ArticlesCell BiologyDrug Discovery and Development

Developing Biotherapeutics for Lung Cancer Treatment

In recent years, biologics — drugs derived from living culture systems — have become increasingly significant in the fight against a plethora of conditions. Unfortunately, the efficiency of these drugs comes at a cost. A daily dose of a biologic drug costs, on average, 22 times more than conventional small-molecule drugs. Therefore, biosimilars are developed to make them more accessible. Lung cancer is an example where the development of biosimilars could increase treatment options available for patients.
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ArticlesCell BiologyResearch Highlights

Efficacy of a Novel Treatment for Multiple Myeloma Established by Flow Cytometry

Autologous stem cell transplantation (ASCT) is an effective treatment strategy for multiple myeloma (MM) but isolating enough hematopoietic stem and progenitor cells (HSPCs) for transplantation can be challenging. The selective CXCR4 inhibitor motixafortide has been shown to be a safe and efficacious HSPC mobilization agent in a phase 2 trial when paired with G-CSF. Crees et al. (2023) report the results of the phase 3 trial, in which they used the ZE5 Cell Analyzer to explore motixafortide’s mobilizing effects.
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ArticlesCell BiologyElectrophoresis/Western Blotting

In-Cell Western: A Versatile Tool for Quantitative Analysis of Protein Expression In-Situ

Protein expression analysis is essential in cell biology research, as it provides valuable insights into the molecular mechanisms that underlie cellular functions. Traditional methods for protein analysis, such as western blotting and ELISA, are useful for studying protein expression in cell lysates. However, these methods require labor-intensive sample preparation and do not provide information on the spatial distribution of proteins within intact cells. In-Cell Western is a powerful technique that enables quantitative and qualitative in situ protein expression analysis.
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ArticlesCell Biology

How to Build Bigger Immunophenotyping Panels with StarBright Dyes

With the advancement of technology, flow cytometers are now able to detect a much greater range of fluorescent dyes, facilitating the analysis of more extensive and complex multicolor immunophenotyping assays. But this has also introduced more pitfalls to navigate during the design of these experiments. Find out how StarBright Dyes can help circumvent many of these issues, enabling consistent and reliable results.
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ArticlesCell Biology

Flow Cytometry Empowers CAR T-Cell Therapies

Chimeric antigen receptor (CAR) T cells represent the next generation of therapeutic interventions and are a major advancement in personalized disease treatment. CAR T-cell therapies utilize a patient’s own T cells to recognize and destroy cancer cells or other disease-causing cells. Find out how the ZE5 Cell Analyzer has helped overcome some of the challenges associated with development of these powerful new therapies.
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ArticlesCell Biology

Leading the Way in Our Understanding of COVID-19: Peptide Megapool Assays

Understanding how T cells respond to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical in developing long-term therapeutic approaches, as well as helping inform strategies for future pandemics. Learn how one team at the La Jolla Institute for Immunology in San Diego, California, has been using novel T-cell activation assays to understand how our immune system responds to SARS-CoV-2.
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ArticlesCell Biology

5 Reasons to Read the Antibody Advice Guide

In the day-to-day pressures of a research environment, it is natural to want to generate important data as quickly as possible. However, there are real benefits in ensuring that the antibody you have picked is the best for your experiment and spending time aliquoting and titrating your antibodies. Bio-Rad recently launched a free Antibody Advice Guide to equip scientists with the knowledge to get the best out of their experiments with antibodies. Read on for five examples of valuable information that you will learn from the guide, helping you generate publication-ready data.
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ArticlesCell Biology

5 High-Throughput Screening Applications Using Flow Cytometry

For many scientists, high-throughput flow cytometry screens are associated with drug or compound libraries, but did you know that you can also apply this technique to study microorganisms, clinical melanoma samples, and even protein trafficking? Read on for five examples of the use of flow cytometry in diverse, high-throughput screening applications.
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ArticlesCell BiologyFeatured StoriesGeneral InterestProtocols and Tips

6 Tips to Secure Funding for Flow Cytometers

We asked core facilities staff and research scientists from the U.S. and Europe for their top tips for securing funding for large instrumentation, such as a cell analyzer or cell sorter. Here are the key questions you should consider while preparing your grant application to increase your chances of a successful outcome.
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ArticlesCell BiologyCOVID-19Drug Discovery and DevelopmentFeatured StoriesGeneral InterestResearch Highlights

Immune Surveillance in SARS-CoV-2 Vaccine Development

The ability to characterize the target of cell-mediated immune response is crucial during vaccine development. In studying the characterization of SARS-CoV-2 infection pathogenesis in humans, a research team identified neutralizing antibodies that provided context for vaccine antigen design. Discover how the ZE5 Cell Analyzer was used to detect activation-induced markers, measure intracellular cytokines, and identify spike protein–specific antibodies in a rapid, high-throughput manner.
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