Real-Time qPCR/PCR VideoVideos

Introducing the PTC Tempo Thermal Cycler

The PTC Tempo Thermal Cycler represents the next generation of conventional PCR thermal cyclers from Bio-Rad. Engineered to maintain thermal performance, the PTC Tempo Thermal Cycler offers the flexibility to scale with your laboratory. The design features a refreshed, intuitive user interface and flexible connectivity options for streamlining protocol management. A motorized lid enables automation, taking your PCR to the next level with modern instrument control.
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Real-Time qPCR/PCR VideoVideos

The CFX Duet Real-Time PCR System: Our Most Accessible qPCR Technology

Through the CFX Duet Real-Time PCR System, our newest generation of quantitative PCR (qPCR) instrumentation, this technology is now even more accessible for molecular biologists and other professionals doing essential qPCR. With CFX Maestro Software, you can perform duplex detection, gene expression, and genotyping, all without paying for additional optics you don’t need. Check out our video tour of the new CFX Duet System.
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Discover Our Capabilities

From flow cytometry to chromatography to western blot imaging to PCR plastics, learn more about Bio-Rad and see what’s possible in your research.
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Understanding the Long Noncoding Transcriptome
Real-Time qPCR/PCR VideoVideos

Discover lncRNA: Understanding the Long Noncoding Transcriptome

Learn about the exciting new field of long noncoding RNA (lncRNA) and how this newly discovered class of RNA compares to messenger RNA (mRNA).
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Using PrimePCR™ qPCR Assays to Understand Breast Cancer Metastasis

Traci Lyons describes how PrimePCR Assays ended her laboratory’s struggle to find a qPCR assay that could reliably detect transcription of the cyclooxygenase-2 (Cox-2) gene, which they had identified as potentially prometastatic. Using the PrimePCR Assay for Cox-2, researchers in her lab were finally able to verify cyclooxygenase-2 knockdown, which is a necessary step toward confirming its role in breast cancer metastasis.
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How to Design Hydrolysis Probes for qPCR

What’s the optimal melting temperature for a hydrolysis probe? How long should your probe be? And why should you avoid 5′ guanines like the plague? This episode answers these questions and gives you five simple tricks for designing the perfect hydrolysis probe.
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Six Tips for Increasing the Reproducibility of qPCR Experiments

Follow these six simple tips and you’ll increase the reproducibility of your qPCR experiments.
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How to Minimize Contamination in qPCR Experiments

Are you using ethanol to prep your bench for qPCR? Watch this episode to find out why that might be a bad idea. We’re sharing seven tips for minimizing contamination of qPCR experiments.
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How to Design Primers for qPCR

How long should a qPCR primer be and what types of sequences should you avoid? In this episode we share eight tips and tricks for primer design for your qPCR experiments.
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High Quality Gene Expression from Precious Samples
— A Simplified Workflow

Obtaining enough starting material to complete multiple gene expression experiments can be challenging when working with precious and limited samples. Find out how Mark Kibschull of the Lunenfeld-Tanenbaum Research Institute in Toronto determines pluripotency and differentiation of limited human embryonic stem cells using Bio-Rad’s SingleShot Cell Lysis Kit, PreAmp Supermix, and PrimePCR validated primer panels. This simplified workflow allows for the gene expression analysis of hundreds of genes from as little as 100 human embryonic stem cells, and without the cumbersome step of isolating and purifying RNA.
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