#5 General Tips to Make your Multiplex Experiments Easier

Plan your experiment to ensure you use consumables from a single lot.

• Use low protein binding aliquot tubes to maintain consistent assay performance
• Dispense reagents using multichannel pipets
• Ensure you use a lab timer to improve time uniformity between incubation steps in the assay
• Use dilution tubes to prepare serial dilution
• Use reverse-pipetting technique to enhance liquid dispensing accuracy
• Incubate the reconstituted standards on ice for 30 min
• Resuspend beads thoroughly before removing desired volume

Prepare 25% excess when possible.

Always run your standards, blanks, and samples in duplicates or more.

• Cover the plate with foil during shaking steps
• Store reagents protected from light

Use a 200–300 μl capacity pipet to remove beads from the stock tube to minimize volume loss. If necessary, perform the volume transfer in two steps.