Bioradiations.com continues to be an online technical resource for Bio-Rad customers, offering a variety of general interest articles, customer stories, protocols and tips, research and product highlights, and technical reports throughout the year. The following articles were the most popular in 2016. Explore them all to get an idea of what people found interesting this year.

Defining the New Normal in Quantitative Western Blot Data

Normalization of western blot data is very crucial in quantitating proteins. Normalization of band intensity of proteins of interest with the band intensity of housekeeping proteins (HKP) is routinely done in labs. But more and more journals question the validity of using HKPs in normalization and are demanding more validations and tests. An alternative method is to use total protein normalization (TPN) for normalization. Explore the TPN option and see how you can get reliable western blot data easily.
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Assessing Cell Health: Autophagy

Part III of our cell health series describes the three main autophagy mechanisms and reviews the various methods for assessing macroautophagy and autophagic flux in cells.
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From megaTALs to CRISPR: The Many Ways to Edit a Gene

CRISPR has revolutionized gene editing but is not the only way to edit a gene. We compare the pros and cons of four main gene editing techniques – meganucleases, zinc finger nucleases, TALENs, and CRISPR-Cas9 – discuss the advance of these methods to the clinic for therapeutic applications, and introduce newer technologies that eliminate some of the pitfalls of current techniques.
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Assessing Cell Health: Apoptosis

In this second part of the series on assessing the health of your cells, we review the different types and stages of apoptosis, and the various methods of assessing apoptosis of cells.
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Western Blot Detection without Interfering IgGs: A Mere Fantasy or a Real Possibility?

Interference from IgG heavy (~ 50 kD) or light (~25 kD) chains is very common when trying to visualize bands of immunoprecipitated proteins. If your band of interest has a molecular weight close to these, it becomes really hard to distinguish between the IgG and the band of interest. How could this be avoided? Bio-Rad’s new TidyBlot™ Western Blot Detection Reagent accomplishes this by binding exclusively to the native nondenatured antibodies rather than any IgG in the sample. Explore the performance characteristics of this new reagent.
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A Superior Method for Parallel Analysis of RNA and Protein Expression from Cell Culture Lysates

It is imperative to analyze both the transcriptional and translational profiles for a complete understanding of the functioning of genes. Here we describe a simple protocol that enables parallel analysis of both RNA and protein from single cell culture lysates. See how this workflow could save time and money and yield more reliable data than the traditional protocols used.
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Design for Learning: Developing Curricula for Instructional Lab Kits

How are educational curricula developed? Who sets the standards? How do companies that develop science education kits keep up with this and design their kits around the curricula? Find out in this article.
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New Product Feature: gDNA-Free cDNA in Two Simple Steps

Removing genomic DNA contamination from cDNA preparations is a challenge. All currently available methods are time consuming, could lead to sample loss, and are not efficient. Bio-Rad’s iScript™gDNA Clear cDNA Synthesis Kit tackles these issues and makes this step effortless. This article presents the performance data of the kit and describes how simple and efficient it is to use the kit for gDNA removal.
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Rapid Advances in Biotechnology Bring Questions about Patentability

Striking a balance between encouraging innovation and allowing access to knowledge is not simple. Allowing patents for modifications of naturally occurring organisms and cells pose a particular challenge to the scientific community. Adding to the complexity is the variations in regulations between countries. This article discusses these issues with specific examples in biotechnology and biomedical fields.
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Expanding Flow Core Capabilities by Shifting Routine Sorts to Self-Sort on the S3e™ Cell Sorter

To run a core flow cytometry lab is no easy task. There is always a balance between running simple sorts and developing new assays for answering complicated questions that demand pushing the limits of existing assays and the limitations of the sorters. See how Andy Riddell, a flow core manager, pushes the limits of his existing assays and how Bio-Rad’s S3e™ cell sorter is allowing him to accomplish that.
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The Making of Recombinant Anti-Idiotypic Antibodies For High Performance in Bioanalytical Assays

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CRISPR: Changing the Pace of BioPharma R&D