Any transcript. Any sample. Any amount.
The SEQuoia Complete Stranded RNA Library Prep Kit is the newest addition to Bio-Rad’s next-generation sequencing (NGS) space. The high-performance kit captures both long and short RNAs in a single library, even from limited and low-quality samples. Featuring SEQzyme, a proprietary engineered enzyme that couples cDNA synthesis with adaptor addition in a one-tube continuous synthesis reaction, this kit allows construction of libraries that are >99% stranded and suitable for next-generation sequencing on Illumina® platforms.
See why the SEQuoia Complete Kit is your top choice for high-performance RNA-Seq library prep.
Validated with FFPE and other low-quality samples. Gene discovery from limited formalin-fixed paraffin-embedded (FFPE) samples exhibits high concordance with matched frozen samples. 85% of genes identified in SEQuoia libraries from FFPE samples were also found in libraries from frozen samples, highlighting the biological relevance of the FFPE sequencing results obtained using the SEQuoia Complete Stranded RNA Library Prep Kit.
Captures long and short RNAs in a single prep. The unique enzymatic properties of SEQzyme effectively capture fragments from highly degraded RNA and short RNA fragments (for example, snoRNA, miRNA, and tRNA), which are commonly missed by other commercial RNA library prep kits. The efficient simultaneous capture of diverse RNA fragments results in a library that more accurately represents the complete transcriptome.
Performs well with low sample input. The comprehensive, diverse libraries constructed from a wide range of starting materials produces substantially higher yields with fewer PCR cycles than other commercial kits when low quantities of degraded RNA are used as input.
Enables an efficient and streamlined library prep workflow. The kit is optimized to facilitate efficient library synthesis using a wide range of input RNA (100 pg to 1 μg) and is compatible with automated next-generation sequencing library construction. The streamlined workflow minimizes the number of pipetting steps and greatly reduces the overall protocol time to less than 4 hours.
Experience the power of the SEQuoia Complete Kit today.