For a long time X-ray film has been the standard for imaging western blots, but this method has its limitations. Find out how 3 researchers are using the digital tools offered by the new ChemiDoc Touch Imaging System to beat these limitations and improve their blot images.
Take a quick look at Bio-Rad’s brighter Precision Plus Protein™ Dual Color Standard with this article comparing this standard to other available protein markers. Plus, find out why the ability to visualize a protein standard after reprobing matters to one researcher!
Hand casting polyacrylamide gels is not an easy task. It is laborious, needs lot of pre-planning and is cumbersome. Bio-Rad’s new TGX and TGX Stain-Free FastCast gel solutions not only address all these issues, but provide added benefits, such as increased speed, stain-free visualization, and quality control. Learn more about these new handcasting kits.
Biologics Analysis Workflow™ – Delivering Increased Throughput, Sensitivity, Reproducibility, and Automation for cGMP Laboratories
Evaluating potential protein therapies for purity and/or identity represents a vital and highly regulated step in biological drug development. Bio-Rad’s new Biologics Analysis Workflow™ offers a high-throughput, sensitive, and reproducible solution that is cGMP-compliant.
Twenty years after the discovery of the link between BRCA gene mutations and breast cancer, researcher Ryan Jensen of the Yale Medical School is looking into the interactions between the DNA repair protein RAD51 and BRCA proteins using Bio-Rad’s V3 Western Workflow.
Reliable, Streamlined 2-D Western Blot Workflow for Evaluation of Antibodies Developed for Detection of Host Cell Proteins
Removing host cell protein (HCP) contaminants represents an essential step in the production of biologics (therapeutic protein drugs), but has long been a time- and labor-intensive process. Here, we demonstrate a simpler, quicker, and more standardized workflow for evaluating anti-HCP antibodies using 2-D electrophoresis and western blotting.
Using Bio-Rad’s PDQuest Software to Generate a Match Rate between 2-D Electrophoresis and Western Blotting
2-D electrophoresis followed by immunodetection of protein spots using western blotting is adopted commonly in proteomics studies. Here we present step-by-step instructions for obtaining accurate overlaps between the gel and the blot using the PDQuest 2-D gel analysis software.
Bio-Rad’s 2-D electrophoresis workflow offers better results in a shorter time. Take a detailed look at this workflow by downloading the updated edition of our How-To Guide.
Bio-Rad’s innovative Gel Doc EZ imaging system features something unique that no other imaging system has – brains.
When Dr. Fred Bauzon started his research, he worked with bioinformatics specialists to functionalize suitable gene candidates based on their expression levels with HMGCR transcript levels. All his experiments demonstrated a reduction in low-density lipoprotein receptor protein (LDLR) and candidate gene protein levels after the candidate gene was silenced. Here Bauzon discusses how TGX Stain-free precast gels have aided his research toward future drug targets for the therapeutic control of LDL uptake.