Latest Real-Time qPCR/PCR Articles
Comprehensive and effective gene expression studies evaluate both transcriptional and translational regulation. A streamlined protocol was developed for the parallel analysis of mRNA and protein levels using a single lysate produced from cultured cells.
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It is imperative to analyze both the transcriptional and translational profiles for a complete understanding of the functioning of genes. Here we describe a simple protocol that enables parallel analysis of both RNA and protein from single cell culture lysates. See how this workflow could save time and money and yield more reliable data than the traditional protocols used.