#4 Steps to a Successful Cell and Tissue Homogenates Prep for Multiplex Assays
The following steps can help with the success of your multiplex assays.
Steps to a Successful Cell and Tissue Homogenates Prep
- Rinse the tissue sample with cell wash buffer.
If necessary, cut the tissue into small pieces (for example, 3 x 3 mm) and transfer to a tissue grinder on ice.
Dilute lysate at least twofold (1:2) in sample diluent + 0.5% BSA, to a final protein concentration of 200–900 µg/ml.
For analytes with high expression, a lysate protein concentration as low as 50 µg/ml may be sufficient.
Standards should be reconstituted in the same buffer composition as your sample.
Dilute lysates as much as possible to reduce the detergent concentration. This will result in optimum antibody binding during sample incubation period.