Electrophoresis/Western Blotting Articles

ArticlesElectrophoresis/Western BlottingProtocols and Tips

Resolved — Comic Series

December 11, 2019
7.4k 0
Follow along as a new starry-eyed graduate student learns the ins and outs of life as a research scientist, gaining valuable western blotting tips and tricks. Along for the ride are a seasoned postdoctoral fellow and traditional principal investigator.
READ MORE →
Electrophoresis/Western BlottingPast WebinarsWebinars

Fluorescent Immunoblots and Multiplex Analysis

September 11, 2019
215 0

Presented by: Gary F. Ross, PhD, Proteomics Field Application Scientist

View on demand

Learn best practices for fluorescent western blotting, including multiplex analysis and image acquisition.

READ MORE →

More Electrophoresis/Western Blotting Articles

Advancements in Western Blotting Technology: A Western Workflow That Delivers More Precise, Accurate, and Reproducible Results
433 April 10, 2019
10 Tips for Western Blot Detection of Phosphorylation Events
10 Tips for Western Blot Detection of Phosphorylation Events
826 February 13, 2019
See the Signal — Illuminating the Pathway to Confident Western Blot Detection of Phosphorylated Proteins
345 November 14, 2018
Six Tips to Improve Your Co-IP Results
Six Tips to Improve Your Co-IP Results
1.1k October 9, 2018
Western Blot Detection of Proteins Present in Tissue Lysates
10 Tips for Western Blot Detection of Proteins Present in Tissue Lysates
1.3k August 15, 2018
Defining the New Normal in Quantitative Western Blot Data
6.5k August 15, 2018
Publishing Meaningful Antibody Data
Finding a Good Antibody:
Part III – Publish Meaningful Data
345 July 10, 2018
Antibody Validation for Your Application
Finding a Good Antibody:
Part II — Antibody Validation
390 June 12, 2018
Best Practices for the Best Western Blots
280 June 12, 2018
Finding a Good Antibody: Part I — Selecting the Best Antibodies.
Finding a Good Antibody:
Part I — Selecting the Best Antibodies
728 May 9, 2018
The How and Why of Normalizing Your Western Blots
The How and Why of Normalizing Your Western Blots
4.6k March 14, 2018
Generating Semi-Quantitative Western Blot Data Using Bio-Rad’s Image Lab™ 6.0 Software
484 February 13, 2018
Tips to Make Fluorophore Picking Easier
995 October 11, 2017
Identify Major Immune Cell Types by Their CD Marker Expression
871 June 15, 2017
Validating Antibodies — the Good, the Bad, and the Necessary
751 May 9, 2017
Fluorescent Western Blotting: Helpful Tips for Optimizing Your Protocol
3.2k February 15, 2017
The Fluorescent Way: Why Start Fluorescent Western Blotting Now?
3.4k January 12, 2017
Ten Tips for Improving Western Blot Detection of Immunoprecipitation Samples
427 November 9, 2016
Six Tips for Efficient Transfer of Your Proteins
10k September 15, 2016
A Superior Method for Parallel Analysis of RNA and Protein Expression from Cell Culture Lysates
1.8k June 16, 2016
Tips for Selecting the Best Gel for Your Application
524 June 16, 2016
Western Blot Detection without Interfering IgGs: A Mere Fantasy or a Real Possibility?
2.4k April 12, 2016
How Does an Antibody Qualify To Become a PrecisionAb™ Antibody?
677 March 8, 2016
PrecisionAb™ Antibodies: Defining New Standards for Western Blotting
565 February 9, 2016
The Antibody Challenge: Bio-Rad’s Precise Solution
1.4k August 11, 2015
7 Tips for Choosing the Right Antibody for Western Blotting
887 June 9, 2015
SureBeads™ Magnetic Beads Standard Immunoprecipitation Protocol
1.1k May 11, 2015
Trends in Protein Separation and Analysis — the Advance of Stain-Free Technology
8.5k September 9, 2014
Telling the Story of Western Blots with the ChemiDoc Touch Imaging System
884 August 12, 2014
Brighter Precision Plus Protein™ Dual Color Standard — The Brightest Protein Marker Available
1.4k June 10, 2014
Bio-Rad’s FastCast™ Acrylamide Kits — It’s Time to Expect More from Your Handcast Gels
709 November 12, 2013
Biologics Analysis Workflow™ – Delivering Increased Throughput, Sensitivity, Reproducibility, and Automation for cGMP Laboratories
165 August 12, 2013
Revealing BRCA2 Pathways in Cancer with Bio-Rad’s V3 Western Workflow
149 May 22, 2013
Reliable, Streamlined 2-D Western Blot Workflow for Evaluation of Antibodies Developed for Detection of Host Cell Proteins
329 May 17, 2013
Using Bio-Rad’s PDQuest Software to Generate a Match Rate between 2-D Electrophoresis and Western Blotting
99 April 22, 2013
Bio-Rad’s 2-D Electrophoresis Workflow How-To Guide, Fourth Edition, is Now Available for Download
288 March 19, 2013
Gel Doc™ EZ Imaging System — Push-Button Imaging in Just Ten Seconds
496 January 8, 2013
TGX Stain-Free™ Precast Gels Facilitate LDL Research on Potential New Drug Targets
264 October 1, 2012
Expect More From Your Chemiluminescent Substrate: Introducing Clarity™ Western ECL Substrate
970 September 4, 2012
Trans-Blot® Turbo™ Ready-to-Assemble (RTA) Kit Enables Economical, Rapid, and Efficient Western Blot Transfers
948 September 3, 2012
PDQuest™ Software: Bite-Sized Video Tutorials Make Learning 2-D Analysis Easy
166 July 18, 2012
New Gradient Format Mini-PROTEAN® TGX Stain-Free™ Precast Gels for SDS-PAGE — Streamlining Electrophoresis and Blotting Workflows
842 July 13, 2012
Image Lab™ 4.1, the Latest Software Version, Addresses the Challenges of Multiplex Analysis and Protein Normalization
7.8k June 13, 2012
Bio-Rad’s New 4x Laemmli Sample Buffer for SDS-PAGE
5.7k June 12, 2012
Novel V3 Western Workflow™ Revolutionizes Protein Research
605 May 10, 2012
Around the World with the ChemiDoc™ MP Imaging System
482 February 8, 2012
Application of Hexapeptide Libraries for Enhanced Protein Detection in Human Cellular Lysates
657 January 30, 2012
Transitioning from Chemiluminescent to Multiplex Fluorescent Blotting: Things to Consider
345 January 26, 2012
Using the new PROTEAN® i12™ IEF System to Achieve Highly Reproducible Data and Extended Separation Over Multiple pH Ranges in a Single Run
156 November 21, 2011
Use of the PROTEAN® i12™ IEF System for In-Gel Peptide Fractionation Prior to LC-MS and Comparison with Off-Gel Fractionation
484 November 18, 2011
TGX Stain-Free™ Precast Gels
1.6k November 16, 2011
ChemiDoc™ MP Imaging System
846 October 5, 2011
PROTEAN® i12™ IEF System
390 October 4, 2011
PROTEAN® i12™ IEF System: Independent Voltage and Current Control Enables Optimization of First-Dimension IEF Conditions
395 October 3, 2011
Immun-Blot® LF (Low Fluorescence) PVDF Membranes
742 October 3, 2011
Using Precision Plus Protein™ WesternC™ Standards to Validate Major Steps in the Western Blotting Workflow
2.4k June 13, 2011
Gel Doc™ EZ Imaging System – A Tale of Speed and Simplicity in Two Cities
261 June 13, 2011
Simplicity in Imaging: From Conception to Launch
783 June 13, 2011
Efficient Electrophoresis: Aiding Advances in Cardiomiopathy Research
227 June 13, 2011
Protein Quantitation Assays — Frequently Asked Questions
1.8k June 13, 2011
Using the Precision Plus Protein™ WesternC™ Standard as a QC Tool at Key Steps in Your Electrophoresis and Blotting Workflow
435 June 13, 2011