Real-time qPCR/PCR | Bio-Radiations

qPCR vs. ddPCR™ Assays: Select the Best Gene Expression Analysis Method for Your Application

Bioradiations Staff — Tue, 11 Nov 2025 17:57:13 +0000

Unsure whether to choose quantitative PCR (qPCR) or Droplet Digital™ PCR (ddPCR) for gene expression analysis? Bio-Rad’s new objective study comparing the two methods shows that the best choice depends ...

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Navigating Change: Public Health Labs Are Adapting to New Regulations for Laboratory-Developed Tests

Bioradiations Staff — Tue, 13 May 2025 18:20:26 +0000

Labs face a growing compliance burden and increased uncertainty due to new regulations surrounding laboratory-developed tests (LDTs). The U.S. FDA's final rule and Supreme Court's recent decision to overturn the Chevron doctrine are creating a complex regulatory environment that challenges the ability of public health labs to rapidly respond to emerging pathogens. This article explores the implications of these changes and more.

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Maximizing PCR and qPCR Throughput with Automated Solutions

Bioradiations Staff — Tue, 11 Feb 2025 18:10:48 +0000

Automation in PCR and qPCR processes is revolutionizing laboratory workflows in regulated environments like biopharma and CROs. By enhancing efficiency, reducing manual errors, and ensuring compliance with regulatory standards, automation allows labs to scale operations and increase throughput without compromising data quality. Here we show the range of Bio-Rad products that are compatible with automated solutions for PCR and qPCR workflows.

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Assay Controls and Reference Gene Selection are Essential Elements of Your Real-Time PCR Reactions

Bioradiations Staff — Tue, 13 Aug 2024 21:26:02 +0000

For a successful real-time PCR experiment, mastering assay controls and reference gene selection are critical. These key steps in your experimental workflow pave the way for reliable data and help you unlock the full potential of real-time PCR.

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Faster PCR and Flow Cytometry Highlighted in Annual Lab Automation Roundup

Bioradiations Staff — Tue, 13 Aug 2024 19:53:00 +0000

The annual international Society for Laboratory Automation and Screening (SLAS) conference is the highlight of the year for scientists in academia, industry, and government focused on leveraging the power of automation to achieve scientific breakthroughs. Here are some highlights of this year’s conference and how Bio-Rad contributed to it.

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PrimePCR Assays: Your Path to a Custom PCR Plate

Bioradiations Staff — Tue, 11 Jun 2024 18:41:24 +0000

Discover the simplicity of crafting your own custom PrimePCR plate with Bio-Rad. Follow along with our intuitive plate wizard, which helps you select your plate type (96- or 384-well) and compatible qPCR instrument for precise assay delivery. Watch this video to see how.

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Nature’s Lipid Nanoparticles: Challenges and Opportunities in Isolation and Analysis of Extracellular Vesicles

Bioradiations Staff — Tue, 11 Jun 2024 18:39:49 +0000

In nature, mammalian extracellular vesicles (EVs) are vital for intercellular communication, immune responses, and cell removal. EVs also hold promise in diagnostics, therapeutics, and vaccine delivery due to their abundance and natural role as carriers. Explore how overcoming challenges in EV purification and precise RNA analysis methods can help unlock their potential applications.

The post Nature’s Lipid Nanoparticles: Challenges and Opportunities in Isolation and Analysis of Extracellular Vesicles first appeared on Bio-Radiations.

Guidance for the Validation of qPCR and ddPCR Assays in Regulated Environments

Bioradiations Staff — Tue, 11 Jun 2024 17:46:49 +0000

In this webinar, learn about validating qPCR and ddPCR assays in regulated environments. Explore validation parameters and regulatory considerations.

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NalaGenetics Moves Pharmacogenomics from the Bench to the Clinic using qPCR: An Interview with Astrid Irwanto, Co-Founder and COO

Bioradiations Staff — Mon, 12 Feb 2024 18:14:12 +0000

Astrid Irwanto is the co-founder and COO of NalaGenetics, a company taking a novel, personalized approach to understanding genetic susceptibility to drug reactions in Southeast Asian populations. A PhD in human genetics and postdoctoral research in genetics disease, along with personal experiences, fuel Astrid’s pursuits. In this interview, we discuss NalaGenetics’ approach to pharmacogenomics and why Astrid decided to translate her academic research to a molecular diagnostics company.

The post NalaGenetics Moves Pharmacogenomics from the Bench to the Clinic using qPCR: An Interview with Astrid Irwanto, Co-Founder and COO first appeared on Bio-Radiations.

7 Ways Custom PCR Reagents and Plastics Fuel Product Development

Bioradiations Staff — Tue, 14 Nov 2023 20:03:09 +0000

One-size-fits-all solutions cannot address the full range of molecular biology needs in areas such as biotech, biopharma, or molecular diagnostics. When your requirements exceed the existing offerings of reagents and plastics on the market, Bio-Rad can partner with you and provide custom solutions. For a deeper look into these customization opportunities, we spoke with Anna Lai, manager of Bio-Rad's custom reagents program, and Jennifer Placek, who manages the company's custom plastics.

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PCR Plates: 5 Tips to Guide Your Buying Decision

Bioradiations Staff — Tue, 08 Aug 2023 18:13:21 +0000

Learn about 5 key aspects of a PCR plate that are crucial to your results. These tips provide you with the tools to make an informed decision when purchasing PCR plates.

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Introducing the PTC Tempo Thermal Cycler

Bioradiations Staff — Mon, 07 Aug 2023 21:04:11 +0000

The PTC Tempo Thermal Cycler represents the next generation of conventional PCR thermal cyclers from Bio-Rad. Engineered to maintain thermal performance, the PTC Tempo Thermal Cycler offers the flexibility to scale with your laboratory. The design features a refreshed, intuitive user interface and flexible connectivity options for streamlining protocol management. A motorized lid enables automation, taking your PCR to the next level with modern instrument control.

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Connected Instruments in the Digital Laboratory

Bioradiations Staff — Wed, 13 Jul 2022 04:50:00 +0000

As technology advances, a digital transformation has begun in laboratories around the world. Driven by the need to improve data quality and increase the value derived from the investment in generating those data, data management systems, devices, and their workflows are becoming more connected and integrated, ushering in the age of the digital laboratory. The digital laboratory is one where paper lab notebooks are replaced with fully digital data, complete with rich metadata, and generated using integrated workflows. Such data are easy to find, reuse, and reproduce. Further digitization and workflow integration to operate laboratory instruments will advance the burgeoning digital laboratory.

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The CFX Duet Real-Time PCR System: Our Most Accessible qPCR Technology

Bioradiations Staff — Wed, 13 Jul 2022 02:43:34 +0000

Through the CFX Duet Real-Time PCR System, our newest generation of quantitative PCR (qPCR) instrumentation, this technology is now even more accessible for molecular biologists and other professionals doing essential qPCR. With CFX Maestro Software, you can perform duplex detection, gene expression, and genotyping, all without paying for additional optics you don’t need. Check out our video tour of the new CFX Duet System.

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Beyond the Spec Sheet: Evaluating New qPCR Instrumentation

Bioradiations Staff — Tue, 14 Sep 2021 16:36:05 +0000

Whether you are part of a small academic lab or a multinational corporation, upgrading to a new quantitative PCR (qPCR) system can be both exciting and daunting. How will the new system perform compared to your current platform? Will your existing workflows translate smoothly? Read on to learn about our recent bridging study for users considering upgrading to the new CFX Opus Real-Time PCR System.

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Can You Find All the qPCR-Related Words?

Bioradiations Staff — Tue, 14 Sep 2021 16:31:44 +0000

Feeling stressed? Take a break with this qPCR-themed word search.

The post Can You Find All the qPCR-Related Words? first appeared on Bio-Radiations.

Countering COVID-19 with the Launch of Two New RT-PCR Kits

Bioradiations Staff — Mon, 08 Mar 2021 21:24:35 +0000

Bio-Rad is proud to introduce our new RT-PCR testing kits for COVID-19. These all-in-one kits are designed for sensitive, specific detection of SARS-CoV-2 alone, or simultaneous detection of SARS-CoV-2, influenza A, and influenza B. Read on to learn about each kit’s features.

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Introducing the Reliance Select cDNA Synthesis Kit

Bioradiations Staff — Tue, 08 Dec 2020 18:47:44 +0000

The Reliance Select cDNA Synthesis Kit is the newest reverse transcription product from Bio-Rad. This high-performance kit features Reliance Reverse Transcriptase, a novel chimeric enzyme that delivers superior results across a broad dynamic range, even with challenging samples. The Reliance Select cDNA Synthesis Kit delivers more accurate reverse transcription quantitative PCR (RT-qPCR) results, even from samples containing PCR inhibitors or degraded RNA.

The post Introducing the Reliance Select cDNA Synthesis Kit first appeared on Bio-Radiations.

Introducing the CFX Opus Real-Time PCR Systems — A Modern Take on Bio-Rad Consistency

Bioradiations Staff — Wed, 09 Sep 2020 21:55:33 +0000

Coming soon: The next iteration in Bio-Rad’s tradition of qPCR excellence. The compact CFX Opus Real-Time PCR System is more connected, consistent, and easier to use than ever before, and is available in 96- and 384-well formats. The CFX Opus Systems launch with our new BR.io cloud platform and the latest version of CFX Maestro Software, for complete instrument control and data analysis capabilities.

The post Introducing the CFX Opus Real-Time PCR Systems —
A Modern Take on Bio-Rad Consistency first appeared on Bio-Radiations.

Reference Gene Selection Using PrimePCR Plates and CFX Maestro Software

Bioradiations Staff — Wed, 12 Feb 2020 21:13:21 +0000

Reference gene selection and validation are very important for gene expression studies that involve different samples or experimental conditions and therefore are absolutely necessary before any further exploration. See how PrimePCR Reference Gene Selection Panels and CFX Maestro Software provide an easy-to-use system with which to select appropriate reference genes.

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Protein Thermal Shift Assays Made Easy with Bio-Rad’s Family of CFX Real-Time PCR Detection Systems

Bioradiations Staff — Wed, 13 Nov 2019 21:12:22 +0000

Protein thermal shift assays enable quick and easy buffer optimization for increased protein stability. See how Bio-Rad’s family of CFX Real-Time PCR Detection Systems can measure protein thermal stability with higher throughput and more buffer systems than traditional circular dichroism detection.

The post Protein Thermal Shift Assays Made Easy with
Bio-Rad’s Family of CFX Real-Time PCR Detection Systems first appeared on Bio-Radiations.

Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR

Bioradiations Staff — Wed, 11 Sep 2019 18:17:59 +0000

One-step multiplex RT-qPCR is a technique used to rapidly quantify multiple targets directly from RNA in a single reaction. But proper optimization and validation is essential for its success. These five tips will have you designing primers and probes, selecting reporters, and validating and optimizing your experiments like a pro.

The post Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR first appeared on Bio-Radiations.

Sensitive Codetection of Viral-Pathogen RNA and DNA Using One-Step Multiplex RT-qPCR

Bioradiations Staff — Tue, 09 Jul 2019 23:29:07 +0000

RT-qPCR is a well-established method for the detection, quantification, and typing of different microbial agents. However, when dealing with the challenges of pathogen detection, not all RT-qPCR reagents are created equal. See how we used a novel one-step multiplex RT-qPCR supermix to demonstrate sensitive codetection of viral RNA and DNA targets in a multiplex setting.

The post Sensitive Codetection of Viral-Pathogen RNA and DNA
Using One-Step Multiplex RT-qPCR first appeared on Bio-Radiations.

End the Cycle of Repeated, Failed Experiments with a Robust Field Guide to qPCR

Bioradiations Staff — Wed, 12 Jun 2019 00:46:09 +0000

qPCR has become ubiquitous for nucleic acid detection and quantification and remains the gold standard for a wide array of applications. To help you publish high-quality, reproducible data that reflect true experimental conditions, we’ve developed this comprehensive guide to performing the ultimate qPCR experiment.

The post End the Cycle of Repeated, Failed Experiments with a Robust Field Guide to qPCR first appeared on Bio-Radiations.

New Product Feature: Multiplex RT-qPCR in One Step

Bioradiations Staff — Tue, 14 May 2019 20:14:19 +0000

Using RT-qPCR for RNA, though critical in several industries, is often met with challenges. Many current options don't allow for multiplexing or high-throughput operation. Bio-Rad's new Reliance One-Step Multiplex Supermix was designed to grant researchers the expanded, efficient capabilities they need in RT-qPCR. This article presents some of the performance data from the new supermix and describes how exceptional it is at multiplex detection.

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Discover Our Capabilities

Bioradiations Staff — Wed, 10 Apr 2019 18:16:19 +0000

From flow cytometry to chromatography to western blot imaging to PCR plastics, learn more about Bio-Rad and see what’s possible in your research.

The post Discover Our Capabilities first appeared on Bio-Radiations.

One for All — The Universal qPCR Reagent

Bioradiations Staff — Tue, 11 Dec 2018 20:29:18 +0000

Passive reference dyes are included in qPCR/RT-qPCR supermixes to compensate for pipetting errors and thermal and/or optical differences in real-time instruments. Our universal line of reagents eliminates the need for spiking in dyes or having to worry about how much ROX your instrument needs. See how your qPCR experiments can also generate more reliable data and be reproducible by anyone.

The post One for All — The Universal qPCR Reagent first appeared on Bio-Radiations.

Discover lncRNA: Understanding the Long Noncoding Transcriptome

Bioradiations Staff — Wed, 14 Nov 2018 20:04:35 +0000

Learn about the exciting new field of long noncoding RNA (lncRNA) and how this newly discovered class of RNA compares to messenger RNA (mRNA).

The post Discover lncRNA: Understanding the Long Noncoding Transcriptome first appeared on Bio-Radiations.

Preamplification and How it Can Be Used to Maximize qPCR Data Generation from Limited Samples

Bioradiations Staff — Wed, 14 Feb 2018 00:15:14 +0000

Incorporating preamplification into your qPCR experiments may seem daunting, but it is a powerful way to analyze targets from limited samples. The tips in this article will help you understand how preamplification can enhance your qPCR experiments and the considerations and implications for incorporating it into your workflow.

The post Preamplification and How it Can Be Used to Maximize qPCR Data Generation from Limited Samples first appeared on Bio-Radiations.

Parallel Analysis of RNA and Protein Expression Using SingleShot™ Cell Culture Lysates

Bioradiations Staff — Thu, 10 Nov 2016 00:30:00 +0000

Comprehensive and effective gene expression studies evaluate both transcriptional and translational regulation. A streamlined protocol was developed for the parallel analysis of mRNA and protein levels using a single lysate produced from cultured cells.

The post Parallel Analysis of RNA and Protein Expression Using SingleShot™ Cell Culture Lysates first appeared on Bio-Radiations.

A Superior Method for Parallel Analysis of RNA and Protein Expression from Cell Culture Lysates

Bioradiations Staff — Thu, 16 Jun 2016 23:33:14 +0000

It is imperative to analyze both the transcriptional and translational profiles for a complete understanding of the functioning of genes. Here we describe a simple protocol that enables parallel analysis of both RNA and protein from single cell culture lysates. See how this workflow could save time and money and yield more reliable data than the traditional protocols used.

The post A Superior Method for Parallel Analysis of RNA and Protein Expression from Cell Culture Lysates first appeared on Bio-Radiations.

The Importance of Quality PCR Plastics

Bioradiations Staff — Wed, 11 May 2016 20:44:19 +0000

Do we ever think seriously about PCR plates, seals, and tubes as contributing to the quality of our gene expression results? Chances are that these PCR plastics are viewed just as labware by most people. In this article we bring out how simple factors, such as PCR plate rigidity, color of the wells, and adhesiveness of plate seals, can affect your gene expression results and show why it is important to use quality PCR plastics.

The post The Importance of Quality PCR Plastics first appeared on Bio-Radiations.

Using PrimePCR™ Gene Expression Assays to Understand Breast Cancer Metastasis

Bioradiations Staff — Tue, 08 Mar 2016 18:47:06 +0000

Tumor metastasis is a complex process. It requires the ability of the cancer cells to invade their surroundings and travel to distant sites and survive. Dr. Traci Lyons studies the mechanism of COX-2 functioning in the metastasis of breast cancer cells. Find out how Dr. Lyons used Bio-Rad’s PrimePCR™ Assays to confirm COX-2 knockdown at the mRNA level.

The post Using PrimePCR™ Gene Expression Assays to Understand Breast Cancer Metastasis first appeared on Bio-Radiations.

Using PrimePCR™ qPCR Assays to Understand Breast Cancer Metastasis

Bioradiations Staff — Tue, 08 Mar 2016 08:00:43 +0000

Traci Lyons describes how PrimePCR Assays ended her laboratory’s struggle to find a qPCR assay that could reliably detect transcription of the cyclooxygenase-2 (Cox-2) gene, which they had identified as potentially prometastatic. Using the PrimePCR Assay for Cox-2, researchers in her lab were finally able to verify cyclooxygenase-2 knockdown, which is a necessary step toward confirming its role in breast cancer metastasis.

The post Using PrimePCR™ qPCR Assays to Understand Breast Cancer Metastasis first appeared on Bio-Radiations.

New Product Feature: gDNA-Free cDNA in Two Simple Steps

Bioradiations Staff — Tue, 09 Feb 2016 22:08:23 +0000

Removing genomic DNA contamination from cDNA preparations is a challenge. All currently available methods are time consuming, could lead to sample loss, and are not efficient. Bio-Rad’s iScript™gDNA Clear cDNA Synthesis Kit tackles these issues and makes this step effortless. This article presents the performance data of the kit and describes how simple and efficient it is to use the kit for gDNA removal.

The post New Product Feature: gDNA-Free cDNA in Two Simple Steps first appeared on Bio-Radiations.

How to Design Hydrolysis Probes for qPCR

Bioradiations Staff — Wed, 13 Jan 2016 01:52:26 +0000

What's the optimal melting temperature for a hydrolysis probe? How long should your probe be? And why should you avoid 5' guanines like the plague? This episode answers these questions and gives you five simple tricks for designing the perfect hydrolysis probe.

The post How to Design Hydrolysis Probes for qPCR first appeared on Bio-Radiations.

So, How Can the Sso7d Fusion Polymerase Technology Help Your PCR?

Bioradiations Staff — Tue, 08 Dec 2015 19:10:10 +0000

During a PCR reaction, it is imperative for the DNA polymerase to remain attached to the template while extending the new strand. This association is jeopardized under challenging PCR conditions and when amplifying longer DNA fragments. See how Sso7d, a small protein capable of binding with the polymerase, helps in stabilizing this association and increasing PCR efficiency.

The post So, How Can the Sso7d Fusion Polymerase Technology Help Your PCR? first appeared on Bio-Radiations.

Six Tips for Increasing the Reproducibility of qPCR Experiments

Bioradiations Staff — Tue, 08 Dec 2015 19:02:53 +0000

Follow these six simple tips and you'll increase the reproducibility of your qPCR experiments.

The post Six Tips for Increasing the Reproducibility of qPCR Experiments first appeared on Bio-Radiations.

Bio-Rad’s PrimePCR™ Assays and Panels Eliminate Multiple Steps from Your qPCR Workflow

Bioradiations Staff — Tue, 10 Nov 2015 22:33:18 +0000

A qPCR workflow involves multiple steps, each of which adds to the time and cost of the entire experiment. The PrimePCR™ Assays and Panels from Bio-Rad eliminate a number of these steps with their predesigned primers and probes and help with the assay design. See what steps they eliminate and how that would help expedite your qPCR experiment.

The post Bio-Rad’s PrimePCR™ Assays and Panels Eliminate Multiple Steps from Your qPCR Workflow first appeared on Bio-Radiations.

How to Minimize Contamination in qPCR Experiments

Bioradiations Staff — Tue, 10 Nov 2015 22:16:42 +0000

Are you using ethanol to prep your bench for qPCR? Watch this episode to find out why that might be a bad idea. We're sharing seven tips for minimizing contamination of qPCR experiments.

The post How to Minimize Contamination in qPCR Experiments first appeared on Bio-Radiations.

How to Design Primers for qPCR

Bioradiations Staff — Wed, 14 Oct 2015 00:33:11 +0000

How long should a qPCR primer be and what types of sequences should you avoid? In this episode we share eight tips and tricks for primer design for your qPCR experiments.

The post How to Design Primers for qPCR first appeared on Bio-Radiations.

High Quality Gene Expression from Precious Samples — A Simplified Workflow

Bioradiations Staff — Thu, 11 Jun 2015 17:45:48 +0000

Obtaining enough starting material to complete multiple gene expression experiments can be challenging when working with precious and limited samples. Find out how Mark Kibschull of the Lunenfeld-Tanenbaum Research Institute in Toronto determines pluripotency and differentiation of limited human embryonic stem cells using Bio-Rad's SingleShot Cell Lysis Kit, PreAmp Supermix, and PrimePCR validated primer panels. This simplified workflow allows for the gene expression analysis of hundreds of genes from as little as 100 human embryonic stem cells, and without the cumbersome step of isolating and purifying RNA.

The post High Quality Gene Expression from Precious Samples
— A Simplified Workflow first appeared on Bio-Radiations.

Too Many Questions, Too Little Sample: Developing a Real-Time PCR Workflow for Monitoring Gene Expression in Limited Samples

Bioradiations Staff — Tue, 09 Jun 2015 22:42:57 +0000

Traditional real-time PCR workflows often limit the number of genes that can be studied, especially when sample amounts are small. A new workflow now allows Mark Kibschull of the Lunenfeld-Tanenbaum Research Institute in Toronto to track the expression of hundreds of genes using a fraction of the starting material required by traditional methods.

The post Too Many Questions, Too Little Sample: Developing a Real-Time PCR Workflow for Monitoring Gene Expression in Limited Samples first appeared on Bio-Radiations.

PrimePCR™ Pathway and Disease Panels – How to Modify a Predesigned Plate

Bioradiations Staff — Mon, 12 Jan 2015 20:00:45 +0000

One easy way to obtain a PrimePCR plate customized for your research is to modify one of Bio-Rad’s predesigned panels. This short video tutorial shows you how to use the online tools at Bio-Rad.com to edit a predesigned plate and create, then order your own customized 96- or 384-well PCR plate.

The post PrimePCR™ Pathway and Disease Panels – How to Modify a Predesigned Plate first appeared on Bio-Radiations.

How to Build a Custom PrimePCR™ Real-Time PCR Plate Using a Suggested or Blank Template

Bioradiations Staff — Mon, 12 Jan 2015 20:00:45 +0000

It’s fast and easy to build your own PrimePCR plate. This video walks you through using the Custom Plate Configurator at Bio-Rad.com to create your own 96- or 384-well PCR plate.

The post How to Build a Custom PrimePCR™ Real-Time PCR Plate Using a Suggested or Blank Template first appeared on Bio-Radiations.

How to Order PrimePCR™ Assays – Primers, Probes, and Panels

Bioradiations Staff — Tue, 09 Dec 2014 20:00:48 +0000

A wide range of PCR assays is available through Bio-Rad.com for use with real-time or Droplet Digital™ PCR systems. Get a quick refresher on ordering these products with this short video.

The post How to Order PrimePCR™ Assays – Primers, Probes, and Panels first appeared on Bio-Radiations.

Better, Faster, and Less Expensive Gene Expression Analysis Using Multiplex One-Step RT-qPCR: A Case Study of Colorectal Cancer

Bioradiations Staff — Tue, 10 Jun 2014 19:00:50 +0000

Conducting a triplex one-step RT-qPCR assay promises a more efficient and economical analysis of gene expression compared to running three individual assays. This technical report describes a colorectal cancer case study where a triplex multiplex assay was validated.

The post Better, Faster, and Less Expensive Gene Expression Analysis Using Multiplex One-Step RT-qPCR: A Case Study of Colorectal Cancer first appeared on Bio-Radiations.

Bio-Rad’s Universal Real-Time PCR App – Everything You Need to Know about Real-Time PCR at Your Fingertips

Bioradiations Staff — Thu, 15 May 2014 23:10:06 +0000

Bio-Rad’s Universal Real-Time PCR Kits and Reagents work on all real-time PCR systems. Now you can easily compare performance data and get supporting documents for these kits and reagents using Bio-Rad’s new tablet and web app. Learn more and download the app here.

The post Bio-Rad’s Universal Real-Time PCR App – Everything You Need to Know about Real-Time PCR at Your Fingertips first appeared on Bio-Radiations.

PrimePCR ddPCR CNV Assay Validation Data

Bioradiations Staff — Tue, 09 Jul 2013 21:46:29 +0000

Gene Name BRCA1 Gene Symbol BRCA1 Species Human Summary breast cancer 1, early onset [Source:HGNC Symbol;Acc:1100] Gene Aliases BRCC1:PPP1R53:RNF53 RefSeq Accession No. NM_007294.3:NM_007299.3:NM_007297.3:NM_007300.3 UniGeneID Hs.194143 Ensemble Gene ID ENSG00000012048 Entrez ...

The post PrimePCR ddPCR CNV Assay Validation Data first appeared on Bio-Radiations.

New PrimePCR™ Probe Assays for Real-time PCR and Droplet Digital™ PCR Technologies

Bioradiations Staff — Tue, 09 Jul 2013 18:26:52 +0000

Bio-Rad’s PrimePCR probe assays gave researchers a powerful, reliable set of investigative tools utilizing SYBR® Green detection chemistry. Now Bio-Rad is adding a number of new assays to the PrimePCR portfolio, bringing the benefits of PrimePCR assays to many new applications for both real-time PCR and Droplet Digital™ PCR platforms.

The post New PrimePCR™ Probe Assays for Real-time PCR and Droplet Digital™ PCR Technologies first appeared on Bio-Radiations.

T100™ Thermal Cycler – Endorsed by Customers around the World

Bioradiations Staff — Thu, 25 Apr 2013 19:00:20 +0000

Life science researchers all over the world use the T100 thermal cycler in their daily routine. In this video, users describe how it saves them time and helps them consistently obtain high-quality data.

The post T100™ Thermal Cycler – Endorsed by Customers around the World first appeared on Bio-Radiations.

Real-Time PCR Interactive Tutorials — From Reagent Selection to Real-Time PCR Data Analysis

Bioradiations Staff — Tue, 26 Mar 2013 22:42:42 +0000

Obtaining accurate quantitative PCR data depends on choosing and validating the proper reagents for a given experiment. These new tutorials present the knowledge base needed to understand the parameters involved in reagent selection in order to get the best results from real-time PCR procedures.

The post Real-Time PCR Interactive Tutorials — From Reagent Selection to Real-Time PCR Data Analysis first appeared on Bio-Radiations.

PrimePCR™ PCR Primers & Assays

Bioradiations Staff — Sat, 02 Mar 2013 00:04:49 +0000

PrimePCR™ assays for real-time PCR and Droplet Digital™ PCR are expertly designed and experimentally validated for guaranteed performance. Ways to order PrimePCR assays: My PrimePCR Reorder previously ordered assays PrimePCR ...

The post PrimePCR™ PCR Primers & Assays first appeared on Bio-Radiations.

Amplifying in the Outback: Researcher Brings Real-Time PCR to Australia’s Kimberley Wilderness

Bioradiations Staff — Mon, 04 Feb 2013 21:22:23 +0000

Dr. Tim Inglis of the University of Western Australia seeks better ways to conduct surveillance and respond to outbreaks of tropical infectious diseases. For over a decade he’s been developing detection assays for a slate of pathogens. Find out how Bio-Rad’s MiniOpticon Real-Time PCR system allows him to effectively run these assays in such remote locations as the Kimberley Wilderness in Australia’s Top End.

The post Amplifying in the Outback: Researcher Brings Real-Time PCR to Australia’s Kimberley Wilderness first appeared on Bio-Radiations.

New Premium Hard-Shell Microplate for Applied Biosystems 7500 Fast and ViiA 7 Fast Real-Time PCR systems

Bioradiations Staff — Mon, 07 Jan 2013 18:33:35 +0000

Bio-Rad introduces new Hard-Shell 96-well Semi-skirted low profile PCR plates for optimal performance with a variety of real-time PCR systems.

The post New Premium Hard-Shell Microplate for Applied Biosystems 7500 Fast and ViiA 7 Fast Real-Time PCR systems first appeared on Bio-Radiations.

New Roche LightCycler 480 Hard-Shell® Plate That Does Not Warp During Thermal Cycling

Bioradiations Staff — Fri, 14 Dec 2012 19:15:12 +0000

Stability during thermal cycling and uniformity of wells are important features in any PCR plate for obtaining reliable results. Bio-Rad’s new Hard-Shell® plates are stable, prevent warping and shrinkage, and are designed for optimal thermal transfer and recovery of samples.

The post New Roche LightCycler 480 Hard-Shell^® Plate That Does Not Warp During Thermal Cycling first appeared on Bio-Radiations.

New CFX96 Touch™ Deep Well Real-Time PCR Instrument Enables Large-Volume qPCR Reactions

Bioradiations Staff — Sun, 28 Oct 2012 20:32:09 +0000

Large sample volumes may sometimes be needed to conduct gene expression studies. Bio-Rad’s new CFX96 Touch Deep Well™ real-time PCR detection system can run sample volumes up to 125 µl and can discriminate up to five targets simultaneously.

The post New CFX96 Touch™ Deep Well Real-Time PCR Instrument Enables Large-Volume qPCR Reactions first appeared on Bio-Radiations.

CFX Qualification Plate — A Reliable Performance Verification Tool for any CFX Real-Time PCR System

Bioradiations Staff — Fri, 26 Oct 2012 18:28:28 +0000

Verifying the performance of your real-time PCR system is crucial to obtaining reliable data from your experiments. Bio-Rad’s new CFX qualification plate comes with a quick and easy protocol that provides reliable performance verification of any real-time qPCR system.

The post CFX Qualification Plate — A Reliable Performance Verification Tool for any CFX Real-Time PCR System first appeared on Bio-Radiations.

PrimePCR™ Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy

Bioradiations Staff — Wed, 24 Oct 2012 19:00:31 +0000

Pathway analysis is an efficient approach for studying a large number of related gene targets in a single experiment. Bio-Rad Laboratories and Thomson Reuters, a leading provider of system biology tools, have partnered to provide predesigned pathway panels for gene expression analyses using real-time qPCR. This tech report describes how Bio-Rad’s PrimePCR™ pathway panels were curated and designed using a ranking strategy developed by Thomson Reuters.

The post PrimePCR™ Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy first appeared on Bio-Radiations.

New Semi-Automated Heat Sealer Delivers Consistent and Reliable PCR Plate Sealing

Bioradiations Staff — Mon, 08 Oct 2012 19:09:57 +0000

The PX1 PCR Plate Sealer efficiently seals microplates used in thermal cycling. This heat sealing method consistently delivers reliable data by helping eliminate sample evaporation during PCR.

The post New Semi-Automated Heat Sealer Delivers Consistent and Reliable PCR Plate Sealing first appeared on Bio-Radiations.

PrimePCR™ Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation

Bioradiations Staff — Mon, 20 Aug 2012 19:00:20 +0000

The MIQE guidelines have set a new standard for publishing qPCR results to ensure integrity in the scientific literature and increase experimental transparency. One of the key guidelines focuses on the proper design and experimental validation of primer assays used in qPCR. This tech report describes the design and wet-lab validation of PrimePCR assays and how they comply with the MIQE guidelines.

The post PrimePCR™ Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation first appeared on Bio-Radiations.

New and Improved CFX Manager™ Software for Real-Time PCR Data Acquisition and Analysis

Bioradiations Staff — Mon, 06 Aug 2012 17:49:28 +0000

The latest version of the CFX Manager software provides a variety of usability enhancements and powerful new data analyses so users can get the most out of each run.

The post New and Improved CFX Manager™ Software for Real-Time PCR Data Acquisition and Analysis first appeared on Bio-Radiations.

Experimentally Validated PrimePCR™ Assays and Panels for Real-Time PCR

Bioradiations Staff — Fri, 29 Jun 2012 19:04:35 +0000

PrimePCR™ assays set a new standard for real-time PCR. Bio-Rad® collaborated with the leaders in real-time PCR to design, optimize, and experimentally validate every primer pair.

The post Experimentally Validated PrimePCR™ Assays and Panels for Real-Time PCR first appeared on Bio-Radiations.

Novel HRM Assays Expedite Drug Resistance Surveillance for Leprosy Research

Bioradiations Staff — Tue, 10 Apr 2012 18:22:14 +0000

The significant drop in leprosy worldwide is attributed to the development of antimicrobial multidrug therapy (MDT). Resistance to rifampicin — the backbone of MDT treatment — has appeared. Controlling leprosy transmission requires routine surveillance for mutations in the drug target genes before, during, and after the course of treatment. New high resolution melt (HRM) assays offer leprosy researchers a faster, more economical method to investigate resistance targets.

The post Novel HRM Assays Expedite Drug Resistance Surveillance for Leprosy Research first appeared on Bio-Radiations.

PrimePCR™ Lookup Tool

Bioradiations Staff — Fri, 02 Mar 2012 23:31:29 +0000

Use the PrimePCR lookup tool to find assays and panels for your genes of interest.

The post PrimePCR™ Lookup Tool first appeared on Bio-Radiations.

Introducing iTaq Universal Supermixes for Highest Quality qPCR Results on Any Instrument

Bioradiations Staff — Fri, 24 Feb 2012 18:21:47 +0000

The iTaq™ universal SYBR® Green supermix and iTaq™ universal probes supermix are ready-to-use 2x supermixes formulated for sensitive, efficient, and reproducible qPCR data on any instrument.

The post Introducing iTaq Universal Supermixes for Highest Quality qPCR Results on Any Instrument first appeared on Bio-Radiations.

Third Generation PCR

Bioradiations Staff — Thu, 09 Feb 2012 20:00:28 +0000

The McCarroll laboratory studies the biological effects of human genome polymorphism, seeking to define how genome variation influences gene expression and risk of disease.

The post Third Generation PCR first appeared on Bio-Radiations.

A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data

Bioradiations Staff — Thu, 15 Dec 2011 21:23:32 +0000

This tech report shows how qPCR can lead to erroneous conclusions regarding differences in MCM7 gene expression between normal and tumor human breast cancer samples if the key steps set out in the MIQE guidelines are not followed.

The post A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data first appeared on Bio-Radiations.

QX100™ Droplet Digital™ PCR system

Bioradiations Staff — Wed, 23 Nov 2011 20:42:43 +0000

Bio-Rad’s QX100™ Droplet Digital™ PCR system provides an absolute measure of target DNA molecules with unrivaled precision and accuracy.

The post QX100™ Droplet Digital™ PCR system first appeared on Bio-Radiations.

High-Throughput Automated Extraction of RNA Using the Aurum™ Total RNA 96 Kit

Bioradiations Staff — Thu, 16 Jun 2011 20:22:34 +0000

This tech report describes an automated method of RNA extraction using the Aurum total RNA 96 kit and its use in a magnetic–bead based automated process. Results show that throughput can be easily increased with the Aurum total RNA 96 kit and that the automation protocols yield more reliable results, particularly when high sample volumes are used.

The post High-Throughput Automated Extraction of RNA Using the Aurum™ Total RNA 96 Kit first appeared on Bio-Radiations.

Hard-Shell® 384-Well 480 PCR Plates

Bioradiations Staff — Tue, 14 Jun 2011 19:00:35 +0000

The proprietary warp-free Hard-Shell plates have a reputation as premium plates and were widely used on Bio-Rad, MJ, and Applied Biosystems instruments for the Human Genome Project. Now, researchers can use the same high-quality Hard-Shell plates on Roche LightCycler 480 instruments.

The post Hard-Shell^® 384-Well 480 PCR Plates first appeared on Bio-Radiations.

Guidelines for Developing Robust and Reproducible High-Resolution Melt Analysis Assays

Bioradiations Staff — Mon, 13 Jun 2011 19:00:40 +0000

Classifying and understanding genetic variation between populations and individuals is an important aim in the field of genomics. High resolution melt (HRM) analysis is the quantitative analysis of the melt curve of a DNA fragment following amplification by PCR and can be considered the next-generation application of amplicon melting analysis. Careful sample preparation and planning of experimental and assay design are crucial for robust and reproducible results.

The post Guidelines for Developing Robust and Reproducible High-Resolution Melt Analysis Assays first appeared on Bio-Radiations.

All cDNA Synthesis Kits Are Not Created Equal: RNase A Inhibition and Its Effect on qPCR Data

Bioradiations Staff — Thu, 09 Jun 2011 19:00:40 +0000

Not all cDNA synthesis kits have the same capacity to protect the integrity of RNA. An optimal level of RNase inhibitor is critical for powerful RNase inhibition and efficient reverse transcription. The iScript™ cDNA synthesis kit comes with a 5x iScript reaction mix and iScript reverse transcriptase, which is also blended with a potent RNase inhibitor.

The post All cDNA Synthesis Kits Are Not Created Equal: RNase A Inhibition and Its Effect on qPCR Data first appeared on Bio-Radiations.

Research Applications for the EpiQ™ Chromatin Analysis Kit

Bioradiations Staff — Wed, 08 Jun 2011 19:00:31 +0000

EpiQ chromatin analysis kit is a novel epigenetics research tool for the quantitative assessment of chromatin structure in cultured cells. By combining in situ chromatin digestion, genomic DNA purification, and real-time PCR, the chromatin state for several gene promoters can be studied simultaneously.

The post Research Applications for the EpiQ™ Chromatin Analysis Kit first appeared on Bio-Radiations.

The T100™ Thermal Cycler

Bioradiations Staff — Wed, 01 Jun 2011 19:00:52 +0000

Why the T100 thermal cycler is the smart choice for your PCR.

The post The T100™ Thermal Cycler first appeared on Bio-Radiations.

1000-Series Touch Thermal Cyclers

Bioradiations Staff — Wed, 01 Jun 2011 19:00:00 +0000

Touch the future of PCR; introducing the 1000-series Touch thermal cyclers.

The post 1000-Series Touch Thermal Cyclers first appeared on Bio-Radiations.