The How and Why of Normalizing Your Western Blots
ArticlesElectrophoresis/Western BlottingFeatured Stories

The How and Why of Normalizing Your Western Blots

Introducing data normalization into your western blotting workflow may sound unnecessary but it has proven to be a great way to ensure great results. Follow along as we break down some of the main takeaways from the first chapter in Dr. Oh’s popular wester blotting webinar series, including a unique method to ease normalization woes and worries.
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Electrophoresis/Western BlottingPast WebinarsWebinars

Generating Semi-Quantitative Western Blot Data Using Bio-Rad’s Image Lab™ 6.0 Software

Presented by: Kenneth J. Oh, PhD, Applications, Collaborations, and New Technology Manager, Protein Quantitation Marketing Group, Bio-Rad Laboratories, Inc.
Date: Tuesday, March 6, 2018

Western blotting is a popular tool for protein quantitation, and the advent of digital imaging has greatly increased the sensitivity, dynamic range, and quantitation limits of this technique. In the second webinar in this series, get step-to-step instructions for normalizing western blot data using Image Lab 6.0 Software.

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Past WebinarsWebinars

Western Blot Normalization Methods

Presented by: Kenneth J. Oh, PhD, Applications, Collaborations, and New Technology Manager, Protein Quantitation Marketing Group, Bio-Rad Laboratories, Inc.
Date: Tuesday, October 24, 2017

Western blotting continues to be a ubiquitous tool for the identification and quantitation of protein(s) of interest. The first webinar in this series will focus on common normalization techniques for quantitative western blotting and discuss important considerations when using these techniques.

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ArticlesElectrophoresis/Western BlottingResearch Highlights

Trends in Protein Separation and Analysis — the Advance of Stain-Free Technology

What is stain-free technology? How does it work in electrophoresis and western blotting? How does it compare with existing staining techniques? What are its advantages and possible concerns? Here is a technical review of this rapidly growing technological advance.
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ArticlesCustomer StoriesElectrophoresis/Western BlottingFeatured Stories

Telling the Story of Western Blots with the ChemiDoc Touch Imaging System

For a long time X-ray film has been the standard for imaging western blots, but this method has its limitations. Find out how 3 researchers are using the digital tools offered by the new ChemiDoc Touch Imaging System to beat these limitations and improve their blot images.
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ArticlesElectrophoresis/Western BlottingProduct Highlights

Brighter Precision Plus Protein™ Dual Color Standard — The Brightest Protein Marker Available

Take a quick look at Bio-Rad’s brighter Precision Plus Protein™ Dual Color Standard with this article comparing this standard to other available protein markers. Plus, find out why the ability to visualize a protein standard after reprobing matters to one researcher!
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Past WebinarsWebinars

Single-Cell Western Blot and Stain-Free Total Protein Loading Control

Dr. Robyn Murphy of Latrobe University introduces a protocol using Bio-Rad’s stain-free gel technology to conduct western blotting on sample sizes as small as single muscle cells. Dr. Murphy shows how this reliable western blotting method can be a valuable research tool. Learn more about stain-free technology.  
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Past WebinarsWebinars

Can We Trust Western Blots?

Prof. Aldrin Gomes of UC Davis shares western blot problems he has seen as a journal editor and outlines standardized procedures and controls needed to make western blotting data reliable. The presentation also includes tips to save time and labor during the blotting procedure.
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ArticlesElectrophoresis/Western BlottingFeatured Stories

Revealing BRCA2 Pathways in Cancer with Bio-Rad’s V3 Western Workflow

Twenty years after the discovery of the link between BRCA gene mutations and breast cancer, researcher Ryan Jensen of the Yale Medical School is looking into the interactions between the DNA repair protein RAD51 and BRCA proteins using Bio-Rad’s V3 Western Workflow.
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ArticlesGeneral Interest

Bio-Rad’s Applications & Technologies Pages: A Valuable Resource for Scientists

Discover the wide range of applications for Bio-Rad products in diverse fields of study and learn more about our cutting-edge technologies and their underlying principles. Get protocols, troubleshooting tips, and updates on the latest developments in methods, instrumentation, and software.
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