Cell Biology VideoVideos

The ZE5 Cell Analyzer, a Fast and Flexible Flow Cytometer for High-Throughput Screening

High-throughput screening (HTS) is a critical component of the entire drug discovery process. Flow cytometry is an attractive alternative to imaging and plate reader assays for HTS in drug discovery, due to its increased sensitivity and multiplexing capabilities. The ZE5 Cell Analyzer eliminates pain points that have hindered the full adoption of flow cytometry for drug discovery.
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ArticlesDroplet Digital PCR (ddPCR)General Interest

Bispecific Antibodies and Cancer Immunotherapy

Bispecific antibodies (bsAbs) are an important addition to the immuno-oncology toolbox. Designed to recognize two distinct epitopes, bsAbs have enhanced binding, specificity, and efficacy compared to current monovalent antibody therapeutics, making them exciting candidates for more targeted cancer treatments. Learn about bsAbs and how our tools can help scientists with their research on therapeutic candidates.
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ArticlesCell Biology

5 Reasons to Read the Antibody Advice Guide

In the day-to-day pressures of a research environment, it is natural to want to generate important data as quickly as possible. However, there are real benefits in ensuring that the antibody you have picked is the best for your experiment and spending time aliquoting and titrating your antibodies. Bio-Rad recently launched a free Antibody Advice Guide to equip scientists with the knowledge to get the best out of their experiments with antibodies. Read on for five examples of valuable information that you will learn from the guide, helping you generate publication-ready data.
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ArticlesCell Biology

5 High-Throughput Screening Applications Using Flow Cytometry

For many scientists, high-throughput flow cytometry screens are associated with drug or compound libraries, but did you know that you can also apply this technique to study microorganisms, clinical melanoma samples, and even protein trafficking? Read on for five examples of the use of flow cytometry in diverse, high-throughput screening applications.
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ArticlesCell BiologyFeatured StoriesGeneral InterestProtocols and Tips

6 Tips to Secure Funding for Flow Cytometers

We asked core facilities staff and research scientists from the U.S. and Europe for their top tips for securing funding for large instrumentation, such as a cell analyzer or cell sorter. Here are the key questions you should consider while preparing your grant application to increase your chances of a successful outcome.
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ArticlesDrug Discovery and DevelopmentGeneral Interest

Advanced Cancer Therapeutics: Increasing Efficacy and Specificity and Reducing Toxicity

Research into human diseases has led to the development of novel monoclonal antibody drugs. These monoclonal antibody therapies have been successful because of their specificity and selectivity. However, ongoing development and successfully launching a new mAb drug require robust and reliable methods to screen for drug targets, demonstrate drug safety and efficacy, and scale up manufacturing to meet the stringent regulatory guidelines required for approval.
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ArticlesCell BiologyCOVID-19Drug Discovery and DevelopmentFeatured StoriesGeneral InterestResearch Highlights

Immune Surveillance in SARS-CoV-2 Vaccine Development

The ability to characterize the target of cell-mediated immune response is crucial during vaccine development. In studying the characterization of SARS-CoV-2 infection pathogenesis in humans, a research team identified neutralizing antibodies that provided context for vaccine antigen design. Discover how the ZE5 Cell Analyzer was used to detect activation-induced markers, measure intracellular cytokines, and identify spike protein–specific antibodies in a rapid, high-throughput manner.
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ArticlesCell BiologyResearch Highlights

How FACS, Genetic Screens, and the HAP1 Cell Line Uncover the Biological Mechanisms of Cancer

Genetic screens are powerful tools for finding genes associated with a particular phenotype. They play an especially important role in cancer research and are leveraged to identify predictive biomarkers that can be used to improve treatment efficacy or trace the progression of genetic alterations that ultimately lead to disease. The HAP1 cancer cell line model and fluorescence-activated cell sorting (FACS) bring efficiency to the development of genetic screens that is making them a force to be reckoned with.
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ArticlesCell BiologyProtocols and Tips

7 Tips for Purchasing Your Next Cell Sorter

Presented here are some tips to help you avoid buyer’s remorse and make an educated decision about which instrument to purchase. These tips provide you with the tools to make an informed decision when purchasing a cell sorter.
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ArticlesCell BiologyResearch Highlights

Sorting an Old Problem: Are Cells Stressed by Fluorescence-Activated Cell Sorting?

Fluorescence-activated cell sorting is a valuable tool for research, but could the physical forces of sorting stress the cells, altering their behavior and confounding experimental results? Recent research found that although cell sorting activated p38 MAPK stress signaling, no functional or structural changes were observable. The results indicate that jet-in-air systems such as the Bio-Rad S3e Cell Sorter do not alter cell behavior.
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