PrimePCR™ Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation
The MIQE guidelines have set a new standard for publishing qPCR results to ensure integrity in the scientific literature and increase experimental transparency. One of the key guidelines focuses on the proper design and experimental validation of primer assays used in qPCR. This tech report describes the design and wet-lab validation of PrimePCR assays and how they comply with the MIQE guidelines.
New and Improved CFX Manager™ Software for Real-Time PCR Data Acquisition and Analysis
The latest version of the CFX Manager software provides a variety of usability enhancements and powerful new data analyses so users can get the most out of each run.
Experimentally Validated PrimePCR™ Assays and Panels for Real-Time PCR
PrimePCR™ assays set a new standard for real-time PCR. Bio-Rad® collaborated with the leaders in real-time PCR to design, optimize, and experimentally validate every primer pair.
Novel HRM Assays Expedite Drug Resistance Surveillance for Leprosy Research
The significant drop in leprosy worldwide is attributed to the development of antimicrobial multidrug therapy (MDT). Resistance to rifampicin — the backbone of MDT treatment — has appeared. Controlling leprosy transmission requires routine surveillance for mutations in the drug target genes before, during, and after the course of treatment. New high resolution melt (HRM) assays offer leprosy researchers a faster, more economical method to investigate resistance targets.
PrimePCR™ Lookup Tool
Use the PrimePCR lookup tool to find assays and panels for your genes of interest.
Introducing iTaq Universal Supermixes for Highest Quality qPCR Results on Any Instrument
The iTaq™ universal SYBR® Green supermix and iTaq™ universal probes supermix are ready-to-use 2x supermixes formulated for sensitive, efficient, and reproducible qPCR data on any instrument.
Third Generation PCR
The McCarroll laboratory studies the biological effects of human genome polymorphism, seeking to define how genome variation influences gene expression and risk of disease.
A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data
This tech report shows how qPCR can lead to erroneous conclusions regarding differences in MCM7 gene expression between normal and tumor human breast cancer samples if the key steps set out in the MIQE guidelines are not followed.
QX100™ Droplet Digital™ PCR system
Bio-Rad’s QX100™ Droplet Digital™ PCR system provides an absolute measure of target DNA molecules with unrivaled precision and accuracy.
High-Throughput Automated Extraction of RNA Using the Aurum™ Total RNA 96 Kit
This tech report describes an automated method of RNA extraction using the Aurum total RNA 96 kit and its use in a magnetic–bead based automated process. Results show that throughput can be easily increased with the Aurum total RNA 96 kit and that the automation protocols yield more reliable results, particularly when high sample volumes are used.