ArticlesProduct HighlightsReal-time qPCR/PCR

Introducing the CFX Opus Real-Time PCR Systems —
A Modern Take on Bio-Rad Consistency

Coming soon: The next iteration in Bio-Rad’s tradition of qPCR excellence. The compact CFX Opus Real-Time PCR System is more connected, consistent, and easier to use than ever before, and is available in 96- and 384-well formats. The CFX Opus Systems launch with our new BR.io cloud platform and the latest version of CFX Maestro Software, for complete instrument control and data analysis capabilities.
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ArticlesReal-time qPCR/PCRTechnical Reports

Reference Gene Selection Using PrimePCR Plates and CFX Maestro Software

Reference gene selection and validation are very important for gene expression studies that involve different samples or experimental conditions and therefore are absolutely necessary before any further exploration. See how PrimePCR Reference Gene Selection Panels and CFX Maestro Software provide an easy-to-use system with which to select appropriate reference genes.
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ArticlesProtocols and TipsReal-time qPCR/PCR

Protein Thermal Shift Assays Made Easy with
Bio-Rad’s Family of CFX Real-Time PCR Detection Systems

Protein thermal shift assays enable quick and easy buffer optimization for increased protein stability. See how Bio-Rad’s family of CFX Real-Time PCR Detection Systems can measure protein thermal stability with higher throughput and more buffer systems than traditional circular dichroism detection.
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Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR
ArticlesFeatured StoriesProtocols and TipsReal-time qPCR/PCR

Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR

One-step multiplex RT-qPCR is a technique used to rapidly quantify multiple targets directly from RNA in a single reaction. But proper optimization and validation is essential for its success. These five tips will have you designing primers and probes, selecting reporters, and validating and optimizing your experiments like a pro.
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ArticlesFeatured StoriesReal-time qPCR/PCR

Sensitive Codetection of Viral-Pathogen RNA and DNA
Using One-Step Multiplex RT-qPCR

RT-qPCR is a well-established method for the detection, quantification, and typing of different microbial agents. However, when dealing with the challenges of pathogen detection, not all RT-qPCR reagents are created equal. See how we used a novel one-step multiplex RT-qPCR supermix to demonstrate sensitive codetection of viral RNA and DNA targets in a multiplex setting.
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ArticlesFeatured StoriesReal-time qPCR/PCR

End the Cycle of Repeated, Failed Experiments with a Robust Field Guide to qPCR

qPCR has become ubiquitous for nucleic acid detection and quantification and remains the gold standard for a wide array of applications. To help you publish high-quality, reproducible data that reflect true experimental conditions, we’ve developed this comprehensive guide to performing the ultimate qPCR experiment.
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Multiplex RT qPCR in One Step
ArticlesFeatured StoriesReal-time qPCR/PCR

New Product Feature: Multiplex RT-qPCR in One Step

Using RT-qPCR for RNA, though critical in several industries, is often met with challenges. Many current options don’t allow for multiplexing or high-throughput operation. Bio-Rad’s new Reliance One-Step Multiplex Supermix was designed to grant researchers the expanded, efficient capabilities they need in RT-qPCR. This article presents some of the performance data from the new supermix and describes how exceptional it is at multiplex detection.
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The Universal qPCR Reagent
ArticlesGeneral InterestReal-time qPCR/PCR

One for All — The Universal qPCR Reagent

Passive reference dyes are included in qPCR/RT-qPCR supermixes to compensate for pipetting errors and thermal and/or optical differences in real-time instruments. Our universal line of reagents eliminates the need for spiking in dyes or having to worry about how much ROX your instrument needs. See how your qPCR experiments can also generate more reliable data and be reproducible by anyone.
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Preamplification and How it Can Be Used to Maximize qPCR Data Generation from Limited Samples
ArticlesFeatured StoriesProtocols and TipsReal-time qPCR/PCR

Preamplification and How it Can Be Used to Maximize qPCR Data Generation from Limited Samples

Incorporating preamplification into your qPCR experiments may seem daunting, but it is a powerful way to analyze targets from limited samples. The tips in this article will help you understand how preamplification can enhance your qPCR experiments and the considerations and implications for incorporating it into your workflow.
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ArticlesReal-time qPCR/PCRTechnical Reports

Parallel Analysis of RNA and Protein Expression Using SingleShot™ Cell Culture Lysates

Comprehensive and effective gene expression studies evaluate both transcriptional and translational regulation. A streamlined protocol was developed for the parallel analysis of mRNA and protein levels using a single lysate produced from cultured cells.
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