ArticlesProtein Interaction AnalysisTechnical Reports

Highly Efficient Lipoparticle Capture and SPR Binding Kinetics of a Membrane Protein Using the ProteOn™ XPR36 Protein Interaction Array System

The genes coding for membrane proteins, such as G-protein coupled receptors (GPCR), ion channels, and other membrane-bound enzymes make up 25–30% of the human genome and membrane proteins are estimated to represent 30–40% of the top drug targets. There is growing research, industrial, and commercial interest in the study of membrane proteins in general, and more specifically in immobilizing membrane proteins to various biosensor surfaces.
ArticlesReal-time qPCR/PCRTechnical Reports

A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data

This tech report shows how qPCR can lead to erroneous conclusions regarding differences in MCM7 gene expression between normal and tumor human breast cancer samples if the key steps set out in the MIQE guidelines are not followed.
ArticlesProduct HighlightsReal-time qPCR/PCR

QX100™ Droplet Digital™ PCR system

Bio-Rad’s QX100™ Droplet Digital™ PCR system provides an absolute measure of target DNA molecules with unrivaled precision and accuracy.
ArticlesBio-Plex Multiplex AssaysProduct Highlights

Bio-Rad Introduces New Bio-Plex® Human Cancer Assays

Bio-Plex Pro™ Human Cancer Biomarker Panel 1 is a unique blend of magnetic bead-based assays designed to meet the needs of the most discerning preclinical and clinical researchers.
ArticlesElectrophoresis/Western BlottingProduct Highlights

Using the new PROTEAN® i12™ IEF System to Achieve Highly Reproducible Data and Extended Separation Over Multiple pH Ranges in a Single Run

2-D electrophoresis is a multistep technique that separates proteins first by a protein’s isoelectric point and then by its molecular weight. This powerful technique can separate thousands of proteins in a complex proteome and is useful for applications such as protein profiling and biomarker discovery. The drawback of the technique is that it requires multiple steps, each of which can be time-consuming and can contribute to problems with reproducibility.
ArticlesElectrophoresis/Western BlottingTechnical Reports

Use of the PROTEAN® i12™ IEF System for In-Gel Peptide Fractionation Prior to LC-MS and Comparison with Off-Gel Fractionation

This tech report compares in-gel and off-gel separation of proteins in terms of overall speed and simplicity of the workflow, resolution of the separation, and depth of proteome coverage provided. Results found that as well as higher peptide yields, in-gel separations were better suited to take full advantage of downstream analysis methods.
ArticlesElectrophoresis/Western BlottingProduct Highlights

TGX Stain-Free™ Precast Gels

Mini-PROTEAN® TGX Stain-Free™ and Criterion™ TGX Stain-Free™ precast gels, when combined with Bio-Rad’s ChemiDoc™ MP or Gel Doc™ EZ imagers, provide researchers with the fastest protein separation and visualization available using the standard Laemmli system.
ArticlesFeatured Stories

Protein Expression and Purification Series — A Laboratory Course for Training Researchers

Protein Expression and Purification Laboratory Course for Training Researchers on Protein Expression and Purification.
ArticlesProduct HighlightsProtein Interaction Analysis

ProteOn™ HTE Sensor Chip

The HTE sensor chip is designed for label-free biomolecular interaction analysis with polyhistidine-tagged proteins. It features a novel tris-NTA (3-NTA) surface for excellent performance of polyhistidine-tagged protein capture, providing high binding stability and regeneracy. It is an ideal choice for the applications of protein-small molecule and other protein interactions requiring high sensitivity.
ArticlesChromatographyResearch Highlights

Stellan Hjertén Wins the California Separations Science Society Award

This year the California Separation Science Society will present its annual Scientific Achievement Award to Professor Stellan Hjertén of Uppsala University. The award dinner will be held November 3, 2011 at the Waterfront Hotel in Jack London Square.