Minimize On-Column Aggregates during mAb Puriﬁcation
Have you seen two-peak elution of target monoclonal antibodies (mAb) after cation exchange (CEX) chromatography? The second elution peak consists of a mixture of monomers and aggregates of your target mAb and it leads to a decrease in your process yield.
Choosing the optimal resin for your process will minimize on-column aggregates and maximize recovery. Be sure to screen multiple resins during your mAb puriﬁcation process development.
On-column aggregate formation depends on the speciﬁc chromatography resin used. Resin structure, surface extenders, pore size and distribution each contribute to on-column aggregate formation. Bio-Rad’s Nuvia HR-S Resin — a strong cation exchanger with a pore size of 50 µm without surface extenders — minimizes on-column aggregates. Nuvia HR-S generates only 10% aggregate formation relative to competing resins under similar experimental conditions.
See how your resin-of-choice fares in on-column aggregate formation during monoclonal antibody purification.
Select resins by clicking on the buttons on the left.
Capto SP ImpRes
POROS 50 HS
Capto S ImpAct