Latest Real-Time qPCR/PCR Articles

Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR
ArticlesFeatured StoriesProtocols and TipsReal-time qPCR/PCR

Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR

One-step multiplex RT-qPCR is a technique used to rapidly quantify multiple targets directly from RNA in a single reaction. But proper optimization and validation is essential for its success. These five tips will have you designing primers and probes, selecting reporters, and validating and optimizing your experiments like a pro.
READ MORE →
ROS Virus App Note Banner
ArticlesFeatured StoriesReal-time qPCR/PCR

Sensitive Codetection of Viral-Pathogen RNA and DNA
Using One-Step Multiplex RT-qPCR

RT-qPCR is a well-established method for the detection, quantification, and typing of different microbial agents. However, when dealing with the challenges of pathogen detection, not all RT-qPCR reagents are created equal. See how we used a novel one-step multiplex RT-qPCR supermix to demonstrate sensitive codetection of viral RNA and DNA targets in a multiplex setting.
READ MORE →

More Real-Time qPCR/PCR Articles