Finding a Good Antibody: Part I — Selecting the Best Antibodies.
ArticlesCell BiologyElectrophoresis/Western BlottingProtocols and Tips

Finding a Good Antibody:
Part I — Selecting the Best Antibodies

Even though antibodies are central to basic research as well as drug development and diagnostics, concerns with antibody validation remain high, and finding an antibody that works well for a specific application can be quite a challenge. Use these tips to select the best antibodies for your application.
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The How and Why of Normalizing Your Western Blots
ArticlesElectrophoresis/Western BlottingFeatured Stories

The How and Why of Normalizing Your Western Blots

Introducing data normalization into your western blotting workflow may sound unnecessary but it has proven to be a great way to ensure great results. Follow along as we break down some of the main takeaways from the first chapter in Dr. Oh’s popular wester blotting webinar series, including a unique method to ease normalization woes and worries.
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Electrophoresis/Western BlottingPast WebinarsWebinars

Generating Semi-Quantitative Western Blot Data Using Bio-Rad’s Image Lab™ 6.0 Software

Presented by: Kenneth J. Oh, PhD, Applications, Collaborations, and New Technology Manager, Protein Quantitation Marketing Group, Bio-Rad Laboratories, Inc.
Date: Tuesday, March 6, 2018

Western blotting is a popular tool for protein quantitation, and the advent of digital imaging has greatly increased the sensitivity, dynamic range, and quantitation limits of this technique. In the second webinar in this series, get step-to-step instructions for normalizing western blot data using Image Lab 6.0 Software.

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ArticlesElectrophoresis/Western BlottingProtocols and Tips

Tips to Make Fluorophore Picking Easier

Fluorescence is a property of photo-excitable molecules that has been exploited to develop dyes, or fluorophores, that are used in a variety of experimental procedures, including western blotting. Learn more about the science behind fluorescent dyes and get some helpful tips on how to pick the right fluorophores for your experimental needs.
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ArticlesElectrophoresis/Western BlottingGeneral Interest

Identify Major Immune Cell Types by Their CD Marker Expression

Our new immune cell lineage and marker database provides an interactive way to help you easily examine detailed marker data, view immune cell marker expression, and find the antibody to your marker. Learn more and try out the interactive tool for yourself.
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ArticlesElectrophoresis/Western BlottingGeneral Interest

Validating Antibodies — the Good, the Bad, and the Necessary

Despite the fact that antibodies are one of the most frequently used tools in life science and clinical research, there are no universally accepted guidelines to ensure that they have been properly validated. Learn about the issues facing researchers and what antibody suppliers and researchers can do to ensure that the research being carried out is trustworthy and is helping to improve overall data quality.
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ArticlesElectrophoresis/Western BlottingFeatured Stories

Fluorescent Western Blotting: Helpful Tips for Optimizing Your Protocol

Western blotting using fluorescently tagged detection antibodies is a technique that is gaining in popularity. However, adopting a new technique can be challenging. Use the tips provided in this article to get started and see how fluorescent western blotting can enhance your scientific research.
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ArticlesElectrophoresis/Western BlottingFeatured StoriesGeneral Interest

The Fluorescent Way: Why Start Fluorescent Western Blotting Now?

Find out why fluorescent western blotting is gaining in popularity and how it compares to more widely used methods, such as chemiluminescence. From easier multiplexing to brighter fluorophores to easier method development, learn about the many advantages of fluorescent western blotting.
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ArticlesElectrophoresis/Western BlottingProtocols and Tips

Ten Tips for Improving Western Blot Detection of Immunoprecipitation Samples

Immunoprecipitation (IP) experiments and the detection of IP samples by western blotting can be quite challenging at times. The tips in this article help you set up and optimize your overall IP experimenta design.
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ArticlesElectrophoresis/Western BlottingProtocols and Tips

Six Tips for Efficient Transfer of Your Proteins

A good and complete transfer of proteins from gels to membranes is crucial for the success of western blotting. Here are six useful tips for successful transfer of proteins.
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