ArticlesCell Biology

5 High-Throughput Screening Applications Using Flow Cytometry

For many scientists, high-throughput flow cytometry screens are associated with drug or compound libraries, but did you know that you can also apply this technique to study microorganisms, clinical melanoma samples, and even protein trafficking? Read on for five examples of the use of flow cytometry in diverse, high-throughput screening applications.
READ MORE →
ArticlesCell BiologyFeatured StoriesGeneral InterestProtocols and Tips

6 Tips to Secure Funding for Flow Cytometers

We asked core facilities staff and research scientists from the U.S. and Europe for their top tips for securing funding for large instrumentation, such as a cell analyzer or cell sorter. Here are the key questions you should consider while preparing your grant application to increase your chances of a successful outcome.
READ MORE →
ArticlesCell BiologyCOVID-19Drug Discovery and DevelopmentFeatured StoriesGeneral InterestResearch Highlights

Immune Surveillance in SARS-CoV-2 Vaccine Development

The ability to characterize the target of cell-mediated immune response is crucial during vaccine development. In studying the characterization of SARS-CoV-2 infection pathogenesis in humans, a research team identified neutralizing antibodies that provided context for vaccine antigen design. Discover how the ZE5 Cell Analyzer was used to detect activation-induced markers, measure intracellular cytokines, and identify spike protein–specific antibodies in a rapid, high-throughput manner.
READ MORE →
ArticlesCell BiologyResearch Highlights

How FACS, Genetic Screens, and the HAP1 Cell Line Uncover the Biological Mechanisms of Cancer

Genetic screens are powerful tools for finding genes associated with a particular phenotype. They play an especially important role in cancer research and are leveraged to identify predictive biomarkers that can be used to improve treatment efficacy or trace the progression of genetic alterations that ultimately lead to disease. The HAP1 cancer cell line model and fluorescence-activated cell sorting (FACS) bring efficiency to the development of genetic screens that is making them a force to be reckoned with.
READ MORE →
ArticlesCell BiologyProtocols and Tips

7 Tips for Purchasing Your Next Cell Sorter

Presented here are some tips to help you avoid buyer’s remorse and make an educated decision about which instrument to purchase. These tips provide you with the tools to make an informed decision when purchasing a cell sorter.
READ MORE →
ArticlesCell BiologyResearch Highlights

Sorting an Old Problem: Are Cells Stressed by Fluorescence-Activated Cell Sorting?

Fluorescence-activated cell sorting is a valuable tool for research, but could the physical forces of sorting stress the cells, altering their behavior and confounding experimental results? Recent research found that although cell sorting activated p38 MAPK stress signaling, no functional or structural changes were observable. The results indicate that jet-in-air systems such as the Bio-Rad S3e Cell Sorter do not alter cell behavior.
READ MORE →
ArticlesCell BiologyFeatured StoriesProtocols and Tips

10 Tips for Setting Up a Successful Cell Sorting Experiment

Incorporating fluorescence-based cell sorting into your lab grants you powerful access to new research areas and rarer cell types without sacrificing efficiency or results. New, powerful techniques like CRISPR and single-cell analysis are enhanced by the isolation of specific cells. But isolating cell populations of the right quality and quantity is both challenging and time consuming. We offer some tips to set yourself up for success and get the most out of your sorts.
READ MORE →
ArticlesCell BiologyFeatured StoriesGeneral Interest

Improving CRISPR-Cas9 Gene Editing:
Tackling the HDR Inefficiency Problem

A recent publication showed that expression of a protein involved in homology-directed repair (HDR) can improve efficiency of CRISPR-Cas9 gene editing. Learn how manipulation of DNA repair pathways and improvements to gene editing workflows, such as the addition of cell sorting, can facilitate applications of CRISPR-Cas9 technology.
READ MORE →
ArticlesCell BiologyFeatured Stories

GMP-Compliant Production of CAR-T Cells Using mRNA Transfection

Many patients with treatment-resistant blood cancers have found new hope in chimeric antigen receptor (CAR)-T cell therapy. But generation of CAR-T cells at clinical scale can be challenging. Find out how the Gene Pulser Xcell Electroporation System can be used to reduce costs and risks associated with clinical-scale CAR-T cell production, while maintaining GMP compliance.
READ MORE →
ArticlesCell Biology

Turning Back the Clock on Mouse Neural Progenitor Cells

Is the fate of an embryonic cell determined by the age of the cell or the age of the embryo? To explore this question, researchers isolated and transplanted live neural cells from mouse embryos at different stages of development. Learn about their findings and how the S3e Cell Sorter helped achieve this incredible feat.
READ MORE →