because of their specificity and selectivity. However, ongoing development and successfully launching a new mAb drug require robust and reliable methods to screen for drug targets, demonstrate drug safety and efficacy, and scale up manufacturing to meet the stringent regulatory guidelines required for approval.
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Success and QC in Cell and Gene Therapy
ArticlesDroplet Digital PCR (ddPCR)Drug Discovery and Development

With Cell and Gene Therapies, Success and Quality Control Go Hand-in-Hand

While the FDA has approved Abecma, the first CAR-T cell therapy in its class, regulatory approval is just the beginning for cell and gene therapies. Such therapies’ safety and financial viability rest on a robust manufacturing and quality control program with the flexibility to scale up and out. Droplet Digital PCR is a sensitive biopharmaceutical quality control tool, offering absolute quantification of nucleic acids and other molecules in a sample.
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ArticlesCell BiologyCOVID-19Drug Discovery and DevelopmentFeatured StoriesGeneral InterestResearch Highlights

Immune Surveillance in SARS-CoV-2 Vaccine Development

The ability to characterize the target of cell-mediated immune response is crucial during vaccine development. In studying the characterization of SARS-CoV-2 infection pathogenesis in humans, a research team identified neutralizing antibodies that provided context for vaccine antigen design. Discover how the ZE5 Cell Analyzer was used to detect activation-induced markers, measure intracellular cytokines, and identify spike protein–specific antibodies in a rapid, high-throughput manner.
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ArticlesDroplet Digital PCR (ddPCR)Drug Discovery and Development

The Role of ddPCR in Developing Safe and Effective AAV-Based Gene Therapies

Developing adeno-associated virus (AAV)-mediated gene therapies requires accurate and precise quality control. Read how Droplet Digital PCR technology provides the sensitivity needed to assess the potential safety and effectiveness of these treatments.
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ArticlesBio-Plex Multiplex AssaysDrug Discovery and DevelopmentGeneral Interest

Multiplex Immunoassays in the Race for a COVID-19 Vaccine

In vaccine development, safety and efficacy are critical. Recent studies have shown that multiplex immunoassays offer a flexible way to monitor immune responses. With the ever-increasing pressure on scientists to develop a practical COVID-19 vaccine, multiplex immunoassays stand out as a proven tool.
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