Learn how to improve your western blotting results with Bio-Rad’s Quick Tips video series. These 1–2 minute videos show you how to perfect each step of the western blotting process and were designed to accompany the in-depth curriculum available in the Western Blot Learning Center.

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How to Load a Mini-PROTEAN TGX Stain-Free Gel for Electrophoresis

The Mini-PROTEAN Tetra Cell is a vertical mini gel electrophoresis system designed for fast, simple setup. Learn how to correctly load a Mini-PROTEAN TGX Stain-Free Gel in a Mini-PROTEAN Tetra Cell Electrophoresis Chamber. The unique formulation of Stain-Free TGX Gels allows for decreased run times (15 minutes) while maintaining high sample resolution. Apply the techniques in this video to minimize the chance of having a leaky upper buffer chamber in your next set of western blotting experiments.

How to Minimize Band Distortion During Gel Electrophoresis for Western Blotting

Uneven sample migration in polyacrylamide gel electrophoresis (PAGE), also called smiling and frowning, can make it difficult to accurately estimate band size and differentiate between bands on your western blot. Watch this video to learn about common causes of uneven sample migration. These tips will help ensure that you get the best possible resolution in your western blotting experiments.

How to Prepare Mini-PROTEAN TGX Stain-Free Precast Gels for Western Transfer

Watch this video to learn how to safely release your Mini-PROTEAN TGX Stain-Free Gel from the gel cassette. Depending on your downstream application, learn the best technique to prepare your gel for staining, imaging, or blotting.

How to Assemble a Trans-Blot Turbo Transfer Pack for Western Blot Transfer

In this video, we show how simple it is to assemble a blotting sandwich and perform western transfer using the Trans-Blot Turbo Transfer System. This technique combines traditional blotting techniques with modern filter paper and buffers, allowing transfer of proteins from gels to membranes in as little as three minutes, with minimal preparation time. The entire system is in a ready-to-use format that allows researchers to obtain their results faster and easier, with reproducibility that is difficult to achieve using traditional blotting methods.

How to Set up a Mini Trans-Blot Cell for Western Blot Transfer

The Mini Trans-Blot Cell provides rapid, high-quality blotting of Mini-PROTEAN Gels. A component of the Mini-PROTEAN Tetra Electrophoresis System, the Mini Trans-Blot Cell accommodates two gel holder cassettes for electrophoretic transfer of mini format gels run in the Mini-PROTEAN Tetra Cell. Watch this video for a step-by-step tutorial on how to properly set up a Mini Trans-Blot Cell blotting sandwich for efficient transfer.

Optimizing the Blocking Step in Western Blotting

The blocking step in western blotting is a critical process. An optimized block can increase your blot sensitivity while drastically reducing background and nonspecific binding. In this video, we explore considerations for a good block and common pitfalls to avoid when selecting a blocking reagent. Keeping these tips and techniques in mind during your next western blotting experiment will help you achieve optimal results the first time.

Selecting a Primary Antibody to Get the Best Results in Western Blotting

Choosing a primary antibody can be overwhelming. Watch this video to learn what to expect and what to look out for when choosing a primary antibody, including how to navigate antibody validation data. Follow these tips when selecting a primary antibody to ensure optimal experimental results.

How to Choose Primary Antibodies for Multiplex Western Blotting

Taking the time to select the best primary antibodies for multiplex western blotting goes a long way toward the success of your experiment. Choosing incompatible antibodies will result in crosstalk or nonspecific binding. To learn best practices for choosing antibodies for your next multiplex western blotting experiment, watch this video.

How to Choose Secondary Antibodies for Multiplex Western Blotting

Knowing how to select secondary antibodies for multiplex western blotting is critical for generating optimized data. Watch this video for an in-depth look into best practices and techniques, enabling you to achieve the best possible results.
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