Minimize On-Column Aggregates during mAb Purification

divider
the challenge

The Challenge

Have you seen two-peak elution of target monoclonal antibodies (mAb) after cation exchange (CEX) chromatography? The second elution peak consists of a mixture of monomers and aggregates of your target mAb and it leads to a decrease in your process yield.

divider
purification tip

Purification Tip

Choosing the optimal resin for your process will minimize on-column aggregates and maximize recovery. Be sure to screen multiple resins during your mAb purification process development.

divider
our approach

Our Approach

On-column aggregate formation depends on the specific chromatography resin used. Resin structure, surface extenders, pore size and distribution each contribute to on-column aggregate formation. Bio-Rad’s Nuvia HR-S Resin — a strong cation exchanger with a pore size of 50 µm without surface extenders — minimizes on-column aggregates. Nuvia HR-S generates only 10% aggregate formation relative to competing resins under similar experimental conditions.

divider

See how your resin-of-choice fares in on-column aggregate formation during monoclonal antibody purification.

Select resins by clicking on the buttons on the left.

 

%, on-column aggregates

0 min hold time

1,000 min hold time
44%

Eshmuno S

50%

Capto SP ImpRes

51%

POROS 50 HS

65%

Capto S ImpAct

87%

Fractogel SO3

10%

Nuvia HR-S

 

Additional Information

Minimize on-column aggregate formation by proper resin screening and subsequent optimization of resin type.

Learn more about the high-resolution Nuvia HR-S Cation Exchange Resin.

Request a sample of Nuvia HR-S Resin.

Bio-Rad is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. All trademarks used herein are the property of their respective owner.

Previous post

Defining the New Normal in Quantitative Western Blot Data

Next post

10 Tips for Western Blot Detection of Proteins Present in Tissue Lysates