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mAb Purification Process Development Using Mixed-Mode Resins — The DOE Approach

In this case study, process chromatography application scientist Artur Stanczak presents a two-step mAb purification workflow, comparing multimodal resin alternatives used for the polishing step that occurs after protein A capture. The design of experiment (DOE) data presented characterizes the behavior of multiple mAbs with two different mixed-mode resins. The importance of scale, prior knowledge, and the stage of development will also be discussed. The results of both mixed-mode resin studies and the different DOE approaches demonstrate the ability to achieve high-purity products.
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Chromatography VideoVideos

Efficient and Rapid Purification of E. coli–Expressed Toxin Recombinant Protein Fragments

Tetanus toxoid (TT) is a common carrier protein for conjugate vaccines, but its production is faced with significant challenges. Learn about a purification process that yields over 98% pure target protein in this video.
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