ArticlesElectrophoresis/Western BlottingProtocols and Tips

Resolved — Comic Series

Follow along as a new starry-eyed graduate student learns the ins and outs of life as a research scientist, gaining valuable western blotting tips and tricks. Along for the ride are a seasoned postdoctoral fellow and traditional principal investigator.
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Electrophoresis/Western BlottingPast WebinarsWebinars

Advancements in Western Blotting Technology: A Western Workflow That Delivers More Precise, Accurate, and Reproducible Results

Presented by: Sean C. Taylor, MBA, PhD, Field Application Scientist Manager

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Learn how to produce journal-ready reproducible and quantitative western blot data.

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Discover Our Capabilities
ArticlesCell Biology VideoChromatography VideoElectrophoresis/Western Blotting VideoReal-Time qPCR/PCR Video

Discover Our Capabilities

From flow cytometry to chromatography to western blot imaging to PCR plastics, learn more about Bio-Rad and see what’s possible in your research.
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10 Tips for Western Blot Detection of Phosphorylation Events
ArticlesElectrophoresis/Western BlottingProtocols and Tips

10 Tips for Western Blot Detection of Phosphorylation Events

Being able to interrogate protein phosphorylation events and aberrant phosphorylation levels is critical to our understanding of various biological and pathological processes. However, detection of phosphorylated proteins in western blots can be challenging. These ten tips will help you generate robust data and cleaner blots.
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Electrophoresis/Western BlottingPast WebinarsWebinars

See the Signal — Illuminating the Pathway to Confident Western Blot Detection of Phosphorylated Proteins

Presented by: Rachael Preston, PhD, Applications Scientist, Bio-Rad Laboratories

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The most common method for assessing the phosphorylation state of a protein is western blotting. In this webinar we talk you through how to fine-tune your western blot protocol to detect phosphorylation events and generate publication-quality western blots.

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Western Blot Detection of Proteins Present in Tissue Lysates
ArticlesElectrophoresis/Western BlottingProtocols and Tips

10 Tips for Western Blot Detection of Proteins Present in Tissue Lysates

Extracting proteins from tissue lysates for western blotting can be a challenge. From knowing how to process your tissue to verifying protein loading to including suitable controls, there is a lot to keep track of. The ten tips in this article can help you on your way to generating reproducible and reliable blots and do better western blotting overall.
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ArticlesElectrophoresis/Western BlottingGeneral Interest

Defining the New Normal in Quantitative Western Blot Data

Normalization of western blot data is very crucial in quantitating proteins. Normalization of band intensity of proteins of interest with the band intensity of housekeeping proteins (HKP) is routinely done in labs. But more and more journals question the validity of using HKPs in normalization and are demanding more validations and tests. An alternative method is to use total protein normalization (TPN) for normalization. Explore the TPN option and see how you can get reliable western blot data easily.
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Publishing Meaningful Antibody Data
ArticlesCell BiologyElectrophoresis/Western BlottingProtocols and Tips

Finding a Good Antibody:
Part III – Publish Meaningful Data

In the first two articles in our Finding a Good Antibody series, you learned how to select the best antibodies and received tips on proper antibody validation. In this third and final article, see how you can use this information to publish meaningful data when using antibodies. And get some guidelines for antibody best practices.
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Antibody Validation for Your Application
ArticlesCell BiologyElectrophoresis/Western BlottingProtocols and Tips

Finding a Good Antibody:
Part II — Antibody Validation

Finding antibodies that work for your application is just the first step in finding a good antibody. Next is antibody validation — making sure that your antibody does what you think it is supposed to. In this second article in our Finding a Good Antibody series, learn how best to go about validating your antibodies.
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Electrophoresis/Western BlottingPast WebinarsWebinars

Best Practices for the Best Western Blots

Presented by: Paul Liu, PhD, Product Manager, Western Blot Reagents and Devices, Protein Quantitation Marketing
Date: Tuesday, July 10, 2018
Time: 10 AM, U.S. Pacific time

Western blotting is an essential and ubiquitous method for protein research. We will discuss method optimization, data analysis best practices, and new advancements in imaging and fluorescent detection reagents that make western blot data more reproducible and quantitative.

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