Understanding the Long Noncoding Transcriptome
Real-Time qPCR/PCR VideoVideos

Discover lncRNA: Understanding the Long Noncoding Transcriptome

Learn about the exciting new field of long noncoding RNA (lncRNA) and how this newly discovered class of RNA compares to messenger RNA (mRNA).
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ArticlesProduct HighlightsReal-time qPCR/PCR

New CFX96 Touch™ Deep Well Real-Time PCR Instrument Enables Large-Volume qPCR Reactions

Large sample volumes may sometimes be needed to conduct gene expression studies. Bio-Rad’s new CFX96 Touch Deep Well™ real-time PCR detection system can run sample volumes up to 125 µl and can discriminate up to five targets simultaneously.
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ArticlesDroplet Digital PCR (ddPCR)Product Highlights

A New Paradigm for Precise Quantitation of RNA

The one-step RT-ddPCR kit for probes creates a new paradigm for the precise quantitation of RNA by combining reverse transcription with Droplet Digital™ PCR (ddPCR™), providing an absolute measure of target RNA molecules with unrivaled precision and sensitivity
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ArticlesReal-time qPCR/PCRTechnical Reports

A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data

This tech report shows how qPCR can lead to erroneous conclusions regarding differences in MCM7 gene expression between normal and tumor human breast cancer samples if the key steps set out in the MIQE guidelines are not followed.
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ArticlesCell BiologyResearch Highlights

Targeting Suppression of B-Cell Lymphoma Proliferation

Rossi considers the discovery of RNAi as pivotal to antiviral discovery. “RNAi has become another important, powerful genetic tool we can use to further our antiviral work,” he says. Rossi’s lab became the first to publish research demonstrating the expression of small interfering RNAs (siRNAs) to inhibit HIV replication in human cells. Various aspects of RNAi and siRNAs continue to play a key role in his research on viral diseases and lymphomas.
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ArticlesCell BiologyCustomer Stories

Unlocking the Potential of Stem Cell Research Using the EpiQ™ Chromatin Analysis Kit

Bedtime stories in Dr David Schaffer’s — one of today’s pioneers in stem cell research — childhood home were often not standard fairy tales. With both parents in careers as biomedical researchers (his mother in drug development and clinical trials for a major pharmaceutical company and his father, in cardiovascular research and a pharmacology professor) much of the conversation as far back as Schaffer can remember centered on biology and science.
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ArticlesReal-time qPCR/PCRTechnical Reports

All cDNA Synthesis Kits Are Not Created Equal: RNase A Inhibition and Its Effect on qPCR Data

Not all cDNA synthesis kits have the same capacity to protect the integrity of RNA. An optimal level of RNase inhibitor is critical for powerful RNase inhibition and efficient reverse transcription. The iScript™ cDNA synthesis kit comes with a 5x iScript reaction mix and iScript reverse transcriptase, which is also blended with a potent RNase inhibitor.
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