Electrophoresis/Western Blotting VideoTips and Techniques VideoVideos

Quick Tips for Western Blotting

Learn how to improve your western blotting results with our Quick Tips video series. Watch these 1–2 minute videos see how to perfect each step of the western blotting process.
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Videos

A Novel Technology for Post-Library rRNA Depletion

The SEQuoia RiboDepletion Kit employs novel technology to deplete ribosomal RNA while preserving rare transcripts and small RNAs, as well as minimizing the risk of degrading or low abundant samples. The SEQuoia RiboDepletion Kit also offers a streamlined workflow and multiplex compatibility, leading to significant time and cost savings compared to traditional methods. Watch this video to learn more.
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Electrophoresis/Western Blotting VideoVideos

Choosing Validated Antibodies for Trustworthy Data

The lack of reproducibility in research has been a source of frustration for scientists. Learn why antibody validation is critical for ensuring specificity and what validation methods Bio-Rad is implementing. Our scientists validate the performance of antibodies through CRISPR-Cas9 gene editing by knockout (KO), siRNA knockdown (KD), and immunoprecipitation followed by mass spectrometry (IP-MS).
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Chromatography VideoVideos

A Scalable Immobilized Metal Affinity Chromatography Media for Process Purification

Downstream process purification of proteins requires a resin with optimized bead size for ideal pressure/flow properties and decent dynamic binding capacity (DBC) that provides production efficiencies and good process economics. Nuvia IMAC Resin provides the mechanical strength, pore structures, ligand density, and particle size distribution required for an operational run at 300 cm/hr with a DBC of 40 mg/ml at 2 bar column backpressure. Protein purification can efficiently be scaled up from milligrams in the lab to kilograms in bioprocess manufacturing. The chemical stability of Nuvia IMAC Resins ensure efficient column regeneration, reproducibility between purification runs, extended column lifetime, and product purity. Watch our video to learn how Nuvia IMAC Resins overcome the challenges associated with process purification of proteins using metal affinity chromatography.
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ArticlesChromatography VideoVideos

Two-Step Purification of Low-Expressing Recombinant Exoprotein A Using Mixed-Mode Chromatography

Purifying proteins that are not highly expressed can be challenging. Watch this video to learn about a case study demonstrating an efficient workflow for two-step purification of a low-expressing recombinant protein using mixed-mode chromatography.
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ArticlesChromatography VideoTips and Techniques VideoVideos

Single-Step Column Purification for H1N1 Virus
Using Nuvia HP-Q AEX Resin

Learn how a team developed a single-step workflow for column purification of the Influenza A H1N1 subtype using the recently developed Nuvia HP-Q Anion Exchange Resin. This protocol is scalable, efficient, and produced good yields with high purity.
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Multiplex Cytokine Immunoassays
ArticlesBio-Plex Multiplex Assays VideoVideos

Advantages of Multiplexing with Bio-Plex Assays

The Bio-Plex Assay development process is designed to produce high performance multiplex assays. We prioritize sensitivity, broad dynamic range, reproducibility, and shorter incubation times to get you meaningful results faster. Watch this video to learn more about our SMARTS approach to assay development.
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ArticlesChromatography VideoCustomer Stories

Maximizing the Efficiency of Production Workflows with NGC Chromatography Systems

See how an antibody expression service provider, Evitria (Zurich, Switzerland) optimized and expanded their antibody production services using the NGC Chromatography Systems to meet the growing demands of their customers.
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Discover Our Capabilities
ArticlesCell Biology VideoChromatography VideoElectrophoresis/Western Blotting VideoReal-Time qPCR/PCR Video

Discover Our Capabilities

From flow cytometry to chromatography to western blot imaging to PCR plastics, learn more about Bio-Rad and see what’s possible in your research.
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Understanding the Long Noncoding Transcriptome
Real-Time qPCR/PCR VideoVideos

Discover lncRNA: Understanding the Long Noncoding Transcriptome

Learn about the exciting new field of long noncoding RNA (lncRNA) and how this newly discovered class of RNA compares to messenger RNA (mRNA).
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