ArticlesProtocols and TipsReal-time qPCR/PCR

Guidelines for Developing Robust and Reproducible High-Resolution Melt Analysis Assays

Classifying and understanding genetic variation between populations and individuals is an important aim in the field of genomics. High resolution melt (HRM) analysis is the quantitative analysis of the melt curve of a DNA fragment following amplification by PCR and can be considered the next-generation application of amplicon melting analysis. Careful sample preparation and planning of experimental and assay design are crucial for robust and reproducible results.
ArticlesElectrophoresis/Western BlottingFeatured Stories

Efficient Electrophoresis: Aiding Advances in Cardiomiopathy Research

The electrophoretic workflow has undergone few changes since it was solidified as a fundamental tool in life science research in the 1970s. Today, just like then, researchers need to prepare samples, load gels, then perform separation and further downstream analysis, including western blotting.
ArticlesCell BiologyCustomer Stories

Unlocking the Potential of Stem Cell Research Using the EpiQ™ Chromatin Analysis Kit

Bedtime stories in Dr David Schaffer’s — one of today’s pioneers in stem cell research — childhood home were often not standard fairy tales. With both parents in careers as biomedical researchers (his mother in drug development and clinical trials for a major pharmaceutical company and his father, in cardiovascular research and a pharmacology professor) much of the conversation as far back as Schaffer can remember centered on biology and science.
ArticlesElectrophoresis/Western BlottingResearch Highlights

Protein Quantitation Assays — Frequently Asked Questions

Protein quantitation is an important part of many workflows. The estimation or quantitation of the protein in a sample frequently allows researchers to understand the results from their work and make decisions on the subsequent steps. Presented are answers to some of the most frequently ask questions related to usage of Bio-Rad’s colorimetric assays for protein quantitation.
ArticlesElectrophoresis/Western BlottingProtocols and Tips

Using the Precision Plus Protein™ WesternC™ Standard as a QC Tool at Key Steps in Your Electrophoresis and Blotting Workflow

As a fundamental tool in protein research, it is critical that all steps of the electrophoresis workflow (from running gels to visualizing blots) are performed successfully to ensure accurate, reliable, and reproducible results. Protein standards are often used to monitor electrophoretic separation and transfer efficiency but do not always enable ladder visualization after blot development.
ArticlesChromatographyProduct Highlights

Nuvia™ S Media — A High-Capacity Cation Exchanger for Biomolecule Purification and Downstream Processing

Nuvia S media is a high-capacity strong cation exchanger (CEX) developed to operate over a range of experimental conditions, allowing scientists to scale up from laboratory- to process-scale more quickly and easily. Our data demonstrate that the high capacity and superb purification of Nuvia S media can significantly improve productivity by offering the robustness, capacity, and speed for processing large load volumes with smaller columns and less buffer consumption.
ArticlesReal-time qPCR/PCRTechnical Reports

All cDNA Synthesis Kits Are Not Created Equal: RNase A Inhibition and Its Effect on qPCR Data

Not all cDNA synthesis kits have the same capacity to protect the integrity of RNA. An optimal level of RNase inhibitor is critical for powerful RNase inhibition and efficient reverse transcription. The iScript™ cDNA synthesis kit comes with a 5x iScript reaction mix and iScript reverse transcriptase, which is also blended with a potent RNase inhibitor.
ArticlesCell BiologyTechnical Reports

Comparison between the TC10™ Automated Cell Counter and the Lactate Dehydrogenase (LDH) Assay to Assess Cellular Toxicity in vitro

This tech report presents the results of a comparative study between the TC10 automated cell counter and the classical LDH assay to measure cell viability in suspension cells. Results show that the TC10 cell counter can replace the LDH assay to measure both the viability of cell suspensions and the cytotoxic effects of various compounds.
ArticlesBio-Plex Multiplex AssaysProduct Highlights

Bio-Plex Pro™ Mouse Th17 Cytokines

Bio-Plex Pro™ mouse Th17 assays are magnetic bead–based multiplex assays that offer accurate and reproducible measurements of 20 target molecules in serum, plasma, and tissue culture sample types for mouse models. These assays have been developed to provide reliable performance with the flexibility required to meet all of your research needs.
ArticlesReal-time qPCR/PCRResearch Highlights

Research Applications for the EpiQ™ Chromatin Analysis Kit

EpiQ chromatin analysis kit is a novel epigenetics research tool for the quantitative assessment of chromatin structure in cultured cells. By combining in situ chromatin digestion, genomic DNA purification, and real-time PCR, the chromatin state for several gene promoters can be studied simultaneously.