The proprietary warp-free Hard-Shell plates have a reputation as premium plates and were widely used on Bio-Rad, MJ, and Applied Biosystems instruments for the Human Genome Project. Now, researchers can use the same high-quality Hard-Shell plates on Roche LightCycler 480 instruments.
Using Precision Plus Protein™ WesternC™ Standards to Validate Major Steps in the Western Blotting Workflow
Western blotting, although a commonly used technique, involves a time-consuming multi step process. The whole western blotting workflow could take up to three days to complete. Thus, having a tool to monitor each of the major steps in the western blotting workflow reduces chances of finding out at the very end of the workflow that an experiment was not successful.
Now you can optimize research expenditure by multiplexing only the targets you need to get the answers you’re seeking. This newly updated tool allows you to choose from within panels to build the perfect kit.
The Gel Doc™ EZ imaging system makes an impact from coast to coast — at the Wyss Institute for Biologically Inspired Engineering at Harvard University in Boston, and in the Geoff Rosenfeld Laboratory at the University of California, San Diego.
When it comes to gel imaging and blotting, conventional imaging devices have tended to offer either high-performance, complex functionality or low-cost, low-performance simplicity. That is, until now.
Classifying and understanding genetic variation between populations and individuals is an important aim in the field of genomics. High resolution melt (HRM) analysis is the quantitative analysis of the melt curve of a DNA fragment following amplification by PCR and can be considered the next-generation application of amplicon melting analysis. Careful sample preparation and planning of experimental and assay design are crucial for robust and reproducible results.
The electrophoretic workflow has undergone few changes since it was solidified as a fundamental tool in life science research in the 1970s. Today, just like then, researchers need to prepare samples, load gels, then perform separation and further downstream analysis, including western blotting.
Bedtime stories in Dr David Schaffer’s — one of today’s pioneers in stem cell research — childhood home were often not standard fairy tales. With both parents in careers as biomedical researchers (his mother in drug development and clinical trials for a major pharmaceutical company and his father, in cardiovascular research and a pharmacology professor) much of the conversation as far back as Schaffer can remember centered on biology and science.
Protein quantitation is an important part of many workflows. The estimation or quantitation of the protein in a sample frequently allows researchers to understand the results from their work and make decisions on the subsequent steps. Presented are answers to some of the most frequently ask questions related to usage of Bio-Rad’s colorimetric assays for protein quantitation.
Using the Precision Plus Protein™ WesternC™ Standard as a QC Tool at Key Steps in Your Electrophoresis and Blotting Workflow
As a fundamental tool in protein research, it is critical that all steps of the electrophoresis workflow (from running gels to visualizing blots) are performed successfully to ensure accurate, reliable, and reproducible results. Protein standards are often used to monitor electrophoretic separation and transfer efficiency but do not always enable ladder visualization after blot development.