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How Do You Automate a Multicolumn Protein Purification?

Purifying proteins using multiple columns is tedious, requiring manual collection of fractions followed by injection into successive columns. In addition to this hands-on time, the process introduces variability, decreasing the success of purification and reproducibility of results. This whiteboard animation explains how the NGC™ Chromatography System uses the multi-dimensional (Multi-D) chromatography method to automate the process, leading to higher protein purification levels and increased reproducibility in your runs. To learn how to automate your multicolumn 2-D and tandem purifications, visit

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