Tip of the Week

#5 General Tips to Make your Multiplex Experiments Easier

Plan your experiment to ensure you use consumables from a single lot.

Consistency is the key:

  • Use low protein binding aliquot tubes to maintain consistent assay performance
  • Dispense reagents using multichannel pipets
  • Ensure you use a lab timer to improve time uniformity between incubation steps in the assay
  • Use dilution tubes to prepare serial dilution
  • Use reverse-pipetting technique to enhance liquid dispensing accuracy
  • Incubate the reconstituted standards on ice for 30 min
  • Resuspend beads thoroughly before removing desired volume

Prepare 25% excess when possible.

Always run your standards, blanks, and samples in duplicates or more.

Strepavidin-PE is light sensitive:

  • Cover the plate with foil during shaking steps
  • Store reagents protected from light

Use a 200–300 μl capacity pipet to remove beads from the stock tube to minimize volume loss. If necessary, perform the volume transfer in two steps.

Previous post

Are You Vegetarian? Could Be Something in Your Genes

Next post

How Do You Automate a Multicolumn Protein Purification?