Chromatography | Bio-Radiations https://www.bioradiations.com A Resource for Life Science Research Fri, 30 Jan 2026 21:45:00 +0000 en-US hourly 1 https://www.bioradiations.com/wp-content/uploads/2025/10/BR-knocked-out-60x60.png Chromatography | Bio-Radiations https://www.bioradiations.com 32 32 Each month, join Kashef Qaadri, a biologist turned bioinformatician, as he interviews guests exploring real-world research informatics challenges through use-cases, providing insights and strategies for integrating and analyzing complex data that are driving BioPharma R&D. Bio-Rad Laboratories, Inc. false Bio-Rad Laboratories, Inc. lsgmarcomapps@gmail.com Copyright © 2019 Bio-Rad Laboratories, Inc. All rights reserved. Copyright © 2019 Bio-Rad Laboratories, Inc. All rights reserved. podcast Each month, join Kashef Qaadri, a biologist turned bioinformatician, as he interviews guests exploring real-world research informatics challenges through use-cases, providing insights and strategies for integrating and analyzing complex data that are d... Chromatography | Bio-Radiations https://www.bioradiations.com/wp-content/uploads/powerpress/bioradio_podsq_1400x1400-400.jpg https://www.bioradiations.com/chromatography/ Monoclonal Antibody Purification: 
A Two-Step Approach Using a Strong AEX-HIC Mixed-Mode Resin https://www.bioradiations.com/monoclonal-antibody-purification-a-two-step-approach-using-a-strong-aex-hic-mixed-mode-resin/ Fri, 30 Jan 2026 21:45:00 +0000 https://www.bioradiations.com/?p=13028 Monoclonal antibodies (mAbs) increasingly comprise a large portion of the product pipeline of major biopharmaceutical companies. Challenges in mAb purification include reducing production cost, developing robust processes for both product purity and viral clearance, and integrating upstream ...

The post Monoclonal Antibody Purification: 
A Two-Step Approach Using a Strong AEX-HIC Mixed-Mode Resin first appeared on Bio-Radiations.]]> A Gentler Workflow: Fc-Fusion Protein Purification with Mixed-Mode and Anion Exchange Chromatography https://www.bioradiations.com/a-gentler-workflow-fc-fusion-protein-purification-with-mixed-mode-and-anion-exchange-chromatography/ Mon, 26 Jan 2026 18:42:38 +0000 https://www.bioradiations.com/?p=13020 Traditional Protein A affinity chromatography is the gold standard for Fc-fusion protein purification, but protein denaturation and degradation due to low-pH elution are limitations. This webcast presents a proof-of-concept study demonstrating the use of ...

The post A Gentler Workflow: Fc-Fusion Protein Purification with Mixed-Mode and Anion Exchange Chromatography first appeared on Bio-Radiations.]]> Evaluation of A New Multimodal Anion Exchange Resin for Emerging Biotherapeutic Process Challenges https://www.bioradiations.com/evaluation-of-a-new-multimodal-anion-exchange-resin-for-emerging-biotherapeutic-process-challenges1125/ Tue, 11 Nov 2025 17:54:04 +0000 https://www.bioradiations.com/?p=12914 Multimodal chromatography has a history of providing value for biopharmaceutical separations. Ligands that enable binding based on both charge and hydrophobic interactions provide more opportunity for effective separation of multiple ...

The post Evaluation of A New Multimodal Anion Exchange Resin for Emerging Biotherapeutic Process Challenges first appeared on Bio-Radiations.]]> As Easy as 1, 2, 3: Achieving Purification Success with a New Two-Mode Chromatography Resin https://www.bioradiations.com/purification-success-with-new-weak-aex-hic-resin-525/ Tue, 13 May 2025 18:21:43 +0000 https://www.bioradiations.com/?p=12778 A common misconception is that stronger is always better. However, Nuvia wPrime 2A Media, a weak anion exchange hydrophobic interaction (AEX-HIC) mixed-mode chromatography resin, has won over purification scientists. Read about diverse use cases for Nuvia wPrime 2A Media and see the data for yourself.

The post As Easy as 1, 2, 3: Achieving Purification Success with a New Two-Mode Chromatography Resin first appeared on Bio-Radiations.]]> Bio-Rad’s Downstream Process Development Support and Services https://www.bioradiations.com/bio-rads-downstream-process-support-services-525/ Tue, 13 May 2025 18:21:00 +0000 https://www.bioradiations.com/?p=12760 Bio-Rad’s Chromatography Support Scientist, Artur Stanczak, discusses the company’s comprehensive downstream process development services. From method development to troubleshooting, Bio-Rad provides specialized support to help customers overcome bioprocessing challenges. Access tools, case studies, and expert guidance to optimize processes and advance biomanufacturing efforts in this informative video.

The post Bio-Rad’s Downstream Process Development Support and Services first appeared on Bio-Radiations.]]> Development of an IEX Purification Process for Lentiviral Vectors https://www.bioradiations.com/iex-purification-process-for-lentiviral-vectors-225/ Tue, 11 Feb 2025 18:03:46 +0000 https://www.bioradiations.com/?p=12691 In recent years, gene therapy treatments have increased significantly, requiring more efficient processes to purify and deliver these therapeutics. Lentiviral vectors (LVVs) are a type of retrovirus and are an effective tool for gene delivery. In this webinar, data are shown for a high-throughput screening strategy that was employed for LVV purification, which provided high yield as well as acceptable host cell protein (HCP) clearance.

The post Development of an IEX Purification Process for Lentiviral Vectors first appeared on Bio-Radiations.]]> Evaluation of a New Multimodal Anion Exchange Resin for Emerging Biotherapeutic Process Challenges https://www.bioradiations.com/nuvia-wprime-2a-resin-has-mmaex-functionality-with-a-weak-anion-exchanger-see-data-showing-improved-mab-and-bsab-purification-compared-to-traditional-resins-225/ Tue, 11 Feb 2025 17:59:48 +0000 https://www.bioradiations.com/?p=12687 Nuvia wPrime 2A Resin has MMAEX functionality with a weak anion exchanger. See data showing improved mAb and bsAb purification compared to traditional resins.

The post Evaluation of a New Multimodal Anion Exchange Resin for Emerging Biotherapeutic Process Challenges first appeared on Bio-Radiations.]]> 6 Essential Considerations for Choosing Your Chromatography Resin https://www.bioradiations.com/6-tips-for-the-right-chromatography-resin-1124/ Tue, 12 Nov 2024 18:35:10 +0000 https://www.bioradiations.com/?p=12672 Choosing the right chromatography resin is a complex and critical decision that can significantly impact the success of your downstream purification workflow, whether for preparative or analytical applications. To make an informed choice, you must consider the scientific principles of the process, practical implications for your specific application, and attributes of the manufacturer.

The post 6 Essential Considerations for Choosing Your Chromatography Resin first appeared on Bio-Radiations.]]> Coming Together Is the Beginning: Working Together Is the Success https://www.bioradiations.com/working-together-is-the-success-1124/ Tue, 12 Nov 2024 17:35:27 +0000 https://www.bioradiations.com/?p=12652 Follow the story of Evitria as it grows from a small Chinese hamster ovary (CHO) cell supernatant supplier to a global therapeutic antibody production company serving clients across the globe. Bio-Rad has partnered with Evitria at every stage of this remarkable journey and looks forward to supporting its continued success.

The post Coming Together Is the Beginning: Working Together Is the Success first appeared on Bio-Radiations.]]> Developing Efficient Downstream Purification Processes for Bispecific Antibodies https://www.bioradiations.com/downstream-purification-of-bispecific-antibodies-1124/ Tue, 12 Nov 2024 17:28:14 +0000 https://www.bioradiations.com/?p=12649 Modern chromatographic resins enable new strategies for downstream processing of bispecific antibodies. Watch this webinar to see case studies and workflows.

The post Developing Efficient Downstream Purification Processes for Bispecific Antibodies first appeared on Bio-Radiations.]]> Enhancing Biotherapeutic Purification Workflows with Mixed-Mode Chromatography https://www.bioradiations.com/biotherapeutic-purification-with-mixed-mode-chromatography-824/ Tue, 13 Aug 2024 21:41:29 +0000 https://www.bioradiations.com/?p=12615 As biotherapeutic modalities have diversified and production processes become more advanced, an evolution in purification strategies is also needed. Modern biotherapeutic purification must be capable of removing multiple types of impurities without compromising yield or quality, in a cost-effective manner. To meet these needs, many are turning to mixed-mode chromatography.

The post Enhancing Biotherapeutic Purification Workflows with Mixed-Mode Chromatography first appeared on Bio-Radiations.]]> Strategies to Address Large Molecule Purification Challenges https://www.bioradiations.com/strategies-to-address-large-molecule-purification-challenges-824/ Tue, 13 Aug 2024 19:44:48 +0000 https://www.bioradiations.com/?p=12599 Large molecule purification presents significant challenges due to their considerable size. For instance, Immunoglobulin M (IgM) molecules, ranging from 900 to 1,000 kD, play pivotal roles in mammalian immune responses and find extensive applications in diagnostics and therapeutics. Purification of such molecules is particularly intricate owing to several factors including their weak Protein A binding sites, limited dynamic binding capacity, and slow diffusion rates.

The post Strategies to Address Large Molecule Purification Challenges first appeared on Bio-Radiations.]]> CHT Chromatography Media: 5 Essential Facts https://www.bioradiations.com/cht-chromatography-media-5-essential-facts-624/ Tue, 11 Jun 2024 18:47:14 +0000 https://www.bioradiations.com/?p=12563 For over 30 years, CHT Ceramic Hydroxyapatite Chromatography Media has been a trusted, proven purification solution for many biopharmaceutical manufacturing processes. CHT Media provides exceptional removal of impurities when other chromatography resins fall short.
In this webcast, explore the essential facts and benefits of this chromatography technology.

The post CHT Chromatography Media: 5 Essential Facts first appeared on Bio-Radiations.]]> Nature’s Lipid Nanoparticles: Challenges and Opportunities in Isolation and Analysis of Extracellular Vesicles https://www.bioradiations.com/extracellular-vesicle-isolation-and-analysis-624/ Tue, 11 Jun 2024 18:39:49 +0000 https://www.bioradiations.com/?p=12556 In nature, mammalian extracellular vesicles (EVs) are vital for intercellular communication, immune responses, and cell removal. EVs also hold promise in diagnostics, therapeutics, and vaccine delivery due to their abundance and natural role as carriers. Explore how overcoming challenges in EV purification and precise RNA analysis methods can help unlock their potential applications.

The post Nature’s Lipid Nanoparticles: Challenges and Opportunities in Isolation and Analysis of Extracellular Vesicles first appeared on Bio-Radiations.]]> Scalable AAV Vector Purification Workflow Using AEX and Mixed-Mode Chromatography https://www.bioradiations.com/scalable-aav-vector-purification-workflow-224/ Mon, 12 Feb 2024 18:12:35 +0000 https://www.bioradiations.com/?p=12527 Anion exchange and mixed-mode chromatography offer efficient and flexible AAV purification. Learn new strategies for optimizing viral vector purification.

The post Scalable AAV Vector Purification Workflow Using AEX and Mixed-Mode Chromatography first appeared on Bio-Radiations.]]> Automated Multicolumn Monoclonal Antibody Purification https://www.bioradiations.com/automated-monoclonal-antibody-purification-1123/ Tue, 14 Nov 2023 19:56:23 +0000 https://www.bioradiations.com/?p=12487 The current gold standard in monoclonal antibody purification for most researchers is affinity purification with Protein A or G followed by size exclusion chromatography (SEC). Although robust and highly effective, this workflow does have critical pain points and inefficiencies, leaving researchers to feel that they must oversee every step of their workflow, not leaving their chromatography system unattended for more than a few minutes.

The post Automated Multicolumn Monoclonal Antibody Purification first appeared on Bio-Radiations.]]> mAb Purification Process Development Using Mixed-Mode Resins — The DOE Approach https://www.bioradiations.com/mab-purification-process-development-1123/ Tue, 14 Nov 2023 19:36:16 +0000 https://www.bioradiations.com/?p=12476 In this case study, process chromatography application scientist Artur Stanczak presents a two-step mAb purification workflow, comparing multimodal resin alternatives used for the polishing step that occurs after protein A capture. The design of experiment (DOE) data presented characterizes the behavior of multiple mAbs with two different mixed-mode resins. The importance of scale, prior knowledge, and the stage of development will also be discussed. The results of both mixed-mode resin studies and the different DOE approaches demonstrate the ability to achieve high-purity products.

The post mAb Purification Process Development Using Mixed-Mode Resins — The DOE Approach first appeared on Bio-Radiations.]]> Unraveling the Mysteries of Molecular Machines https://www.bioradiations.com/unraveling-the-mysteries-of-molecular-machines-823/ Tue, 08 Aug 2023 19:11:03 +0000 https://www.bioradiations.com/?p=12422 Seychelle M. Vos, PhD, has a passion for science that was ignited at a young age while exploring her parents’ garden and the natural world around her. Now she studies some of the biggest questions in genome biology: how DNA structure and chromatin influence protein transcription. The molecular machines that physically couple transcription and DNA structure by changing the position of nucleosomes, interfacing with chromatin, and compressing DNA into loops, are the focus of Vos’ research.

The post Unraveling the Mysteries of Molecular Machines first appeared on Bio-Radiations.]]> Efficient and Rapid Purification of E. coli–Expressed Toxin Recombinant Protein Fragments https://www.bioradiations.com/efficient-purification-of-recombinant-toxin-823/ Mon, 07 Aug 2023 20:22:55 +0000 https://www.bioradiations.com/?p=12383 Tetanus toxoid (TT) is a common carrier protein for conjugate vaccines, but its production is faced with significant challenges. Learn about a purification process that yields over 98% pure target protein in this video.

The post Efficient and Rapid Purification of E. coli–Expressed Toxin Recombinant Protein Fragments first appeared on Bio-Radiations.]]> Next-Generation Chromatography Teaching Labs Set Students Up for Success https://www.bioradiations.com/next-generation-chromatography-teaching-labs-523/ Wed, 10 May 2023 08:21:16 +0000 https://www.bioradiations.com/?p=12360 Dominique Ingato, PhD, is a faculty member at MiraCosta College’s Department of Biotechnology. As one of only fifteen community colleges in California to offer a bachelor’s degree, MiraCosta College’s bachelor’s program in biomanufacturing is breaking ground. As part of this program, Ingato runs a next-generation chromatography (NGC) teaching lab, where students receive practical, hands-on training using state-of-the-art instruments, such as the NGC Chromatography System.

The post Next-Generation Chromatography Teaching Labs Set Students Up for Success first appeared on Bio-Radiations.]]> Assessing Viral Clearance in Early-Phase Process Development https://www.bioradiations.com/assess-viral-clearance-in-process-development-523/ Wed, 10 May 2023 07:59:07 +0000 https://www.bioradiations.com/?p=12348 Viral clearance studies are part of a multifaceted approach to ensure biopharmaceutical product safety. To prevent costly changes to a manufacturing process, it is important to assess each operation unit for its efficiency early on during downstream process development. See how a design of experiments (DOE) approach was utilized in this case study to investigate the effect of buffer pH and conductivity on the removal of impurities by an anion exchange (AEX) mixed-mode chromatography resin.

The post Assessing Viral Clearance in Early-Phase Process Development first appeared on Bio-Radiations.]]> Scalable Purification Strategies for Viral Vectors and Vaccines https://www.bioradiations.com/scalable-purification-for-viral-vectors-and-vaccines-223/ Tue, 14 Feb 2023 21:05:00 +0000 https://www.bioradiations.com/?p=12284 Large-scale downstream processing of viruses can face a range of obstacles different from those of many biotherapeutics. These challenges mostly arise from the size and complexity of the virus, which can affect product purity and recovery. Improvements of purification tools are necessary to overcome these challenges and must be engineered for easy scalability to meet manufacturing demands. In this video, Dr. Mark Snyder discusses common difficulties when developing purification strategies for vaccine production and gene therapy.

The post Scalable Purification Strategies for Viral Vectors and Vaccines first appeared on Bio-Radiations.]]> Solving Protein Purification Challenges https://www.bioradiations.com/solving-protein-purification-challenges-1022/ Tue, 11 Oct 2022 19:06:34 +0000 https://www.bioradiations.com/?p=12225 Carter Mitchell, PhD, is a protein problem solver. As Chief Science Officer at Kemp Proteins, he helps academic and industry researchers develop a range of protein therapeutics, diagnostics, and molecular tools in milligram to gram quantities with bioprocess scale in mind. In this interview, we discussed how Kemp Proteins ensures delivery of high-quality processes and protein-based products to their clients, even when working with difficult proteins.

The post Solving Protein Purification Challenges first appeared on Bio-Radiations.]]> What You Need to Know About Packing Resins at Process Scale https://www.bioradiations.com/packing-resins-at-process-scale-1022/ Tue, 11 Oct 2022 18:33:11 +0000 https://www.bioradiations.com/?p=12208 Learn best practices and guidelines for successfully packing different types of chromatography resins in this video webinar. This includes incompressible resins, like CHT Ceramic Hydroxyapatite Media, as well as traditional compressible resins. In general, parameters such as compression factor, settling rate, and attributes of the base matrix need to be considered when packing resins at process scale. Packing methods can be adapted to obtain simple, highly reproducible packing. Get tips here.

The post What You Need to Know About Packing Resins at Process Scale first appeared on Bio-Radiations.]]> Single-Step Influenza Virus Purification Using an Anion Exchange Resin https://www.bioradiations.com/single-step-influenza-virus-purification-using-an-anion-exchange-resin-722/ Wed, 13 Jul 2022 04:17:06 +0000 https://www.bioradiations.com/?p=12172 A new and simplified anion exchange chromatographic process for the purification of both live and inactivated cell grown H1N1 influenza viruses was established with a recently developed anion exchanger. The resin is designed with an optimized surface extender and pore size for superior accessibility and large biomolecule binding capacity, aiming to overcome common drawbacks of existing ion exchangers for virus purification.

The post Single-Step Influenza Virus Purification Using an Anion Exchange Resin first appeared on Bio-Radiations.]]> A Mixed-Mode Approach — An Alternative to a Three-Step mAb Purification Process https://www.bioradiations.com/a-mixed-mode-approach-an-alternative-to-a-three-step-mab-purification-process-722/ Wed, 13 Jul 2022 01:41:32 +0000 https://www.bioradiations.com/?p=12148 Monoclonal antibody (mAb) purification methods often involve the use of resin-based chromatography. In this video, we compare a three-step mAb purification workflow, which uses Nuvia Ion Exchange Resins post–Protein A capture, to a two-step purification workflow, which includes a Protein A capture step followed by a mixed-mode chromatography step using Nuvia aPrime 4A Resin, a hydrophobic anion exchange resin. We present an additional two-step purification approach using Protein A resin followed by a calcium affinity cation exchange media, CHT Ceramic Hydroxyapatite XT Media. The results of both studies demonstrate that a high purity product can be attained using one fewer unit operation than traditional workflows.

The post A Mixed-Mode Approach — An Alternative to a Three-Step mAb Purification Process first appeared on Bio-Radiations.]]> Powering Antibody Production and Purification with the NGC Chromatography System https://www.bioradiations.com/antibody-production-with-the-ngc-system-422/ Tue, 12 Apr 2022 16:22:05 +0000 https://www.bioradiations.com/?p=12068 For this article, we spoke with Vera Dreyfuss of evitria AG, an antibody expression services provider based in Switzerland, about the role that the NGC Chromatography System has played in powering the growth and success of the company.

The post Powering Antibody Production and Purification with the NGC Chromatography System first appeared on Bio-Radiations.]]> Manufacturing a Recombinant Retrovirus — Downstream Purification Process Development https://www.bioradiations.com/manufacturing-a-recombinant-retrovirus-422/ Tue, 12 Apr 2022 16:19:30 +0000 https://www.bioradiations.com/?p=12079 Enveloped virus and VLP purification can be difficult. Learn about key factors and a cGMP-compatible, low cost, scalable process for retrovirus vector capture.

The post Manufacturing a Recombinant Retrovirus — Downstream Purification Process Development first appeared on Bio-Radiations.]]> The Process of Resin Screening https://www.bioradiations.com/the-process-of-resin-screening-122/ Tue, 11 Jan 2022 08:55:48 +0000 https://www.bioradiations.com/?p=12059 Process development scientists, faced with an increasing number of proteins or complex biomolecules, continuously search for robust purification methods that can be rapidly scaled for manufacturing. What are the optimal purification conditions for high throughput and yield? Which resin or resin combination best purifies the desired protein? How can an efficient, yet cost-effective process be established? The answer: use a systematic screening approach. Read on to learn more.

The post The Process of Resin Screening first appeared on Bio-Radiations.]]> A Scalable Immobilized Metal Affinity Chromatography Media for Process Purification https://www.bioradiations.com/a-scalable-immobilized-metal-affinity-chromatography-media-for-process-purification-122/ Tue, 11 Jan 2022 08:38:19 +0000 https://www.bioradiations.com/?p=12042 Downstream process purification of proteins requires a resin with optimized bead size for ideal pressure/flow properties and decent dynamic binding capacity (DBC) that provides production efficiencies and good process economics. Nuvia IMAC Resin provides the mechanical strength, pore structures, ligand density, and particle size distribution required for an operational run at 300 cm/hr with a DBC of 40 mg/ml at 2 bar column backpressure. Protein purification can efficiently be scaled up from milligrams in the lab to kilograms in bioprocess manufacturing. The chemical stability of Nuvia IMAC Resins ensure efficient column regeneration, reproducibility between purification runs, extended column lifetime, and product purity. Watch our video to learn how Nuvia IMAC Resins overcome the challenges associated with process purification of proteins using metal affinity chromatography.

The post A Scalable Immobilized Metal Affinity Chromatography Media for Process Purification first appeared on Bio-Radiations.]]> Bioprocessing Recombinant Proteins https://www.bioradiations.com/bioprocessing-recombinant-proteins-921/ Tue, 14 Sep 2021 16:32:46 +0000 https://www.bioradiations.com/?p=11927 Infectious diseases and cancers are among the main causes of human deaths worldwide. One successful avenue found to treat these diseases is the large-scale production of therapeutic proteins. As a result, the demand for protein expression hosts and accompanying advanced bioprocessing technologies has increased. Read about a workflow for efficient purification of a low-expressing recombinant protein using mixed-mode resins.

The post Bioprocessing Recombinant Proteins first appeared on Bio-Radiations.]]> Two-Step Purification of Low-Expressing Recombinant Exoprotein A Using Mixed-Mode Chromatography https://www.bioradiations.com/two-step-purification-of-low-expressing-recombinant-exoprotein-a-using-mixed-mode-chromatography-921/ Tue, 14 Sep 2021 16:32:01 +0000 https://www.bioradiations.com/?p=11934 Purifying proteins that are not highly expressed can be challenging. Watch this video to learn about a case study demonstrating an efficient workflow for two-step purification of a low-expressing recombinant protein using mixed-mode chromatography.

The post Two-Step Purification of Low-Expressing Recombinant Exoprotein A Using Mixed-Mode Chromatography first appeared on Bio-Radiations.]]> Single-Step Column Purification for H1N1 VirusUsing Nuvia HP-Q AEX Resin https://www.bioradiations.com/single-step-column-purification-for-h1n1-virususing-nuvia-hp-q-aex-resin/ Wed, 09 Jun 2021 00:01:27 +0000 https://www.bioradiations.com/?p=11869 Learn how a team developed a single-step workflow for column purification of the Influenza A H1N1 subtype using the recently developed Nuvia HP-Q Anion Exchange Resin. This protocol is scalable, efficient, and produced good yields with high purity.

The post Single-Step Column Purification for H1N1 Virus
Using Nuvia HP-Q AEX Resin first appeared on Bio-Radiations.]]> Purification of Untagged Proteins Made Attainable for Any Researcher https://www.bioradiations.com/purification-of-untagged-proteins-made-attainable-321/ Mon, 08 Mar 2021 21:45:42 +0000 https://www.bioradiations.com/?p=11801 High purity protein is a common requirement for biochemical and structural studies. A common approach is to recombinantly express an affinity-tagged version of the protein of interest. However, this is not always a viable option. Here we showcase how the NGC Chromatography System and prepacked chromatography columns can be utilized to develop purification workflows for untagged proteins.

The post Purification of Untagged Proteins Made Attainable for Any Researcher first appeared on Bio-Radiations.]]> Automate Your Antibody Purification Workflow at Preparative and Analytical Scale https://www.bioradiations.com/automate-your-antibody-purification-workflow-at-preparative-and-analytical-scale/ Wed, 20 Jan 2021 20:15:19 +0000 https://www.bioradiations.com/?p=11772 Presented by: Katie Schaefer, PhD, Global Product Manager, Bio-Rad
Peter Winship, PhD, Technical Product Manager, Teledyne Cetac Technologies

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Purification is a multistep process toward confirmation of a target protein’s purity. In this webinar you will learn how to automate your protein purification workflow by following the creation of a hypothetical monoclonal antibody (mAb). Dr. Schaefer and Dr. Winship expertly demonstrate how the NGC Chromatography System and the AEX-500 series Autosampler work together to maximize screening capability as well as rapidly achieve therapeutic development objectives.

The post Automate Your Antibody Purification Workflow at Preparative and Analytical Scale first appeared on Bio-Radiations.]]> Are biofuels good for the planet? https://www.bioradiations.com/are-biofuels-good-for-the-planet/ Tue, 08 Dec 2020 18:51:02 +0000 https://www.bioradiations.com/?p=11748 Will the trend toward using biofuels benefit the earth? Listen in as our Chromies team discusses different types of biofuels and how chromatography plays a role in developing mass produced and energy efficient biofuels.

The post Are biofuels good for the planet? first appeared on Bio-Radiations.]]> Serving your Downstream Purification Needs https://www.bioradiations.com/serving-your-downstream-purification-needs/ Tue, 08 Dec 2020 18:49:28 +0000 https://www.bioradiations.com/?p=11741 The Process Chromatography Team at Bio-Rad offers a variety of resources to address scientists’ needs during the downstream purification process. Visit our robust online library, which contains application notes, tutorials, webinars, and more, or contact us to speak with a specialist or request a customized seminar.

The post Serving your Downstream Purification Needs first appeared on Bio-Radiations.]]> How to Make a Successful Vaccine https://www.bioradiations.com/how-to-make-a-successful-vaccine/ Wed, 09 Sep 2020 21:05:12 +0000 https://www.bioradiations.com/?p=11636 As phase 3 clinical trials of investigational vaccines for COVID-19 begin in the U.S., you may be wondering what it takes to make an effective vaccine? In case you missed it, check out our Hanging with My Chromies podcast, episodes 6 and 10 on vaccine development and viral spread, and learn about the role chromatography plays in making a successful vaccine.

The post How to Make a Successful Vaccine first appeared on Bio-Radiations.]]> Analyzing Carbohydrates https://www.bioradiations.com/analyzing-carbohydrates/ Wed, 10 Jun 2020 17:32:13 +0000 https://www.bioradiations.com/?p=11560 Carbohydrates are among the most important components of foods, providing energy as well as flavor, texture, and other physical properties. Identification and quantification of carbohydrates in food is commonly performed using high-performance liquid chromatography. Bio-Rad Aminex Ion Exchange Resins have become the industry standard for carbohydrate and organic acid analysis.

The post Analyzing Carbohydrates first appeared on Bio-Radiations.]]> Biosimilar Purification with Ion Exchange and Mixed-Mode Resins https://www.bioradiations.com/biosimilar-purification-with-ion-exchange-and-mixed-mode-resins/ Wed, 12 Feb 2020 20:43:12 +0000 https://www.bioradiations.com/?p=11478 Presented by: Randy Drevland, PhD, Senior Applications Scientist

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Dr. Drevland presents a viable alternative workflow for the development of a HUMIRA-like biosimilar using IEX and mixed-mode resins.

The post Biosimilar Purification with Ion Exchange and Mixed-Mode Resins first appeared on Bio-Radiations.]]> Hanging with My Chromies Podcast Series https://www.bioradiations.com/hanging-with-my-chromies-podcast-series/ Thu, 12 Dec 2019 00:04:21 +0000 https://www.bioradiations.com/?p=10437 The Hanging with My Chromies podcast series is focused on the many surprising applications of chromatography and the stories surrounding them. Join us every month to see what you can learn.

The post Hanging with My Chromies Podcast Series first appeared on Bio-Radiations.]]> Maximizing the Efficiency of Production Workflows with NGC Chromatography Systems https://www.bioradiations.com/maximizing-the-efficiency-of-production-workflows-with-ngc-chromatography-systems/ Thu, 12 Dec 2019 00:00:51 +0000 https://www.bioradiations.com/?p=11403 See how an antibody expression service provider, Evitria (Zurich, Switzerland) optimized and expanded their antibody production services using the NGC Chromatography Systems to meet the growing demands of their customers.

The post Maximizing the Efficiency of Production Workflows with NGC Chromatography Systems first appeared on Bio-Radiations.]]> Integrating Viral Clearance into Your Process Development — A Case Study Using a Mixed‑Mode Chromatography Resin https://www.bioradiations.com/integrating-viral-clearance-into-your-process-development/ Wed, 13 Nov 2019 21:18:55 +0000 https://www.bioradiations.com/?p=11366 Presented by: Akunna Iheanacho, Director of Research and Development at Texcell – North America
Xuemei He, R&D Manager, Chromatograpy Media Chemistry at Bio-Rad Laboratories

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Learn about evaluating viral clearance using a DOE approach with a mixed-mode chromatography resin.

The post Integrating Viral Clearance into Your Process Development — A Case Study Using a Mixed‑Mode Chromatography Resin first appeared on Bio-Radiations.]]> Challenging the Microbial Sanitization of a Liquid Chromatography System https://www.bioradiations.com/challenging-the-microbial-sanitization-of-a-liquid-chromatography-system/ Wed, 13 Nov 2019 21:07:31 +0000 https://www.bioradiations.com/?p=11330 When developing biologics or biotherapeutics, it is imperative that no contaminating or hazardous residual components from the organisms responsible for protein expression remain after purification. See how the NGC Chromatography System was challenged with microbes and efficiently sanitized, making it ideal for the small-scale production of biotherapeutics.

The post Challenging the Microbial Sanitization of a Liquid Chromatography System first appeared on Bio-Radiations.]]> Purification of Antibodies with Nuvia aPrime 4A Hydrophobic Anion Exchange Resin https://www.bioradiations.com/purification-of-antibodies-with-nuvia-aprime-4a-hydrophobic-anion-exchange-resin/ Tue, 13 Aug 2019 23:33:43 +0000 https://www.bioradiations.com/?p=11173 Our new hydrophobic anion exchange mixed-mode resin, Nuvia aPrime 4A, has been developed to overcome existing challenges in antibody purification. See how we purified an acidic and basic antibody through bind-elute and flow-through modes of purification, respectively, and helped to improve process productivity and economy.

The post Purification of Antibodies with Nuvia aPrime 4A Hydrophobic Anion Exchange Resin first appeared on Bio-Radiations.]]> A New AEX Mixed-Mode Chromatography Resin for High Selectivity and Recovery https://www.bioradiations.com/a-new-aex-mixed-mode-chromatography-resin-for-high-selectivity-and-recovery/ Tue, 11 Jun 2019 20:26:50 +0000 https://www.bioradiations.com/?p=10889 Presented by: Xuemei He, PhD, R&D Manager, Chromatography Media Chemistry, Bio-Rad Laboratories

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This webinar highlights the properties of Bio-Rad’s newest hydrophobic AEX chromatography resin, Nuvia aPrime 4A, which is designed to facilitate selective and reversible binding of target molecules for higher purity and recovery over traditional chromatography resins.

The post A New AEX Mixed-Mode Chromatography Resin for High Selectivity and Recovery first appeared on Bio-Radiations.]]> Discover Our Capabilities https://www.bioradiations.com/discover-our-capabilities/ Wed, 10 Apr 2019 18:16:19 +0000 https://www.bioradiations.com/?p=10698 From flow cytometry to chromatography to western blot imaging to PCR plastics, learn more about Bio-Rad and see what’s possible in your research.

The post Discover Our Capabilities first appeared on Bio-Radiations.]]> Stop Compromising between High Yield and High Purity in Downstream Purifications https://www.bioradiations.com/stop-compromising-between-high-yield-and-high-purity-in-downstream-purifications/ Tue, 12 Mar 2019 23:59:26 +0000 https://www.bioradiations.com/?p=10596 When it comes to downstream protein purifications, does it feel like you’re in a constant tug of war between high yield and high purity? With Bio-Rad’s Nuvia aPrime 4A Resin, you don’t have to choose. With both ion exchange and mixed-mode properties, see how the resin is able to deliver simultaneous purity and yield, for even difficult-to-purify proteins.

The post Stop Compromising between High Yield and High Purity in Downstream Purifications first appeared on Bio-Radiations.]]> Changing the Treatment Landscape with the Development of Biologics https://www.bioradiations.com/changing-the-treatment-landscape-with-the-development-of-biologics/ Wed, 13 Feb 2019 21:49:54 +0000 https://www.bioradiations.com/?p=10525 Biologics are transforming the healthcare landscape and bringing us closer to the realization of personalized solutions to our health problems. Learn about what biologics are and how protein purification plays a key role in their overall success as a therapeutic.

The post Changing the Treatment Landscape with the Development of Biologics first appeared on Bio-Radiations.]]> A Model System for Fast Screening of Optimal Protein Purification Conditions https://www.bioradiations.com/a-model-system-for-fast-screening-of-optimal-protein-purification-conditions/ Wed, 09 Jan 2019 18:12:36 +0000 https://www.bioradiations.com/?p=10441 See how you can efficiently reduce identification of optimal protein purification conditions from five days to just 18 hours. All you need is a DOE model and the Multivariable Scouting tool and automation abilities of the NGC Chromatography System.

The post A Model System for Fast Screening of Optimal Protein Purification Conditions first appeared on Bio-Radiations.]]> Single-Step Influenza and Dengue Virus Purification with Mixed-Mode CHT Ceramic Hydroxyapatite XT Media https://www.bioradiations.com/single-step-influenza-and-dengue-virus-purification-with-mixed-mode-cht-ceramic-hydroxyapatite-xt-media/ Tue, 09 Oct 2018 23:18:21 +0000 http://www.bioradiations.com/?p=10181 See how you can use CHT XT Mixed-Mode Media to recover over 75% of your virus in a single purification step. All this while reducing contaminating proteins by over 90% and removing over 60% of dsDNA. Get simple, fast, and reproducible viral purification with CHT XT.

The post Single-Step Influenza and Dengue Virus Purification with Mixed-Mode CHT Ceramic Hydroxyapatite XT Media first appeared on Bio-Radiations.]]> Large Biomolecule Purification with a New High-Performance Anion Exchange Resin https://www.bioradiations.com/large-biomolecule-purification-with-a-new-high-performance-anion-exchange-resin/ Tue, 09 Oct 2018 23:11:20 +0000 http://www.bioradiations.com/?p=10169 Presented by: Hana Kim, PhD, Global Product Manager, Process Chromatography
Jiali Liao, PhD, Principal Scientist, Process Chromatography

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Learn about Nuvia HP-Q Resin, a new rigid bead engineered with large pores and optimized surface extenders to deliver both high binding capacity and high recovery at high flow rates. It is ideal for large molecule purification, including plasma proteins, viruses, and VLPs.

The post Large Biomolecule Purification with a New High-Performance Anion Exchange Resin first appeared on Bio-Radiations.]]> A New Mixed-Mode Media for Downstream Purification https://www.bioradiations.com/a-new-mixed-mode-media-for-downstream-purification/ Tue, 09 Oct 2018 23:10:36 +0000 http://www.bioradiations.com/?p=10171 Presented by: Daniel Yoshikawa, PhD, Global Product Manager, Process Chromatography
Mark A. Snyder, PhD, R&D Applications Manager, Process Chromatography

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Watch this webinar to learn about a new mixed-mode chromatography media used to purify biomolecules at process scale. It delivers unique selectivity and robust, uniformly sized beads with excellent pressure-flow properties ideal for bioprocess applications.

The post A New Mixed-Mode Media for Downstream Purification first appeared on Bio-Radiations.]]> Got Purification Troubles? https://www.bioradiations.com/got-purification-troubles/ Wed, 15 Aug 2018 14:49:39 +0000 http://www.bioradiations.com/?p=10041 Use our interactive infographic to find out how your resin-of-choice fares in on-column aggregate formation during monoclonal antibody purification. And find out what you can do to minimize these on-column aggregates.

The post Got Purification Troubles? first appeared on Bio-Radiations.]]> A Resin Built Specifically for Large Biomolecule Purifications https://www.bioradiations.com/a-resin-built-specifically-for-large-biomolecule-purifications/ Tue, 10 Jul 2018 21:35:45 +0000 http://www.bioradiations.com/?p=9953 Nuvia HP-Q is a high-performance strong anion exchange resin designed for purification of large biomolecules. See how its optimized particle and pore size leads to high target recovery and high-throughput purifications.

The post A Resin Built Specifically for Large Biomolecule Purifications first appeared on Bio-Radiations.]]> Increase Yield and Reproducibility While Saving Time by Automating Recombinant Protein Purification https://www.bioradiations.com/increase-yield-and-reproducibility-while-saving-time-by-automating-recombinant-protein-purification/ Tue, 10 Jul 2018 21:00:48 +0000 http://www.bioradiations.com/?p=9937 Presented by: Philip Chapman, MSc, Senior Global Product Manager

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Learn how to quickly optimize conditions prior to automating a four-step purification of a histidine-tagged recombinant protein. Automating purification reduces a multi-day process into 12 hours while increasing yield and reproducibility.

The post Increase Yield and Reproducibility While Saving Time by Automating Recombinant Protein Purification first appeared on Bio-Radiations.]]> Improve Your Protein Purification Process Economy with CHT Ceramic Hydroxyapatite XT Media https://www.bioradiations.com/improve-your-protein-purification-process-economy-with-cht-ceramic-hydroxyapatite-xt-media/ Tue, 12 Jun 2018 22:50:52 +0000 http://www.bioradiations.com/?p=9841 CHT Ceramic Hydroxyapatite XT Media is designed for repeated use and exceptional resolution. It combines exceptional functionality with a long column life to help achieve high productivity and improved process economics. See how CHT XT can improve your protein purification.

The post Improve Your Protein Purification Process Economy with CHT Ceramic Hydroxyapatite XT Media first appeared on Bio-Radiations.]]> Introducing the NGC™ Fraction Collector for Discovery to Process-Scale Protein Purification https://www.bioradiations.com/introducing-the-ngc-fraction-collector-for-discovery-to-process-scale-protein-purification/ Tue, 13 Feb 2018 23:59:43 +0000 http://www.bioradiations.com/?p=9603 The newly launched NGC™ Fraction Collector, together with ChromLab™ 5.0 Software, have enhanced the functionality of the NGC Chromatography System to provide the flexibility and collection capacity needed for any application, from discovery to scale up. See how the NGC System can help you improve your protein purification process.

The post Introducing the NGC™ Fraction Collector for Discovery to Process-Scale Protein Purification first appeared on Bio-Radiations.]]> Minimizing On-Column Monoclonal Antibody Aggregate Formation https://www.bioradiations.com/minimizing-on-column-monoclonal-antibody-aggregate-formation/ Tue, 09 Jan 2018 23:35:24 +0000 http://www.bioradiations.com/?p=9576 The formation of aggregates is a common but highly undesirable occurrence during monoclonal antibody purification. See how on-column aggregation is dependent on the type of resin used and how one particular high-resolution cation exchange resin allows for the lowest proportion of aggregate formation.

The post Minimizing On-Column Monoclonal Antibody Aggregate Formation first appeared on Bio-Radiations.]]> Practical Experimental Design for Mixed-Mode Resins https://www.bioradiations.com/practical-experimental-design-for-mixed-mode-resins/ Wed, 11 Oct 2017 23:53:03 +0000 http://www.bioradiations.com/?p=9326 Could you be missing opportunities to make the most of your time spent on protein chromatography experiments? Learn more about how Bio-Rad uses design of experiments (DoE) and our mixed-mode resin, Nuvia™ cPrime™, to optimize process-scale protein purification.

The post Practical Experimental Design for Mixed-Mode Resins first appeared on Bio-Radiations.]]> An IMAC Resin for a Highly Productive Histidine-Tagged Protein Purification Process https://www.bioradiations.com/an-imac-resin-for-a-highly-productive-histidine-tagged-protein-purification-process/ Wed, 09 Aug 2017 18:30:59 +0000 http://www.bioradiations.com/?p=9239 One of the advancements in protein purification has been to tag the protein of interest with a short sequence that can bind to an immobilized matrix and aid purification. Bio-Rad’s new IMAC resin offers a host of advantages for the purification of histidine-tagged proteins. Learn more about this resin and see how it can enhance the efficiency of your protein purification workflow.

The post An IMAC Resin for a Highly Productive Histidine-Tagged Protein Purification Process first appeared on Bio-Radiations.]]> Modern Improvements to Classic Technologies: Innovating Electrophoresis and Chromatography Upstream of Protein Characterization to Speed Up Research https://www.bioradiations.com/modern-improvements-to-classic-technologies-innovating-electrophoresis-and-chromatography-upstream-of-protein-characterization-to-speed-up-research/ Thu, 15 Jun 2017 21:07:15 +0000 http://www.bioradiations.com/?p=9186 When testing new drug therapies, it is essential to know the identity of the protein being tested. Chromatography is a commonly used technique to separate complex biological mixtures and isolate proteins of interest prior to downstream characterization. Learn more about how automating chromatography and stain-free technology are helping to save time and effort in protein purification.

The post Modern Improvements to Classic Technologies: Innovating Electrophoresis and Chromatography Upstream of Protein Characterization to Speed Up Research first appeared on Bio-Radiations.]]> From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification https://www.bioradiations.com/from-optimization-to-automation-multidimensional-multi-d-histidine-tag-protein-purification/ Thu, 13 Oct 2016 17:39:47 +0000 http://www.bioradiations.com/?p=8840 A well-designed multidimensional (Multi-D) chromatography process, which utilizes optimized individual column methods, can help automate multistep protein purification processes. This can help you save time and increase productivity. See how you can use Multi-D chromatography to expedite the purification of polyhistidine-tagged proteins when using a typical capture, intermediate, and polish purification workflow.

The post From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification first appeared on Bio-Radiations.]]> Coupling Automated Chromatography with Faster Confirmation of Purity and Yield https://www.bioradiations.com/coupling-automated-chromatography-with-faster-confirmation-of-purity-and-yield/ Thu, 13 Oct 2016 17:36:47 +0000 http://www.bioradiations.com/?p=8833 We’ve combined the chromatography process with purity and yield assessment using stain-free gels to shorten and optimize your protein purification workflow. See how it’s done.

The post Coupling Automated Chromatography with Faster Confirmation of Purity and Yield first appeared on Bio-Radiations.]]> Attaching a Dynaloop to the NGC™ Chromatography System https://www.bioradiations.com/attaching-a-dynaloop-to-the-ngc-chromatography-system/ Fri, 12 Aug 2016 04:47:07 +0000 http://www.bioradiations.com/?p=8770 This video describes the three main steps in attaching a DynaLoop Sample Loop to the NGC™ Chromatography System to enable the injection of larger sample volumes: connecting the DynaLoop Sample Loop to the NGC Chromatography System, priming the DynaLoop Sample Loop, and creating a method in ChromLab™ Software.

The post Attaching a Dynaloop to the NGC™ Chromatography System first appeared on Bio-Radiations.]]> Calibrating the pH Valve on the NGC™ Chromatography System https://www.bioradiations.com/calibrating-the-ph-valve-on-the-ngc-chromatography-system/ Fri, 12 Aug 2016 04:44:47 +0000 http://www.bioradiations.com/?p=8767 The NGC pH valve provides real-time, in-line monitoring of buffer pH. In this video, you’ll learn how to connect the pH valve to the NGC System, add it to the flow path, and calibrate the valve using buffers at two different pH values. Features of the pH valve include accessible calibration ports and easy bypass of the probe when the NGC System is being cleaned.

The post Calibrating the pH Valve on the NGC™ Chromatography System first appeared on Bio-Radiations.]]> Tips and Strategies for Successful Purification of Your Proteins https://www.bioradiations.com/tips-and-strategies-for-successful-purification-of-your-proteins/ Fri, 12 Aug 2016 04:36:16 +0000 http://www.bioradiations.com/?p=8757 A biomolecule protein purification workflow requires more than one resin. The trick is to find the combination of resins that provides the highest purity in the least number of steps and at the lowest cost. Here are some guides to select the optimal resins for the purification of monoclonal antibodies, native and recombinant proteins, Ig G and mammalian and adenovirus, and for removing aggregates and endotoxins.

The post Tips and Strategies for Successful Purification of Your Proteins first appeared on Bio-Radiations.]]> Synthesis and Purification of Recombinant Proteins — A Success Story https://www.bioradiations.com/synthesis-and-purification-of-recombinant-proteins-a-success-story/ Fri, 15 Jul 2016 21:24:43 +0000 http://www.bioradiations.com/?p=8730 Producing highly purified proteins in large quantities is a challenge and requires modification of methods and protocols to attain high efficiency. Dr Murat Kasap from Kocaeli University, Turkey, has overcome these difficulties by taking advantage of the modularity of the NGC Chromatography System and successfully synthesized and purified cardiac troponin-I protein.

The post Synthesis and Purification of Recombinant Proteins — A Success Story first appeared on Bio-Radiations.]]> Removing Air Bubbles from the NGC™ Chromatography System https://www.bioradiations.com/removing-air-bubbles-from-the-ngc-chromatography-system/ Fri, 15 Jul 2016 21:21:39 +0000 http://www.bioradiations.com/?p=8723 This video explains how to remove bubbles and/or reduce bubble formation in your NGC System. Tips to reduce bubbles include warming up your multiwave detector for 30 to 60 min before use and flowing buffer at 0.1–0.2 ml/min in the manual mode until a stable/flat baseline is achieved before starting a method. The system may also be flushed with 100% methanol or ethanol at purge speed, with columns out of line, to remove bubbles.

The post Removing Air Bubbles from the NGC™ Chromatography System first appeared on Bio-Radiations.]]> Using the Air Sensor on the NGC™ Chromatography System https://www.bioradiations.com/using-the-air-sensor-on-the-ngc-chromatography-system/ Fri, 15 Jul 2016 21:09:24 +0000 http://www.bioradiations.com/?p=8705 This video describes the functions of the Air Sensor Module and how to connect it to the NGC Chromatography System. The NGC Air Sensor senses the end of sample or buffer and prevents the introduction of air into the system, which can damage columns and/or incur the loss of precious samples. Both small and large air sensors are available to fit the tubing being used. Steps for connecting the Air Sensor Module and associated air sensors are described including how to add the air sensors into the system flow path using ChromLab™ Software and the differences in using the air sensors in manual vs. method modes.

The post Using the Air Sensor on the NGC™ Chromatography System first appeared on Bio-Radiations.]]> Purifying Difficult-to-Purify Proteins https://www.bioradiations.com/purifying-difficult-to-purify-proteins/ Thu, 16 Jun 2016 23:12:00 +0000 http://www.bioradiations.com/?p=8665 Protein purification becomes a challenging step for applications requiring high protein concentrations (such as structural biology studies). This is also true for researchers purifying integral membranes proteins and untagged proteins from native sources. Here we provide some useful tips for selecting the proper resins, optimizing conditions and developing effective strategies to tackle the challenges associated with such difficult-to-purify proteins.

The post Purifying Difficult-to-Purify Proteins first appeared on Bio-Radiations.]]> How Do You Automate a Multicolumn Protein Purification? https://www.bioradiations.com/how-do-you-automate-a-multicolumn-protein-purification/ Wed, 11 May 2016 20:37:44 +0000 http://www.bioradiations.com/?p=8618 Multicolumn purifications are tedious and the manual operations during this process introduce variabilities, affecting the success of purification and reproducibility of results. This whiteboard animation explains how an automated multi-dimensional chromatography method can make this an easier process with very high success rates.

The post How Do You Automate a Multicolumn Protein Purification? first appeared on Bio-Radiations.]]> Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison of CHT™ Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes https://www.bioradiations.com/mixed-mode-chromatography-for-mab-s-aggregate-removal-comparison-of-cht-ceramic-hydroxyapatite-capto-adhere-and-capto-adhere-impres/ Tue, 08 Mar 2016 18:26:30 +0000 http://www.bioradiations.com/?p=8507 The separation of monomers from product-related impurities is quite challenging in process separations. It requires a thorough screening and an efficient resin to get efficiency in the purification process. Review the performance data of Bio-Rad’s mixed mode CHT™ Ceramic Hydroxapatite in achieving process separation efficiency and how it compares with various Capto media.

The post Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison of CHT™ Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes first appeared on Bio-Radiations.]]> How Does the Mixed Mode Interaction of a Media Help Achieve High Selectivity and Resolution? https://www.bioradiations.com/how-does-the-mixed-mode-interaction-of-a-media-help-achieve-high-selectivity-and-resolution/ Tue, 09 Feb 2016 21:53:01 +0000 http://www.bioradiations.com/?p=8452 The best chromatography media should yield high protein purity and product recovery as well as be versatile enough to purify difficult samples. Bio-Rad’s CHT™ Ceramic Hydroxyapatite achieves this by virtue of being able to interact with the proteins through multiple modes, making the process extremely efficient. The whiteboard animation describes the mode of action of CHT.

The post How Does the Mixed Mode Interaction of a Media Help Achieve High Selectivity and Resolution? first appeared on Bio-Radiations.]]> Chromatography: Everything You Need to Know about Your Peaks https://www.bioradiations.com/chromatography-everything-you-need-to-know-about-your-peaks/ Wed, 13 Jan 2016 01:53:29 +0000 http://www.bioradiations.com/?p=8378 What can chromatography peaks tell us? From the quality of column packing to the purity of separated proteins, a lot can be gleaned from the peaks. Here are some definitions that can help decipher what chromatography peaks can tell us.

The post Chromatography: Everything You Need to Know about Your Peaks first appeared on Bio-Radiations.]]> High-Processivity Sso7d Fusion Polymerase Improves qPCR Results https://www.bioradiations.com/high-processivity-sso7d-fusion-polymerase-improves-qpcr-results/ Tue, 08 Dec 2015 19:01:56 +0000 http://www.bioradiations.com/?p=8108 Processivity is the rate at which nucleotides are added to extend PCR products. High processivity results in a greater number of nucleotides incorporated in each polymerase binding event.

The post High-Processivity Sso7d Fusion Polymerase Improves qPCR Results first appeared on Bio-Radiations.]]> Four Tips for Developing a Sample Prep Protocol for SDS-PAGE or Chromatography https://www.bioradiations.com/four-tips-for-developing-a-sample-prep-protocol-for-sds-page-or-chromatography/ Fri, 04 Sep 2015 17:53:25 +0000 http://www.bioradiations.com/?p=7531 From making sure that your lysis buffer is compatible with your protein quantitation method to advice on when you do and don't have to worry about proteases, we share helpful tips for developing a sample preparation protocol.

The post Four Tips for Developing a Sample Prep Protocol for SDS-PAGE or Chromatography first appeared on Bio-Radiations.]]> Five Tips for Picking the Right Cell Disruption Method for Protein Analysis https://www.bioradiations.com/five-tips-for-picking-the-right-cell-disruption-method-for-protein-analysis/ Fri, 04 Sep 2015 17:51:15 +0000 http://www.bioradiations.com/?p=7529 Sonicator or freeze-thaw? Detergent or enzymatic lysis? Find out how to pick the right cell disruption method for your experiment.

The post Five Tips for Picking the Right Cell Disruption Method for Protein Analysis first appeared on Bio-Radiations.]]> Bio-Rad’s Multi-D Method Addresses Unmet Chromatography Needs https://www.bioradiations.com/bio-rads-multi-d-method-addresses-unmet-chromatography-needs/ Thu, 03 Sep 2015 00:14:06 +0000 http://www.bioradiations.com/?p=7514 Some of the frustrations in protein purification stem from unmet chromatography needs, such as automation, productivity, throughput, and reproducibility among others. Bio-Rad’s new Multi-D chromatography method addresses these and provides solutions to faster, efficient and more reliable protein purification.

The post Bio-Rad’s Multi-D Method Addresses Unmet Chromatography Needs first appeared on Bio-Radiations.]]> Automated Multi-D Chromatography on the NGC™ System https://www.bioradiations.com/automated-multi-d-chromatography-on-the-ngc-system/ Wed, 15 Jul 2015 00:01:04 +0000 http://www.bioradiations.com/?p=7253 What is Multidimensional (Multi-D) chromatography? Here is an animated explanation of how Multi-D chromatography works and what its advantages are over traditional chromatography.

The post Automated Multi-D Chromatography on the NGC™ System first appeared on Bio-Radiations.]]> Advantages of Multidimensional (Multi-D) Chromatography Using the NGC™ Chromatography System over Traditional Sequential Chromatography https://www.bioradiations.com/advantages-of-multidimensional-multi-d-chromatography-using-the-ngc-chromatography-system-over-traditional-sequential-chromatography/ Tue, 14 Jul 2015 22:47:24 +0000 http://www.bioradiations.com/?p=7212 Traditional sequential chromatography typically requires intensive hands-on user manipulation, which could hinder the reproducibility of protein purification runs. Multi-D chromatography aims to improve this process while maintaining the efficacy of the traditional workflow.

The post Advantages of Multidimensional (Multi-D) Chromatography Using the NGC™ Chromatography System over Traditional Sequential Chromatography first appeared on Bio-Radiations.]]> Tips for Keeping Your NGC™ Chromatography System in Great Shape https://www.bioradiations.com/tips-for-keeping-your-ngc-chromatography-system-in-great-shape/ Wed, 11 Feb 2015 00:19:06 +0000 http://www.bioradiations.com/?p=5248 Doing these three simple checks will protect your chromatography system from common lab hazards.

The post Tips for Keeping Your NGC™ Chromatography System in Great Shape first appeared on Bio-Radiations.]]> Tips for Using Chromatography Columns with Your NGC™ Chromatography System https://www.bioradiations.com/tips-for-using-chromatography-columns-with-your-ngc-chromatography-system/ Wed, 11 Feb 2015 00:14:47 +0000 http://www.bioradiations.com/?p=5245 Here are the methods you need to ensure optimal, long-lasting column performance.

The post Tips for Using Chromatography Columns with Your NGC™ Chromatography System first appeared on Bio-Radiations.]]> Tips for Sample Preparation with Your NGC™ Chromatography System https://www.bioradiations.com/tips-for-sample-preparation-with-your-ngc-chromatography-system/ Wed, 11 Feb 2015 00:09:47 +0000 http://www.bioradiations.com/?p=5241 Follow these steps to go from crude cell lysate to a sample that’s ready to purify.

The post Tips for Sample Preparation with Your NGC™ Chromatography System first appeared on Bio-Radiations.]]> Tips for Storing Proteins Purified on Your NGC™ Chromatography System https://www.bioradiations.com/tips-for-storing-proteins-purified-on-your-ngc-chromatography-system/ Wed, 11 Feb 2015 00:03:22 +0000 http://www.bioradiations.com/?p=5238 Get the most out of your proteins, including better stability, the best ways to freeze fractions, and more!

The post Tips for Storing Proteins Purified on Your NGC™ Chromatography System first appeared on Bio-Radiations.]]> Tips for Preparing and Using Buffers with Your NGC™ Chromatography System https://www.bioradiations.com/tips-for-preparing-and-using-buffers-with-your-ngc-chromatography-system/ Tue, 10 Feb 2015 19:28:57 +0000 http://www.bioradiations.com/?p=5219 Here are four fast and easy things that will help you maintain peak performance for your medium pressure chromatography system. Beat contamination and save your samples!

The post Tips for Preparing and Using Buffers with Your NGC™ Chromatography System first appeared on Bio-Radiations.]]> NGC System Tour and Configurator https://www.bioradiations.com/ngc-system-tour-and-configurator/ Wed, 08 Oct 2014 22:58:50 +0000 http://www.bioradiations.com/?p=719 The NGC Chromatography System introduces a new level of modularity and usability for protein purification. The modular design of the NGC System provides multiple configurations, each addressing different requirements. Use our configurator tool to build your own system to meet your specific workflow and throughput needs.

The post NGC System Tour and Configurator first appeared on Bio-Radiations.]]> Modeling Natural Killer Cell Receptors with the NGC Chromatography System https://www.bioradiations.com/modeling-natural-killer-cell-receptors-with-the-ngc-chromatography-system/ Tue, 07 Oct 2014 00:58:37 +0000 http://www.bioradiations.com/?p=738 See how researcher Petr Novák, a structural biologist in Prague, Czech Republic, has accelerated his effort to characterize complex receptor molecules by integrating the NGC chromatography system into his lab’s ...

The post Modeling Natural Killer Cell Receptors with the NGC Chromatography System first appeared on Bio-Radiations.]]> Purification Trends: How Smart is Chromatography Automation? https://www.bioradiations.com/purification-trends-how-smart-is-chromatography-automation/ Wed, 09 Jul 2014 04:56:59 +0000 http://www.bioradiations.com/?p=614 What does automated protein purification mean today? What might it look like tomorrow? Bioradiations talks to a variety of users about what they’re doing now to implement automation in their purification processes, and what possibilities they envision that will improve chromatography in the near future.

The post Purification Trends: How Smart is Chromatography Automation? first appeared on Bio-Radiations.]]> Protein Purification Workflow Development Using Bio-Rad’s NGC™ Chromatography System https://www.bioradiations.com/protein-purification-workflow-development-using-bio-rads-ngc-chromatography-system/ Tue, 13 May 2014 18:03:19 +0000 http://www.bioradiations.com/?p=1807 While using affinity tagging is the preferred method to achieve highly pure protein through recombinant expression, this approach isn’t feasible for all proteins. This tech report describes workflow optimizations using the NGC Chromatography System that allow users to achieve high purities with untagged proteins.

The post Protein Purification Workflow Development Using Bio-Rad’s NGC™ Chromatography System first appeared on Bio-Radiations.]]> Unlocking the Structures of Natural Killer Cell Receptors with the NGC™ Chromatography System https://www.bioradiations.com/unlocking-the-structures-of-natural-killer-cell-receptors-with-the-ngc-chromatography-system/ Tue, 08 Apr 2014 20:54:47 +0000 http://www.bioradiations.com/?p=1073 Structural biologist Petr Novák employs the latest techniques to model natural killer cell receptor molecules, research that may one day result in powerful therapies to treat cancer and infections. Go inside the fascinating world of structural characterization and find out how Novák has integrated the NGC™ chromatography system into his effort to visualize these molecules in vivid detail.

The post Unlocking the Structures of Natural Killer Cell Receptors with the NGC™ Chromatography System first appeared on Bio-Radiations.]]> The Chromatography Chronicles Part 5: Chromatography You Can Count On https://www.bioradiations.com/the-chromatography-chronicles-part-5-chromatography-you-can-count-on/ Wed, 13 Nov 2013 00:08:08 +0000 http://www.bioradiations.com/?p=1140 In biochromatography, every purification or separation procedure that fails because of instrument malfunction wastes precious lab resources. That’s why Bio-Rad, in designing the NGC chromatography system, found new ways to engineer reliability into the chromatography experience. Here’s how they did it.

The post The Chromatography Chronicles Part 5: Chromatography You Can Count On first appeared on Bio-Radiations.]]> Stand Up Against Downtime https://www.bioradiations.com/stand-up-against-downtime/ Mon, 11 Nov 2013 20:00:17 +0000 http://www.bioradiations.com/?p=2168 Facing adversity with humor, some chromatographers are drawing on the healing power of laughter to deal with the frustrations of chromatography systems that just won’t work.

The post Stand Up Against Downtime first appeared on Bio-Radiations.]]> Learning Curve Blues https://www.bioradiations.com/learning-curve-blues/ Tue, 08 Oct 2013 19:00:18 +0000 http://www.bioradiations.com/?p=2181 This researcher sings a tune many will recognize from their own frustrations with overly complex chromatography instruments. The NGC™ chromatography system offers a simple, user-friendly interface so even students with little experience will beat the “learning curve blues.”

The post Learning Curve Blues first appeared on Bio-Radiations.]]> The Chromatography Chronicles Part 4: Chromatography’s Intuitive Side https://www.bioradiations.com/the-chromatography-chronicles-part-4-chromatographys-intuitive-side/ Wed, 02 Oct 2013 22:24:10 +0000 http://www.bioradiations.com/?p=1132 Chromatography instruments have a bad reputation in terms of their complexity and difficulty of use. In response, Bio-Rad’s designers and engineers challenged themselves to make the new NGC™ chromatography system as simple and intuitive-to-use as possible. Learn about the new easier-to-use instrument interface they created as a result.

The post The Chromatography Chronicles Part 4: Chromatography’s Intuitive Side first appeared on Bio-Radiations.]]> Bench Buzz – Protein Purification Product Testers https://www.bioradiations.com/bench-buzz-protein-purification-product-testers/ Tue, 10 Sep 2013 19:00:32 +0000 http://www.bioradiations.com/?p=2205 Meet Janna and Tomas. After hearing competing claims about the scalability of next-generation chromatography systems, this dynamic duo took it upon themselves to discover the truth. Now let's face it, when you think of protein purification you don't usually think of good times. But somehow Janna and Tomas manage to keep the mood light even as they put the systems to the test by simulating changing protein purification needs. Their conclusion? Vote Yes for Better Chromatography. Vote Yes for Bio-Rad.

The post Bench Buzz – Protein Purification Product Testers first appeared on Bio-Radiations.]]> The Chromatography Chronicles Part 3: Adding, Swapping, and Scaling — Better Chromatography through Modular Design https://www.bioradiations.com/the-chromatography-chronicles-part-3-adding-swapping-and-scaling-better-chromatography-through-modular-design/ Tue, 10 Sep 2013 18:11:57 +0000 http://www.bioradiations.com/?p=1119 What does it take to configure your own chromatography system? Part three of The Chromatography Chronicles examines how the NGC™ chromatography system adapts and grows to meet changing lab needs, and the modular design that makes this possible.

The post The Chromatography Chronicles Part 3: Adding, Swapping, and Scaling — Better Chromatography through Modular Design first appeared on Bio-Radiations.]]> Buying a New Preparative Protein Purification System – You Have a New Choice https://www.bioradiations.com/buying-a-new-preparative-protein-purification-system-you-have-a-new-choice/ Tue, 13 Aug 2013 19:00:44 +0000 http://www.bioradiations.com/?p=2222 A day in the life of Maria, a post-graduate scientist. Both Maria and her PI are annoyed with their unreliable, out-dated protein purification system, and her PI asks Maria to buy them a new one. She struggles to find a system that meets all their needs until she remembers that Bio-Rad has a new next-generation chromatography system that will work for everyone!

The post Buying a New Preparative Protein Purification System – You Have a New Choice first appeared on Bio-Radiations.]]> The Chromatography Chronicles Part 2: The Chromatographers Who Came in from the Cold https://www.bioradiations.com/the-chromatography-chronicles-part-2-the-chromatographers-who-came-in-from-the-cold/ Tue, 13 Aug 2013 18:00:03 +0000 http://www.bioradiations.com/?p=1111 Is spending hours in the coldroom just an inevitable part of doing chromatography? The engineers and designers at Bio-Rad believed that, with a little innovation, the answer could be “no.” Part two of The Chromatography Chronicles reveals how they freed the NGC™ medium-pressure chromatography system — and its users — from the coldroom’s chilly confines.

The post The Chromatography Chronicles Part 2: The Chromatographers Who Came in from the Cold first appeared on Bio-Radiations.]]> Vote Yes for Better Chromatography — The Results Are In! https://www.bioradiations.com/vote-yes-for-better-chromatography-the-results-are-in/ Tue, 09 Jul 2013 19:00:16 +0000 http://www.bioradiations.com/?p=2231 Watch the protein purification election night special parody! Find out which chromatography system wins. Laugh Out Loud at the terrible protein purification "pundit" and Vote Yes for Better Chromatography "Fanatics." Learn what values and system features were appreciated most by voters.

The post Vote Yes for Better Chromatography — The Results Are In! first appeared on Bio-Radiations.]]> The Chromatography Chronicles Part 1: The Votes Are in — Customer Voices and the NGC Chromatography System Design Process https://www.bioradiations.com/the-chromatography-chronicles-part-1-the-votes-are-in-customer-voices-and-the-ngc-chromatography-system-design-process/ Tue, 09 Jul 2013 17:47:34 +0000 http://www.bioradiations.com/?p=1107 Whether through unreliability, lack of workflows, or poor design, chromatography systems can cause major hassles for any lab involved in purifying proteins. Would Bio-Rad be able to solve these longstanding problems? This new series takes an inside look at the development of the NGC medium-pressure chromatography system. This month: bringing customers into the design process.

The post The Chromatography Chronicles Part 1: The Votes Are in — Customer Voices and the NGC Chromatography System Design Process first appeared on Bio-Radiations.]]> Investigating Protein Translation with the NGC™ Chromatography System https://www.bioradiations.com/investigating-protein-translation-with-the-ngc-chromatography-system/ Thu, 14 Feb 2013 20:00:55 +0000 http://www.bioradiations.com/?p=2237 The Fraser lab at University of California Davis tested Bio-Rad’s NGC system for the purification of ribosomal complex proteins in their studies of protein translation mechanisms. The NGC system enabled these researchers to significantly increase their protein purification throughput and save valuable time in the lab.

The post Investigating Protein Translation with the NGC™ Chromatography System first appeared on Bio-Radiations.]]> Investigating Protein Translation with Bio-Rad’s NGC Chromatography System https://www.bioradiations.com/investigating-protein-translation-with-bio-rads-ngc-chromatography-system/ Fri, 08 Feb 2013 04:51:44 +0000 http://www.bioradiations.com/?p=666 The Fraser Laboratory in the Dept. of Molecular and Cellular Biology at UC Davis studies protein translation mechanisms by purifying diverse ribosomal proteins and reconstructing the translation complex in vitro. The lab utilized Bio-Rad’s NGC system to overcome specific purification difficulties with their protein while beta-testing the equipment. The NGC system enabled the group to increase their throughput by 25% and also saved substantial training time.

The post Investigating Protein Translation with Bio-Rad’s NGC Chromatography System first appeared on Bio-Radiations.]]> Stellan Hjertén Wins the California Separations Science Society Award https://www.bioradiations.com/stellan-hjerten-wins-the-california-separations-science-society-award/ Fri, 21 Oct 2011 23:52:25 +0000 http://www.bioradiations.com/?p=6345 This year the California Separation Science Society will present its annual Scientific Achievement Award to Professor Stellan Hjertén of Uppsala University. The award dinner will be held November 3, 2011 at the Waterfront Hotel in Jack London Square.

The post Stellan Hjertén Wins the California Separations Science Society Award first appeared on Bio-Radiations.]]> C-96 Autosampler for Reliable Protein Purification Sample Application https://www.bioradiations.com/c-96-autosampler-for-reliable-protein-purification-sample-application/ Wed, 15 Jun 2011 19:00:34 +0000 http://www.bioradiations.com/?p=3296 The C-96 autosampler improves the efficiency of protein purification workflows by enabling automated sample application. The straightforward tray designs are easy to use; the standard tray accommodates 84 sample vials (5 ml each) plus three additional vials (10 ml each) for mixing and dilution protocols.

The post C-96 Autosampler for Reliable Protein Purification Sample Application first appeared on Bio-Radiations.]]> Nuvia™ S Media — A High-Capacity Cation Exchanger for Biomolecule Purification and Downstream Processing https://www.bioradiations.com/nuvia-s-media-a-high-capacity-cation-exchanger-for-biomolecule-purification-and-downstream-processing/ Thu, 09 Jun 2011 19:00:58 +0000 http://www.bioradiations.com/?p=2926 Nuvia S media is a high-capacity strong cation exchanger (CEX) developed to operate over a range of experimental conditions, allowing scientists to scale up from laboratory- to process-scale more quickly and easily. Our data demonstrate that the high capacity and superb purification of Nuvia S media can significantly improve productivity by offering the robustness, capacity, and speed for processing large load volumes with smaller columns and less buffer consumption.

The post Nuvia™ S Media — A High-Capacity Cation Exchanger for Biomolecule Purification and Downstream Processing first appeared on Bio-Radiations.]]>