#4 How Do You Listen to Your FPLC UV Detector?

Bioradiations Staff — September 3, 2015

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Sawtooth UV signals can be an indication of air trapped in your flow cell. You can often chase the bubble out of your system with 1 N NaOH.

Random spikes in a UV trace that coincide with negative conductivity spikes most often indicate presence of air. Recurring instances of these spikes can often be corrected by purging your system with 1 N NaOH.

Signs of pulsation in your gradient may indicate that you are not using the correct size of mixer for your flow rate.

S-shaped conductivity gradient curves often indicate a mismatch between mixer size and flow rate.

#4 How Do You Listen to Your FPLC UV Detector?

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