See how you can efficiently reduce identification of optimal protein purification conditions from five days to just 18 hours. All you need is a DOE model and the Multivariable Scouting tool and automation abilities of the NGC Chromatography System.
Single-Step Influenza and Dengue Virus Purification with Mixed-Mode CHT Ceramic Hydroxyapatite XT Media
See how you can use CHT XT Mixed-Mode Media to recover over 75% of your virus in a single purification step. All this while reducing contaminating proteins by over 90% and removing over 60% of dsDNA. Get simple, fast, and reproducible viral purification with CHT XT.
The formation of aggregates is a common but highly undesirable occurrence during monoclonal antibody purification. See how on-column aggregation is dependent on the type of resin used and how one particular high-resolution cation exchange resin allows for the lowest proportion of aggregate formation.
Comprehensive and effective gene expression studies evaluate both transcriptional and translational regulation. A streamlined protocol was developed for the parallel analysis of mRNA and protein levels using a single lysate produced from cultured cells.
A well-designed multidimensional (Multi-D) chromatography process, which utilizes optimized individual column methods, can help automate multistep protein purification processes. This can help you save time and increase productivity. See how you can use Multi-D chromatography to expedite the purification of polyhistidine-tagged proteins when using a typical capture, intermediate, and polish purification workflow.
Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison of CHT™ Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes
The separation of monomers from product-related impurities is quite challenging in process separations. It requires a thorough screening and an efficient resin to get efficiency in the purification process. Review the performance data of Bio-Rad’s mixed mode CHT™ Ceramic Hydroxapatite in achieving process separation efficiency and how it compares with various Capto media.
A Semi-Automated Quantitative PCR Approach to Assess Differential Anti-Apoptotic Gene Expression Patterns Associated with Prostate Cancer Progression
Gene expression analyses become complicated and extremely time consuming when large numbers of genes need to be analyzed. Here we present a semi-automated qPCR workflow with reduced setup and processing times using Bio-Rad’s PrimePCR™ Panels and CFX Automation System II Plate Handler.
Genome editing using tools such as CRISPR/Cas9 and TALEN has gained enormous popularity in recent days due to their ability to create precise deletions or insertions. However when the frequency of these gene edits are really small, an ultrasensitive method like ddPCR™ is needed for accurate quantification. This technote explains how Droplet Digital™ PCR is used for the detection of HDR and NHEJ alleles generated using CRISPR/Cas9 and TALEN editing systems.
Advantages of Multidimensional (Multi-D) Chromatography Using the NGC™ Chromatography System over Traditional Sequential Chromatography
Traditional sequential chromatography typically requires intensive hands-on user manipulation, which could hinder the reproducibility of protein purification runs. Multi-D chromatography aims to improve this process while maintaining the efficacy of the traditional workflow.
Bio-Rad’s Bio-Plex Pro Human Inflammation Assay measures 37 of the most relevant inflammation biomarkers, including TNF superfamily proteins, Treg cytokines, IFN proteins, and matrix metalloproteinases, in a single multiplex panel.Check out the performance characteristics of the assay panel in this report.