Researchers Yiping Cao and John Griffith of the Southern California Coastal Research Project describe their work with Droplet Digital™ PCR (ddPCR™) technology to create a new assay for detecting harmful pathogens in environmental water samples. They discuss how ddPCR affords them the ability to obtain absolute quantification of multiple indicator organisms, and how this improves upon existing methods.

The QX200™ Droplet Digital PCR system, Bio-Rad’s second-generation digital PCR system, provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan hydrolysis probes, yielding unmatched sensitivity and precision for a wide variety of applications.

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