#1 May the Plex Be with You — Serum or Plasma: What Sample Do I Choose for My Multiplex Biomarker Assays?
Sample measurements can differ for serum and plasma. Why?
- Serum is qualitatively different from plasma because of how the removal of fibrinogen and other proteins that form fibrin clots affects coagulation
- Protein concentration of serum is ~3–4% less than that of plasma
Choose sample matrix measurements that fall within the linear portion of the standard curve. This will allow for better precision and accuracy.
Avoid serum with gross lipemia or hemolysis.