In-Cell Western: A Versatile Tool for Quantitative Analysis of Protein Expression In-Situ
Protein expression analysis is essential in cell biology research, as it provides valuable insights into the molecular mechanisms that underlie cellular functions. Traditional methods for protein analysis, such as western blotting and ELISA, are useful for studying protein expression in cell lysates. However, these methods require labor-intensive sample preparation and do not provide information on the spatial distribution of proteins within intact cells. In-Cell Western is a powerful technique that enables quantitative and qualitative in situ protein expression analysis.
Across the Blot: A Western Blotting Crossword
Do you think you know western blotting? Test your knowledge with a crossword puzzle.
Resolved — Comic Series
Follow along as a new starry-eyed graduate student learns the ins and outs of life as a research scientist, gaining valuable western blotting tips and tricks. Along for the ride are a seasoned postdoctoral fellow and traditional principal investigator.
Introducing the GelDoc Go Imaging System
The next iteration of Bio-Rad Gel Documentation Systems has arrived. The GelDoc Go Imaging System gives you a benchtop imaging solution that enables gel documentation in three simple steps. Acquire high-resolution, publication-quality images of nucleic acid gels, protein gels, and colorimetric blots. Image up to four mini gels at once with the large imaging area, and easily set up and control the GelDoc Go Imaging System with Image Lab Touch Software. Read more about the compact, easy-to-use GelDoc Go System.
Stain-Free Western Blotting: Faster Results, Better Data
Western blotting is a well-accepted technique which has a place in every molecular biologist’s toolkit. However, the traditional process is lengthy and filled with manual steps, which can result in variability. Stain-Free technology can shorten your workflow and improve your data. Read our top three reasons to incorporate Stain-Free technology in your western blotting workflow.
Western Blot University
Join us for an in-depth dive into western blotting. Whether you are new to western blotting or an experienced user looking for tips, this five-part webinar series has something for you. Slides and downloadable resources will be available for each webinar. Complete all five classes to receive a certificate of completion and a colorful Western Blotting University poster.
Fluorescent Immunoblots and Multiplex Analysis
Presented by: Gary F. Ross, PhD, Proteomics Field Application Scientist
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Learn best practices for fluorescent western blotting, including multiplex analysis and image acquisition.
Advancements in Western Blotting Technology: A Western Workflow That Delivers More Precise, Accurate, and Reproducible Results
Presented by: Sean C. Taylor, MBA, PhD, Field Application Scientist Manager
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Learn how to produce journal-ready reproducible and quantitative western blot data.
10 Tips for Western Blot Detection of Phosphorylation Events
Being able to interrogate protein phosphorylation events and aberrant phosphorylation levels is critical to our understanding of various biological and pathological processes. However, detection of phosphorylated proteins in western blots can be challenging. These ten tips will help you generate robust data and cleaner blots.
See the Signal — Illuminating the Pathway to Confident Western Blot Detection of Phosphorylated Proteins
Presented by: Rachael Preston, PhD, Applications Scientist, Bio-Rad Laboratories
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The most common method for assessing the phosphorylation state of a protein is western blotting. In this webinar we talk you through how to fine-tune your western blot protocol to detect phosphorylation events and generate publication-quality western blots.