Bio-Plex Pro™ mouse Th17 assays are magnetic bead–based multiplex assays that offer accurate and reproducible measurements of 20 target molecules in serum, plasma, and tissue culture sample types for mouse models. These assays have been developed to provide reliable performance with the flexibility required to meet all of your research needs.

Features and Benefits

  • Broad assay range — optimized to detect analyte levels in normal and diseased samples
  • Robustness — validated in serum, plasma, and tissue culture
  • Increased productivity — measure multiple analytes simultaneously in one 3 hr experiment
  • Flexible ordering options — order either a premixed panel, singleplex analytes of interest, or customize your assay with Bio-Plex® assay builder (www.bio-rad.com/assaybuilder)
  • Option to use a magnetic wash station — simplify your assay workflow and improve data consistency among experiments
  • Compatibility with Bio-Plex 200 and Bio-Plex 3D systems

br133_ann_th17_table_1_v2

*  IL-25 and IL-27p28 were measured as a 2-plex.
** ICAM-1 was measured as a singleplex.

Representative assay working ranges, sensitivity, and precision. The LLOQ, ULOQ, LOD, and inter-assay precision %CV are mean data determined from three independent multiplex assays in a serum-based matrix. Intra-assay %CV is derived from one representative assay. LLOQ and ULOC are defined as the boundary standard curve points in which the performance specifications of individual standard points were met for 10% intra-assay CV, 15% inter-assay CV, and a recovery range of 80–120%. Data were generated using the magnetic workflow with the Bio-Plex II wash station.

 

Ordering Information

Catalog #Description
M60-00007NY Bio-Plex Pro Mouse Cytokine Th17 Panel A 6-Plex Group l, 1 x 96-well, includes premixed coupled magnetic beads and detection antibodies, standard, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, filter plate, flat-bottom plate, sealing tape, standard diluent, sample diluent, and instructions for the detection of 6 cytokine biomarkers (IL-1β, IL-6, IL-10, IL-17A, IFN-γ, TNF-α)
171-FA001M Bio-Plex Pro Mouse Cytokine Th17 Panel B 8-Plex Group lll, 1 x 96-well, includes premixed coupled magnetic beads and detection antibodies, standard, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, filter plate, flat-bottom plate, sealing tape, standard diluent, sample diluent, and instructions for the detection of 8 cytokine biomarkers (IL-17F, IL-21, IL-22, IL-23p19, IL-31, IL-33, CD40L, MIP-3α)

The Bio-Plex suspension array system includes fluorescently labeled microspheres and instrumentation licensed to Bio-Rad Laboratories, Inc. by the Luminex Corporation.

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