Biologically interesting RNAs, such as mRNA, miRNA, lncRNA, and piRNA, comprise a small fraction of total cellular RNA. Ribosomal RNA, on the other hand, makes up 80–90% of the RNA pool but is typically of little interest. To prevent ribosomal RNA fragments from dominating an RNA-Seq library and taking up unnecessary space on a sequencing chip, they must be depleted before sequencing.
The SEQuoia RiboDepletion Kit employs novel technology to deplete ribosomal RNA while preserving rare transcripts and small RNAs, as well as minimizing the risk of degrading or low abundant samples. The SEQuoia RiboDepletion Kit also offers a streamlined workflow and multiplex compatibility, leading to significant time and cost savings compared to traditional methods. Watch this video to learn more.