Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR
One-step multiplex RT-qPCR is a technique used to rapidly quantify multiple targets directly from RNA in a single reaction. But proper optimization and validation is essential for its success. These five tips will have you designing primers and probes, selecting reporters, and validating and optimizing your experiments like a pro.
Hanging with My Chromies Podcast Series
The Hanging with My Chromies podcast series is focused on the many surprising applications of chromatography and the stories surrounding them. Join us every month to see what you can learn.
Resolved — Comic Series
Follow along as a new starry-eyed graduate student learns the ins and outs of life as a research scientist, gaining valuable western blotting tips and tricks. Along for the ride are a seasoned postdoctoral fellow and traditional principal investigator.
Expanded Droplet Digital PCR Multiplexing Capability Using Two Different Strategies
Multiplexing with Droplet Digital PCR (ddPCR) allows for improved sensitivity and precision, as well as increased low-level detection in limited sample types like liquid biopsies. Learn about amplitude and probe-mixing multiplexing and how our ddPCR Assays can help set you up for multiplexing success.
Fluorescent Immunoblots and Multiplex Analysis
Presented by: Gary F. Ross, PhD, Proteomics Field Application Scientist
Date: October 9, 2019
Time: 9 AM, U.S. Pacific time
Learn best practices for fluorescent western blotting, including multiplex analysis and image acquisition.
Purification of Antibodies with Nuvia aPrime 4A Hydrophobic Anion Exchange Resin
Our new hydrophobic anion exchange mixed-mode resin, Nuvia aPrime 4A, has been developed to overcome existing challenges in antibody purification. See how we purified an acidic and basic antibody through bind-elute and flow-through modes of purification, respectively, and helped to improve process productivity and economy.
Tyrosine Phosphorylation of EGF R
Growth factors are protein signaling molecules that play crucial roles in the regulation of numerous cellular processes such as proliferation, differentiation, and survival. Learn more about the phosphorylation of one such factor, the epidermal growth factor receptor (EGF R).
Sensitive Codetection of Viral-Pathogen RNA and DNA
Using One-Step Multiplex RT-qPCR
RT-qPCR is a well-established method for the detection, quantification, and typing of different microbial agents. However, when dealing with the challenges of pathogen detection, not all RT-qPCR reagents are created equal. See how we used a novel one-step multiplex RT-qPCR supermix to demonstrate sensitive codetection of viral RNA and DNA targets in a multiplex setting.
End the Cycle of Repeated, Failed Experiments with a Robust Field Guide to qPCR
qPCR has become ubiquitous for nucleic acid detection and quantification and remains the gold standard for a wide array of applications. To help you publish high-quality, reproducible data that reflect true experimental conditions, we’ve developed this comprehensive guide to performing the ultimate qPCR experiment.
A New AEX Mixed-Mode Chromatography Resin for High Selectivity and Recovery
Presented by: Xuemei He, PhD, R&D Manager, Chromatography Media Chemistry, Bio-Rad Laboratories
This webinar highlights the properties of Bio-Rad’s newest hydrophobic AEX chromatography resin, Nuvia aPrime 4A, which is designed to facilitate selective and reversible binding of target molecules for higher purity and recovery over traditional chromatography resins.