Bench PartnersLab NotesReal-Time qPCR/PCR VideoVideos

How to Design Hydrolysis Probes for qPCR

What’s the optimal melting temperature for a hydrolysis probe? How long should your probe be? And why should you avoid 5′ guanines like the plague? This episode answers these questions and gives you five simple tricks for designing the perfect hydrolysis probe.
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Bench PartnersLab NotesReal-Time qPCR/PCR VideoVideos

Six Tips for Increasing the Reproducibility of qPCR Experiments

Follow these six simple tips and you’ll increase the reproducibility of your qPCR experiments.
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Bench PartnersLab NotesReal-Time qPCR/PCR VideoVideos

How to Minimize Contamination in qPCR Experiments

Are you using ethanol to prep your bench for qPCR? Watch this episode to find out why that might be a bad idea. We’re sharing seven tips for minimizing contamination of qPCR experiments.
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Bench PartnersLab NotesReal-Time qPCR/PCR VideoVideos

How to Design Primers for qPCR

How long should a qPCR primer be and what types of sequences should you avoid? In this episode we share eight tips and tricks for primer design for your qPCR experiments.
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Bench PartnersChromatography VideoLab NotesVideos

Four Tips for Developing a Sample Prep Protocol for SDS-PAGE or Chromatography

From making sure that your lysis buffer is compatible with your protein quantitation method to advice on when you do and don’t have to worry about proteases, we share helpful tips for developing a sample preparation protocol.
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Bench PartnersChromatography VideoLab NotesVideos

Five Tips for Picking the Right Cell Disruption Method for Protein Analysis

Sonicator or freeze-thaw? Detergent or enzymatic lysis? Find out how to pick the right cell disruption method for your experiment.
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Bench PartnersElectrophoresis/Western Blotting VideoLab NotesVideos

Four Tips for Handcasting SDS-PAGE Gels

Watch this episode of LabNotes for helpful tips like how to pour the stacking and resolving gel at the same time.
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Bench PartnersElectrophoresis/Western Blotting VideoLab NotesTips and Techniques VideoVideos

Four Tips for Running the Perfect SDS-PAGE Gel

We share five simple tricks that address common gel running woes, from samples that float out of wells to sample buffers that turn yellow.
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Bench PartnersElectrophoresis/Western Blotting VideoLab NotesVideos

Choosing the Right Percentage Gel

Unsure about what percentage SDS-PAGE gel to pick for your experiment? We share four tips that make picking the perfect gel a piece of cake.
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Bench PartnersElectrophoresis/Western Blotting VideoLab NotesVideos

Minimizing Protein Degradation

In this episode, we share tips and tricks for minimizing protein degradation during cell lysis and sample preparation.
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