A Resource for Life Science Research

Bio-Rad Introduces New Bio-Plex® Human Cancer Assays

By BioRadiations Staff

High Performance Multiplex Immunoassays for Cancer Research

Bio-Plex Pro™ Human Cancer Biomarker Panel 1 is a unique blend of magnetic bead-based assays designed to meet the needs of the most discerning preclinical and clinical researchers. The multiplex format enables robust and reproducible measurement of 16 soluble proteins involved in disease processes such as:

  • Angiogenesis
  • Metastasis
  • Cell proliferation
  • Cell adhesion/ migration
  • Apoptosis
  • Inflammation

The assays incorporate several new features to enhance both quality and ease of use:

  • Reagent kit II for reliable performance in serum and plasma samples
  • Optimized protocol for high precision and broad-assay working ranges
  • Assay quick guide to get you started right away
  • Two-level quality controls included with premixed kits for monitoring intra- and inter-run precision

The LLOQ, ULOQ, LOD, and inter-assay precision %CV are mean data determined from three independent multiplex assays in a serum-based matrix

The lower limit of quantitation (LLOQ), upper limit of quantitation (ULOQ), limit of detection (LOD), and inter-assay precision %CV are mean data determined from three independent multiplex assays in a serum-based matrix. Intra-assay %CV is derived from one representative assay. LLOQ and ULOQ are defined as the boundary standard curve points in which the performance specifications of individual standard points were met for a 10% intra-assay CV and recovery range of 80–120%. Data were generated using the magnetic workflow with the Bio-Plex Pro II wash station.
* Factor × NIBSC value (pg/ml) = Bio-Plex value (pg/ml). Factors are based on average % recovery of Bio-Plex standards (in the linear range) relative to NIBSC standards run on the same assay plate.

 

Levels of biomarkers in normal and cancer groups

Fig. 1. Levels of biomarkers in normal ( ) and cancer ( ) groups. A Student's t-test was used to determine statistical significance between groups. Black lines denote mean values; P values are indicated above each cancer population. Data analysis, graphing, and statistics were performed with Bio-Plex Data Pro™ software.

 

Ordering Information

Catalog#Description
Premixed, All-In-One Kit
171-AC500MBio-Plex Pro Human Cancer Biomarker Panel 1, 16-Plex, 1 × 96-well, includes premixed coupled magnetic beads, detection antibodies, standard, 2-level controls, detection antibody diluent, standard diluent HB, sample diluent HB, assay buffer, wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions for detecting 16 human cancer biomarkers all in one kit
Singleplex Sets*
171-BC501MBio-Plex Pro Human Cancer Biomarker Panel 1 sEGFR Set, 1 × 96-well
171-BC502MBio-Plex Pro Human Cancer Biomarker Panel 1 FGF-basic Set, 1 × 96-well
171-BC503MBio-Plex Pro Human Cancer Biomarker Panel 1 Follistatin Set, 1 × 96-well
171-BC504MBio-Plex Pro Human Cancer Biomarker Panel 1 G-CSF Set, 1 × 96-well
171-BC505MBio-Plex Pro Human Cancer Biomarker Panel 1 HGF Set, 1 × 96-well
171-BC506MBio-Plex Pro Human Cancer Biomarker Panel 1 sHER-2/neu Set, 1 × 96-well
171-BC507MBio-Plex Pro Human Cancer Biomarker Panel 1 sIL-6Ra Set, 1 × 96-well
171-BC508MBio-Plex Pro Human Cancer Biomarker Panel 1 Leptin Set, 1 × 96-well
171-BC509MBio-Plex Pro Human Cancer Biomarker Panel 1 Osteopontin Set, 1 × 96-well
171-BC510MBio-Plex Pro Human Cancer Biomarker Panel 1, PECAM-1 Set, 1 × 96-well
171-BC511MBio-Plex Pro Human Cancer Biomarker Panel 1 PDGF-AB/BB Set, 1 × 96-well
171-BC512MBio-Plex Pro Human Cancer Biomarker Panel 1 Prolactin Set, 1 × 96-well
171-BC513MBio-Plex Pro Human Cancer Biomarker Panel 1 SCF Set, 1 × 96-well
171-BC514MBio-Plex Pro Human Cancer Biomarker Panel 1 sTIE-2 Set, 1 × 96-well
171-BC515MBio-Plex Pro Human Cancer Biomarker Panel 1, sVEGFR-1 Set, 1 × 96-well
171-BC516MBio-Plex Pro Human Cancer Biomarker Panel 1, sVEGFR-2 Set, 1 × 96-well
Reagent Kits
171-304055Bio-Plex Pro Reagent Kit ll with Filter Plate, 1 × 96-well, includes detection antibody diluent, standard diluent HB, sample diluent HB, assay and wash buffers, streptavidin-PE, filter plate, and sealing tape, for vacuum separation methods
171-304055MBio-Plex Pro Reagent Kit ll with Flat Bottom Plate, 1 × 96-well, includes detection antibody diluent, standard diluent HB, sample diluent HB, assay and wash buffers, streptavidin-PE, flat bottom plate, and sealing tape, for magnetic separation methods
Standards
171-DC5000Bio-Plex Pro Human Cancer Biomarker Panel 1, 16-Plex Standards, pkg of 1 vial, lyophilized mixture of 16 standard antigens
171-DC5001Bio-Plex Pro Human Cancer Biomarker Panel 1, 16-Plex Standards, pkg of 50 lot-matched vials, lyophilized mixture of 16 standard antigens
Wash Stations and Accessories
300-34376Bio-Plex Pro Wash Station, includes magnetic plate carrier, waste bottle, 2 buffer bottles
300-34377Bio-Plex Pro II Wash Station, includes magnetic plate carrier, vacuum manifold plate carrier, waste bottle, 2 buffer bottles
171-025001Bio-Plex Pro Flat Bottom Plates, 40 × 96-well plates
171-304500Bio-Plex Wash Buffer, 1.5 L
171-304502Filter plate, pkg of 1, 96-well plate with clear plastic lid and tray, for Bio-Plex assays using the vacuum wash method, sealing tape not included
Software
171-001510Bio-Plex Data Pro™ with Bio-Plex Manager™ Software (5 seats), for multi-experiment analysis and advanced data visualization and Bio-Plex Manager Software (5 seats), for instrument data evaluation and optimization
171-001513Bio-Plex Data Pro Software (5 seats), for multi-experiment analysis and advanced data visualization
171-STND01Bio-Plex Manager Software (1 seat), for instrument data evaluation and optimization

* Singleplex sets include coupled magnetic beads and detection antibodies. They require Bio-Plex Pro reagent kit II and standards to run an assay.

 
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