Did you know that you can eliminate at least four steps from your qPCR gene expression workflow? That’s right.

With PrimePCR Assays and Panels, we’ve designed primers and probes for your gene expression experiments for you. By doing this, we help you eliminate multiple steps from your reverse transcription-quantitative PCR (RT-qPCR) gene expression workflow and, in the process, save you a ton of time and frustration.


Primer/Probe Sequence Determined

After you’ve identified your gene of interest, all you have to do is come to the PrimePCR website, search for your gene target, and buy the primers and/or probes you need. No need to scour the literature for a sequence that may or may not work or to design it yourself.

Primers/Probes Designed

To make your life easier, our primers and probes were designed to avoid single nucleotide polymorphisms and secondary structure in primer annealing sites. Also, they are intron-spanning when possible and compatible with up-to-date experimental conditions.

PrimePCR design features

Design Feature
Why It’s Important
Maximum transcription coverageDetects the majority of expressed mRNA transcripts for each gene of interest
Intron-spanning assay design when possibleDetects only cDNA and not contaminating gDNA
Secondary structure avoidedSecondary structure of cDNA can lead to poor efficiency of the qPCR assay
Single nucleotide polymorphisms (SNPs) avoidedSNPs in primer and probe annealing sequences can lead to poor assay efficiency
Specific sequences identifiedAvoids coamplification of nonspecific off-target sequences

Primers/Probes Tested and Validated

To keep you and, let’s face it, ultimately your PI, happy, we made sure to bench test our primers and probes for specificity, efficiency, linear dynamic range, and primer-dimers. We even used next-generation sequencing to confirm the specificity of the PCR products for every single human, mouse, and rat assay.

PrimePCR validation features

Validation Feature
Why It’s Important
7-point serial dilutionDetermines the dynamic range, R2, and efficiency of the assay
Amplification of gDNADetermines whether the assay detects contaminating gDNA
Melt curve analysisConfirms melt profile of expected qPCR product
Next-generation sequencingConfirms specificity of the assay
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