#2 Steps for a Successful Serum or Plasma Preparations for Multiplex Biomarker Assays

The following steps can help with the success of your multiplex assays.

1. Allow blood to clot at room temperature for 30–45 min before proceeding to centrifugation steps.
2. Centrifuge at 1,000 x g for 15 min at 4°C and transfer into a clean tube.
3. Repeat centrifugation at 10,000 x g for 10 min at 4°C to completely remove platelets and precipitates.
4. Dilute samples according to the assay instruction manual.

1. Centrifuge at 1,000 x g for 15 min at 4°C and transfer into a clean tube.
2. Repeat centrifugation at 10,000 x g for 10 min at 4°C to completely remove platelets and precipitates.
3. Dilute samples according to the assay instruction manual.