Expanded Droplet Digital PCR Multiplexing Capability Using Two Different Strategies
ArticlesDroplet Digital PCR (ddPCR)Technical Reports

Expanded Droplet Digital PCR Multiplexing Capability Using Two Different Strategies

Multiplexing with Droplet Digital PCR (ddPCR) allows for improved sensitivity and precision, as well as increased low-level detection in limited sample types like liquid biopsies. Learn about amplitude and probe-mixing multiplexing and how our ddPCR Assays can help set you up for multiplexing success.
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ArticlesChromatographyFeatured StoriesTechnical Reports

Purification of Antibodies with Nuvia aPrime 4A Hydrophobic Anion Exchange Resin

Our new hydrophobic anion exchange mixed-mode resin, Nuvia aPrime 4A, has been developed to overcome existing challenges in antibody purification. See how we purified an acidic and basic antibody through bind-elute and flow-through modes of purification, respectively, and helped to improve process productivity and economy.
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A Model System for Fast Screening of Optimal Protein Purification Conditions
ArticlesChromatographyTechnical Reports

A Model System for Fast Screening of Optimal Protein Purification Conditions

See how you can efficiently reduce identification of optimal protein purification conditions from five days to just 18 hours. All you need is a DOE model and the Multivariable Scouting tool and automation abilities of the NGC Chromatography System.
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ArticlesChromatographyTechnical Reports

Single-Step Influenza and Dengue Virus Purification with Mixed-Mode CHT Ceramic Hydroxyapatite XT Media

See how you can use CHT XT Mixed-Mode Media to recover over 75% of your virus in a single purification step. All this while reducing contaminating proteins by over 90% and removing over 60% of dsDNA. Get simple, fast, and reproducible viral purification with CHT XT.
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Minimizing On-Column Monoclonal Antibody Aggregate Formation
ArticlesChromatographyTechnical Reports

Minimizing On-Column Monoclonal Antibody Aggregate Formation

The formation of aggregates is a common but highly undesirable occurrence during monoclonal antibody purification. See how on-column aggregation is dependent on the type of resin used and how one particular high-resolution cation exchange resin allows for the lowest proportion of aggregate formation.
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ArticlesReal-time qPCR/PCRTechnical Reports

Parallel Analysis of RNA and Protein Expression Using SingleShot™ Cell Culture Lysates

Comprehensive and effective gene expression studies evaluate both transcriptional and translational regulation. A streamlined protocol was developed for the parallel analysis of mRNA and protein levels using a single lysate produced from cultured cells.
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ArticlesChromatographyTechnical Reports

From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification

A well-designed multidimensional (Multi-D) chromatography process, which utilizes optimized individual column methods, can help automate multistep protein purification processes. This can help you save time and increase productivity. See how you can use Multi-D chromatography to expedite the purification of polyhistidine-tagged proteins when using a typical capture, intermediate, and polish purification workflow.
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ArticlesChromatographyTechnical Reports

Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison of CHT™ Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes

The separation of monomers from product-related impurities is quite challenging in process separations. It requires a thorough screening and an efficient resin to get efficiency in the purification process. Review the performance data of Bio-Rad’s mixed mode CHT™ Ceramic Hydroxapatite in achieving process separation efficiency and how it compares with various Capto media.
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ArticlesDroplet Digital PCR (ddPCR)Technical Reports

A Semi-Automated Quantitative PCR Approach to Assess Differential Anti-Apoptotic Gene Expression Patterns Associated with Prostate Cancer Progression

Gene expression analyses become complicated and extremely time consuming when large numbers of genes need to be analyzed. Here we present a semi-automated qPCR workflow with reduced setup and processing times using Bio-Rad’s PrimePCR™ Panels and CFX Automation System II Plate Handler.
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ArticlesDroplet Digital PCR (ddPCR)Technical Reports

Ultra-Sensitive Quantification of Genome Editing Events Using Droplet Digital PCR

Genome editing using tools such as CRISPR/Cas9 and TALEN has gained enormous popularity in recent days due to their ability to create precise deletions or insertions. However when the frequency of these gene edits are really small, an ultrasensitive method like ddPCR™ is needed for accurate quantification. This technote explains how Droplet Digital™ PCR is used for the detection of HDR and NHEJ alleles generated using CRISPR/Cas9 and TALEN editing systems.
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