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Purification is a multistep process toward confirmation of a target protein’s purity. In this webinar you will learn how to automate your protein purification workflow by following the creation of a hypothetical monoclonal antibody (mAb). Dr. Schaefer and Dr. Winship expertly demonstrate how the NGC Chromatography System and the AEX-500 series Autosampler work together to maximize screening capability as well as rapidly achieve therapeutic development objectives.

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Emerging reports from the COVID 19 pandemic include a spectrum of patient symptoms due to the host immune response. In this webinar, Bio-Rad scientists discuss early research and nascent hypotheses about the pathophysiology of SARS CoV 2–induced COVID 19 disease by evaluating cytokine and chemokine profiles, the role of chronic inflammation in comorbidities, and the arc of immune resolution of historical virulent pathogens, such as SARS and MERS.

Date: June 17, 2020
Time: 9 AM, U.S. Pacific time

• Robust methodology for viral titer using ddPCR
• Precise copy number determination & improved confidence in acceptance criteria
• Enhance your throughput and minimize hands-on time
• Learn how ddPCR is used in other key applications

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Dr. Drevland presents a viable alternative workflow for the development of a HUMIRA-like biosimilar using IEX and mixed-mode resins.

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Learn about evaluating viral clearance using a DOE approach with a mixed-mode chromatography resin.

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Learn best practices for fluorescent western blotting, including multiplex analysis and image acquisition.

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This webinar highlights the properties of Bio-Rad’s newest hydrophobic AEX chromatography resin, Nuvia aPrime 4A, which is designed to facilitate selective and reversible binding of target molecules for higher purity and recovery over traditional chromatography resins.

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Learn how to produce journal-ready reproducible and quantitative western blot data.

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This webinar provides an overview of multiplexing technology for research use with mouse samples and highlights the associated benefits to your drug discovery workflow. Specific topics include:

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The most common method for assessing the phosphorylation state of a protein is western blotting. In this webinar we talk you through how to fine-tune your western blot protocol to detect phosphorylation events and generate publication-quality western blots.

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Bio-Plex Manager Software is a powerful, intuitive tool used to generate and analyze xMap data. In this webinar you will:

• Learn how to create an assay protocol
• Learn how to acquire data
• Understand basic software parameters
• Learn about general functionality and navigation
• Master optimization of standard curves and export data for further analysis

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We discuss using a multiparameter flow cytometry panel to monitor T-cell exhaustion states and the expression of checkpoint inhibitors. We used an in vitro model; however, the panel is designed to be compatible with clinical samples to monitor immunotherapy.

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Learn about Nuvia HP-Q Resin, a new rigid bead engineered with large pores and optimized surface extenders to deliver both high binding capacity and high recovery at high flow rates. It is ideal for large molecule purification, including plasma proteins, viruses, and VLPs.

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Watch this webinar to learn about a new mixed-mode chromatography media used to purify biomolecules at process scale. It delivers unique selectivity and robust, uniformly sized beads with excellent pressure-flow properties ideal for bioprocess applications.

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Flow cytometry is a powerful technique used widely in biological research, but if you are just beginning your flow journey, it can be hard to know where to start. In this webinar, you will:

• Learn the important steps to obtaining better flow cytometry data
• Get advice on how to apply this knowledge to improve your experiments
• Find out how Bio-Rad’s panel builder tool can help you design your experiments, no matter how many colors you plan to use

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Learn how to quickly optimize conditions prior to automating a four-step purification of a histidine-tagged recombinant protein. Automating purification reduces a multi-day process into 12 hours while increasing yield and reproducibility.

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In this webinar, Yasha Talaga will explain how specific methodologies and instrument setup on the ZE5 Cell Analyzer allow for reliable exosome detection data.

Western blotting is an essential and ubiquitous method for protein research. We will discuss method optimization, data analysis best practices, and new advancements in imaging and fluorescent detection reagents that make western blot data more reproducible and quantitative.

Multiplex immunoassays are an extremely powerful tool for protein detection and quantitation. In this webinar, get a general overview of multiplex immunoassay technology and tips on setting up experiments to optimize your results.

Western blotting is a popular tool for protein quantitation, and the advent of digital imaging has greatly increased the sensitivity, dynamic range, and quantitation limits of this technique. In the second webinar in this series, get step-to-step instructions for normalizing western blot data using Image Lab 6.0 Software.

Western blotting continues to be a ubiquitous tool for the identification and quantitation of protein(s) of interest. The first webinar in this series will focus on common normalization techniques for quantitative western blotting and discuss important considerations when using these techniques.