ArticlesChromatography VideoVideos

Two-Step Purification of Low-Expressing Recombinant Exoprotein A Using Mixed-Mode Chromatography

Purifying proteins that are not highly expressed can be challenging. Watch this video to learn about a case study demonstrating an efficient workflow for two-step purification of a low-expressing recombinant protein using mixed-mode chromatography.
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ArticlesDrug Discovery and DevelopmentGeneral Interest

Advanced Cancer Therapeutics: Increasing Efficacy and Specificity and Reducing Toxicity

Research into human diseases has led to the development of novel monoclonal antibody drugs. These monoclonal antibody therapies have been successful because of their specificity and selectivity. However, ongoing development and successfully launching a new mAb drug require robust and reliable methods to screen for drug targets, demonstrate drug safety and efficacy, and scale up manufacturing to meet the stringent regulatory guidelines required for approval.
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ArticlesChromatographyTechnical Reports

Purification of Untagged Proteins Made Attainable for Any Researcher

High purity protein is a common requirement for biochemical and structural studies. A common approach is to recombinantly express an affinity-tagged version of the protein of interest. However, this is not always a viable option. Here we showcase how the NGC Chromatography System and prepacked chromatography columns can be utilized to develop purification workflows for untagged proteins.
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ChromatographyPast WebinarsWebinars

Automate Your Antibody Purification Workflow at Preparative and Analytical Scale

Presented by: Katie Schaefer, PhD, Global Product Manager, Bio-Rad
Peter Winship, PhD, Technical Product Manager, Teledyne Cetac Technologies

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Purification is a multistep process toward confirmation of a target protein’s purity. In this webinar you will learn how to automate your protein purification workflow by following the creation of a hypothetical monoclonal antibody (mAb). Dr. Schaefer and Dr. Winship expertly demonstrate how the NGC Chromatography System and the AEX-500 series Autosampler work together to maximize screening capability as well as rapidly achieve therapeutic development objectives.

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ArticlesChromatographyDrug Discovery and Development

Serving your Downstream Purification Needs

The Process Chromatography Team at Bio-Rad offers a variety of resources to address scientists’ needs during the downstream purification process. Visit our robust online library, which contains application notes, tutorials, webinars, and more, or contact us to speak with a specialist or request a customized seminar.
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ArticlesChromatography

How to Make a Successful Vaccine

As phase 3 clinical trials of investigational vaccines for COVID-19 begin in the U.S., you may be wondering what it takes to make an effective vaccine? In case you missed it, check out our Hanging with My Chromies podcast, episodes 6 and 10 on vaccine development and viral spread, and learn about the role chromatography plays in making a successful vaccine.
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ChromatographyPast WebinarsWebinars

Biosimilar Purification with Ion Exchange and Mixed-Mode Resins

Presented by: Randy Drevland, PhD, Senior Applications Scientist

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Dr. Drevland presents a viable alternative workflow for the development of a HUMIRA-like biosimilar using IEX and mixed-mode resins.

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ArticlesChromatography VideoCustomer Stories

Maximizing the Efficiency of Production Workflows with NGC Chromatography Systems

See how an antibody expression service provider, Evitria (Zurich, Switzerland) optimized and expanded their antibody production services using the NGC Chromatography Systems to meet the growing demands of their customers.
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A Model System for Fast Screening of Optimal Protein Purification Conditions
ArticlesChromatographyTechnical Reports

A Model System for Fast Screening of Optimal Protein Purification Conditions

See how you can efficiently reduce identification of optimal protein purification conditions from five days to just 18 hours. All you need is a DOE model and the Multivariable Scouting tool and automation abilities of the NGC Chromatography System.
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Minimize On-Column Aggregation during mAb Purification
ArticlesChromatographyProtocols and Tips

Got Purification Troubles?

Use our interactive infographic to find out how your resin-of-choice fares in on-column aggregate formation during monoclonal antibody purification. And find out what you can do to minimize these on-column aggregates.
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