ArticlesDroplet Digital PCR (ddPCR)Drug Discovery and Development

Precise Cell and Gene Therapy Potency Quantification

Inadequate quality control assays shouldn’t limit cell and gene therapy applications. Instead, quantifying the potency of cell and gene therapies is essential for successful therapeutics. In this article, we describe multiple leading potency assays developed using Droplet Digital PCR (ddPCR) technology, including an assay that quantifies transgene integration and expression in one step.
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Real-Time qPCR/PCR VideoVideos

The CFX Duet Real-Time PCR System: Our Most Accessible qPCR Technology

Through the CFX Duet Real-Time PCR System, our newest generation of quantitative PCR (qPCR) instrumentation, this technology is now even more accessible for molecular biologists and other professionals doing essential qPCR. With CFX Maestro Software, you can perform duplex detection, gene expression, and genotyping, all without paying for additional optics you don’t need. Check out our video tour of the new CFX Duet System.
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Next-Generation Sequencing VideoProduct HighlightsVideos

Take the Expressway to RNA Library Prep

RNA-Seq library preparation is a manually intensive, time-consuming process prone to bias from the multiple enzymes required. So, how can you trust your sequencing results? Bio-Rad is revolutionizing RNA-Seq library prep with the SEQuoia Express Stranded RNA Library Prep Kit – a three-hour, three-tube solution eliminating the need for multiple enzymes and reducing both the number of pipetting steps and protocol time. The key is an engineered, proprietary retrotransposon called SEQzyme. With this serial template jumping, or continuous synthesis, reaction you can obtain an unbiased, stranded RNA-Seq library constructed to accurately represent both messenger and non-coding RNA from your sample.
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High Performance with GMP Ready CHT Prepacked Process-Scale Columns

Foresight Pro CHT Prepacked Columns are GMP ready for process-scale purification. Learn about the design and see performance data in this webinar.
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Introducing the NGC™ Fraction Collector for Discovery to Process-Scale Protein Purification
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Introducing the NGC™ Fraction Collector for Discovery to Process-Scale Protein Purification

The newly launched NGC™ Fraction Collector, together with ChromLab™ 5.0 Software, have enhanced the functionality of the NGC Chromatography System to provide the flexibility and collection capacity needed for any application, from discovery to scale up. See how the NGC System can help you improve your protein purification process.
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An IMAC Resin for a Highly Productive Histidine-Tagged Protein Purification Process

One of the advancements in protein purification has been to tag the protein of interest with a short sequence that can bind to an immobilized matrix and aid purification. Bio-Rad’s new IMAC resin offers a host of advantages for the purification of histidine-tagged proteins. Learn more about this resin and see how it can enhance the efficiency of your protein purification workflow.
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Get More Answers from Your Mouse with the Bio-Plex Pro™ Mouse Chemokine Panel

Biomarker screening can sometimes be like working in a black box. While traditional research tools focus on one protein at a time, Bio-Rad’s new Bio-Plex Pro™ Mouse Chemokine Panel can simultaneously interrogate 33 chemokines and cytokines in a single well. See how you can get more answers with this panel.
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ArticlesElectrophoresis/Western BlottingFeatured StoriesProduct HighlightsProtocols and TipsReal-time qPCR/PCR

A Superior Method for Parallel Analysis of RNA and Protein Expression from Cell Culture Lysates

It is imperative to analyze both the transcriptional and translational profiles for a complete understanding of the functioning of genes. Here we describe a simple protocol that enables parallel analysis of both RNA and protein from single cell culture lysates. See how this workflow could save time and money and yield more reliable data than the traditional protocols used.
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Purifying Difficult-to-Purify Proteins

Protein purification becomes a challenging step for applications requiring high protein concentrations (such as structural biology studies). This is also true for researchers purifying integral membranes proteins and untagged proteins from native sources. Here we provide some useful tips for selecting the proper resins, optimizing conditions and developing effective strategies to tackle the challenges associated with such difficult-to-purify proteins.
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ArticlesProduct HighlightsReal-time qPCR/PCR

The Importance of Quality PCR Plastics

Do we ever think seriously about PCR plates, seals, and tubes as contributing to the quality of our gene expression results? Chances are that these PCR plastics are viewed just as labware by most people. In this article we bring out how simple factors, such as PCR plate rigidity, color of the wells, and adhesiveness of plate seals, can affect your gene expression results and show why it is important to use quality PCR plastics.
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