Articles

Pathway analysis is an efficient approach for studying a large number of related gene targets in a single experiment. Bio-Rad Laboratories and Thomson Reuters, a leading provider of system biology tools, have partnered to provide predesigned pathway panels for gene expression analyses using real-time qPCR. This tech report describes how Bio-Rad’s PrimePCR™ pathway panels were curated and designed using a ranking strategy developed by Thomson Reuters.

The PX1 PCR Plate Sealer efficiently seals microplates used in thermal cycling. This heat sealing method consistently delivers reliable data by helping eliminate sample evaporation during PCR.

When Dr. Fred Bauzon started his research, he worked with bioinformatics specialists to functionalize suitable gene candidates based on their expression levels with HMGCR transcript levels. All his experiments demonstrated a reduction in low-density lipoprotein receptor protein (LDLR) and candidate gene protein levels after the candidate gene was silenced. Here Bauzon discusses how TGX Stain-free precast gels have aided his research toward future drug targets for the therapeutic control of LDL uptake.

Bio-Rad’s new TC20 automated cell counter is compatible with broad range of cell sizes and types such as cell lines, primary cells (from tissue or blood), and stem cells.

Bio-Rad’s new Clarity Western ECL substrate provides high sensitivity, long signal duration, and low background even at long exposures.

Bio-Rad’s latest semi-dry transfer system, the Trans-Blot® Turbo™, has changed the way researchers think about western blot transfers.

The MIQE guidelines have set a new standard for publishing qPCR results to ensure integrity in the scientific literature and increase experimental transparency. One of the key guidelines focuses on the proper design and experimental validation of primer assays used in qPCR. This tech report describes the design and wet-lab validation of PrimePCR assays and how they comply with the MIQE guidelines.

alt Melanoma is an aggressive cancer where early detection is essential for patient survival. The Facchiano lab is developing new anti-proliferation molecules against melanoma and is working to identify new molecular markers at the very early stages of pathology. Here Dr Antonio Facchiano discusses the merits of using the Bio-Plex technology to measure dose-dependent changes in expression levels of growth and angiogenic factors in cell lysates and supernatants.

The latest version of the CFX Manager software provides a variety of usability enhancements and powerful new data analyses so users can get the most out of each run.

Learning to use analysis software can be time-consuming and following a manual can be challenging.