The new CFX96 Touch Deep Well real-time PCR detection system joins Bio-Rad’s real-time PCR detection system family as the system for large-volume qPCR reactions (up to 125 µl). The system can accurately discriminate up to five targets in a single reaction well by using Bio-Rad’s solid-state optical technology (Figure 1). The optical system scans just above the sample plate to illuminate and detect from each well with high sensitivity and no cross talk.
In addition to using industry-leading optical technology, the system offers excellent thermal performance and uniformity for large-volume reactions by using Bio-Rad’s patented reduced-mass sample block (Figure 2). This sample block heats and cools more quickly than standard blocks, so average ramp rates are increased and overall run times are reduced. The system also incorporates a built-in thermal gradient for easy assay optimization in a single run.
The CFX96 Touch Deep Well system includes the easy-to-use C1000 Touch™ chassis to create a truly stand-alone qPCR system. Runs can be quickly and easily set up using the intuitive LCD touch screen menus (Figure 3) and amplification traces can be viewed on the LCD screen in real time while a run is in progress (Figure 4) providing a truly stand-alone qPCR system.
Data analysis can be performed using the powerful CFX Manager™ software. The software offers easy run setup and advanced gene expression data grouping tools such as bar chart, clustergram, scatter plot, volcano plate, and heat map (Figures 5 and 6) for all Bio-Rad’s real-time PCR detection systems.
The following chart displays the specifications of all available real-time PCR detection systems from Bio-Rad.
|MiniOpticon™||CFX Connect||CFX96 Touch||CFX96 Touch Deep Well||CFX384 Touch|
|Base thermal cycler||MJ Mini™||CFX Connect||C1000 Touch™||C1000 Touch||C1000 Touch|
|Sample volume||Up to 100 µl||Up to 50 µl||Up to 50 µl||Up to 125 µl||Up to 30 µl|
|Excitation range||470–500 nm||450–535 nm||450–684 nm||450–684 nm||450–650 nm|
|Multiplex capability||Up to 2 targets||Up to 2 targets||Up to 5 targets||Up to 5 targets||Up to 4 targets|
|1854096||CFX96 Touch Deep Well Real-Time PCR Detection System, includes C1000 Touch thermal cycler chassis, CFX96 Deep Well™ optical reaction module, CFX Manager software, license for qbasePLUS software, communication cable, reagents, consumables|
|1854095||CFX96 Touch Deep Well Real-Time PCR Detection System, includes C1000 Touch thermal cycler chassis, CFX96 Deep Well optical reaction module, CFX Manager software, license for qbasePLUS software, communication cable|
|1844096||CFX96 Deep Well Optical Reaction Module, for use with C1000 Touch thermal cycler chassis, includes CFX Manager software, license for qbasePLUS software, communication cable, reagents, consumables|
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