ArticlesElectrophoresis/Western BlottingTechnical Reports

Use of the PROTEAN® i12™ IEF System for In-Gel Peptide Fractionation Prior to LC-MS and Comparison with Off-Gel Fractionation

This tech report compares in-gel and off-gel separation of proteins in terms of overall speed and simplicity of the workflow, resolution of the separation, and depth of proteome coverage provided. Results found that as well as higher peptide yields, in-gel separations were better suited to take full advantage of downstream analysis methods.
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ArticlesElectrophoresis/Western BlottingProduct Highlights

TGX Stain-Free™ Precast Gels

Mini-PROTEAN® TGX Stain-Free™ and Criterion™ TGX Stain-Free™ precast gels, when combined with Bio-Rad’s ChemiDoc™ MP or Gel Doc™ EZ imagers, provide researchers with the fastest protein separation and visualization available using the standard Laemmli system.
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ArticlesElectrophoresis/Western BlottingProduct Highlights

ChemiDoc™ MP Imaging System

The ChemiDoc MP imaging system enables stain-free operation and lets you visualize proteins at every stage of your experiment. Its flexibility and sensitivity are complemented by a simple, intuitive operation that integrates seamlessly into your workflow.
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ArticlesElectrophoresis/Western BlottingProduct Highlights

PROTEAN® i12™ IEF System

Introducing Bio-Rad’s PROTEAN i12 IEF system. Each channel in the i12™ focusing tray is powered by its own power supply, enabling precise control over each IPG strip. This makes it possible to run different sample types, different gradients, and multiple protocols all at the same time.
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ArticlesElectrophoresis/Western BlottingTechnical Reports

PROTEAN® i12™ IEF System: Independent Voltage and Current Control Enables Optimization of First-Dimension IEF Conditions

This tech report demonstrates the advantages of independently controlled voltage and current parameters for each focusing tray in optimizing IEF conditions for a complex, insoluble membrane protein fraction.
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ArticlesElectrophoresis/Western BlottingProduct Highlights

Immun-Blot® LF (Low Fluorescence) PVDF Membranes

Immun-Blot LF (low fluorescence) PVDF membranes are protein blotting membranes optimized for fluorescent and multiplex fluorescent applications. They offer high signal-to-background ratio, low autofluorescence, and superior protein retention to maximize blot detection sensitivity and enable downstream quantitation.
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ArticlesElectrophoresis/Western BlottingProduct Highlights

Using Precision Plus Protein™ WesternC™ Standards to Validate Major Steps in the Western Blotting Workflow

Western blotting, although a commonly used technique, involves a time-consuming multi step process. The whole western blotting workflow could take up to three days to complete. Thus, having a tool to monitor each of the major steps in the western blotting workflow reduces chances of finding out at the very end of the workflow that an experiment was not successful.
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ArticlesElectrophoresis/Western BlottingFeatured Stories

Gel Doc™ EZ Imaging System – A Tale of Speed and Simplicity in Two Cities

The Gel Doc™ EZ imaging system makes an impact from coast to coast — at the Wyss Institute for Biologically Inspired Engineering at Harvard University in Boston, and in the Geoff Rosenfeld Laboratory at the University of California, San Diego.
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ArticlesElectrophoresis/Western BlottingFeatured Stories

Simplicity in Imaging: From Conception to Launch

When it comes to gel imaging and blotting, conventional imaging devices have tended to offer either high-performance, complex functionality or low-cost, low-performance simplicity. That is, until now.
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ArticlesElectrophoresis/Western BlottingFeatured Stories

Efficient Electrophoresis: Aiding Advances in Cardiomiopathy Research

The electrophoretic workflow has undergone few changes since it was solidified as a fundamental tool in life science research in the 1970s. Today, just like then, researchers need to prepare samples, load gels, then perform separation and further downstream analysis, including western blotting.
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